Restriction map for pF1KB6675
 (Circular) (Six-base) MAPSORT of: pF1KB6675.seq  Check: 487  from: 1  to: 4394



pF1KB6675  4394 bp

 With 182 enzymes: SgfI * 

                        March 22, 2013 15:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    106     784     106 
   Size:        678    3716
 
AccI GT'mk_AC

Cuts at:   1432    1432 
   Size:       4394
 
Acc65I G'GTAC_C

Cuts at:   1410    1410 
   Size:       4394
 
AclI AA'CG_TT

Cuts at:   4234    4234 
   Size:       4394
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2219    2651    3398    3666    2219 
   Size:        432     747     268    2947

  Fragments arranged by size: 

                2947     747     432     268
 
AflIII A'CryG_T

Cuts at:   1746    2850    3827    1746 
   Size:       1104     977    2313

  Fragments arranged by size: 

                2313    1104     977
 
AgeI A'CCGG_T

Cuts at:   2782    2782 
   Size:       4394
 
AleI CACnn'nnGTG

Cuts at:    227     227 
   Size:       4394
 
AlwNI CAG_nnn'CTG

Cuts at:   1047    1699    3266    1047 
   Size:        652    1567    2175

  Fragments arranged by size: 

                2175    1567     652
 
ApaLI G'TGCA_C

Cuts at:    363     645    3164     363 
   Size:        282    2519    1593

  Fragments arranged by size: 

                2519    1593     282
 
ApoI r'AATT_y

Cuts at:   1398    3747    4003    1398 
   Size:       2349     256    1789

  Fragments arranged by size: 

                2349    1789     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4394
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4394
 
AvaI C'yCGr_G

Cuts at:    501     839    1080    1216    1414     501 
   Size:        338     241     136     198    3481

  Fragments arranged by size: 

                3481     338     241     198     136
 
BamHI G'GATC_C

Cuts at:   1419    1419 
   Size:       4394
 
BanI G'GyrC_C

Cuts at:    118     187     396     756     844    1187    1410    1824    2072 
   Size:         69     209     360      88     343     223     414     248

Cuts at:   2072    2107     118 
   Size:         35    2405

  Fragments arranged by size: 

                2405     414     360     343     248     223     209      88
                  69      35
 
BanII G_rGCy'C

Cuts at:    305     383     502     721    1408    2438     305 
   Size:         78     119     219     687    1030    2261

  Fragments arranged by size: 

                2261    1030     687     219     119      78
 
BbeI G_GCGC'C

Cuts at:    400    1828    2076     400 
   Size:       1428     248    2718

  Fragments arranged by size: 

                2718    1428     248
 
BciVI GTATCCnnnnn_n'

Cuts at:   2292    3053    4394    2292 
   Size:        761    1341    2292

  Fragments arranged by size: 

                2292    1341     761
 
BclI T'GATC_A

Cuts at:   1914    1914 
   Size:       4394
 
BglI GCCn_nnn'nGGC

Cuts at:    112     196     209     560    1312     112 
   Size:         84      13     351     752    3194

  Fragments arranged by size: 

                3194     752     351      84      13
 
BglII A'GATC_T

Cuts at:   1909    3946    1909 
   Size:       2037    2357
 
BlpI GC'TnA_GC

Cuts at:   1506    1506 
   Size:       4394
 
Bme1580I G_kGCm'C

Cuts at:    367     556     649    2019    2112    3168     367 
   Size:        189      93    1370      93    1056    1593

  Fragments arranged by size: 

                1593    1370    1056     189      93      93
 
BmgBI CAC'GTC

Cuts at:    333     333 
   Size:       4394
 
BmrI ACTGGGnnnn_n'

Cuts at:    473    1606    1687    2022    4166     473 
   Size:       1133      81     335    2144     701

  Fragments arranged by size: 

                2144    1133     701     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    516    1024    1360    2761     516 
   Size:        508     336    1401    2149

  Fragments arranged by size: 

                2149    1401     508     336
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    367     746     367 
   Size:        379    4015
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1078    1570    2449    2941    3239    1078 
   Size:        492     879     492     298    2233

