Restriction map for pF1KB4546
 (Linear) (Six-base) MAPSORT of: KIBB4546.seq  Check: 2063  from: 1  to: 10380



KIBB4546.orf

 With 182 enzymes: SgfI * 

                        February 2, 2017 14:35  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     413     453    3134    3506    4719   10380 
   Size:        413      40    2681     372    1213    5661

  Fragments arranged by size: 

                5661    2681    1213     413     372      40
 
AccI GT'mk_AC

Cuts at:      0    1664    1868    5189    6377   10380 
   Size:       1664     204    3321    1188    4003

  Fragments arranged by size: 

                4003    3321    1664    1188     204
 
Acc65I G'GTAC_C

Cuts at:      0    3158    6320   10380 
   Size:       3158    3162    4060

  Fragments arranged by size: 

                4060    3162    3158
 
AclI AA'CG_TT

Cuts at:      0    6077   10380 
   Size:       6077    4303
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1576    2216    3309    3448    3510    3946    4532    5010 
   Size:       1576     640    1093     139      62     436     586     478

Cuts at:   5010    5474    5990    6766    8004    8099    8270    8683    8695 
   Size:        464     516     776    1238      95     171     413      12

Cuts at:   8695    9047   10380 
   Size:        352    1333

  Fragments arranged by size: 

                1576    1333    1238    1093     776     640     586     516
                 478     464     436     413     352     171     139      95
                  62      12
 
AfeI AGC'GCT

Cuts at:      0      41    8050   10380 
   Size:         41    8009    2330

  Fragments arranged by size: 

                8009    2330      41
 
AflII C'TTAA_G

Cuts at:      0    8598   10380 
   Size:       8598    1782
 
AflIII A'CryG_T

Cuts at:      0    1982    3104    7448    8093    9671   10380 
   Size:       1982    1122    4344     645    1578     709

  Fragments arranged by size: 

                4344    1982    1578    1122     709     645
 
AgeI A'CCGG_T

Cuts at:      0    1405    9727   10380 
   Size:       1405    8322     653

  Fragments arranged by size: 

                8322    1405     653
 
AhdI GACnn_n'nnGTC

Cuts at:      0    4653   10380 
   Size:       4653    5727
 
AleI CACnn'nnGTG

Cuts at:      0    6296   10380 
   Size:       6296    4084
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     728    7045   10380 
   Size:        728    6317    3335

  Fragments arranged by size: 

                6317    3335     728
 
AlwNI CAG_nnn'CTG

Cuts at:      0      14     784    2646    3187    3565    4244    5777    6914 
   Size:         14     770    1862     541     378     679    1533    1137

Cuts at:   6914    7987   10380 
   Size:       1073    2393

  Fragments arranged by size: 

                2393    1862    1533    1137    1073     770     679     541
                 378      14
 
ApaI G_GGCC'C

Cuts at:      0      21    5894    8560   10380 
   Size:         21    5873    2666    1820

  Fragments arranged by size: 

                5873    2666    1820      21
 
ApaLI G'TGCA_C

Cuts at:      0     117     562    2049    6274   10380 
   Size:        117     445    1487    4225    4106

  Fragments arranged by size: 

                4225    4106    1487     445     117
 
ApoI r'AATT_y

Cuts at:      0     465    1267    1445    1461    1609    2026    2507    2926 
   Size:        465     802     178      16     148     417     481     419

Cuts at:   2926    4825    5430    5689    6124    8318    8756   10311   10380 
   Size:       1899     605     259     435    2194     438    1555      69

  Fragments arranged by size: 

                2194    1899    1555     802     605     481     465     438
                 435     419     417     259     178     148      69      16
            
 
AseI AT'TA_AT

Cuts at:      0    6550   10380 
   Size:       6550    3830
 
AvaI C'yCGr_G

Cuts at:      0    3869    7528    9326    9974   10380 
   Size:       3869    3659    1798     648     406

