(Circular) (Six-base) MAPSORT of: pF1KE2317.seq Check: 6858 from: 1 to: 6266 pF1KE2317 6266 bp With 182 enzymes: SgfI * January 12, 2011 16:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 984 1002 984 Size: 18 6248 AatII G_ACGT'C Cuts at: 2051 2051 Size: 6266 AccI GT'mk_AC Cuts at: 3304 3304 Size: 6266 Acc65I G'GTAC_C Cuts at: 3282 3282 Size: 6266 AclI AA'CG_TT Cuts at: 6106 6106 Size: 6266 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2287 4091 4523 5270 5538 2287 Size: 1804 432 747 268 3015 Fragments arranged by size: 3015 1804 747 432 268 AfeI AGC'GCT Cuts at: 307 2101 307 Size: 1794 4472 AflIII A'CryG_T Cuts at: 470 3618 4722 5699 470 Size: 3148 1104 977 1037 Fragments arranged by size: 3148 1104 1037 977 AgeI A'CCGG_T Cuts at: 4654 4654 Size: 6266 AleI CACnn'nnGTG Cuts at: 544 910 2137 2884 2904 544 Size: 366 1227 747 20 3906 Fragments arranged by size: 3906 1227 747 366 20 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2611 2611 Size: 6266 AlwNI CAG_nnn'CTG Cuts at: 549 999 1764 1914 2022 3571 5138 549 Size: 450 765 150 108 1549 1567 1677 Fragments arranged by size: 1677 1567 1549 765 450 150 108 ApaI G_GGCC'C Cuts at: 115 505 1494 2515 2761 115 Size: 390 989 1021 246 3620 Fragments arranged by size: 3620 1021 989 390 246 ApaLI G'TGCA_C Cuts at: 5036 5036 Size: 6266 ApoI r'AATT_y Cuts at: 2500 3270 5619 5875 2500 Size: 770 2349 256 2891 Fragments arranged by size: 2891 2349 770 256 AseI AT'TA_AT Cuts at: 20 20 Size: 6266 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6266 AvaI C'yCGr_G Cuts at: 249 332 1744 2179 3286 249 Size: 83 1412 435 1107 3229 Fragments arranged by size: 3229 1412 1107 435 83 AvrII C'CTAG_G Cuts at: 2553 2553 Size: 6266 BamHI G'GATC_C Cuts at: 2739 3291 2739 Size: 552 5714 BanI G'GyrC_C Cuts at: 325 706 745 754 919 929 1018 1087 1126 Size: 381 39 9 165 10 89 69 39 Cuts at: 1126 1527 2142 3282 3696 3944 3979 325 Size: 401 615 1140 414 248 35 2612 Fragments arranged by size: 2612 1140 615 414 401 381 248 165 89 69 39 39 35 10 9 BanII G_rGCy'C Cuts at: 115 341 505 1009 1281 1334 1494 2515 2761 Size: 226 164 504 272 53 160 1021 246 Cuts at: 2761 3174 3280 4310 115 Size: 413 106 1030 2071 Fragments arranged by size: 2071 1030 1021 504 413 272 246 226 164 160 106 53 BbeI G_GCGC'C Cuts at: 749 1022 1091 3700 3948 749 Size: 273 69 2609 248 3067 Fragments arranged by size: 3067 2609 273 248 69 BbsI GAAGACnn'nnnn_ Cuts at: 572 1131 1713 572 Size: 559 582 5125 Fragments arranged by size: 5125 582 559 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 243 277 2904 2938 243 Size: 34 2627 34 3571 Fragments arranged by size: 3571 2627 34 34 BciVI GTATCCnnnnn_n' Cuts at: 207 2612 4164 4925 6266 207 Size: 2405 1552 761 1341 207 Fragments arranged by size: 2405 1552 1341 761 207 BclI T'GATC_A Cuts at: 3131 3786 3131 Size: 655 5611 BglI GCCn_nnn'nGGC Cuts at: 263 509 704 1202 1689 2122 263 Size: 246 195 498 487 433 4407 Fragments arranged by size: 4407 498 487 433 246 195 BglII A'GATC_T Cuts at: 3781 5818 3781 Size: 2037 4229 BlpI GC'TnA_GC Cuts at: 958 2023 2398 3378 958 Size: 1065 375 980 3846 Fragments arranged by size: 3846 1065 980 375 Bme1580I G_kGCm'C