  Fragments arranged by size: 

                2233     879     492     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4098    4098 
   Size:       4394
 
BsaAI yAC'GTr

Cuts at:    566     588    1747     566 
   Size:         22    1159    3213

  Fragments arranged by size: 

                3213    1159      22
 
BsaBI GATnn'nnATC

Cuts at:      6    1908    3945       6 
   Size:       1902    2037     455

  Fragments arranged by size: 

                2037    1902     455
 
BsaHI Gr'CG_yC

Cuts at:    397     419     662     985    1304    1825    2073    2775    4271 
   Size:         22     243     323     319     521     248     702    1496

Cuts at:   4271     397 
   Size:        520

  Fragments arranged by size: 

                1496     702     521     520     323     319     248     243
                  22
 
BsaWI w'CCGG_w

Cuts at:   1583    2104    2782    3056    3203    3614    1583 
   Size:        521     678     274     147     411    2363

  Fragments arranged by size: 

                2363     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1213    1243    1342    1372    1213 
   Size:         30      99      30    4235

  Fragments arranged by size: 

                4235      99      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    562     688    1156     562 
   Size:        126     468    3800

  Fragments arranged by size: 

                3800     468     126
 
BseYI C'CCAG_C

Cuts at:    269     653     736     824    1128    1203    1274    1819    2577 
   Size:        384      83      88     304      75      71     545     758

Cuts at:   2577    3154    3650     269 
   Size:        577     496    1013

  Fragments arranged by size: 

                1013     758     577     545     496     384     304      88
                  83      75      71
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    591    1380     591 
   Size:        789    3605
 
BsiEI CG_ry'CG

Cuts at:    104     129     347    1936    1982    3190     104 
   Size:         25     218    1589      46    1208    1308

  Fragments arranged by size: 

                1589    1308    1208     218      46      25
 
BsiHKAI G_wGCw'C

Cuts at:    367     649    1223    1408    2186    2376    3168     367 
   Size:        282     574     185     778     190     792    1593

  Fragments arranged by size: 

                1593     792     778     574     282     190     185
 
BsmBI CGTCTCn'nnnn_

Cuts at:    451     451 
   Size:       4394
 
Bsp1286I G_dGCh'C

Cuts at:    305     367     383     502     556     649     721    1223    1408 
   Size:         62      16     119      54      93      72     502     185

Cuts at:   1408    2019    2112    2186    2376    2438    3168     305 
   Size:        611      93      74     190      62     730    1531

  Fragments arranged by size: 

                1531     730     611     502     190     185     119      93
                  93      74      72      62      62      54      16
 
BspEI T'CCGG_A

Cuts at:   3614    3614 
   Size:       4394
 
BspHI T'CATG_A

Cuts at:   3570    3570 
   Size:       4394
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    106     784    1444    1658    1960    2341     106 
   Size:        678     660     214     302     381    2159

  Fragments arranged by size: 

                2159     678     660     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2686    2740    4224    4389      50 
   Size:       2636      54    1484     165      55

  Fragments arranged by size: 

                2636    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2306    2306 
   Size:       4394
 
BsrFI r'CCGG_y

Cuts at:    124     968    2392    2573    2782     124 
   Size:        844    1424     181     209    1736

  Fragments arranged by size: 

                1736    1424     844     209     181
 
BsrGI T'GTAC_A

Cuts at:    215     215 
   Size:       4394
 
BssSI C'ACGA_G

Cuts at:    508    1345    2665    3023     508 
   Size:        837    1320     358    1879

  Fragments arranged by size: 

                1879    1320     837     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    245     362     371     614    1771     245 
   Size:        117       9     243    1157    2868

  Fragments arranged by size: 

                2868    1157     243     117       9
 
BstBI TT'CG_AA

Cuts at:   2755    2755 
   Size:       4394
 
BstEII G'GTnAC_C

Cuts at:    801     834     801 
   Size:         33    4361
 
BstXI CCAn_nnnn'nTGG

Cuts at:    277     588     789     813     277 
   Size:        311     201      24    3858

  Fragments arranged by size: 