  Fragments arranged by size: 

                3869    3659    1798     648     406
 
BamHI G'GATC_C

Cuts at:      0    4163    6108    7283    8791    9623   10380 
   Size:       4163    1945    1175    1508     832     757

  Fragments arranged by size: 

                4163    1945    1508    1175     832     757
 
BanI G'GyrC_C

Cuts at:      0    2879    3158    3494    4399    5300    5675    5964    6152 
   Size:       2879     279     336     905     901     375     289     188

Cuts at:   6152    6320    7021    7220    7745    9730   10380 
   Size:        168     701     199     525    1985     650

  Fragments arranged by size: 

                2879    1985     905     901     701     650     525     375
                 336     289     279     199     188     168
 
BanII G_rGCy'C

Cuts at:      0      21     546     906    2361    2462    4070    5894    7575 
   Size:         21     525     360    1455     101    1608    1824    1681

Cuts at:   7575    8560   10334   10380 
   Size:        985    1774      46

  Fragments arranged by size: 

                1824    1774    1681    1608    1455     985     525     360
                 101      46      21
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1347    4711    5906    6769    7056    8111    8671   10359 
   Size:       1347    3364    1195     863     287    1055     560    1688

Cuts at:  10359   10380 
   Size:         21

  Fragments arranged by size: 

                3364    1688    1347    1195    1055     863     560     287
                  21
 
BbvCI CC'TCA_GC

Cuts at:      0      61   10380 
   Size:         61   10319
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    4618    4652    8755    8789   10380 
   Size:       4618      34    4103      34    1591

  Fragments arranged by size: 

                4618    4103    1591      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1521    3360    4613    4899    5868    8620    9343    9709 
   Size:       1521    1839    1253     286     969    2752     723     366

Cuts at:   9709   10380 
   Size:        671

  Fragments arranged by size: 

                2752    1839    1521    1253     969     723     671     366
                 286
 
BclI T'GATC_A

Cuts at:      0    1046    2364    9077   10245   10380 
   Size:       1046    1318    6713    1168     135

  Fragments arranged by size: 

                6713    1318    1168    1046     135
 
BfrBI ATG'CAT

Cuts at:      0     705    7472   10380 
   Size:        705    6767    2908

  Fragments arranged by size: 

                6767    2908     705
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1027    4765   10380 
   Size:       1027    3738    5615

  Fragments arranged by size: 

                5615    3738    1027
 
BglII A'GATC_T

Cuts at:      0    1227    1832    5556    5626    7203    8273   10380 
   Size:       1227     605    3724      70    1577    1070    2107

  Fragments arranged by size: 

                3724    2107    1577    1227    1070     605      70
 
BlpI GC'TnA_GC

Cuts at:      0    4372   10380 
   Size:       4372    6008
 
Bme1580I G_kGCm'C

Cuts at:      0      21     121     454     566    2053    3100    4404    5305 
   Size:         21     100     333     112    1487    1047    1304     901

Cuts at:   5305    5894    5967    6278    6902    8560    9126    9713   10380 
   Size:        589      73     311     624    1658     566     587     667

  Fragments arranged by size: 

                1658    1487    1304    1047     901     667     624     589
                 587     566     333     311     112     100      73      21
            
 
BmgBI CAC'GTC

Cuts at:      0    6192   10380 
   Size:       6192    4188
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1090    2335    3679    4232    4755    6384    6703    7965 
   Size:       1090    1245    1344     553     523    1629     319    1262

Cuts at:   7965    8026    8865    9465    9791    9855   10380 
   Size:         61     839     600     326      64     525

  Fragments arranged by size: 

                1629    1344    1262    1245    1090     839     600     553
                 525     523     326     319      64      61
 
BmtI G_CTAG'C

Cuts at:      0    3505   10380 
   Size:       3505    6875
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4194   10380 
   Size:       4194    6186
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     701    1974    2293    2449    2869    3051    3571    3935 
   Size:        701    1273     319     156     420     182     520     364