Cuts at: 115 330 350 391 505 531 709 924 1494 Size: 215 20 41 114 26 178 215 570 Cuts at: 1494 1532 2515 2761 2835 2857 3891 3984 5040 Size: 38 983 246 74 22 1034 93 1056 Cuts at: 5040 115 Size: 1341 Fragments arranged by size: 1341 1056 1034 983 570 246 215 215 178 114 93 74 41 38 26 22 20 BmgBI CAC'GTC Cuts at: 2327 2327 Size: 6266 BmrI ACTGGGnnnn_n' Cuts at: 1193 3478 3559 3894 6038 1193 Size: 2285 81 335 2144 1421 Fragments arranged by size: 2285 2144 1421 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 639 4633 639 Size: 3994 2272 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 609 703 1021 1681 1786 1936 2017 2371 2581 Size: 94 318 660 105 150 81 354 210 Cuts at: 2581 2675 609 Size: 94 4200 Fragments arranged by size: 4200 660 354 318 210 150 105 94 94 81 Bpu10I CC'TnA_GC Cuts at: 2065 2065 Size: 6266 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2476 3442 4321 4813 5111 2476 Size: 966 879 492 298 3631 Fragments arranged by size: 3631 966 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 971 2194 2651 5970 971 Size: 1223 457 3319 1267 Fragments arranged by size: 3319 1267 1223 457 BsaAI yAC'GTr Cuts at: 597 3023 3619 597 Size: 2426 596 3244 Fragments arranged by size: 3244 2426 596 BsaBI GATnn'nnATC Cuts at: 6 427 3780 5817 6 Size: 421 3353 2037 455 Fragments arranged by size: 3353 2037 455 421 BsaHI Gr'CG_yC Cuts at: 746 1019 1088 1883 2048 2114 3697 3945 4647 Size: 273 69 795 165 66 1583 248 702 Cuts at: 4647 6143 746 Size: 1496 869 Fragments arranged by size: 1583 1496 869 795 702 273 248 165 69 66 BsaWI w'CCGG_w Cuts at: 365 2051 3455 3976 4654 4928 5075 5486 365 Size: 1686 1404 521 678 274 147 411 1145 Fragments arranged by size: 1686 1404 1145 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 473 503 473 Size: 30 6236 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1002 1324 3073 1002 Size: 322 1749 4195 Fragments arranged by size: 4195 1749 322 BseYI C'CCAG_C Cuts at: 121 702 1346 1371 1863 2217 2963 3123 3691 Size: 581 644 25 492 354 746 160 568 Cuts at: 3691 4449 5026 5522 121 Size: 758 577 496 865 Fragments arranged by size: 865 758 746 644 581 577 568 496 492 354 160 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 874 1538 2272 2329 3128 874 Size: 664 734 57 799 4012 Fragments arranged by size: 4012 799 734 664 57 BsiEI CG_ry'CG Cuts at: 104 182 275 1684 1854 3071 3808 3854 5062 Size: 78 93 1409 170 1217 737 46 1208 Cuts at: 5062 104 Size: 1308 Fragments arranged by size: 1409 1308 1217 1208 737 170 93 78 46 BsiHKAI G_wGCw'C Cuts at: 341 1281 3280 4058 4248 5040 341 Size: 940 1999 778 190 792 1567 Fragments arranged by size: 1999 1567 940 792 778 190 BsmBI CGTCTCn'nnnn_ Cuts at: 201 679 2039 201 Size: 478 1360 4428 Fragments arranged by size: 4428 1360 478 Bsp1286I G_dGCh'C Cuts at: 115 330 341 350 391 505 531 709 924 Size: 215 11 9 41 114 26 178 215 Cuts at: 924 1009 1281 1334 1494 1532 2515 2761 2835 Size: 85 272 53 160 38 983 246 74 Cuts at: 2835 2857 3174 3280 3891 3984 4058 4248 4310 Size: 22 317 106 611 93 74 190 62 Cuts at: 4310 5040 115 Size: 730 1341 Fragments arranged by size: 1341 983 730 611 317 272 246 215 215 190 178 160 114 106 93 