Restriction map for pF1KB6675
 (Linear) (Six-base) MAPSORT of: KIBB6675.seq  Check: 3903  from: 1  to: 1281



KIBB6675.orf

 With 182 enzymes: SgfI * 

                        March 22, 2013 15:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     676    1281 
   Size:        676     605
 
AleI CACnn'nnGTG

Cuts at:      0     119    1281 
   Size:        119    1162
 
AlwNI CAG_nnn'CTG

Cuts at:      0     939    1281 
   Size:        939     342
 
ApaLI G'TGCA_C

Cuts at:      0     255     537    1281 
   Size:        255     282     744

  Fragments arranged by size: 

                 744     282     255
 
AvaI C'yCGr_G

Cuts at:      0     393     731     972    1108    1281 
   Size:        393     338     241     136     173

  Fragments arranged by size: 

                 393     338     241     173     136
 
BanI G'GyrC_C

Cuts at:      0      10      79     288     648     736    1079    1281 
   Size:         10      69     209     360      88     343     202

  Fragments arranged by size: 

                 360     343     209     202      88      69      10
 
BanII G_rGCy'C

Cuts at:      0     197     275     394     613    1281 
   Size:        197      78     119     219     668

  Fragments arranged by size: 

                 668     219     197     119      78
 
BbeI G_GCGC'C

Cuts at:      0     292    1281 
   Size:        292     989
 
BglI GCCn_nnn'nGGC

Cuts at:      0      88     101     452    1204    1281 
   Size:         88      13     351     752      77

  Fragments arranged by size: 

                 752     351      88      77      13
 
Bme1580I G_kGCm'C

Cuts at:      0     259     448     541    1281 
   Size:        259     189      93     740

  Fragments arranged by size: 

                 740     259     189      93
 
BmgBI CAC'GTC

Cuts at:      0     225    1281 
   Size:        225    1056
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     365    1281 
   Size:        365     916
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     408     916    1252    1281 
   Size:        408     508     336      29

  Fragments arranged by size: 

                 508     408     336      29
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     638    1281 
   Size:        259     379     643

  Fragments arranged by size: 

                 643     379     259
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     970    1281 
   Size:        970     311
 
BsaAI yAC'GTr

Cuts at:      0     458     480    1281 
   Size:        458      22     801

  Fragments arranged by size: 

                 801     458      22
 
BsaHI Gr'CG_yC

Cuts at:      0     289     311     554     877    1196    1281 
   Size:        289      22     243     323     319      85

  Fragments arranged by size: 

                 323     319     289     243      85      22
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1105    1135    1234    1264    1281 
   Size:       1105      30      99      30      17

  Fragments arranged by size: 

                1105      99      30      30      17
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     454     580    1048    1281 
   Size:        454     126     468     233

  Fragments arranged by size: 

                 468     454     233     126
 
BseYI C'CCAG_C

Cuts at:      0     161     545     628     716    1020    1095    1166    1281 
   Size:        161     384      83      88     304      75      71     115

  Fragments arranged by size: 

                 384     304     161     115      88      83      75      71
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     483    1272    1281 
   Size:        483     789       9

  Fragments arranged by size: 

                 789     483       9
 
BsiEI CG_ry'CG

Cuts at:      0      21     239    1281 
   Size:         21     218    1042

  Fragments arranged by size: 

                1042     218      21
 
BsiHKAI G_wGCw'C

Cuts at:      0     259     541    1115    1281 
   Size:        259     282     574     166

  Fragments arranged by size: 

                 574     282     259     166
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     343    1281 
   Size:        343     938
 
Bsp1286I G_dGCh'C

Cuts at:      0     197     259     275     394     448     541     613    1115 
   Size:        197      62      16     119      54      93      72     502

Cuts at:   1115    1281 
   Size:        166

  Fragments arranged by size: 

                 502     197     166     119      93      72      62      54
                  16
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     676    1281 
   Size:        676     605
 
BsrFI r'CCGG_y

Cuts at:      0      16     860    1281 
   Size:         16     844     421

  Fragments arranged by size: 

                 844     421      16
 
BsrGI T'GTAC_A

Cuts at:      0     107    1281 
   Size:        107    1174
 
BssSI C'ACGA_G

Cuts at:      0     400    1237    1281 
   Size:        400     837      44

  Fragments arranged by size: 

                 837     400      44
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     137     254     263     506    1281 
   Size:        137     117       9     243     775

  Fragments arranged by size: 

                 775     243     137     117       9
 
BstEII G'GTnAC_C

Cuts at:      0     693     726    1281 
   Size:        693      33     555

  Fragments arranged by size: 

                 693     555      33
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     169     480     681     705    1281 
   Size:        169     311     201      24     576

  Fragments arranged by size: 