Cuts at:   3935    4035    5035    6279    6853    9237   10201   10380 
   Size:        100    1000    1244     574    2384     964     179

  Fragments arranged by size: 

                2384    1273    1244    1000     964     701     574     520
                 420     364     319     182     179     156     100
 
Bpu10I CC'TnA_GC

Cuts at:      0      61    5316   10380 
   Size:         61    5255    5064

  Fragments arranged by size: 

                5255    5064      61
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2712    3038    9540   10380 
   Size:       2712     326    6502     840

  Fragments arranged by size: 

                6502    2712     840     326
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    4136    4948    6641    7525    9102    9844   10380 
   Size:       4136     812    1693     884    1577     742     536

  Fragments arranged by size: 

                4136    1693    1577     884     812     742     536
 
BsaAI yAC'GTr

Cuts at:      0     396    2351    7092    9672   10380 
   Size:        396    1955    4741    2580     708

  Fragments arranged by size: 

                4741    2580    1955     708     396
 
BsaBI GATnn'nnATC

Cuts at:      0    1226    3851    8825   10380 
   Size:       1226    2625    4974    1555

  Fragments arranged by size: 

                4974    2625    1555    1226
 
BsaHI Gr'CG_yC

Cuts at:      0     953    2262    3455    7639   10380 
   Size:        953    1309    1193    4184    2741

  Fragments arranged by size: 

                4184    2741    1309    1193     953
 
BsaWI w'CCGG_w

Cuts at:      0    1405    8490    9727   10380 
   Size:       1405    7085    1237     653

  Fragments arranged by size: 

                7085    1405    1237     653
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     604     634    1121    1151    1835    1865    3257    3287 
   Size:        604      30     487      30     684      30    1392      30

Cuts at:   3287    4865    4895    5196    5226    6722    6752    8035    8065 
   Size:       1578      30     301      30    1496      30    1283      30

Cuts at:   8065    9580    9610   10380 
   Size:       1515      30     770

  Fragments arranged by size: 

                1578    1515    1496    1392    1283     770     684     604
                 487     301      30      30      30      30      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1863    2151    2988    3463    6276    6412    9312    9588 
   Size:       1863     288     837     475    2813     136    2900     276

Cuts at:   9588   10380 
   Size:        792

  Fragments arranged by size: 

                2900    2813    1863     837     792     475     288     276
                 136
 
BseYI C'CCAG_C

Cuts at:      0      14      48     521     838    1025    2091    3233    3334 
   Size:         14      34     473     317     187    1066    1142     101

Cuts at:   3334    6223    8039    9163    9656   10124   10380 
   Size:       2889    1816    1124     493     468     256

  Fragments arranged by size: 

                2889    1816    1142    1124    1066     493     473     468
                 317     256     187     101      34      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     396    2373    3129    4289    5486    6274    6697    7473 
   Size:        396    1977     756    1160    1197     788     423     776

Cuts at:   7473    8347    8759    9705   10380 
   Size:        874     412     946     675

  Fragments arranged by size: 

                1977    1197    1160     946     874     788     776     756
                 675     423     412     396
 
BsiEI CG_ry'CG

Cuts at:      0    9728   10380 
   Size:       9728     652
 
BsiHKAI G_wGCw'C

Cuts at:      0     121     167     546     566    2053    2350    5317    6218 
   Size:        121      46     379      20    1487     297    2967     901

Cuts at:   6218    6278    6740    7575    8129    9117   10380 
   Size:         60     462     835     554     988    1263

  Fragments arranged by size: 

                2967    1487    1263     988     901     835     554     462
                 379     297     121      60      46      20
 
BsmI GAATG_Cn'

Cuts at:      0    1825    2902    3178    4752    6015    7264   10380 
   Size:       1825    1077     276    1574    1263    1249    3116

  Fragments arranged by size: 

