Restriction map for pF1KE1498
##################################
# NO CUT ENZYMES (   57 enzymes) #
##################################

AatII       AccIII      AflII       AscI        AseI        
AsnI        AvrII       BbrPI       BcgI        BfrI        
BlnI        BsaBI       BseAI       BsiWI       BspDI       
BspEI       BspHI       BsrDI       BssHII      Bst1107I    
BstEII      BstPI       BstZ17I     ClaI        CpoI        
EagI        EclXI       Eco52I      EcoO65I     EcoRV       
EcoT22I     HpaI        MamI        MfeI        MroI        
MunI        NdeI        NheI        NotI        NsiI        
PacI        PmaCI       PmeI        PmlI        PshBI       
PvuI        RcaI        RsrII       ScaI        SexAI       
SgrAI       SmaI        SnaBI       SpeI        SwaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AclI         2242  AACGTT               AA^CGTT
AgeI         2641  ACCGGT               A^CCGGT
AocI         3061  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      4014  AGCGCT               AGC^GCT
ApaI          466  GGGCCC               GGGCC^C
AspI         1534  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        3575  GGATCC               G^GATCC
BbeI         1312  GGCGCC               GGCGC^C
BciVI        2097  GGATAC               GTATCC(6/5)
BlpI          666  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      666  GCTCAGC              GC^T[ACGT]AGC
BsaWI        2641  ACCGGT               [AT]^CCGG[AT]
Bsp1407I     2495  TGTACA               T^GTACA
BspLU11I       21  ACATGT               A^CATGT
BsrGI        2495  TGTACA               T^GTACA
BstBI        2609  TTCGAA               TT^CGAA
Bsu36I       3061  CCTCAGG              CC^T[ACGT]AGG
CelII         666  GCTCAGC              GC^T[ACGT]AGC
DraI         3174  TTTAAA               TTT^AAA
DraIII       2824  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     4014  AGCGCT               AGC^GCT
Eco81I       3061  CCTCAGG              CC^T[ACGT]AGG
FseI         4286  GGCCGGCC             GGCCGG^CC
HindIII      3340  AAGCTT               A^AGCTT
KasI         1312  GGCGCC               G^GCGCC
KspI          658  CCGCGG               CCGC^GG
MluI         4637  ACGCGT               A^CGCGT
NarI         1312  GGCGCC               GG^CGCC
NcoI         1949  CCATGG               C^CATGG
NruI         1649  TCGCGA               TCG^CGA
NspV         2609  TTCGAA               TT^CGAA
PaeR7I       1831  CTCGAG               C^TCGAG
PflFI        1534  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        2641  ACCGGT               A^CCGGT
Psp1406I     2242  AACGTT               AA^CGTT
SacII         658  CCGCGG               CCGC^GG
SalI         1931  GTCGAC               G^TCGAC
SapI          944  GAAGAGC              GCTCTTC(1/4)
SbfI         4569  CCTGCAGG             CCTGCA^GG
SfiI         4281  GGCCAGGCCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1312  GGCGCC               GGC^GCC
SfuI         2609  TTCGAA               TT^CGAA
Sse8387I     4569  CCTGCAGG             CCTGCA^GG
SspBI        2495  TGTACA               T^GTACA
Tth111I      1534  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3303  TCTAGA               T^CTAGA
XcmI         3711  CCATGAAGAGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1831  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

Acc65I        238  GGTACC               G^GTACC
             3324  GGTACC              
AccI          334  GTAGAC               GT^[AC][GT]AC
             1931  GTCGAC              
AcyI         1312  GGCGCC               G[GA]^CG[CT]C
             2710  GACGCC              
AflIII         21  ACATGT               A^C[GA][CT]GT
             4637  ACGCGT              
AhdI         1030  GACCTCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1438  GACGGCAGGTC         
Alw44I       1339  GTGCAC               G^TGCAC
             4268  GTGCAC              
ApaLI        1339  GTGCAC               G^TGCAC
             4268  GTGCAC              
Asp700I      1964  GAATCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3217  GAAACTTTTC          
Asp718I       238  GGTACC               G^GTACC
             3324  GGTACC              
AspEI        1030  GACCTCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1438  GACGGCAGGTC         
AviII        1301  TGCGCA               TGC^GCA
             3122  TGCGCA              
BalI         1946  TGGCCA               TGG^CCA
             3656  TGGCCA              
BclI         2976  TGATCA               T^GATCA
             3354  TGATCA              
BglII        1808  AGATCT               A^GATCT
             3780  AGATCT              
BsaAI        2620  TACGTG               [CT]AC^GT[GA]
             3163  TACGTG              
BsaHI        1312  GGCGCC               G[GA]^CG[CT]C
             2710  GACGCC              
BsiEI        1646  CGGTCG               CG[GA][CT]^CG
             2336  CGGTCG              
BssSI          31  CACGAG               CACGAG(-5/-1)
             2281  CACGAG              
DrdI          313  GACTCTTCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2269  GACATTTGGGTC        
Eam1105I     1030  GACCTCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1438  GACGGCAGGTC         
EcoNI        1200  CCTGGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4246  CCTTTTGGAGG         
EcoRI         544  GAATTC               G^AATTC
             3289  GAATTC              
FbaI         2976  TGATCA               T^GATCA
             3354  TGATCA              
FspI         1301  TGCGCA               TGC^GCA
             3122  TGCGCA              
HaeII        1312  GGCGCC               [GA]GCGC^[CT]
             4014  AGCGCT              
Hin1I        1312  GGCGCC               G[GA]^CG[CT]C
             2710  GACGCC              
HincII         17  GTCAAC               GT[CT]^[GA]AC
             1931  GTCGAC              
HindII         17  GTCAAC               GT[CT]^[GA]AC
             1931  GTCGAC              
KpnI          238  GGTACC               GGTAC^C
             3324  GGTACC              
MluNI        1946  TGGCCA               TGG^CCA
             3656  TGGCCA              
MscI         1946  TGGCCA               TGG^CCA
             3656  TGGCCA              
PflMI         195  CCAGTCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2823  CCACCGAGTGG         
PshAI         296  GACCTGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2334  GACGGTCGTC          
SacI         1251  GAGCTC               GAGCT^C
             3139  GAGCTC              
SspI         1797  AATATT               AAT^ATT
             2187  AATATT              
StuI         2661  AGGCCT               AGG^CCT
             4692  AGGCCT              
Van91I        195  CCAGTCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2823  CCACCGAGTGG         
XmnI         1964  GAATCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3217  GAAACTTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaI        
AvaII       BanI        BanII       BbsI        BbvCI       
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SphI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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