Restriction map for
pF1KE9396
(Linear) (Six-base) MAPSORT of: KIEE9396.seq Check: 4266 from: 1 to: 11907
KIEE9396.orf
With 182 enzymes: SgfI *
May 26, 2016 12:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 11125 11542 11907
Size: 11125 417 365
Fragments arranged by size:
11125 417 365
AatII G_ACGT'C
Cuts at: 0 3450 11907
Size: 3450 8457
AccI GT'mk_AC
Cuts at: 0 1333 5037 6161 6477 8841 9675 10460 11498
Size: 1333 3704 1124 316 2364 834 785 1038
Cuts at: 11498 11907
Size: 409
Fragments arranged by size:
3704 2364 1333 1124 1038 834 785 409
316
Acc65I G'GTAC_C
Cuts at: 0 2011 9096 10111 11907
Size: 2011 7085 1015 1796
Fragments arranged by size:
7085 2011 1796 1015
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 482 1403 1541 2006 2168 4951 6839
Size: 284 198 921 138 465 162 2783 1888
Cuts at: 6839 6863 7049 7211 8003 8054 8294 8522 8975
Size: 24 186 162 792 51 240 228 453
Cuts at: 8975 9076 9232 9341 9527 10339 10409 10568 10715
Size: 101 156 109 186 812 70 159 147
Cuts at: 10715 10933 11318 11428 11907
Size: 218 385 110 479
Fragments arranged by size:
2783 1888 921 812 792 479 465 453
385 284 240 228 218 198 186 186
162 162 159 156 147 138 110 109
101 70 51 24
AfeI AGC'GCT
Cuts at: 0 6190 11907
Size: 6190 5717
AflII C'TTAA_G
Cuts at: 0 8010 11907
Size: 8010 3897
AflIII A'CryG_T
Cuts at: 0 99 3207 5289 5697 7877 8295 10080 11327
Size: 99 3108 2082 408 2180 418 1785 1247
Cuts at: 11327 11907
Size: 580
Fragments arranged by size:
3108 2180 2082 1785 1247 580 418 408
99
AhdI GACnn_n'nnGTC
Cuts at: 0 6888 11907
Size: 6888 5019
AleI CACnn'nnGTG
Cuts at: 0 4451 4861 5164 11723 11907
Size: 4451 410 303 6559 184
Fragments arranged by size:
6559 4451 410 303 184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 391 2477 4133 6890 9472 10297 11455 11714
Size: 391 2086 1656 2757 2582 825 1158 259
Cuts at: 11714 11878 11907
Size: 164 29
Fragments arranged by size:
2757 2582 2086 1656 1158 825 391 259
164 29
AlwNI CAG_nnn'CTG
Cuts at: 0 4485 7684 8700 8716 9223 9988 10167 10762
Size: 4485 3199 1016 16 507 765 179 595
Cuts at: 10762 11907
Size: 1145
Fragments arranged by size:
4485 3199 1145 1016 765 595 507 179
16
ApaI G_GGCC'C
Cuts at: 0 131 328 10433 11907
Size: 131 197 10105 1474
Fragments arranged by size:
10105 1474 197 131
ApaLI G'TGCA_C
Cuts at: 0 7865 11907
Size: 7865 4042
ApoI r'AATT_y
Cuts at: 0 555 2693 4175 4438 4956 5800 6095 6750
Size: 555 2138 1482 263 518 844 295 655
Cuts at: 6750 6792 6980 8454 11015 11230 11907
Size: 42 188 1474 2561 215 677
Fragments arranged by size:
2561 2138 1482 1474 844 677 655 555
518 295 263 215 188 42
AseI AT'TA_AT
Cuts at: 0 959 5213 11907
Size: 959 4254 6694
Fragments arranged by size:
6694 4254 959
AvaI C'yCGr_G
Cuts at: 0 37 124 151 327 1972 2563 5020 5876
Size: 37 87 27 176 1645 591 2457 856
Cuts at: 5876 11744 11907
Size: 5868 163
Fragments arranged by size:
5868 2457 1645 856 591 176 163 87
37 27
AvrII C'CTAG_G
Cuts at: 0 78 11907
Size: 78 11829
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 7178 7211 8171 8204 8421 8454 9606 9639
Size: 7178 33 960 33 217 33 1152 33
Cuts at: 9639 11907
Size: 2268
Fragments arranged by size:
7178 2268 1152 960 217 33 33 33
33
BamHI G'GATC_C
Cuts at: 0 3750 4609 9576 11907
Size: 3750 859 4967 2331
Fragments arranged by size:
4967 3750 2331 859
BanI G'GyrC_C
Cuts at: 0 68 85 156 367 2011 3417 3486 4880
Size: 68 17 71 211 1644 1406 69 1394
Cuts at: 4880 7539 9096 9894 10111 11206 11878 11907
Size: 2659 1557 798 217 1095 672 29
Fragments arranged by size:
2659 1644 1557 1406 1394 1095 798 672
217 211 71 69 68 29 17
BanII G_rGCy'C
Cuts at: 0 131 328 1149 2083 2097 2300 3980 4361
Size: 131 197 821 934 14 203 1680 381
Cuts at: 4361 6901 6946 8483 8599 8935 10433 11907
Size: 2540 45 1537 116 336 1498 1474
Fragments arranged by size:
2540 1680 1537 1498 1474 934 821 381
336 203 197 131 116 45 14
BbeI G_GCGC'C
Cuts at: 0 72 89 160 11882 11907
Size: 72 17 71 11722 25
Fragments arranged by size:
11722 72 71 25 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 263 269 675 1204 1409 2312 2798 3308
Size: 263 6 406 529 205 903 486 510
Cuts at: 3308 5573 5609 6925 7934 8033 11907
Size: 2265 36 1316 1009 99 3874
Fragments arranged by size:
3874 2265 1316 1009 903 529 510 486
406 263 205 99 36 6
BbvCI CC'TCA_GC
Cuts at: 0 243 291 1899 3920 4138 10506 11907
Size: 243 48 1608 2021 218 6368 1401
Fragments arranged by size:
6368 2021 1608 1401 243 218 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 29 236 270 11907
Size: 29 207 34 11637
Fragments arranged by size:
11637 207 34 29
BciVI GTATCCnnnnn_n'
Cuts at: 0 2202 2726 2847 4066 7856 9957 10041 10449
Size: 2202 524 121 1219 3790 2101 84 408
Cuts at: 10449 10767 11907
Size: 318 1140
Fragments arranged by size:
3790 2202 2101 1219 1140 524 408 318
121 84
BclI T'GATC_A
Cuts at: 0 5103 5774 5939 6729 8973 11907
Size: 5103 671 165 790 2244 2934
Fragments arranged by size:
5103 2934 2244 790 671 165
BfrBI ATG'CAT
Cuts at: 0 11691 11907
Size: 11691 216
BglI GCCn_nnn'nGGC
Cuts at: 0 83 190 9937 11907
Size: 83 107 9747 1970
Fragments arranged by size:
9747 1970 107 83
BglII A'GATC_T
Cuts at: 0 2245 5118 9862 11039 11798 11907
Size: 2245 2873 4744 1177 759 109
Fragments arranged by size:
4744 2873 2245 1177 759 109
BlpI GC'TnA_GC
Cuts at: 0 1115 1175 3095 8396 8401 8929 10763 11286
Size: 1115 60 1920 5301 5 528 1834 523
Cuts at: 11286 11907
Size: 621
Fragments arranged by size:
5301 1920 1834 1115 621 528 523 60
5
Bme1580I G_kGCm'C
Cuts at: 0 131 328 1359 1656 7863 7869 9143 10433
Size: 131 197 1031 297 6207 6 1274 1290
Cuts at: 10433 11907
Size: 1474
Fragments arranged by size:
6207 1474 1290 1274 1031 297 197 131
6
BmgBI CAC'GTC
Cuts at: 0 10083 11907
Size: 10083 1824
BmrI ACTGGGnnnn_n'
Cuts at: 0 1439 1542 6437 6653 7964 8831 9329 10234
Size: 1439 103 4895 216 1311 867 498 905
Cuts at: 10234 10397 11907
Size: 163 1510
Fragments arranged by size:
4895 1510 1439 1311 905 867 498 216
163 103
BmtI G_CTAG'C
Cuts at: 0 9449 10157 10784 11907
Size: 9449 708 627 1123
Fragments arranged by size:
9449 1123 708 627
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2139 7110 7845 9400 10812 11833 11907
Size: 2139 4971 735 1555 1412 1021 74
Fragments arranged by size:
4971 2139 1555 1412 1021 735 74
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 707 1710 2968 3416 3848 4420 4507