                3858     311     201      24
 
BstYI r'GATC_y

Cuts at:      1     677    1419    1886    1909    2244    2490    3502    3588 
   Size:        676     742     467      23     335     246    1012      86

Cuts at:   3588    3600    3946       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     742     676     467     449     346     335     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    752     752 
   Size:       4394
 
BtgI C'CryG_G

Cuts at:    107     134     830    1031     107 
   Size:         27     696     201    3470

  Fragments arranged by size: 

                3470     696     201      27
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2344    2525    2688    2344 
   Size:        181     163    4050

  Fragments arranged by size: 

                4050     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1368    1370    1637    1368 
   Size:          2     267    4125

  Fragments arranged by size: 

                4125     267       2
 
DraI TTT'AAA

Cuts at:   1393    1393 
   Size:       4394
 
DraIII CAC_nnn'GTG

Cuts at:    371     542     614     371 
   Size:        171      72    4151

  Fragments arranged by size: 

                4151     171      72
 
DrdI GACnn_nn'nnGTC

Cuts at:   2100    2958    2100 
   Size:        858    3536
 
EaeI y'GGCC_r

Cuts at:    126     684     970    1313    1979    2153    2544    2571    3916 
   Size:        558     286     343     666     174     391      27    1345

Cuts at:   3916     126 
   Size:        604

  Fragments arranged by size: 

                1345     666     604     558     391     343     286     174
                  27
 
EagI C'GGCC_G

Cuts at:    126    1979     126 
   Size:       1853    2541
 
EarI CTCTTCn'nnn_

Cuts at:   2417    2627    2417 
   Size:        210    4184
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2602    2912    3058    4204    2602 
   Size:        310     146    1146    2792

  Fragments arranged by size: 

                2792    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1406    1406 
   Size:       4394
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    367     746    2219    2651    3398    3666     367 
   Size:        379    1473     432     747     268    1095

  Fragments arranged by size: 

                1473    1095     747     432     379     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    256     924     256 
   Size:        668    3726
 
EcoO109I rG'GnC_Cy

Cuts at:   1089    1533    1089 
   Size:        444    3950
 
EcoRI G'AATT_C

Cuts at:   1398    1398 
   Size:       4394
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2413    2413 
   Size:       4394
 
FspI TGC'GCA

Cuts at:   2175    2175 
   Size:       4394
 
HaeII r_GCGC'y

Cuts at:    400     471     633    1295    1828    2076    3098    4077     400 
   Size:         71     162     662     533     248    1022     979     717

  Fragments arranged by size: 

                1022     979     717     662     533     248     162      71
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2236    2266    2268    2298    2236 
   Size:         30       2      30    4332

  Fragments arranged by size: 

                4332      30      30       2
 
HincII GTy'rAC

Cuts at:   1433    1433 
   Size:       4394
 
HindIII A'AGCT_T

Cuts at:   1153    1153 
   Size:       4394
 
KasI G'GCGC_C

Cuts at:    396    1824    2072     396 
   Size:       1428     248    2718

  Fragments arranged by size: 

                2718    1428     248
 
KpnI G_GTAC'C

Cuts at:   1414    1414 
   Size:       4394
 
MluI A'CGCG_T

Cuts at:   3827    3827 
   Size:       4394
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    353     596     670    3065    3249     353 
   Size:        243      74    2395     184    1498

  Fragments arranged by size: 

                2395    1498     243     184      74
 
MscI TGG'CCA

Cuts at:   2155    2155 
   Size:       4394
 
MslI CAynn'nnrTG

Cuts at:    227     811    1030    2383    2510     227 
   Size:        584     219    1353     127    2111

  Fragments arranged by size: 

                2111    1353     584     219     127
 
MspA1I CmG'CkG

Cuts at:    227     431     938    1505    1819    2179    3192    3437     227 
   Size:        204     507     567     314     360    1013     245    1184

  Fragments arranged by size: 

                1184    1013     567     507     360     314     245     204
            
 
NaeI GCC'GGC

Cuts at:    970    2575     970 
   Size:       1605    2789
 
NarI GG'CG_CC

Cuts at:    397    1825    2073     397 
   Size:       1428     248    2718

  Fragments arranged by size: 