                3116    1825    1574    1263    1249    1077     276
 
Bsp1286I G_dGCh'C

Cuts at:      0      21     121     167     454     546     566     906    2053 
   Size:         21     100      46     287      92      20     340    1147

Cuts at:   2053    2350    2361    2462    3100    4070    4404    5305    5317 
   Size:        297      11     101     638     970     334     901      12

Cuts at:   5317    5894    5967    6218    6278    6740    6902    7575    8129 
   Size:        577      73     251      60     462     162     673     554

Cuts at:   8129    8560    9117    9126    9713   10334   10380 
   Size:        431     557       9     587     621      46

  Fragments arranged by size: 

                1147     970     901     673     638     621     587     577
                 557     554     462     431     340     334     297     287
                 251     162     101     100      92      73      60      46
                  46      21      20      12      11       9
 
BspHI T'CATG_A

Cuts at:      0    1467    2454    2579    3972    6686    8202   10242   10380 
   Size:       1467     987     125    1393    2714    1516    2040     138

  Fragments arranged by size: 

                2714    2040    1516    1467    1393     987     138     125
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     413     453    3134    3506    4567    4697    4719    6809 
   Size:        413      40    2681     372    1061     130      22    2090

Cuts at:   6809    7829   10380 
   Size:       1020    2551

  Fragments arranged by size: 

                2681    2551    2090    1061    1020     413     372     130
                  40      22
 
BsrDI GCAATG_nn'

Cuts at:      0     717    1261    3833    6816    6824    9090   10380 
   Size:        717     544    2572    2983       8    2266    1290

  Fragments arranged by size: 

                2983    2572    2266    1290     717     544       8
 
BsrFI r'CCGG_y

Cuts at:      0    1405    6340    8065    9727   10380 
   Size:       1405    4935    1725    1662     653

  Fragments arranged by size: 

                4935    1725    1662    1405     653
 
BsrGI T'GTAC_A

Cuts at:      0    4217    5043    5566    8149    9674   10380 
   Size:       4217     826     523    2583    1525     706

  Fragments arranged by size: 

                4217    2583    1525     826     706     523
 
BssHII G'CGCG_C

Cuts at:      0      67      69   10380 
   Size:         67       2   10311

  Fragments arranged by size: 

               10311      67       2
 
BssSI C'ACGA_G

Cuts at:      0     743    6644    6768    8044   10380 
   Size:        743    5901     124    1276    2336

  Fragments arranged by size: 

                5901    2336    1276     743     124
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3173    4037    4559    6159    9503   10380 
   Size:       3173     864     522    1600    3344     877

  Fragments arranged by size: 

                3344    3173    1600     877     864     522
 
BstBI TT'CG_AA

Cuts at:      0    6518   10380 
   Size:       6518    3862
 
BstEII G'GTnAC_C

Cuts at:      0     361    9400   10380 
   Size:        361    9039     980

  Fragments arranged by size: 

                9039     980     361
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     358     653    1731    2628    6359    6813    7663    9171 
   Size:        358     295    1078     897    3731     454     850    1508

Cuts at:   9171    9963   10380 
   Size:        792     417

  Fragments arranged by size: 

                3731    1508    1078     897     850     792     454     417
                 358     295
 
BstYI r'GATC_y

Cuts at:      0    1227    1832    4163    5556    5626    6108    6301    7203 
   Size:       1227     605    2331    1393      70     482     193     902

Cuts at:   7203    7283    7695    8273    8791    8826    9623    9966   10380 
   Size:         80     412     578     518      35     797     343     414

  Fragments arranged by size: 

                2331    1393    1227     902     797     605     578     518
                 482     414     412     343     193      80      70      35
            
 
Bsu36I CC'TnA_GG

Cuts at:      0     947    1420    5295   10380 
   Size:        947     473    3875    5085

  Fragments arranged by size: 

                5085    3875     947     473
 
BtgI C'CryG_G

Cuts at:      0     126    1179    1394    1788    2187    2865    4254    6104 
   Size:        126    1053     215     394     399     678    1389    1850