Size: 638 69 1003 1258 448 432 572 87
Cuts at: 4507 4604 4991 5516 6186 6212 6558 7160 10504
Size: 97 387 525 670 26 346 602 3344
Cuts at: 10504 10733 11907
Size: 229 1174
Fragments arranged by size:
3344 1258 1174 1003 670 638 602 572
525 448 432 387 346 229 97 87
69 26
Bpu10I CC'TnA_GC
Cuts at: 0 243 291 1773 1899 3032 3920 4138 4257
Size: 243 48 1482 126 1133 888 218 119
Cuts at: 4257 6090 10506 11907
Size: 1833 4416 1401
Fragments arranged by size:
4416 1833 1482 1401 1133 888 243 218
126 119 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 6036 9637 11907
Size: 826 5210 3601 2270
Fragments arranged by size:
5210 3601 2270 826
BsaI GGTCTCn'nnnn_
Cuts at: 0 357 497 525 601 955 2436 2621 3172
Size: 357 140 28 76 354 1481 185 551
Cuts at: 3172 3897 4652 7130 9388 11510 11907
Size: 725 755 2478 2258 2122 397
Fragments arranged by size:
2478 2258 2122 1481 755 725 551 397
357 354 185 140 76 28
BsaBI GATnn'nnATC
Cuts at: 0 6849 9867 11907
Size: 6849 3018 2040
Fragments arranged by size:
6849 3018 2040
BsaHI Gr'CG_yC
Cuts at: 0 69 86 157 3447 6579 11879 11907
Size: 69 17 71 3290 3132 5300 28
Fragments arranged by size:
5300 3290 3132 71 69 28 17
BsaWI w'CCGG_w
Cuts at: 0 989 4098 6620 9573 10842 11907
Size: 989 3109 2522 2953 1269 1065
Fragments arranged by size:
3109 2953 2522 1269 1065 989
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 477 507 2448 2478 5810 5840 8107 8137
Size: 477 30 1941 30 3332 30 2267 30
Cuts at: 8137 10731 10761 11907
Size: 2594 30 1146
Fragments arranged by size:
3332 2594 2267 1941 1146 477 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 243 827 943 1242 1395 1416 1495 1516
Size: 243 584 116 299 153 21 79 21
Cuts at: 1516 1662 2489 2581 3773 4396 5044 5507 6365
Size: 146 827 92 1192 623 648 463 858
Cuts at: 6365 6540 6891 7161 8128 8895 9560 11266 11407
Size: 175 351 270 967 767 665 1706 141
Cuts at: 11407 11410 11822 11907
Size: 3 412 85
Fragments arranged by size:
1706 1192 967 858 827 767 665 648
623 584 463 412 351 299 270 243
175 153 146 141 116 92 85 79
21 21 3
BseYI C'CCAG_C
Cuts at: 0 214 1658 3896 4778 6605 8281 8824 8919
Size: 214 1444 2238 882 1827 1676 543 95
Cuts at: 8919 9779 10403 11157 11907
Size: 860 624 754 750
Fragments arranged by size:
2238 1827 1676 1444 882 860 754 750
624 543 214 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1409 5832 6941 10907 11066 11687 11907
Size: 1409 4423 1109 3966 159 621 220
Fragments arranged by size:
4423 3966 1409 1109 621 220 159
BsiEI CG_ry'CG
Cuts at: 0 137 185 236 359 3454 6481 11907
Size: 137 48 51 123 3095 3027 5426
Fragments arranged by size:
5426 3095 3027 137 123 51 48
BsiHKAI G_wGCw'C
Cuts at: 0 2097 2300 2466 6172 6901 6946 7869 8483
Size: 2097 203 166 3706 729 45 923 614
Cuts at: 8483 10286 11907
Size: 1803 1621
Fragments arranged by size:
3706 2097 1803 1621 923 729 614 203
166 45
BsmI GAATG_Cn'
Cuts at: 0 1869 4122 7320 11907
Size: 1869 2253 3198 4587
Fragments arranged by size:
4587 3198 2253 1869
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1682 9173 11907
Size: 1682 7491 2734
Fragments arranged by size:
7491 2734 1682
Bsp1286I G_dGCh'C
Cuts at: 0 131 328 1149 1359 1656 2083 2097 2300
