################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## Acc65I AccI AclI AflII AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp718I AspEI AviII BbvCI BfrI BglII BsiWI BsmI BspDI Bst1107I BstAPI BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco81I EcoRV EcoT22I FseI FspI HincII HindII HpaI KpnI KspI MfeI MluI MseI MunI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I RsrII SacII SalI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspI StuI SwaI Tru9I XbaI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 1939 GACGTC GACGT^C AccIII 1943 TCCGGA T^CCGGA AcsI 2392 GAATTC [GA]^AATT[CT] AflIII 362 ACATGT A^C[GA][CT]GT ApoI 2392 GAATTC [GA]^AATT[CT] Asp700I 2115 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 2836 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2445 CCTAGG C^CTAGG BamHI 2631 GGATCC G^GATCC BbrPI 2913 CACGTG CAC^GTG BclI 3023 TGATCA T^GATCA BlnI 2445 CCTAGG C^CTAGG BsaBI 315 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1943 TCCGGA T^CCGGA Bsp1407I 1196 TGTACA T^GTACA BspEI 1943 TCCGGA T^CCGGA BspHI 1034 TCATGA T^CATGA BspLU11I 362 ACATGT A^CATGT BsrGI 1196 TGTACA T^GTACA BssHII 20 GCGCGC G^CGCGC BstEII 2172 GGTGACC G^GT[ACGT]ACC BstPI 2172 GGTGACC G^GT[ACGT]ACC BstYI 2631 GGATCC [GA]^GATC[CT] EcoNI 1788 CCTCAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2172 GGTGACC G^GT[ACGT]ACC EcoRI 2392 GAATTC G^AATTC FbaI 3023 TGATCA T^GATCA HindIII 2316 AAGCTT A^AGCTT MamI 315 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 2631 GGATCC [GA]^GATC[CT] MroI 1943 TCCGGA T^CCGGA NdeI 829 CATATG CA^TATG PflFI 2836 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2913 CACGTG CAC^GTG PmlI 2913 CACGTG CAC^GTG PshAI 2436 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 1743 CGATCG CGAT^CG RcaI 1034 TCATGA T^CATGA SapI 3111 GAAGAGC GCTCTTC(1/4) SbfI 397 CCTGCAGG CCTGCA^GG SmlI 2383 CTCAAG C^T[CT][GA]AG Sse8387I 397 CCTGCAGG CCTGCA^GG SspBI 1196 TGTACA T^GTACA Tth111I 2836 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 2888 CCACCATCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 2631 GGATCC [GA]^GATC[CT] XmnI 2115 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Aor51HI 197 AGCGCT AGC^GCT 1991 AGCGCT AspHI 229 GAGCTC G[AT]GC[AT]^C 1169 GAGCTC BcgI 146 GCAGCCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2807 GCACCTTCATCG BciVI 105 GGATAC GTATCC(6/5) 2493 GTATCC BsaAI 487 TACGTG [CT]AC^GT[GA] 2913 CACGTG BsaWI 257 ACCGGA [AT]^CCGG[AT] 1943 TCCGGA BsiHKAI 229 GAGCTC G[AT]GC[AT]^C 1169 GAGCTC BsrBI 187 GAGCGG CCGCTC(-3/-3) 310 GAGCGG BssSI 2011 CACGAG CACGAG(-5/-1) 2792 CACGAG DraIII 1366 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG 2728 CACTGTGTG EarI 151 CTCTTC CTCTTC(1/4) 3111 GAAGAG Eco47III 197 AGCGCT AGC^GCT 1991 AGCGCT Ksp632I 151 CTCTTC CTCTTC(1/4) 3111 GAAGAG NcoI 1251 CCATGG C^CATGG 2514 CCATGG NspI 362 ACATGT [GA]CATG^[CT] 584 GCATGT PflMI 1650 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2133 CCAGAGGATGG PstI 398 CTGCAG CTGCA^G 2206 CTGCAG SacI 229 GAGCTC GAGCT^C 1169 GAGCTC TfiI 113 GATTC G^A[AT]TC 678 GATTC Tsp509I 1949 AATT ^AATT 2393 AATT Van91I 1650 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2133 CCAGAGGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI StyI TaqI TseI Tsp45I TspRI TthHB8I XmaCI XmaI