Cuts at:   6104    7461    7634    8586    9906   10380 
   Size:       1357     173     952    1320     474

  Fragments arranged by size: 

                1850    1389    1357    1320    1053     952     678     474
                 399     394     215     173     126
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0   10104   10380 
   Size:      10104     276
 
BtsI GCAGTG_nn'

Cuts at:      0      15     384    3100    3117    4254    5744    7461    8231 
   Size:         15     369    2716      17    1137    1490    1717     770

Cuts at:   8231    8536    8771   10079   10380 
   Size:        305     235    1308     301

  Fragments arranged by size: 

                2716    1717    1490    1308    1137     770     369     305
                 301     235      17      15
 
ClaI AT'CG_AT

Cuts at:      0    3855    5837    6923    8540   10380 
   Size:       3855    1982    1086    1617    1840

  Fragments arranged by size: 

                3855    1982    1840    1617    1086
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     961     996    3043    3078   10380 
   Size:        961      35    2047      35    7302

  Fragments arranged by size: 

                7302    2047     961      35      35
 
DraI TTT'AAA

Cuts at:      0    5523    9366   10380 
   Size:       5523    3843    1014

  Fragments arranged by size: 

                5523    3843    1014
 
DraIII CAC_nnn'GTG

Cuts at:      0    8750   10062   10380 
   Size:       8750    1312     318

  Fragments arranged by size: 

                8750    1312     318
 
EaeI y'GGCC_r

Cuts at:      0    2222    2682    2808    4251    7458    9283   10380 
   Size:       2222     460     126    1443    3207    1825    1097

  Fragments arranged by size: 

                3207    2222    1825    1443    1097     460     126
 
EarI CTCTTCn'nnn_

Cuts at:      0    3309    5090    7544    9492   10380 
   Size:       3309    1781    2454    1948     888

  Fragments arranged by size: 

                3309    2454    1948    1781     888
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3425   10380 
   Size:       3425    6955
 
EcoICRI GAG'CTC

Cuts at:      0     544    7573   10380 
   Size:        544    7029    2807

  Fragments arranged by size: 

                7029    2807     544
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     701    1576    1974    2216    2293    2449    2869    3051 
   Size:        701     875     398     242      77     156     420     182

Cuts at:   3051    3309    3448    3510    3571    3935    3946    4035    4532 
   Size:        258     139      62      61     364      11      89     497

Cuts at:   4532    5010    5035    5474    5990    6279    6766    6853    8004 
   Size:        478      25     439     516     289     487      87    1151

Cuts at:   8004    8099    8270    8683    8695    9047    9237   10201   10380 
   Size:         95     171     413      12     352     190     964     179

  Fragments arranged by size: 

                1151     964     875     701     516     497     487     478
                 439     420     413     398     364     352     289     258
                 242     190     182     179     171     156     139      95
                  89      87      77      62      61      25      12      11
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     946    2375    4693   10380 
   Size:        946    1429    2318    5687

  Fragments arranged by size: 

                5687    2318    1429     946
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2235    3360    5219    7349    8557   10380 
   Size:       2235    1125    1859    2130    1208    1823

  Fragments arranged by size: 

                2235    2130    1859    1823    1208    1125
 
EcoRI G'AATT_C

Cuts at:      0    2507    8756   10380 
   Size:       2507    6249    1624

  Fragments arranged by size: 

                6249    2507    1624
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2917    3208    4562    8700   10380 
   Size:       2917     291    1354    4138    1680

  Fragments arranged by size: 

                4138    2917    1680    1354     291
 
FspI TGC'GCA

Cuts at:      0    6631   10380 
   Size:       6631    3749
 
HaeII r_GCGC'y

Cuts at:      0      43    1797    8030    8052   10380 
   Size:         43    1754    6233      22    2328

  Fragments arranged by size: 

