Restriction map for pF1KE1780
 (Linear) (Six-base) MAPSORT of: KIEE1780.seq  Check: 5262  from: 1  to: 864



KIEE1780.orf

 With 182 enzymes: SgfI * 

                        June 10, 2016 16:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     166     864 
   Size:        166     698
 
Acc65I G'GTAC_C

Cuts at:      0     200     864 
   Size:        200     664
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     646     864 
   Size:        646     218
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     559     864 
   Size:        559     305
 
AlwNI CAG_nnn'CTG

Cuts at:      0      92     624     828     864 
   Size:         92     532     204      36

  Fragments arranged by size: 

                 532     204      92      36
 
ApaI G_GGCC'C

Cuts at:      0     339     864 
   Size:        339     525
 
AvaI C'yCGr_G

Cuts at:      0     247     578     864 
   Size:        247     331     286

  Fragments arranged by size: 

                 331     286     247
 
BanI G'GyrC_C

Cuts at:      0      15     200     355     772     864 
   Size:         15     185     155     417      92

  Fragments arranged by size: 

                 417     185     155      92      15
 
BanII G_rGCy'C

Cuts at:      0     214     339     425     710     799     864 
   Size:        214     125      86     285      89      65

  Fragments arranged by size: 

                 285     214     125      89      86      65
 
BbeI G_GCGC'C

Cuts at:      0      19     864 
   Size:         19     845
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     703     864 
   Size:        703     161
 
BbvCI CC'TCA_GC

Cuts at:      0     639     864 
   Size:        639     225
 
BglI GCCn_nnn'nGGC

Cuts at:      0     266     306     391     533     831     864 
   Size:        266      40      85     142     298      33

  Fragments arranged by size: 

                 298     266     142      85      40      33
 
BlpI GC'TnA_GC

Cuts at:      0     798     864 
   Size:        798      66
 
Bme1580I G_kGCm'C

Cuts at:      0     339     455     719     775     822     864 
   Size:        339     116     264      56      47      42

  Fragments arranged by size: 

                 339     264     116      56      47      42
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      58     864 
   Size:         58     806
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     654     864 
   Size:        654     210
 
Bpu10I CC'TnA_GC

Cuts at:      0     424     639     728     864 
   Size:        424     215      89     136

  Fragments arranged by size: 

                 424     215     136      89
 
BsaHI Gr'CG_yC

Cuts at:      0      16     864 
   Size:         16     848
 
BseYI C'CCAG_C

Cuts at:      0     114     214     466     531     821     864 
   Size:        114     100     252      65     290      43

  Fragments arranged by size: 

                 290     252     114     100      65      43
 
BsiHKAI G_wGCw'C

Cuts at:      0     425     864 
   Size:        425     439
 
Bsp1286I G_dGCh'C

Cuts at:      0     214     339     425     455     710     719     775     799 
   Size:        214     125      86      30     255       9      56      24

Cuts at:    799     822     864 
   Size:         23      42

  Fragments arranged by size: 

                 255     214     125      86      56      42      30      24
                  23       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     166     864 
   Size:        166     698
 
BsrDI GCAATG_nn'

Cuts at:      0     351     864 
   Size:        351     513
 
BsrFI r'CCGG_y

Cuts at:      0     487     613     864 
   Size:        487     126     251

  Fragments arranged by size: 

                 487     251     126
 
BssSI C'ACGA_G

Cuts at:      0     124     587     864 
   Size:        124     463     277

  Fragments arranged by size: 

                 463     277     124
 
BstEII G'GTnAC_C

Cuts at:      0     129     864 
   Size:        129     735
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      32     381     864 
   Size:         32     349     483

  Fragments arranged by size: 

                 483     349      32
 
BstYI r'GATC_y

Cuts at:      0       5     864 
   Size:          5     859
 
Bsu36I CC'TnA_GG

Cuts at:      0     836     864 
   Size:        836      28
 
BtsI GCAGTG_nn'

