Restriction map for
pF1KB6675
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BssHII Bst1107I
BstBI BstZ17I CelII ClaI CpoI
DraI DrdI Eam1105I EarI Eco47III
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KpnI
Ksp632I KspI MaeI MamI MboII
MfeI MluI MluNI MroI MscI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AfaI 108 GTAC GT^AC
AlwI 570 GATCC GGATC(4/5)
AlwNI 934 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 643 CCTGAGG CC^T[ACGT]AGG
BbeI 288 GGCGCC GGCGC^C
BbrPI 478 CACGTG CAC^GTG
BcnI 1275 CCCGG CC^[GC]GG
BsmAI 349 GAGAC GTCTC(1/5)
BsmBI 349 GAGACG CGTCTC(1/5)
Bsp1407I 107 TGTACA T^GTACA
BsrGI 107 TGTACA T^GTACA
BstYI 569 AGATCC [GA]^GATC[CT]
Bsu36I 643 CCTGAGG CC^T[ACGT]AGG
DdeI 644 CTGAG C^T[ACGT]AG
DpnII 570 GATC ^GATC
DraII 980 AGGCCCT [GA]G^G[ACGT]CC[CT]
EagI 18 CGGCCG C^GGCCG
EclXI 18 CGGCCG C^GGCCG
Eco52I 18 CGGCCG C^GGCCG
Eco81I 643 CCTGAGG CC^T[ACGT]AGG
EcoO109I 980 AGGCCCT [GA]G^G[ACGT]CC[CT]
HindIII 1045 AAGCTT A^AGCTT
KasI 288 GGCGCC G^GCGCC
MboI 570 GATC ^GATC
MflI 569 AGATCC [GA]^GATC[CT]
NaeI 860 GCCGGC GCC^GGC
NarI 288 GGCGCC GG^CGCC
NciI 1275 CCCGG CC^[GC]GG
NcoI 26 CCATGG C^CATGG
NdeII 570 GATC ^GATC
NgoMIV 860 GCCGGC G^CCGGC
PflMI 717 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 478 CACGTG CAC^GTG
PmlI 478 CACGTG CAC^GTG
PvuII 828 CAGCTG CAG^CTG
RsaI 108 GTAC GT^AC
Sau3AI 570 GATC ^GATC
SfcI 1005 CTACAG C^T[GA][CT]AG
SfoI 288 GGCGCC GGC^GCC
SmlI 985 CTCAAG C^T[CT][GA]AG
SphI 91 GCATGC GCATG^C
SspBI 107 TGTACA T^GTACA
Van91I 717 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 903 CCACAGCGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 569 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Alw44I 255 GTGCAC G^TGCAC
537 GTGCAC
ApaLI 255 GTGCAC G^TGCAC
537 GTGCAC
AvaII 483 GGACC G^G[AT]CC
593 GGTCC
BpmI 274 CTCCAG CTGGAG(16/14)
653 CTCCAG
BsaAI 456 CACGTA [CT]AC^GT[GA]
478 CACGTG
BsgI 498 CTGCAC GTGCAG(16/14)
1251 GTGCAG
BsiEI 18 CGGCCG CG[GA][CT]^CG
236 CGACCG
BsmFI 1067 GGGAC GGGAC(10/14)
69 GTCCC
BspMI 7 GCAGGT ACCTGC(4/8)
685 GCAGGT
BsrFI 16 ACCGGC [GA]^CCGG[CT]
860 GCCGGC
BssSI 400 CTCGTG CACGAG(-5/-1)
1237 CTCGTG
BstEII 693 GGTGACC G^GT[ACGT]ACC
726 GGTGACC
BstPI 693 GGTGACC G^GT[ACGT]ACC
726 GGTGACC
Cfr10I 16 ACCGGC [GA]^CCGG[CT]
860 GCCGGC
EcoNI 144 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
812 CCTTCAAGAGG
EcoO65I 693 GGTGACC G^GT[ACGT]ACC
726 GGTGACC
NspI 91 GCATGC [GA]CATG^[CT]
281 GCATGT
SfaNI 917 GCATC GCATC(5/9)
1220 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaI BanI BanII BbvI BglI
BmyI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsoBI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DraIII
DsaI EaeI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NlaIII NlaIV PleI
Sau96I ScrFI StuI StyI TseI
Tsp45I TspRI