Restriction map for pF1KE2133
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        AlwNI       
AocI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbvCI       BfrI        BglI        BlnI        
BlpI        Bpu1102I    BseAI       BsiWI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrGI       
Bst1107I    BstBI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoT22I     
FauI        FseI        FspI        HaeII       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MluI        MluNI       MroI        MscI        
NarI        NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        RsrII       SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       StuI        SwaI        
Tth111I     Van91I      XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Alw44I       2040  GTGCAC               G^TGCAC
ApaLI        2040  GTGCAC               G^TGCAC
BcgI         1327  GCAGAAGCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI           95  TGATCA               T^GATCA
BfaI         1846  CTAG                 C^TAG
BsaAI        1143  TACGTG               [CT]AC^GT[GA]
BsaBI         742  GATCTAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI         1419  CTGCAC               GTGCAG(16/14)
BsiEI         575  CGATCG               CG[GA][CT]^CG
BsmBI        1573  GAGACG               CGTCTC(1/5)
BspMI        2027  ACCTGC               ACCTGC(4/8)
BssHII         13  GCGCGC               G^CGCGC
BssSI        2011  CACGAG               CACGAG(-5/-1)
BstAPI        713  GCAGGGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        2387  CCAAAGGAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII        1596  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DraIII       2648  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          538  GACGCCCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I     1596  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRI        2271  GAATTC               G^AATTC
EcoRV        2503  GATATC               GAT^ATC
FbaI           95  TGATCA               T^GATCA
MaeI         1846  CTAG                 C^TAG
MamI          742  GATCTAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1640  CAATTG               C^AATTG
MslI         2757  CATTATAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1640  CAATTG               C^AATTG
NaeI            3  GCCGGC               GCC^GGC
NdeI         2618  CATATG               CA^TATG
NgoMIV          3  GCCGGC               G^CCGGC
NspI         2207  GCATGC               [GA]CATG^[CT]
PvuI          575  CGATCG               CGAT^CG
SacI          210  GAGCTC               GAGCT^C
SphI         2207  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccII          14  CGCG                 CG^CG
               16  CGCG                
AcyI          538  GACGCC               G[GA]^CG[CT]C
             1347  GGCGTC              
Asp700I      2122  GAAAAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2356  GAAGATCTTC          
BanII         210  GAGCTC               G[GA]GC[CT]^C
              844  GGGCTC              
BbsI          181  GAAGAC               GAAGAC(2/6)
              506  GAAGAC              
BcnI          177  CCCGG                CC^[GC]GG
             2238  CCCGG               
BpmI         1592  CTCCAG               CTGGAG(16/14)
              413  CTGGAG              
BpuAI         181  GAAGAC               GAAGAC(2/6)
              506  GAAGAC              
BsaHI         538  GACGCC               G[GA]^CG[CT]C
             1347  GGCGTC              
BsaI          201  GAGACC               GGTCTC(1/5)
             2429  GAGACC              
BsaWI         973  ACCGGA               [AT]^CCGG[AT]
             2676  TCCGGT              
BsmFI          67  GGGAC                GGGAC(10/14)
              217  GGGAC               
BsmI          718  GAATGC               GAATGC(1/-1)
             2672  GCATTC              
BspHI         663  TCATGA               T^CATGA
             2507  TCATGA              
BsrFI           3  GCCGGC               [GA]^CCGG[CT]
             1744  ACCGGC              
BsrI          708  ACTGG                ACTGG(1/-1)
             1007  CCAGT               
BstEII       1023  GGTTACC              G^GT[ACGT]ACC
             1740  GGTGACC             
BstPI        1023  GGTTACC              G^GT[ACGT]ACC
             1740  GGTGACC             
BstUI          14  CGCG                 CG^CG
               16  CGCG                
CfoI           13  GCGC                 GCG^C
               15  GCGC                
Cfr10I          3  GCCGGC               [GA]^CCGG[CT]
             1744  ACCGGC              
EcoO65I      1023  GGTTACC              G^GT[ACGT]ACC
             1740  GGTGACC             
HgaI          538  GACGC                GACGC(5/10)
             1348  GCGTC               
HhaI           13  GCGC                 GCG^C
               15  GCGC                
Hin1I         538  GACGCC               G[GA]^CG[CT]C
             1347  GGCGTC              
HinP1I         13  GCGC                 G^CGC
               15  GCGC                
MvnI           14  CGCG                 CG^CG
               16  CGCG                
NciI          177  CCCGG                CC^[GC]GG
             2238  CCCGG               
PaeR7I        874  CTCGAG               C^TCGAG
             2498  CTCGAG              
RcaI          663  TCATGA               T^CATGA
             2507  TCATGA              
ScaI         1495  AGTACT               AGT^ACT
             1763  AGTACT              
SfcI         1726  CTACAG               C^T[GA][CT]AG
             2741  CTACAG              
XhoI          874  CTCGAG               C^TCGAG
             2498  CTCGAG              
XmnI         2122  GAAAAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2356  GAAGATCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaI        AvaII       BbvI        
BciVI       BglII       BmyI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsoBI       
Bsp1286I    BsrDI       BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraI        DsaI        EaeI        EarI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MwoI        NcoI        NdeII       NlaIII      NlaIV       
PleI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SmlI        SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com