Restriction map for pF1KB8272
 (Circular) (Six-base) MAPSORT of: pF1KB8272.seq  Check: 4225  from: 1  to: 3596



pF1KB8272  3596 bp

 With 182 enzymes: SgfI * 

                        June 2, 2011 17:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    470     470 
   Size:       3596
 
AccI GT'mk_AC

Cuts at:    634     634 
   Size:       3596
 
Acc65I G'GTAC_C

Cuts at:    612     612 
   Size:       3596
 
AclI AA'CG_TT

Cuts at:   3436    3436 
   Size:       3596
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    208    1421    1853    2600    2868     208 
   Size:       1213     432     747     268     936

  Fragments arranged by size: 

                1213     936     747     432     268
 
AflIII A'CryG_T

Cuts at:    113     948    2052    3029     113 
   Size:        835    1104     977     680

  Fragments arranged by size: 

                1104     977     835     680
 
AgeI A'CCGG_T

Cuts at:   1984    1984 
   Size:       3596
 
AlwNI CAG_nnn'CTG

Cuts at:    237     901    2468     237 
   Size:        664    1567    1365

  Fragments arranged by size: 

                1567    1365     664
 
ApaLI G'TGCA_C

Cuts at:    457    2366     457 
   Size:       1909    1687
 
ApoI r'AATT_y

Cuts at:    600    2949    3205     600 
   Size:       2349     256     991

  Fragments arranged by size: 

                2349     991     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3596
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3596
 
AvaI C'yCGr_G

Cuts at:    429     616     429 
   Size:        187    3409
 
BamHI G'GATC_C

Cuts at:    621     621 
   Size:       3596
 
BanI G'GyrC_C

Cuts at:    131     612    1026    1274    1309     131 
   Size:        481     414     248      35    2418

  Fragments arranged by size: 

                2418     481     414     248      35
 
BanII G_rGCy'C

Cuts at:    610    1640     610 
   Size:       1030    2566
 
BbeI G_GCGC'C

Cuts at:    135    1030    1278     135 
   Size:        895     248    2453

  Fragments arranged by size: 

                2453     895     248
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    162     196     162 
   Size:         34    3562
 
BciVI GTATCCnnnnn_n'

Cuts at:   1494    2255    3596    1494 
   Size:        761    1341    1494

  Fragments arranged by size: 

                1494    1341     761
 
BclI T'GATC_A

Cuts at:   1116    1116 
   Size:       3596
 
BglI GCCn_nnn'nGGC

Cuts at:    346     346 
   Size:       3596
 
BglII A'GATC_T

Cuts at:   1111    3148    1111 
   Size:       2037    1559
 
BlpI GC'TnA_GC

Cuts at:    708     708 
   Size:       3596
 
Bme1580I G_kGCm'C

Cuts at:    461    1221    1314    2370     461 
   Size:        760      93    1056    1687

  Fragments arranged by size: 

                1687    1056     760      93
 
BmgBI CAC'GTC

Cuts at:    114     114 
   Size:       3596
 
BmrI ACTGGGnnnn_n'

Cuts at:    286     808     889    1224    3368     286 
   Size:        522      81     335    2144     514

  Fragments arranged by size: 

                2144     522     514     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1963    1963 
   Size:       3596
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    528     528 
   Size:       3596
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    196     772    1651    2143    2441     196 
   Size:        576     879     492     298    1351

  Fragments arranged by size: 

                1351     879     576     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3300    3300 
   Size:       3596
 
BsaAI yAC'GTr

Cuts at:    949     949 
   Size:       3596
 
BsaBI GATnn'nnATC

Cuts at:      6    1110    3147       6 
   Size:       1104    2037     455

  Fragments arranged by size: 

                2037    1104     455
 
BsaHI Gr'CG_yC

Cuts at:    132     158    1027    1275    1977    3473     132 
   Size:         26     869     248     702    1496     255

  Fragments arranged by size: 

                1496     869     702     255     248      26
 
BsaWI w'CCGG_w

Cuts at:    785    1306    1984    2258    2405    2816     785 
   Size:        521     678     274     147     411    1565

  Fragments arranged by size: 

                1565     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    501     531     501 
   Size:         30    3566
 