                 576     311     201     169      24
 
BstYI r'GATC_y

Cuts at:      0     569    1281 
   Size:        569     712
 
Bsu36I CC'TnA_GG

Cuts at:      0     644    1281 
   Size:        644     637
 
BtgI C'CryG_G

Cuts at:      0      26     722     923    1281 
   Size:         26     696     201     358

  Fragments arranged by size: 

                 696     358     201      26
 
BtsI GCAGTG_nn'

Cuts at:      0    1260    1262    1281 
   Size:       1260       2      19

  Fragments arranged by size: 

                1260      19       2
 
DraIII CAC_nnn'GTG

Cuts at:      0     263     434     506    1281 
   Size:        263     171      72     775

  Fragments arranged by size: 

                 775     263     171      72
 
EaeI y'GGCC_r

Cuts at:      0      18     576     862    1205    1281 
   Size:         18     558     286     343      76

  Fragments arranged by size: 

                 558     343     286      76      18
 
EagI C'GGCC_G

Cuts at:      0      18    1281 
   Size:         18    1263
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     638    1281 
   Size:        259     379     643

  Fragments arranged by size: 

                 643     379     259
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     148     816    1281 
   Size:        148     668     465

  Fragments arranged by size: 

                 668     465     148
 
EcoO109I rG'GnC_Cy

Cuts at:      0     981    1281 
   Size:        981     300
 
HaeII r_GCGC'y

Cuts at:      0     292     363     525    1187    1281 
   Size:        292      71     162     662      94

  Fragments arranged by size: 

                 662     292     162      94      71
 
HindIII A'AGCT_T

Cuts at:      0    1045    1281 
   Size:       1045     236
 
KasI G'GCGC_C

Cuts at:      0     288    1281 
   Size:        288     993
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     488     562    1281 
   Size:        245     243      74     719

  Fragments arranged by size: 

                 719     245     243      74
 
MslI CAynn'nnrTG

Cuts at:      0     119     703     922    1281 
   Size:        119     584     219     359

  Fragments arranged by size: 

                 584     359     219     119
 
MspA1I CmG'CkG

Cuts at:      0     119     323     830    1281 
   Size:        119     204     507     451

  Fragments arranged by size: 

                 507     451     204     119
 
NaeI GCC'GGC

Cuts at:      0     862    1281 
   Size:        862     419
 
NarI GG'CG_CC

Cuts at:      0     289    1281 
   Size:        289     992
 
NcoI C'CATG_G

Cuts at:      0      26    1281 
   Size:         26    1255
 
NgoMIV G'CCGG_C

Cuts at:      0     860    1281 
   Size:        860     421
 
NspI r_CATG'y

Cuts at:      0      95     285    1281 
   Size:         95     190     996

  Fragments arranged by size: 

                 996     190      95
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     723    1281 
   Size:        723     558
 
PmlI CAC'GTG

Cuts at:      0     480    1281 
   Size:        480     801
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     650     682    1281 
   Size:        650      32     599

  Fragments arranged by size: 

                 650     599      32
 
PvuII CAG'CTG

Cuts at:      0     830    1281 
   Size:        830     451
 
SfcI C'TryA_G

Cuts at:      0    1005    1281 
   Size:       1005     276
 
SfoI GGC'GCC

Cuts at:      0     290    1281 
   Size:        290     991
 
SmlI C'TyrA_G

Cuts at:      0     985    1281 
   Size:        985     296
 
SphI G_CATG'C

Cuts at:      0      95    1281 
   Size:         95    1186
 
StuI AGG'CCT

Cuts at:      0     104    1001    1138    1281 
   Size:        104     897     137     143

  Fragments arranged by size: 

                 897     143     137     104
 
StyI C'CwwG_G

Cuts at:      0      26      72      83    1281 
   Size:         26      46      11    1198

  Fragments arranged by size: 

                1198      46      26      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     253     649    1189    1281 
   Size:        253     396     540      92

  Fragments arranged by size: 

                 540     396     253      92
 
TatI w'GTAC_w

Cuts at:      0     107    1281 
   Size:        107    1174
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1105    1137    1234    1266    1281 
   Size:       1105      32      97      32      15

  Fragments arranged by size: 

                1105      97      32      32      15
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     910    1281 
   Size:        910     371
 

 Enzymes that do cut:

     AarI     AleI    AlwNI    ApaLI     AvaI     BanI    BanII     BbeI
     BglI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI    BsaAI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspMI    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI     BtsI   DraIII     EaeI     EagI  Eco57MI
    EcoNI EcoO109I    HaeII  HindIII     KasI     MmeI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI    PflMI     PmlI     PpiI
    PvuII     SfcI     SfoI     SmlI     SphI     StuI     StyI    TaqII
     TatI     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI     BmtI   Bpu10I     BsaI    BsaBI
    BsaWI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI   BssHII
    BstBI  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI     EarI
     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII     HpaI     KpnI     MfeI     MluI     MscI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
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