85 74 74 62 53 41 38 26 22 11 9 BspEI T'CCGG_A Cuts at: 2051 5486 2051 Size: 3435 2831 BspHI T'CATG_A Cuts at: 1142 5442 1142 Size: 4300 1966 BspMI ACCTGCnnnn'nnnn_ Cuts at: 320 861 984 989 1002 1341 3316 3530 3832 Size: 541 123 5 13 339 1975 214 302 Cuts at: 3832 4213 320 Size: 381 2373 Fragments arranged by size: 2373 1975 541 381 339 302 214 123 13 5 BsrBI CCG'CTC Cuts at: 50 297 420 4558 4612 6096 6261 50 Size: 247 123 4138 54 1484 165 55 Fragments arranged by size: 4138 1484 247 165 123 55 54 BsrDI GCAATG_nn' Cuts at: 612 1037 2589 2634 4178 612 Size: 425 1552 45 1544 2700 Fragments arranged by size: 2700 1552 1544 425 45 BsrFI r'CCGG_y Cuts at: 181 1679 1702 2414 3066 4264 4445 4654 181 Size: 1498 23 712 652 1198 181 209 1793 Fragments arranged by size: 1793 1498 1198 712 652 209 181 23 BsrGI T'GTAC_A Cuts at: 1304 1304 Size: 6266 BssHII G'CGCG_C Cuts at: 128 128 Size: 6266 BssSI C'ACGA_G Cuts at: 2119 2900 4537 4895 2119 Size: 781 1637 358 3490 Fragments arranged by size: 3490 1637 781 358 BstAPI GCAn_nnn'nTGC Cuts at: 3643 3643 Size: 6266 BstBI TT'CG_AA Cuts at: 4627 4627 Size: 6266 BstEII G'GTnAC_C Cuts at: 2280 2280 Size: 6266 BstXI CCAn_nnnn'nTGG Cuts at: 1044 2629 3006 1044 Size: 1585 377 4304 Fragments arranged by size: 4304 1585 377 BstYI r'GATC_y Cuts at: 1 2739 3291 3758 3781 4116 4362 5374 5460 Size: 2738 552 467 23 335 246 1012 86 Cuts at: 5460 5472 5818 1 Size: 12 346 449 Fragments arranged by size: 2738 1012 552 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 925 1359 1725 2606 2622 3101 107 Size: 818 434 366 881 16 479 3272 Fragments arranged by size: 3272 881 818 479 434 366 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 4216 4397 4560 4216 Size: 181 163 5922 Fragments arranged by size: 5922 181 163 BtsI GCAGTG_nn' Cuts at: 395 1713 3509 395 Size: 1318 1796 3152 Fragments arranged by size: 3152 1796 1318 DraI TTT'AAA Cuts at: 3265 3265 Size: 6266 DraIII CAC_nnn'GTG Cuts at: 1479 2841 1479 Size: 1362 4904 DrdI GACnn_nn'nnGTC Cuts at: 3972 4830 3972 Size: 858 5408 EaeI y'GGCC_r Cuts at: 179 450 491 1226 1388 1565 1681 3068 3098 Size: 271 41 735 162 177 116 1387 30 Cuts at: 3098 3851 4025 4416 4443 5788 179 Size: 753 174 391 27 1345 657 Fragments arranged by size: 1387 1345 753 735 657 391 271 177 174 162 116 41 30 27 EagI C'GGCC_G Cuts at: 179 1681 3068 3851 179 Size: 1502 1387 783 2594 Fragments arranged by size: 2594 1502 1387 783 EarI CTCTTCn'nnn_ Cuts at: 265 3214 4289 4499 265 Size: 2949 1075 210 2032 Fragments arranged by size: 2949 2032 1075 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 189 989 1792 3226 4474 4784 4930 6076 189 Size: 800 803 1434 1248 310 146 1146 379 Fragments arranged by size: 1434 1248 1146 803 800 379 310 146 EcoICRI GAG'CTC Cuts at: 339 1279 3278 339 Size: 940 1999 3327 Fragments arranged by size: 3327 1999 940 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 609 703 1021 1681 1786 1936 2017 2287 2371 Size: 94 318 660 105 150 81 270 84 Cuts at: 2371 2581 2675 4091 4523 5270 5538 609 Size: 210 94 1416 432 747 268 1337 Fragments arranged by size: 1416 1337 747 660 432 318 270 268 210 150 105 94 94 84 81 EcoNI CCTnn'n_nnAGG