Restriction map for pF1KE2551
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BbeI        BbrPI       BbsI        BbvCI       BciVI       
BfrI        BglI        BlnI        BpuAI       BseAI       
BsiEI       BsiWI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstZ17I     Bsu36I      
ClaI        CpoI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoRV       EcoT14I     FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MluI        MroI        NaeI        
NarI        NcoI        NgoMIV      NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AflIII       1365  ACACGT               A^C[GA][CT]GT
AgeI          719  ACCGGT               A^CCGGT
ApaI         1492  GGGCCC               GGGCC^C
AvaI         1596  CCCGGG               C^[CT]CG[GA]G
BamHI        1426  GGATCC               G^GATCC
BanI          895  GGTGCC               G^G[CT][GA]CC
BcgI          430  CGAAATGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         995  AGATCT               A^GATCT
BlpI          214  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      214  GCTGAGC              GC^T[ACGT]AGC
BsaBI        1287  GATGTTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          238  GAGACC               GGTCTC(1/5)
BsaWI         719  ACCGGT               [AT]^CCGG[AT]
BsgI          624  CTGCAC               GTGCAG(16/14)
BsmBI           4  GAGACG               CGTCTC(1/5)
BsoBI        1596  CCCGGG               C^[CT]CG[GA]G
BsrFI         719  ACCGGT               [GA]^CCGG[CT]
CelII         214  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        719  ACCGGT               [GA]^CCGG[CT]
DraI         1810  TTTAAA               TTT^AAA
EcoRI          18  GAATTC               G^AATTC
EcoT22I       438  ATGCAT               ATGCA^T
MamI         1287  GATGTTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          945  CAATTG               C^AATTG
MunI          945  CAATTG               C^AATTG
NdeI          321  CATATG               CA^TATG
NheI         1042  GCTAGC               G^CTAGC
NsiI          438  ATGCAT               ATGCA^T
NspI         1247  ACATGC               [GA]CATG^[CT]
PinAI         719  ACCGGT               A^CCGGT
PleI          115  GAGTC                GAGTC(4/5)
PshAI        1198  GACGCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          406  GAGCTC               GAGCT^C
SmaI         1596  CCCGGG               CCC^GGG
TaqI         1520  TCGA                 T^CGA
TfiI          386  GAATC                G^A[AT]TC
TthHB8I      1520  TCGA                 T^CGA
XcmI          200  CCACCACCCTAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1596  CCCGGG               C^CCGGG
XmaI         1596  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccII         698  CGCG                 CG^CG
             1512  CGCG                
AcyI            9  GACGCC               G[GA]^CG[CT]C
              817  GGCGTC              
AfaI          138  GTAC                 GT^AC
              423  GTAC                
BclI         1337  TGATCA               T^GATCA
             1790  TGATCA              
BfaI          208  CTAG                 C^TAG
             1043  CTAG                
BsaAI        1224  CACGTA               [CT]AC^GT[GA]
             1459  TACGTG              
BsaHI           9  GACGCC               G[GA]^CG[CT]C
              817  GGCGTC              
BsmI         1881  GAATGC               GAATGC(1/-1)
              346  GCATTC              
BspMI        1414  ACCTGC               ACCTGC(4/8)
             1201  GCAGGT              
BstEII       1086  GGTGACC              G^GT[ACGT]ACC
             1434  GGTCACC             
BstPI        1086  GGTGACC              G^GT[ACGT]ACC
             1434  GGTCACC             
BstUI         698  CGCG                 CG^CG
             1512  CGCG                
DsaI         1403  CCGTGG               C^C[GA][CT]GG
             1487  CCACGG              
EarI          928  CTCTTC               CTCTTC(1/4)
             1216  CTCTTC              
EcoO65I      1086  GGTGACC              G^GT[ACGT]ACC
             1434  GGTCACC             
FbaI         1337  TGATCA               T^GATCA
             1790  TGATCA              
Hin1I           9  GACGCC               G[GA]^CG[CT]C
              817  GGCGTC              
HinfI         115  GAGTC                G^A[ACGT]TC
              386  GAATC               
Ksp632I       928  CTCTTC               CTCTTC(1/4)
             1216  CTCTTC              
MaeI          208  CTAG                 C^TAG
             1043  CTAG                
MvnI          698  CGCG                 CG^CG
             1512  CGCG                
PflMI         151  CCAAGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              735  CCAGGACCTGG         
PstI          709  CTGCAG               CTGCA^G
             1563  CTGCAG              
RsaI          138  GTAC                 GT^AC
              423  GTAC                
Van91I        151  CCAAGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              735  CCAGGACCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        AlwNI       
ApoI        AspHI       AvaII       BalI        BanII       
BbvI        BcnI        BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrBI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PpuMI       
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XhoII       
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