                6233    2328    1754      43      22
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     323     355     604     636    1150    1182    1243    1275 
   Size:        323      32     249      32     514      32      61      32

Cuts at:   1275    1280    1312    1835    1867    2092    2124    4426    4458 
   Size:          5      32     523      32     225      32    2302      32

Cuts at:   4458    4473    4505    4639    4671    4882    4914    5463    5495 
   Size:         15      32     134      32     211      32     549      32

Cuts at:   5495    5759    5791    6085    6117    7670    7702    7890    7922 
   Size:        264      32     294      32    1553      32     188      32

Cuts at:   7922    8643    8675    9140    9172    9427    9459    9580    9612 
   Size:        721      32     465      32     255      32     121      32

Cuts at:   9612    9757    9789   10380 
   Size:        145      32     591

  Fragments arranged by size: 

                2302    1553     721     591     549     523     514     465
                 323     294     264     255     249     225     211     188
                 145     134     121      61      32      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      32      32      32
                  32      15       5
 
HincII GTy'rAC

Cuts at:      0    6730    9549   10095   10380 
   Size:       6730    2819     546     285

  Fragments arranged by size: 

                6730    2819     546     285
 
HindIII A'AGCT_T

Cuts at:      0    2139    8475   10380 
   Size:       2139    6336    1905

  Fragments arranged by size: 

                6336    2139    1905
 
KpnI G_GTAC'C

Cuts at:      0    3162    6324   10380 
   Size:       3162    3162    4056

  Fragments arranged by size: 

                4056    3162    3162
 
MfeI C'AATT_G

Cuts at:      0    1991    5904    9169    9662   10380 
   Size:       1991    3913    3265     493     718

  Fragments arranged by size: 

                3913    3265    1991     718     493
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     571    3721    4389    4625    5134    6493    7567    8262 
   Size:        571    3150     668     236     509    1359    1074     695

Cuts at:   8262    8335    8458    8909   10380 
   Size:         73     123     451    1471

  Fragments arranged by size: 

                3150    1471    1359    1074     695     668     571     509
                 451     236     123      73
 
MscI TGG'CCA

Cuts at:      0    2224    2684    2810    4253    7460    9285   10380 
   Size:       2224     460     126    1443    3207    1825    1095

  Fragments arranged by size: 

                3207    2224    1825    1443    1095     460     126
 
MslI CAynn'nnrTG

Cuts at:      0     710     847    1184    1472    2954    3205    4725    6296 
   Size:        710     137     337     288    1482     251    1520    1571

Cuts at:   6296    6637    7027    7447    7661    8098   10380 
   Size:        341     390     420     214     437    2282

  Fragments arranged by size: 

                2282    1571    1520    1482     710     437     420     390
                 341     337     288     251     214     137
 
MspA1I CmG'CkG

Cuts at:      0    3029    3348    3562    3593    6106    7916    9582    9620 
   Size:       3029     319     214      31    2513    1810    1666      38

Cuts at:   9620    9707    9891   10031   10380 
   Size:         87     184     140     349

  Fragments arranged by size: 

                3029    2513    1810    1666     349     319     214     184
                 140      87      38      31
 
NaeI GCC'GGC

Cuts at:      0    6342    8067   10380 
   Size:       6342    1725    2313

  Fragments arranged by size: 

                6342    2313    1725
 
NcoI C'CATG_G

Cuts at:      0    1179    1394    1788    4254    7461    8586    9906   10380 
   Size:       1179     215     394    2466    3207    1125    1320     474

  Fragments arranged by size: 

                3207    2466    1320    1179    1125     474     394     215
            
 
NdeI CA'TA_TG

Cuts at:      0     735    3139   10380 
   Size:        735    2404    7241

  Fragments arranged by size: 

                7241    2404     735
 
NgoMIV G'CCGG_C

Cuts at:      0    6340    8065   10380 
   Size:       6340    1725    2315

  Fragments arranged by size: 