Cuts at: 1900 1900 Size: 6266 EcoO109I rG'GnC_Cy Cuts at: 112 501 502 828 1074 2183 2298 2543 2633 Size: 389 1 326 246 1109 115 245 90 Cuts at: 2633 2757 2758 3405 112 Size: 124 1 647 2973 Fragments arranged by size: 2973 1109 647 389 326 246 245 124 115 90 1 1 EcoRI G'AATT_C Cuts at: 2500 3270 2500 Size: 770 5496 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3174 4285 3174 Size: 1111 5155 FspI TGC'GCA Cuts at: 4047 4047 Size: 6266 HaeII r_GCGC'y Cuts at: 309 749 1022 1037 1091 2103 2128 3700 3948 Size: 440 273 15 54 1012 25 1572 248 Cuts at: 3948 4970 5949 309 Size: 1022 979 626 Fragments arranged by size: 1572 1022 1012 979 626 440 273 248 54 25 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 506 538 1836 1868 2349 2381 2648 2680 2692 Size: 32 1298 32 481 32 267 32 12 Cuts at: 2692 2724 3028 3060 4108 4138 4140 4170 506 Size: 32 304 32 1048 30 2 30 2602 Fragments arranged by size: 2602 1298 1048 481 304 267 32 32 32 32 32 32 30 30 12 2 HincII GTy'rAC Cuts at: 3305 3305 Size: 6266 HindIII A'AGCT_T Cuts at: 2424 2424 Size: 6266 KasI G'GCGC_C Cuts at: 745 1018 1087 3696 3944 745 Size: 273 69 2609 248 3067 Fragments arranged by size: 3067 2609 273 248 69 KpnI G_GTAC'C Cuts at: 3286 3286 Size: 6266 MluI A'CGCG_T Cuts at: 5699 5699 Size: 6266 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 4937 5121 4937 Size: 184 6082 MscI TGG'CCA Cuts at: 452 493 1228 1390 4027 452 Size: 41 735 162 2637 2691 Fragments arranged by size: 2691 2637 735 162 41 MslI CAynn'nnrTG Cuts at: 407 433 544 910 1819 2074 2137 2278 2627 Size: 26 111 366 909 255 63 141 349 Cuts at: 2627 2884 2904 3004 4255 4382 407 Size: 257 20 100 1251 127 2291 Fragments arranged by size: 2291 1251 909 366 349 257 255 141 127 111 100 63 26 20 MspA1I CmG'CkG Cuts at: 573 702 713 1540 1717 2313 2736 3377 3691 Size: 129 11 827 177 596 423 641 314 Cuts at: 3691 4051 5064 5309 573 Size: 360 1013 245 1530 Fragments arranged by size: 1530 1013 827 641 596 423 360 314 245 177 129 11 NaeI GCC'GGC Cuts at: 183 1681 1704 3068 4447 183 Size: 1498 23 1364 1379 2002 Fragments arranged by size: 2002 1498 1379 1364 23 NarI GG'CG_CC Cuts at: 746 1019 1088 3697 3945 746 Size: 273 69 2609 248 3067 Fragments arranged by size: 3067 2609 273 248 69 NcoI C'CATG_G Cuts at: 107 1359 2622 107 Size: 1252 1263 3751 Fragments arranged by size: 3751 1263 1252 NdeI CA'TA_TG Cuts at: 938 938 Size: 6266 NgoMIV G'CCGG_C Cuts at: 181 1679 1702 3066 4445 181 Size: 1498 23 1364 1379 2002 Fragments arranged by size: 2002 1498 1379 1364 23 NspI r_CATG'y Cuts at: 474 696 3319 3819 4726 474 Size: 222 2623 500 907 2014 Fragments arranged by size: 2623 2014 907 500 222 PasI CC'CwG_GG Cuts at: 2695 2695 Size: 6266 PciI A'CATG_T Cuts at: 470 4722 470 Size: 4252 2014 PflMI CCAn_nnn'nTGG Cuts at: 1764 2247 1764 Size: 483 5783 PmeI GTTT'AAAC Cuts at: 3265 3265 Size: 6266 PmlI CAC'GTG Cuts at: 3023 3023 Size: 6266 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1921 1953 5405 5437 1921 Size: 32 3452 32 2750 Fragments arranged by size: 3452 2750 32 32 PpuMI rG'GwC_Cy Cuts at: 828 1074 2298 2543 2633 828 Size: 246 1224 245 90 4461 Fragments arranged by size: 4461 1224 246 245 