                2718    1428     248
 
NcoI C'CATG_G

Cuts at:    107     134     107 
   Size:         27    4367
 
NgoMIV G'CCGG_C

Cuts at:    968    2573     968 
   Size:       1605    2789
 
NspI r_CATG'y

Cuts at:    203     393    1447    1947    2854     203 
   Size:        190    1054     500     907    1743

  Fragments arranged by size: 

                1743    1054     907     500     190
 
PciI A'CATG_T

Cuts at:   2850    2850 
   Size:       4394
 
PflMI CCAn_nnn'nTGG

Cuts at:    831     831 
   Size:       4394
 
PmeI GTTT'AAAC

Cuts at:   1393    1393 
   Size:       4394
 
PmlI CAC'GTG

Cuts at:    588     588 
   Size:       4394
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    758     790    3533    3565     758 
   Size:         32    2743      32    1587

  Fragments arranged by size: 

                2743    1587      32      32
 
PstI C_TGCA'G

Cuts at:   1441    2126    1441 
   Size:        685    3709
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4394
 
PvuII CAG'CTG

Cuts at:    938    1819    2179     938 
   Size:        881     360    3153

  Fragments arranged by size: 

                3153     881     360
 
RsrII CG'GwC_CG

Cuts at:   2589    2589 
   Size:       4394
 
SacI G_AGCT'C

Cuts at:   1408    1408 
   Size:       4394
 
SalI G'TCGA_C

Cuts at:   1431    1431 
   Size:       4394
 
SapI GCTCTTCn'nnn_

Cuts at:   2417    2627    2417 
   Size:        210    4184
 
SbfI CC_TGCA'GG

Cuts at:   1441    1441 
   Size:       4394
 
ScaI AGT'ACT

Cuts at:   3911    3911 
   Size:       4394
 
SfcI C'TryA_G

Cuts at:     33    1113    1437    2122    3115    3306      33 
   Size:       1080     324     685     993     191    1121

  Fragments arranged by size: 

                1121    1080     993     685     324     191
 
SfoI GGC'GCC

Cuts at:    398    1826    2074     398 
   Size:       1428     248    2718

  Fragments arranged by size: 

                2718    1428     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4394
 
SmaI CCC'GGG

Cuts at:   1416    1416 
   Size:       4394
 
SmlI C'TyrA_G

Cuts at:   1093    1549    2464    2956    3218    1093 
   Size:        456     915     492     262    2269

  Fragments arranged by size: 

                2269     915     492     456     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4394
 
SphI G_CATG'C

Cuts at:    203    1447    1947     203 
   Size:       1244     500    2650

  Fragments arranged by size: 

                2650    1244     500
 
StuI AGG'CCT

Cuts at:    212    1109    1246     212 
   Size:        897     137    3360

  Fragments arranged by size: 

                3360     897     137
 
StyI C'CwwG_G

Cuts at:    107     134     180     191    1528    1882     107 
   Size:         27      46      11    1337     354    2619

  Fragments arranged by size: 

                2619    1337     354      46      27      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    361     757    1297    1977    2110    2569    2778     361 
   Size:        396     540     680     133     459     209    1977

  Fragments arranged by size: 

                1977     680     540     459     396     209     133
 
TatI w'GTAC_w

Cuts at:    215    3909     215 
   Size:       3694     700
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1213    1245    1342    1374    3911    3943    1213 
   Size:         32      97      32    2537      32    1664

  Fragments arranged by size: 

                2537    1664      97      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2191    2191 
   Size:       4394
 
XbaI T'CTAG_A

Cuts at:   1425    1425 
   Size:       4394
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1018    1383    1889    1018 
   Size:        365     506    3523

  Fragments arranged by size: 

                3523     506     365
 
XmaI C'CCGG_G

Cuts at:   1414    1414 
   Size:       4394
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PciI    PflMI     PmeI     PmlI     PpiI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BmtI   Bpu10I
    BsiWI     BsmI   BssHII  BstZ17I     ClaI    CspCI    EcoRV     FseI
    FspAI     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PfoI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     SwaI     XhoI     XmnI     ZraI
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