                6340    2315    1725
 
NheI G'CTAG_C

Cuts at:      0    3501   10380 
   Size:       3501    6879
 
NsiI A_TGCA'T

Cuts at:      0     707    7474   10380 
   Size:        707    6767    2906

  Fragments arranged by size: 

                6767    2906     707
 
NspI r_CATG'y

Cuts at:      0    1986    3108    6636    6667    7452    8097    8529   10017 
   Size:       1986    1122    3528      31     785     645     432    1488

Cuts at:  10017   10117   10380 
   Size:        100     263

  Fragments arranged by size: 

                3528    1986    1488    1122     785     645     432     263
                 100      31
 
PasI CC'CwG_GG

Cuts at:      0    4462    6914   10380 
   Size:       4462    2452    3466

  Fragments arranged by size: 

                4462    3466    2452
 
PciI A'CATG_T

Cuts at:      0    1982    3104    7448    8093   10380 
   Size:       1982    1122    4344     645    2287

  Fragments arranged by size: 

                4344    2287    1982    1122     645
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1975    2680    5116    8976    9174   10062   10380 
   Size:       1975     705    2436    3860     198     888     318

  Fragments arranged by size: 

                3860    2436    1975     888     705     318     198
 
PfoI T'CCnGG_A

Cuts at:      0     207    2426    4166    4661    8622   10380 
   Size:        207    2219    1740     495    3961    1758

  Fragments arranged by size: 

                3961    2219    1758    1740     495     207
 
PmlI CAC'GTG

Cuts at:      0     396    2351    9672   10380 
   Size:        396    1955    7321     708

  Fragments arranged by size: 

                7321    1955     708     396
 
PpuMI rG'GwC_Cy

Cuts at:      0    3360    5219    7349   10380 
   Size:       3360    1859    2130    3031

  Fragments arranged by size: 

                3360    3031    2130    1859
 
PshAI GACnn'nnGTC

Cuts at:      0   10359   10380 
   Size:      10359      21
 
PsiI TTA'TAA

Cuts at:      0    5789   10380 
   Size:       5789    4591
 
PspOMI G'GGCC_C

Cuts at:      0      17    5890    8556   10380 
   Size:         17    5873    2666    1824

  Fragments arranged by size: 

                5873    2666    1824      17
 
PspXI vC'TCGA_Gb

Cuts at:      0    9974   10380 
   Size:       9974     406
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    9323    9355   10380 
   Size:       9323      32    1025

  Fragments arranged by size: 

                9323    1025      32
 
PstI C_TGCA'G

Cuts at:      0    4029    4249    8061    8581    9706   10380 
   Size:       4029     220    3812     520    1125     674

  Fragments arranged by size: 

                4029    3812    1125     674     520     220
 
PvuII CAG'CTG

Cuts at:      0    3348    3562    9582    9891   10031   10380 
   Size:       3348     214    6020     309     140     349

  Fragments arranged by size: 

                6020    3348     349     309     214     140
 
SacI G_AGCT'C

Cuts at:      0     546    7575   10380 
   Size:        546    7029    2805

  Fragments arranged by size: 

                7029    2805     546
 
SacII CC_GC'GG

Cuts at:      0    6107   10380 
   Size:       6107    4273
 
ScaI AGT'ACT

Cuts at:      0    2854    3945    3967    8518    8739   10380 
   Size:       2854    1091      22    4551     221    1641

  Fragments arranged by size: 

                4551    2854    1641    1091     221      22
 
SexAI A'CCwGG_T

Cuts at:      0    2484    2837    3583    7345   10380 
   Size:       2484     353     746    3762    3035

  Fragments arranged by size: 

                3762    3035    2484     746     353
 
SfcI C'TryA_G

Cuts at:      0     476    1133    1283    1661    1884    3901    4025    4245 
   Size:        476     657     150     378     223    2017     124     220

Cuts at:   4245    4838    5013    6742    6906    7132    8057    8577    9702 
   Size:        593     175    1729     164     226     925     520    1125

Cuts at:   9702   10380 
   Size:        678

  Fragments arranged by size: 

                2017    1729    1125     925     678     657     593     520
                 476     378     226     223     220     175     164     150
                 124
 
SmlI C'TyrA_G

Cuts at:      0    2727    3053    3869    8598    9555    9974   10380 
   Size:       2727     326     816    4729     957     419     406

  Fragments arranged by size: 

                4729    2727     957     816     419     406     326
 
SspI AAT'ATT

Cuts at:      0    6948    7792   10380 
   Size:       6948     844    2588

  Fragments arranged by size: 

                6948    2588     844
 
StuI AGG'CCT

Cuts at:      0     764   10380 
   Size:        764    9616
 
StyI C'CwwG_G

Cuts at:      0    1179    1394    1788    1974    2658    3162    4126    4254 
   Size:       1179     215     394     186     684     504     964     128

Cuts at:   4254    4869    5305    7461    7773    8586    9759    9906   10380 
   Size:        615     436    2156     312     813    1173     147     474

  Fragments arranged by size: 

                2156    1179    1173     964     813     684     615     504
                 474     436     394     312     215     186     147     128
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     136     581    1880    3194    3366    5923    6373    7028 
   Size:        136     445    1299    1314     172    2557     450     655

Cuts at:   7028    7238    7733   10380 
   Size:        210     495    2647

  Fragments arranged by size: 

                2647    2557    1314    1299     655     495     450     445
                 210     172     136
 
TatI w'GTAC_w

Cuts at:      0    2852    3320    3467    3512    3943    3965    4217    5043 
   Size:       2852     468     147      45     431      22     252     826

Cuts at:   5043    5286    5566    7514    7618    7847    8149    8516    8737 
   Size:        243     280    1948     104     229     302     367     221

Cuts at:   8737    9674    9920   10380 
   Size:        937     246     460

  Fragments arranged by size: 

                2852    1948     937     826     468     460     431     367
                 302     280     252     246     243     229     221     147
                 104      45      22
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4304    4336    5196    5228    5333    5365    6477    6509 
   Size:       4304      32     860      32     105      32    1112      32

Cuts at:   6509    8035    8067    8201    8233    8276    8308    8783    8815 
   Size:       1526      32     134      32      43      32     475      32

Cuts at:   8815   10380 
   Size:       1565

  Fragments arranged by size: 

                4304    1565    1526    1112     860     475     134     105
                  43      32      32      32      32      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3385    7969    9592   10380 
   Size:       3385    4584    1623     788

  Fragments arranged by size: 

                4584    3385    1623     788
 
XbaI T'CTAG_A

Cuts at:      0    5559   10380 
   Size:       5559    4821
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     799    4411    5250    8441    9171    9676   10380 
   Size:        799    3612     839    3191     730     505     704

  Fragments arranged by size: 

                3612    3191     839     799     730     704     505
 
XhoI C'TCGA_G

Cuts at:      0    3869    9974   10380 
   Size:       3869    6105     406

  Fragments arranged by size: 

                6105    3869     406
 
XmnI GAAnn'nnTTC

Cuts at:      0     995    1244    2900    6588    6948    8409    8668   10380 
   Size:        995     249    1656    3688     360    1461     259    1712

  Fragments arranged by size: 

                3688    1712    1656    1461     995     360     259     249
            
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     KpnI     MfeI     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI
     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI    PvuII     SacI
    SacII     ScaI    SexAI     SfcI     SmlI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AloI     AscI    AsiSI    AvrII     BaeI     BbeI    BsiWI
    BsmBI    BspEI    BsrBI  BstZ17I     DrdI     EagI    EcoRV     FseI
    FspAI     HpaI     KasI     MluI     NarI     NotI     NruI     PacI
     PmeI     PpiI     PvuI    RsrII     SalI    SanDI     SapI     SbfI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SwaI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com