BseYI C'CCAG_C

Cuts at:    321     581    1021    1779    2356    2852     321 
   Size:        260     440     758     577     496    1065

  Fragments arranged by size: 

                1065     758     577     496     440     260
 
BsiEI CG_ry'CG

Cuts at:    104    1138    1184    2392     104 
   Size:       1034      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1034      46
 
BsiHKAI G_wGCw'C

Cuts at:    461     610    1388    1578    2370     461 
   Size:        149     778     190     792    1687

  Fragments arranged by size: 

                1687     792     778     190     149
 
Bsp1286I G_dGCh'C

Cuts at:    461     610    1221    1314    1388    1578    1640    2370     461 
   Size:        149     611      93      74     190      62     730    1687

  Fragments arranged by size: 

                1687     730     611     190     149      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2816    2816 
   Size:       3596
 
BspHI T'CATG_A

Cuts at:    122    2772     122 
   Size:       2650     946
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    470     646     860    1162    1543     470 
   Size:        176     214     302     381    2523

  Fragments arranged by size: 

                2523     381     302     214     176
 
BsrBI CCG'CTC

Cuts at:     50    1888    1942    3426    3591      50 
   Size:       1838      54    1484     165      55

  Fragments arranged by size: 

                1838    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1508    1508 
   Size:       3596
 
BsrFI r'CCGG_y

Cuts at:   1594    1775    1984    1594 
   Size:        181     209    3206

  Fragments arranged by size: 

                3206     209     181
 
BsrGI T'GTAC_A

Cuts at:    239     239 
   Size:       3596
 
BssSI C'ACGA_G

Cuts at:   1867    2225    1867 
   Size:        358    3238
 
BstAPI GCAn_nnn'nTGC

Cuts at:    973     973 
   Size:       3596
 
BstBI TT'CG_AA

Cuts at:    182    1957     182 
   Size:       1775    1821
 
BstXI CCAn_nnnn'nTGG

Cuts at:    520     520 
   Size:       3596
 
BstYI r'GATC_y

Cuts at:      1     621    1088    1111    1446    1692    2704    2790    2802 
   Size:        620     467      23     335     246    1012      86      12

Cuts at:   2802    3148       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     620     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3596
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1546    1727    1890    1546 
   Size:        181     163    3252

  Fragments arranged by size: 

                3252     181     163
 
BtsI GCAGTG_nn'

Cuts at:    444     839     444 
   Size:        395    3201
 
DraI TTT'AAA

Cuts at:    595     595 
   Size:       3596
 
DrdI GACnn_nn'nnGTC

Cuts at:   1302    2160    1302 
   Size:        858    2738
 
EaeI y'GGCC_r

Cuts at:    310     485    1181    1355    1746    1773    3118     310 
   Size:        175     696     174     391      27    1345     788

  Fragments arranged by size: 

                1345     788     696     391     175     174      27
 
EagI C'GGCC_G

Cuts at:   1181    1181 
   Size:       3596
 
EarI CTCTTCn'nnn_

Cuts at:   1619    1829    1619 
   Size:        210    3386
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1804    2114    2260    3406    1804 
   Size:        310     146    1146    1994

  Fragments arranged by size: 

                1994    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    608     608 
   Size:       3596
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    208     528    1421    1853    2600    2868     208 
   Size:        320     893     432     747     268     936

  Fragments arranged by size: 

                 936     893     747     432     320     268
 
EcoO109I rG'GnC_Cy

Cuts at:    318     366     735     318 
   Size:         48     369    3179

  Fragments arranged by size: 

                3179     369      48
 
EcoRI G'AATT_C

Cuts at:    600     600 
   Size:       3596
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1615    1615 
   Size:       3596
 
FspI TGC'GCA

Cuts at:   1377    1377 
   Size:       3596
 
HaeII r_GCGC'y

Cuts at:    135    1030    1278    2300    3279     135 
   Size:        895     248    1022     979     452

  Fragments arranged by size: 

                1022     979     895     452     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    501     533    1438    1468    1470    1500     501 
   Size:         32     905      30       2      30    2597

  Fragments arranged by size: 

                2597     905      32      30      30       2
 
HincII GTy'rAC

Cuts at:    635     635 
   Size:       3596
 
KasI G'GCGC_C

Cuts at:    131    1026    1274     131 
   Size:        895     248    2453

  Fragments arranged by size: 