Cuts at:      0     846     864 
   Size:        846      18
 
EaeI y'GGCC_r

Cuts at:      0      22      58     236     267     489     563     615     864 
   Size:         22      36     178      31     222      74      52     249

  Fragments arranged by size: 

                 249     222     178      74      52      36      31      22
            
 
EcoICRI GAG'CTC

Cuts at:      0     423     864 
   Size:        423     441
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     646     864 
   Size:        646     218
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     477     628     864 
   Size:        477     151     236

  Fragments arranged by size: 

                 477     236     151
 
EcoO109I rG'GnC_Cy

Cuts at:      0      89     335     558     633     864 
   Size:         89     246     223      75     231

  Fragments arranged by size: 

                 246     231     223      89      75
 
HaeII r_GCGC'y

Cuts at:      0      19     864 
   Size:         19     845
 
KasI G'GCGC_C

Cuts at:      0      15     864 
   Size:         15     849
 
KpnI G_GTAC'C

Cuts at:      0     204     864 
   Size:        204     660
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     194     864 
   Size:        194     670
 
MscI TGG'CCA

Cuts at:      0      24     864 
   Size:         24     840
 
MslI CAynn'nnrTG

Cuts at:      0     325     864 
   Size:        325     539
 
MspA1I CmG'CkG

Cuts at:      0     353     802     864 
   Size:        353     449      62

  Fragments arranged by size: 

                 449     353      62
 
NaeI GCC'GGC

Cuts at:      0     489     864 
   Size:        489     375
 
NarI GG'CG_CC

Cuts at:      0      16     864 
   Size:         16     848
 
NgoMIV G'CCGG_C

Cuts at:      0     487     864 
   Size:        487     377
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     504     864 
   Size:        504     360
 
PpuMI rG'GwC_Cy

Cuts at:      0     558     633     864 
   Size:        558      75     231

  Fragments arranged by size: 

                 558     231      75
 
PspOMI G'GGCC_C

Cuts at:      0     335     864 
   Size:        335     529
 
PstI C_TGCA'G

Cuts at:      0     163     864 
   Size:        163     701
 
PvuII CAG'CTG

Cuts at:      0     802     864 
   Size:        802      62
 
SacI G_AGCT'C

Cuts at:      0     425     864 
   Size:        425     439
 
SfcI C'TryA_G

Cuts at:      0     159     864 
   Size:        159     705
 
SfoI GGC'GCC

Cuts at:      0      17     864 
   Size:         17     847
 
SmaI CCC'GGG

Cuts at:      0     580     864 
   Size:        580     284
 
StuI AGG'CCT

Cuts at:      0     834     864 
   Size:        834      30
 
StyI C'CwwG_G

Cuts at:      0     389     864 
   Size:        389     475
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     148     492     864 
   Size:        148     344     372

  Fragments arranged by size: 

                 372     344     148
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     670     702     864 
   Size:        670      32     162

  Fragments arranged by size: 

                 670     162      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      17      89     864 
   Size:         17      72     775

  Fragments arranged by size: 

                 775      72      17
 
XmaI C'CCGG_G

Cuts at:      0     578     864 
   Size:        578     286
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AlfI    AlwNI     ApaI     AvaI     BanI
    BanII     BbeI     BbsI    BbvCI     BglI     BlpI Bme1580I     BmrI
     BplI   Bpu10I    BsaHI    BseYI  BsiHKAI Bsp1286I    BspMI    BsrDI
    BsrFI    BssSI   BstEII    BstXI    BstYI   Bsu36I     BtsI     EaeI
  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII     KasI     KpnI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV    PflMI    PpuMI
   PspOMI     PstI    PvuII     SacI     SfcI     SfoI     SmaI     StuI
     StyI    TaqII     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrGI   BssHII   BstAPI    BstBI  BstZ17I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     MfeI     MluI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI  Tth111I     XbaI     XhoI     XmnI     ZraI
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