Restriction map for pF1KE2133
 (Linear) (Six-base) MAPSORT of: KIEE2133.seq  Check: 3479  from: 1  to: 2979



KIEE2133.orf

 With 182 enzymes: SgfI * 

                        February 28, 2012 13:55  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2306    2979 
   Size:       2306     673
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1383    1415    2979 
   Size:       1383      32    1564

  Fragments arranged by size: 

                1564    1383      32
 
ApaLI G'TGCA_C

Cuts at:      0    2040    2979 
   Size:       2040     939
 
ApoI r'AATT_y

Cuts at:      0    1824    2125    2199    2271    2398    2407    2979 
   Size:       1824     301      74      72     127       9     572

  Fragments arranged by size: 

                1824     572     301     127      74      72       9
 
AvaI C'yCGr_G

Cuts at:      0     187     874    2498    2979 
   Size:        187     687    1624     481

  Fragments arranged by size: 

                1624     687     481     187
 
BanII G_rGCy'C

Cuts at:      0     214     848    2979 
   Size:        214     634    2131

  Fragments arranged by size: 

                2131     634     214
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     188     513    2979 
   Size:        188     325    2466

  Fragments arranged by size: 

                2466     325     188
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1316    1350    2979 
   Size:       1316      34    1629

  Fragments arranged by size: 

                1629    1316      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     970    1192    1360    2979 
   Size:        970     222     168    1619

  Fragments arranged by size: 

                1619     970     222     168
 
BclI T'GATC_A

Cuts at:      0      95    2979 
   Size:         95    2884
 
BglII A'GATC_T

Cuts at:      0      84     741    2358    2979 
   Size:         84     657    1617     621

  Fragments arranged by size: 

                1617     657     621      84
 
Bme1580I G_kGCm'C

Cuts at:      0    2044    2979 
   Size:       2044     935
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     718    1001    2979 
   Size:        718     283    1978

  Fragments arranged by size: 

                1978     718     283
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1514    1987    2979 
   Size:       1514     473     992

  Fragments arranged by size: 

                1514     992     473
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     434    1577    2979 
   Size:        434    1143    1402

  Fragments arranged by size: 

                1402    1143     434
 
Bpu10I CC'TnA_GC

Cuts at:      0     213    2963    2979 
   Size:        213    2750      16

  Fragments arranged by size: 

                2750     213      16
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1301    1969    2772    2979 
   Size:       1301     668     803     207

  Fragments arranged by size: 

                1301     803     668     207
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     195    2423    2979 
   Size:        195    2228     556

  Fragments arranged by size: 

                2228     556     195
 
BsaAI yAC'GTr

Cuts at:      0    1145    2979 
   Size:       1145    1834
 
BsaBI GATnn'nnATC

Cuts at:      0     746    2979 
   Size:        746    2233
 
BsaHI Gr'CG_yC

Cuts at:      0     539    1348    2979 
   Size:        539     809    1631

  Fragments arranged by size: 

                1631     809     539
 
BsaWI w'CCGG_w

Cuts at:      0     973    2676    2979 
   Size:        973    1703     303

  Fragments arranged by size: 

                1703     973     303
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     383     746     892    1645    1740    2344    2979 
   Size:        383     363     146     753      95     604     635

  Fragments arranged by size: 

                 753     635     604     383     363     146      95
 
BseYI C'CCAG_C

Cuts at:      0    1997    2001    2031    2774    2979 
   Size:       1997       4      30     743     205

  Fragments arranged by size: 

                1997     743     205      30       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1404    2979 
   Size:       1404    1575
 
BsiEI CG_ry'CG

Cuts at:      0     578    2979 
   Size:        578    2401
 
BsiHKAI G_wGCw'C

Cuts at:      0     214    1325    2044    2979 
   Size:        214    1111     719     935

  Fragments arranged by size: 

                1111     935     719     214
 
BsmI GAATG_Cn'

Cuts at:      0     724    2672    2979 
   Size:        724    1948     307

  Fragments arranged by size: 

                1948     724     307
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1567    2979 
   Size:       1567    1412
 
Bsp1286I G_dGCh'C

Cuts at:      0     214     848    1325    2044    2979 
   Size:        214     634     477     719     935

  Fragments arranged by size: 

                 935     719     634     477     214
 
BspHI T'CATG_A

Cuts at:      0     663    2507    2979 
   Size:        663    1844     472

  Fragments arranged by size: 

                1844     663     472
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2036    2979 
   Size:       2036     943
 
BsrDI GCAATG_nn'

Cuts at:      0      62     766    2544    2979 
   Size:         62     704    1778     435

  Fragments arranged by size: 

                1778     704     435      62
 
BsrFI r'CCGG_y

Cuts at:      0       3    1744    2979 
   Size:          3    1741    1235

  Fragments arranged by size: 

                1741    1235       3
 
BssHII G'CGCG_C

Cuts at:      0      13    2979 
   Size:         13    2966
 
BssSI C'ACGA_G

Cuts at:      0    2011    2979 
   Size:       2011     968
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     719    2979 
   Size:        719    2260
 
BstEII G'GTnAC_C

Cuts at:      0    1023    1740    2979 
   Size:       1023     717    1239

  Fragments arranged by size: 

                1239    1023     717
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2394    2979 
   Size:       2394     585
 
BstYI r'GATC_y

Cuts at:      0      84     741    2358    2979 
   Size:         84     657    1617     621

  Fragments arranged by size: 

                1617     657     621      84
 
BtgI C'CryG_G

Cuts at:      0     161     380     834    2460    2979 
   Size:        161     219     454    1626     519

  Fragments arranged by size: 

                1626     519     454     219     161
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2872    2979 
   Size:       2872     107
 
BtsI GCAGTG_nn'

Cuts at:      0     268    2979 
   Size:        268    2711
 
DraI TTT'AAA

Cuts at:      0    1119    1586    2718    2979 
   Size:       1119     467    1132     261

  Fragments arranged by size: 

                1132    1119     467     261
 
DraIII CAC_nnn'GTG

Cuts at:      0    2653    2979 
   Size:       2653     326
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     544    2979 
   Size:        544    2435
 
EaeI y'GGCC_r

Cuts at:      0      24    2435    2797    2877    2979 
   Size:         24    2411     362      80     102

  Fragments arranged by size: 

                2411     362     102      80      24
 
EarI CTCTTCn'nnn_

Cuts at:      0     999    1088    1421    1663    2321    2979 
   Size:        999      89     333     242     658     658

  Fragments arranged by size: 

                 999     658     658     333     242      89
 
EcoICRI GAG'CTC

Cuts at:      0     212    2979 
   Size:        212    2767
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     434    1577    2306    2979 
   Size:        434    1143     729     673

  Fragments arranged by size: 

                1143     729     673     434
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1597    2979 
   Size:       1597    1382
 
EcoRI G'AATT_C

Cuts at:      0    2271    2979 
   Size:       2271     708
 
EcoRV GAT'ATC

Cuts at:      0    2505    2979 
   Size:       2505     474
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     527    2380    2979 
   Size:        527    1853     599

  Fragments arranged by size: 

                1853     599     527
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     830     862    1140    1172    1609    1641    1898    1930 
   Size:        830      32     278      32     437      32     257      32

Cuts at:   1930    2239    2271    2599    2631    2979 
   Size:        309      32     328      32     348

  Fragments arranged by size: 

                 830     437     348     328     309     278     257      32
                  32      32      32      32      32
 
MfeI C'AATT_G

Cuts at:      0    1640    2979 
   Size:       1640    1339
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2543    2979 
   Size:       2543     436
 
MslI CAynn'nnrTG

Cuts at:      0    2761    2979 
   Size:       2761     218
 
MspA1I CmG'CkG

Cuts at:      0      30      36     238     634    2001    2838    2979 
   Size:         30       6     202     396    1367     837     141

  Fragments arranged by size: 

                1367     837     396     202     141      30       6
 
NaeI GCC'GGC

Cuts at:      0       5    2979 
   Size:          5    2974
 
NcoI C'CATG_G

Cuts at:      0     161     380     834    2979 
   Size:        161     219     454    2145

  Fragments arranged by size: 

                2145     454     219     161
 
NdeI CA'TA_TG

Cuts at:      0    2619    2979 
   Size:       2619     360
 
NgoMIV G'CCGG_C

Cuts at:      0       3    2979 
   Size:          3    2976
 
NspI r_CATG'y

Cuts at:      0    2211    2979 
   Size:       2211     768
 
PasI CC'CwG_GG

Cuts at:      0     292    2979 
   Size:        292    2687
 
PsiI TTA'TAA

Cuts at:      0    1048    2761    2979 
   Size:       1048    1713     218

  Fragments arranged by size: 

                1713    1048     218
 
PspXI vC'TCGA_Gb

Cuts at:      0     874    2979 
   Size:        874    2105
 
PvuI CG_AT'CG

Cuts at:      0     578    2979 
   Size:        578    2401
 
PvuII CAG'CTG

Cuts at:      0      30     634    2001    2838    2979 
   Size:         30     604    1367     837     141

  Fragments arranged by size: 

                1367     837     604     141      30
 
SacI G_AGCT'C

Cuts at:      0     214    2979 
   Size:        214    2765
 
ScaI AGT'ACT

Cuts at:      0    1497    1765    2979 
   Size:       1497     268    1214

  Fragments arranged by size: 

                1497    1214     268
 
SfcI C'TryA_G

Cuts at:      0    1726    2741    2979 
   Size:       1726    1015     238

  Fragments arranged by size: 

                1726    1015     238
 
SmlI C'TyrA_G

Cuts at:      0     874    1316    1984    2498    2787    2979 
   Size:        874     442     668     514     289     192

  Fragments arranged by size: 

                 874     668     514     442     289     192
 
SphI G_CATG'C

Cuts at:      0    2211    2979 
   Size:       2211     768
 
SspI AAT'ATT

Cuts at:      0     461    2019    2627    2979 
   Size:        461    1558     608     352

  Fragments arranged by size: 

                1558     608     461     352
 
StyI C'CwwG_G

Cuts at:      0     161     380     834    1557    2979 
   Size:        161     219     454     723    1422

  Fragments arranged by size: 

                1422     723     454     219     161
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     576    1473    2473    2979 
   Size:        576     897    1000     506

  Fragments arranged by size: 

                1000     897     576     506
 
TatI w'GTAC_w

Cuts at:      0    1495    1694    1763    2979 
   Size:       1495     199      69    1216

  Fragments arranged by size: 

                1495    1216     199      69
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     673     705    1469    1501    2979 
   Size:        673      32     764      32    1478

  Fragments arranged by size: 

                1478     764     673      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     705    1379    1385    2979 
   Size:        705     674       6    1594

  Fragments arranged by size: 

                1594     705     674       6
 
XhoI C'TCGA_G

Cuts at:      0     874    2498    2979 
   Size:        874    1624     481

  Fragments arranged by size: 

                1624     874     481
 
XmnI GAAnn'nnTTC

Cuts at:      0    2126    2360    2979 
   Size:       2126     234     619

  Fragments arranged by size: 

                2126     619     234
 

 Enzymes that do cut:

     AcuI     AloI    ApaLI     ApoI     AvaI    BanII     BbsI     BcgI
    BciVI     BclI    BglII Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EarI
  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI    Hin4I     MfeI
     MmeI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NspI
     PasI     PsiI    PspXI     PvuI    PvuII     SacI     ScaI     SfcI
     SmlI     SphI     SspI     StyI    TaqII     TatI     TstI     XcmI
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI    BfrBI
     BglI     BlpI    BmgBI     BmtI    BsaXI    BsiWI    BspEI    BsrBI
    BsrGI    BstBI  BstZ17I   Bsu36I     ClaI    CspCI     EagI     EciI
    EcoNI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MscI     NarI     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI     PsrI     PstI    RsrII    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI  Tth111I     XbaI
     XmaI     ZraI
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