                2453     895     248
 
KpnI G_GTAC'C

Cuts at:    616     616 
   Size:       3596
 
MluI A'CGCG_T

Cuts at:   3029    3029 
   Size:       3596
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2267    2451    2267 
   Size:        184    3412
 
MscI TGG'CCA

Cuts at:    487    1357     487 
   Size:        870    2726
 
MslI CAynn'nnrTG

Cuts at:    112     464    1585    1712     112 
   Size:        352    1121     127    1996

  Fragments arranged by size: 

                1996    1121     352     127
 
MspA1I CmG'CkG

Cuts at:    234     707    1021    1381    2394    2639     234 
   Size:        473     314     360    1013     245    1191

  Fragments arranged by size: 

                1191    1013     473     360     314     245
 
NaeI GCC'GGC

Cuts at:   1777    1777 
   Size:       3596
 
NarI GG'CG_CC

Cuts at:    132    1027    1275     132 
   Size:        895     248    2453

  Fragments arranged by size: 

                2453     895     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3596
 
NgoMIV G'CCGG_C

Cuts at:   1775    1775 
   Size:       3596
 
NspI r_CATG'y

Cuts at:    469     536     649    1149    2056     469 
   Size:         67     113     500     907    2009

  Fragments arranged by size: 

                2009     907     500     113      67
 
PciI A'CATG_T

Cuts at:   2052    2052 
   Size:       3596
 
PflMI CCAn_nnn'nTGG

Cuts at:    258     483     258 
   Size:        225    3371
 
PmeI GTTT'AAAC

Cuts at:    595     595 
   Size:       3596
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2735    2767    2735 
   Size:         32    3564
 
PstI C_TGCA'G

Cuts at:    643    1328     643 
   Size:        685    2911
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3596
 
PvuII CAG'CTG

Cuts at:   1021    1381    1021 
   Size:        360    3236
 
RsrII CG'GwC_CG

Cuts at:   1791    1791 
   Size:       3596
 
SacI G_AGCT'C

Cuts at:    610     610 
   Size:       3596
 
SalI G'TCGA_C

Cuts at:    633     633 
   Size:       3596
 
SapI GCTCTTCn'nnn_

Cuts at:   1619    1829    1619 
   Size:        210    3386
 
SbfI CC_TGCA'GG

Cuts at:    643     643 
   Size:       3596
 
ScaI AGT'ACT

Cuts at:   3113    3113 
   Size:       3596
 
SfcI C'TryA_G

Cuts at:     33     639    1324    2317    2508      33 
   Size:        606     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     606     191
 
SfoI GGC'GCC

Cuts at:    133    1028    1276     133 
   Size:        895     248    2453

  Fragments arranged by size: 

                2453     895     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3596
 
SmaI CCC'GGG

Cuts at:    618     618 
   Size:       3596
 
SmlI C'TyrA_G

Cuts at:    211     751    1666    2158    2420     211 
   Size:        540     915     492     262    1387

  Fragments arranged by size: 

                1387     915     540     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3596
 
SphI G_CATG'C

Cuts at:    649    1149     649 
   Size:        500    3096
 
StyI C'CwwG_G

Cuts at:    107     161     730    1084     107 
   Size:         54     569     354    2619

  Fragments arranged by size: 

                2619     569     354      54
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    590    1179    1312    1771    1980     590 
   Size:        589     133     459     209    2206

  Fragments arranged by size: 

                2206     589     459     209     133
 
TatI w'GTAC_w

Cuts at:    239    3111     239 
   Size:       2872     724
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3113    3145    3113 
   Size:         32    3564
 
Tth111I GACn'n_nGTC

Cuts at:   1393    1393 
   Size:       3596
 
XbaI T'CTAG_A

Cuts at:    627     627 
   Size:       3596
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    333    1091     333 
   Size:        758    2838
 
XmaI C'CCGG_G

Cuts at:    616     616 
   Size:       3596
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PciI    PflMI     PmeI     PpiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI    BfrBI     BmtI   Bpu10I
    BseRI     BsgI    BsiWI     BsmI    BsmBI   BssHII   BstEII  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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