Size: 131 197 821 210 297 427 14 203
Cuts at: 2300 2466 3980 4361 6172 6901 6946 7863 7869
Size: 166 1514 381 1811 729 45 917 6
Cuts at: 7869 8483 8599 8935 9143 10286 10433 11907
Size: 614 116 336 208 1143 147 1474
Fragments arranged by size:
1811 1514 1474 1143 917 821 729 614
427 381 336 297 210 208 203 197
166 147 131 116 45 14 6
BspEI T'CCGG_A
Cuts at: 0 989 6620 9573 10842 11907
Size: 989 5631 2953 1269 1065
Fragments arranged by size:
5631 2953 1269 1065 989
BspHI T'CATG_A
Cuts at: 0 4650 4668 6516 11907
Size: 4650 18 1848 5391
Fragments arranged by size:
5391 4650 1848 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 339 383 814 3623 3879 10827 11125 11542
Size: 339 44 431 2809 256 6948 298 417
Cuts at: 11542 11907
Size: 365
Fragments arranged by size:
6948 2809 431 417 365 339 298 256
44
BsrBI CCG'CTC
Cuts at: 0 232 407 1506 4878 11591 11907
Size: 232 175 1099 3372 6713 316
Fragments arranged by size:
6713 3372 1099 316 232 175
BsrDI GCAATG_nn'
Cuts at: 0 1109 4765 6281 9725 11907
Size: 1109 3656 1516 3444 2182
Fragments arranged by size:
3656 3444 2182 1516 1109
BsrFI r'CCGG_y
Cuts at: 0 306 380 4988 9891 11575 11907
Size: 306 74 4608 4903 1684 332
Fragments arranged by size:
4903 4608 1684 332 306 74
BssSI C'ACGA_G
Cuts at: 0 524 11721 11907
Size: 524 11197 186
Fragments arranged by size:
11197 524 186
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1111 1909 3229 11642 11907
Size: 1111 798 1320 8413 265
Fragments arranged by size:
8413 1320 1111 798 265
BstBI TT'CG_AA
Cuts at: 0 6435 6561 11907
Size: 6435 126 5346
Fragments arranged by size:
6435 5346 126
BstEII G'GTnAC_C
Cuts at: 0 10854 11907
Size: 10854 1053
BstXI CCAn_nnnn'nTGG
Cuts at: 0 5256 9342 11907
Size: 5256 4086 2565
Fragments arranged by size:
5256 4086 2565
BstYI r'GATC_y
Cuts at: 0 573 1369 1615 2245 3750 4574 4609 5118
Size: 573 796 246 630 1505 824 35 509
Cuts at: 5118 6463 6844 6952 7255 7519 7912 8943 9576
Size: 1345 381 108 303 264 393 1031 633
Cuts at: 9576 9862 11039 11436 11798 11907
Size: 286 1177 397 362 109
Fragments arranged by size:
1505 1345 1177 1031 824 796 633 630
573 509 397 393 381 362 303 286
264 246 109 108 35
BstZ17I GTA'TAC
Cuts at: 0 5038 6162 11907
Size: 5038 1124 5745
Fragments arranged by size:
5745 5038 1124
Bsu36I CC'TnA_GG
Cuts at: 0 1499 3491 7817 10734 11308 11907
Size: 1499 1992 4326 2917 574 599
Fragments arranged by size:
4326 2917 1992 1499 599 574
BtgI C'CryG_G
Cuts at: 0 91 185 3289 3320 5299 5825 7543 9371
Size: 91 94 3104 31 1979 526 1718 1828
Cuts at: 9371 11514 11907
Size: 2143 393
Fragments arranged by size:
3104 2143 1979 1828 1718 526 393 94
91 31
BtsI GCAGTG_nn'
Cuts at: 0 1534 1711 1799 3475 3565 3699 5381 7863
Size: 1534 177 88 1676 90 134 1682 2482
Cuts at: 7863 8923 11907
Size: 1060 2984
Fragments arranged by size:
2984 2482 1682 1676 1534 1060 177 134
90 88
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1729 1764 3519 3554 4354 4389 5525 5560
Size: 1729 35 1755 35 800 35 1136 35
Cuts at: 5560 5671 5706 11907
Size: 111 35 6201
Fragments arranged by size:
6201 1755 1729 1136 800 111 35 35
35 35 35
DraI TTT'AAA
Cuts at: 0 1865 6908 7836 11907
Size: 1865 5043 928 4071
Fragments arranged by size:
5043 4071 1865 928
DraIII CAC_nnn'GTG
Cuts at: 0 9337 9913 11907
Size: 9337 576 1994
Fragments arranged by size:
9337 1994 576
DrdI GACnn_nn'nnGTC
Cuts at: 0 1674 11907
Size: 1674 10233
EaeI y'GGCC_r
Cuts at: 0 182 233 356 3237 5679 8316 11517 11907
Size: 182 51 123 2881 2442 2637 3201 390
Fragments arranged by size:
3201 2881 2637 2442 390 182 123 51
EagI C'GGCC_G
Cuts at: 0 182 233 356 11907
Size: 182 51 123 11551
Fragments arranged by size:
11551 182 123 51
EarI CTCTTCn'nnn_
Cuts at: 0 303 655 2090 2334 2509 3109 3213 5979
Size: 303 352 1435 244 175 600 104 2766
Cuts at: 5979 6597 6792 7068 8739 8866 9284 9321 10904
Size: 618 195 276 1671 127 418 37 1583
Cuts at: 10904 11384 11907
Size: 480 523
Fragments arranged by size:
2766 1671 1583 1435 618 600 523 480
418 352 303 276 244 195 175 127
104 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 252 2811 11907
Size: 252 2559 9096
Fragments arranged by size:
9096 2559 252
EcoICRI GAG'CTC
Cuts at: 0 2095 2298 6899 6944 8481 11907
Size: 2095 203 4601 45 1537 3426
Fragments arranged by size:
4601 3426 2095 1537 203 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 482 638 707 1403 1541 1710 2006
Size: 284 198 156 69 696 138 169 296
Cuts at: 2006 2168 2968 3416 3848 4420 4507 4604 4951
Size: 162 800 448 432 572 87 97 347
Cuts at: 4951 4991 5516 6186 6212 6558 6839 6863 7049
Size: 40 525 670 26 346 281 24 186
Cuts at: 7049 7160 7211 8003 8054 8294 8522 8975 9076
Size: 111 51 792 51 240 228 453 101
Cuts at: 9076 9232 9341 9527 10339 10409 10504 10568 10715
Size: 156 109 186 812 70 95 64 147
Cuts at: 10715 10733 10933 11318 11428 11907
Size: 18 200 385 110 479
Fragments arranged by size:
812 800 792 696 670 572 525 479
453 448 432 385 347 346 296 284
281 240 228 200 198 186 186 169
162 156 156 147 138 111 110 109
101 97 95 87 70 69 64 51
51 40 26 24 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1811 2950 3082 3932 6491 9542 11907
Size: 1811 1139 132 850 2559 3051 2365
Fragments arranged by size:
3051 2559 2365 1811 1139 850 132
EcoO109I rG'GnC_Cy
Cuts at: 0 75 128 2559 3193 5062 5491 6423 7089
Size: 75 53 2431 634 1869 429 932 666
Cuts at: 7089 9367 10429 10430 10594 11907
Size: 2278 1062 1 164 1313
Fragments arranged by size:
2431 2278 1869 1313 1062 932 666 634
429 164 75 53 1
EcoRI G'AATT_C
Cuts at: 0 4956 5800 6095 6750 6980 11907
Size: 4956 844 295 655 230 4927
Fragments arranged by size:
4956 4927 844 655 295 230
EcoRV GAT'ATC
Cuts at: 0 6145 6246 11907
Size: 6145 101 5661
Fragments arranged by size:
6145 5661 101
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 512 794 1140 1409 1939 2267 5286 11907
Size: 512 282 346 269 530 328 3019 6621
Fragments arranged by size:
6621 3019 530 512 346 328 282 269
FseI GG_CCGG'CC
Cuts at: 0 310 11907
Size: 310 11597
HaeII r_GCGC'y
Cuts at: 0 24 72 89 160 346 4395 6192 11882
Size: 24 48 17 71 186 4049 1797 5690
Cuts at: 11882 11907
Size: 25
Fragments arranged by size:
5690 4049 1797 186 71 48 25 24
17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 598 611 630 643 680 712 1211 1243
Size: 598 13 19 13 37 32 499 32
Cuts at: 1243 2448 2480 2557 2589 2886 2918 3108 3140
Size: 1205 32 77 32 297 32 190 32
Cuts at: 3140 3322 3325 3354 3357 3564 3596 3640 3672
Size: 182 3 29 3 207 32 44 32
Cuts at: 3672 4813 4845 5731 5763 6615 6647 6694 6726
Size: 1141 32 886 32 852 32 47 32
Cuts at: 6726 6874 6906 7585 7617 7642 7674 8767 8799
Size: 148 32 679 32 25 32 1093 32
Cuts at: 8799 8867 8899 8958 8990 8995 9027 9799 9831
Size: 68 32 59 32 5 32 772 32
Cuts at: 9831 10111 10143 10848 10880 11185 11217 11907
Size: 280 32 705 32 305 32 690
Fragments arranged by size:
1205 1141 1093 886 852 772 705 690
679 598 499 305 297 280 207 190
182 148 77 68 59 47 44 37
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 29
25 19 13 13 5 3 3
HincII GTy'rAC
Cuts at: 0 6478 7655 9243 11142 11907
Size: 6478 1177 1588 1899 765
Fragments arranged by size:
6478 1899 1588 1177 765
HindIII A'AGCT_T
Cuts at: 0 865 3043 9411 11907
Size: 865 2178 6368 2496
Fragments arranged by size:
6368 2496 2178 865
HpaI GTT'AAC
Cuts at: 0 9243 11142 11907
Size: 9243 1899 765
Fragments arranged by size:
9243 1899 765
KasI G'GCGC_C
Cuts at: 0 68 85 156 11878 11907
Size: 68 17 71 11722 29
Fragments arranged by size:
11722 71 68 29 17
KpnI G_GTAC'C
Cuts at: 0 2015 9100 10115 11907
Size: 2015 7085 1015 1792
Fragments arranged by size:
7085 2015 1792 1015
MfeI C'AATT_G
Cuts at: 0 3344 9307 11907
Size: 3344 5963 2600
Fragments arranged by size:
5963 3344 2600
MluI A'CGCG_T
Cuts at: 0 99 11907
Size: 99 11808
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1580 1786 1977 5150 6137 6319 6478 8129
Size: 1580 206 191 3173 987 182 159 1651
Cuts at: 8129 8471 8971 10466 11907
Size: 342 500 1495 1441
Fragments arranged by size:
3173 1651 1580 1495 1441 987 500 342
206 191 182 159
MscI TGG'CCA
Cuts at: 0 5681 8318 11907
Size: 5681 2637 3589
Fragments arranged by size:
5681 3589 2637
MslI CAynn'nnrTG
Cuts at: 0 1851 4451 4861 5164 5402 6839 8324 8996
Size: 1851 2600 410 303 238 1437 1485 672
Cuts at: 8996 10279 11696 11723 11907
Size: 1283 1417 27 184
Fragments arranged by size:
2600 1851 1485 1437 1417 1283 672 410
303 238 184 27
MspA1I CmG'CkG
Cuts at: 0 11 93 187 209 425 1179 2017 2863
Size: 11 82 94 22 216 754 838 846
Cuts at: 2863 3226 4410 4482 5722 5790 6340 6609 7981
Size: 363 1184 72 1240 68 550 269 1372
Cuts at: 7981 8700 9187 9937 10264 10510 10569 10632 11199
Size: 719 487 750 327 246 59 63 567
Cuts at: 11199 11406 11907
Size: 207 501
Fragments arranged by size:
1372 1240 1184 846 838 754 750 719
567 550 501 487 363 327 269 246
216 207 94 82 72 68 63 59
22 11
NaeI GCC'GGC
Cuts at: 0 308 11577 11907
Size: 308 11269 330
Fragments arranged by size:
11269 330 308
NarI GG'CG_CC
Cuts at: 0 69 86 157 11879 11907
Size: 69 17 71 11722 28
Fragments arranged by size:
11722 71 69 28 17
NcoI C'CATG_G
Cuts at: 0 3289 3320 5299 9371 11514 11907
Size: 3289 31 1979 4072 2143 393
Fragments arranged by size:
4072 3289 2143 1979 393 31
NdeI CA'TA_TG
Cuts at: 0 5765 7250 11907
Size: 5765 1485 4657
Fragments arranged by size:
5765 4657 1485
NgoMIV G'CCGG_C
Cuts at: 0 306 11575 11907
Size: 306 11269 332
Fragments arranged by size:
11269 332 306
NheI G'CTAG_C
Cuts at: 0 9445 10153 10780 11907
Size: 9445 708 627 1127
Fragments arranged by size:
9445 1127 708 627
NotI GC'GGCC_GC
Cuts at: 0 182 233 11907
Size: 182 51 11674
Fragments arranged by size:
11674 182 51
NsiI A_TGCA'T
Cuts at: 0 11693 11907
Size: 11693 214
NspI r_CATG'y
Cuts at: 0 3211 3270 4327 5701 6206 7881 8299 9255
Size: 3211 59 1057 1374 505 1675 418 956
Cuts at: 9255 10363 11331 11907
Size: 1108 968 576
Fragments arranged by size:
3211 1675 1374 1108 1057 968 956 576
505 418 59
PasI CC'CwG_GG
Cuts at: 0 3835 4289 11907
Size: 3835 454 7618
Fragments arranged by size:
7618 3835 454
PciI A'CATG_T
Cuts at: 0 3207 5697 7877 8295 11327 11907
Size: 3207 2490 2180 418 3032 580
Fragments arranged by size:
3207 3032 2490 2180 580 418
PflMI CCAn_nnn'nTGG
Cuts at: 0 4186 5327 7699 7903 9588 9646 10123 10188
Size: 4186 1141 2372 204 1685 58 477 65
Cuts at: 10188 10561 11907
Size: 373 1346
Fragments arranged by size:
4186 2372 1685 1346 1141 477 373 204
65 58
PfoI T'CCnGG_A
Cuts at: 0 3871 5493 7692 10074 10749 11090 11907
Size: 3871 1622 2199 2382 675 341 817
Fragments arranged by size:
3871 2382 2199 1622 817 675 341
PpuMI rG'GwC_Cy
Cuts at: 0 3193 5062 5491 6423 9367 10594 11907
Size: 3193 1869 429 932 2944 1227 1313
Fragments arranged by size:
3193 2944 1869 1313 1227 932 429
PshAI GACnn'nnGTC
Cuts at: 0 1039 1204 3719 8672 10569 11907
Size: 1039 165 2515 4953 1897 1338
Fragments arranged by size:
4953 2515 1897 1338 1039 165
PsiI TTA'TAA
Cuts at: 0 2884 6349 11907
Size: 2884 3465 5558
Fragments arranged by size:
5558 3465 2884
PspOMI G'GGCC_C
Cuts at: 0 127 324 10429 11907
Size: 127 197 10105 1478
Fragments arranged by size:
10105 1478 197 127
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 6633 6665 11907
Size: 6633 32 5242
Fragments arranged by size:
6633 5242 32
PstI C_TGCA'G
Cuts at: 0 350 1184 1718 2430 3217 3620 4890 4922
Size: 350 834 534 712 787 403 1270 32
Cuts at: 4922 6495 9501 10824 11907
Size: 1573 3006 1323 1083
Fragments arranged by size:
3006 1573 1323 1270 1083 834 787 712
534 403 350 32
PvuI CG_AT'CG
Cuts at: 0 3454 11907
Size: 3454 8453
PvuII CAG'CTG
Cuts at: 0 11 1179 2863 3226 4410 4482 5790 6340
Size: 11 1168 1684 363 1184 72 1308 550
Cuts at: 6340 7981 8700 9187 10510 10632 11199 11406 11907
Size: 1641 719 487 1323 122 567 207 501
Fragments arranged by size:
1684 1641 1323 1308 1184 1168 719 567
550 501 487 363 207 122 72 11
SacI G_AGCT'C
Cuts at: 0 2097 2300 6901 6946 8483 11907
Size: 2097 203 4601 45 1537 3424
Fragments arranged by size:
4601 3424 2097 1537 203 45
SacII CC_GC'GG
Cuts at: 0 94 188 11907
Size: 94 94 11719
Fragments arranged by size:
11719 94 94
SalI G'TCGA_C
Cuts at: 0 6476 11907
Size: 6476 5431
SanDI GG'GwC_CC
Cuts at: 0 9367 10594 11907
Size: 9367 1227 1313
Fragments arranged by size:
9367 1313 1227
SapI GCTCTTCn'nnn_
Cuts at: 0 3213 11907
Size: 3213 8694
SbfI CC_TGCA'GG
Cuts at: 0 10824 11907
Size: 10824 1083
ScaI AGT'ACT
Cuts at: 0 5550 6759 11907
Size: 5550 1209 5148
Fragments arranged by size:
5550 5148 1209
SexAI A'CCwGG_T
Cuts at: 0 7344 11907
Size: 7344 4563
SfcI C'TryA_G
Cuts at: 0 346 1180 1714 2426 3213 3616 3932 4886
Size: 346 834 534 712 787 403 316 954
Cuts at: 4886 4918 6491 6815 7941 8897 9497 9676 10820
Size: 32 1573 324 1126 956 600 179 1144
Cuts at: 10820 11499 11907
Size: 679 408
Fragments arranged by size:
1573 1144 1126 956 954 834 787 712
679 600 534 408 403 346 324 316
179 32
SfoI GGC'GCC
Cuts at: 0 70 87 158 11880 11907
Size: 70 17 71 11722 27
Fragments arranged by size:
11722 71 70 27 17
SmaI CCC'GGG
Cuts at: 0 39 126 153 329 11907
Size: 39 87 27 176 11578
Fragments arranged by size:
11578 176 87 39 27
SmlI C'TyrA_G
Cuts at: 0 841 6015 8010 9652 11907
Size: 841 5174 1995 1642 2255
Fragments arranged by size:
5174 2255 1995 1642 841
SpeI A'CTAG_T
Cuts at: 0 508 2365 2458 6760 8182 11907
Size: 508 1857 93 4302 1422 3725
Fragments arranged by size:
4302 3725 1857 1422 508 93
SrfI GCCC'GGGC
Cuts at: 0 329 11907
Size: 329 11578
SspI AAT'ATT
Cuts at: 0 1215 1940 2132 11757 11907
Size: 1215 725 192 9625 150
Fragments arranged by size:
9625 1215 725 192 150
StuI AGG'CCT
Cuts at: 0 915 7472 9548 11907
Size: 915 6557 2076 2359
Fragments arranged by size:
6557 2359 2076 915
StyI C'CwwG_G
Cuts at: 0 78 1747 2446 2509 3234 3289 3320 5148
Size: 78 1669 699 63 725 55 31 1828
Cuts at: 5148 5299 7526 8387 8985 9371 10099 11514 11907
Size: 151 2227 861 598 386 728 1415 393
Fragments arranged by size:
2227 1828 1669 1415 861 728 725 699
598 393 386 151 78 63 55 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 566 3396 4302 6383 6456 7518 8578 9348
Size: 566 2830 906 2081 73 1062 1060 770
Cuts at: 9348 11907
Size: 2559
Fragments arranged by size:
2830 2559 2081 1062 1060 906 770 566
73
TatI w'GTAC_w
Cuts at: 0 3508 4870 5548 6757 7776 8556 11907
Size: 3508 1362 678 1209 1019 780 3351
Fragments arranged by size:
3508 3351 1362 1209 1019 780 678
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 477 509 1178 1210 2259 2291 3896 3928
Size: 477 32 669 32 1049 32 1605 32
Cuts at: 3928 4405 4437 4627 4659 5810 5842 8107 8139
Size: 477 32 190 32 1151 32 2265 32
Cuts at: 8139 8160 8192 8796 8828 9472 9504 10654 10686
Size: 21 32 604 32 644 32 1150 32
Cuts at: 10686 10731 10763 11907
Size: 45 32 1144
Fragments arranged by size:
2265 1605 1151 1150 1144 1049 669 644
604 477 477 190 45 32 32 32
32 32 32 32 32 32 32 32
32 32 21
Tth111I GACn'n_nGTC
Cuts at: 0 2412 11907
Size: 2412 9495
XbaI T'CTAG_A
Cuts at: 0 1618 2125 5121 7018 8772 10638 11907
Size: 1618 507 2996 1897 1754 1866 1269
Fragments arranged by size:
2996 1897 1866 1754 1618 1269 507
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4628 6064 8583 10807 10940 11907
Size: 4628 1436 2519 2224 133 967
Fragments arranged by size:
4628 2519 2224 1436 967 133
XmaI C'CCGG_G
Cuts at: 0 37 124 151 327 11907
Size: 37 87 27 176 11580
Fragments arranged by size:
11580 176 87 37 27
XmnI GAAnn'nnTTC
Cuts at: 0 1590 2178 5954 7931 11907
Size: 1590 588 3776 1977 3976
Fragments arranged by size:
3976 3776 1977 1590 588
ZraI GAC'GTC
Cuts at: 0 3448 11907
Size: 3448 8459
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SmaI SmlI SpeI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AclI AgeI AloI AscI AsiSI BsaAI BsiWI BsrGI
BssHII BtgZI ClaI FspI FspAI NruI PacI PmeI
PmlI PpiI PspXI RsrII SfiI SgfI SgrAI SnaBI
SphI SwaI XhoI