90 PshAI GACnn'nnGTC Cuts at: 2548 2548 Size: 6266 PspOMI G'GGCC_C Cuts at: 111 501 1490 2511 2757 111 Size: 390 989 1021 246 3620 Fragments arranged by size: 3620 1021 989 390 246 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 721 753 1612 1644 721 Size: 32 859 32 5343 Fragments arranged by size: 5343 859 32 32 PstI C_TGCA'G Cuts at: 510 2318 3313 3998 510 Size: 1808 995 685 2778 Fragments arranged by size: 2778 1808 995 685 PvuI CG_AT'CG Cuts at: 104 1854 104 Size: 1750 4516 PvuII CAG'CTG Cuts at: 702 1540 2313 2736 3691 4051 702 Size: 838 773 423 955 360 2917 Fragments arranged by size: 2917 955 838 773 423 360 RsrII CG'GwC_CG Cuts at: 4461 4461 Size: 6266 SacI G_AGCT'C Cuts at: 341 1281 3280 341 Size: 940 1999 3327 Fragments arranged by size: 3327 1999 940 SalI G'TCGA_C Cuts at: 3303 3303 Size: 6266 SanDI GG'GwC_CC Cuts at: 2543 2543 Size: 6266 SapI GCTCTTCn'nnn_ Cuts at: 3214 4289 4499 3214 Size: 1075 210 4981 Fragments arranged by size: 4981 1075 210 SbfI CC_TGCA'GG Cuts at: 510 3313 510 Size: 2803 3463 ScaI AGT'ACT Cuts at: 5783 5783 Size: 6266 SfcI C'TryA_G Cuts at: 33 506 1629 2314 3309 3994 4987 5178 33 Size: 473 1123 685 995 685 993 191 1121 Fragments arranged by size: 1123 1121 995 993 685 685 473 191 SfoI GGC'GCC Cuts at: 747 1020 1089 3698 3946 747 Size: 273 69 2609 248 3067 Fragments arranged by size: 3067 2609 273 248 69 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6266 SmaI CCC'GGG Cuts at: 251 334 2181 3288 251 Size: 83 1847 1107 3229 Fragments arranged by size: 3229 1847 1107 83 SmlI C'TyrA_G Cuts at: 2491 3421 4336 4828 5090 2491 Size: 930 915 492 262 3667 Fragments arranged by size: 3667 930 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 6266 SphI G_CATG'C Cuts at: 3319 3819 3319 Size: 500 5766 StyI C'CwwG_G Cuts at: 107 840 1359 1523 2553 2622 3400 3754 107 Size: 733 519 164 1030 69 778 354 2619 Fragments arranged by size: 2619 1030 778 733 519 354 164 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 261 556 739 1055 2437 2960 3094 3265 3849 Size: 295 183 316 1382 523 134 171 584 Cuts at: 3849 3982 4441 4650 261 Size: 133 459 209 1877 Fragments arranged by size: 1877 1382 584 523 459 316 295 209 183 171 134 133 TatI w'GTAC_w Cuts at: 737 1304 2036 5781 737 Size: 567 732 3745 1222 Fragments arranged by size: 3745 1222 732 567 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 473 505 2332 2364 5783 5815 473 Size: 32 1827 32 3419 32 924 Fragments arranged by size: 3419 1827 924 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2947 4063 2947 Size: 1116 5150 XbaI T'CTAG_A Cuts at: 3297 3297 Size: 6266 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3003 3761 3003 Size: 758 5508 XmaI C'CCGG_G Cuts at: 249 332 2179 3286 249 Size: 83 1847 1107 3229 Fragments arranged by size: 3229 1847 1107 83 XmnI GAAnn'nnTTC Cuts at: 2227 2227 Size: 6266 ZraI GAC'GTC Cuts at: 2049 2049 Size: 6266 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: AflII AhdI AloI AscI BaeI BbvCI BfrBI BmtI BsiWI BsmI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HpaI MfeI NheI NotI NruI NsiI PacI PfoI PsiI PspXI SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI