(Circular) (Six-base) MAPSORT of: pF1KE2438.seq Check: 7606 from: 1 to: 8936 pF1KE2438 8936 bp With 182 enzymes: SgfI * February 8, 2013 18:16 .. AccI GT'mk_AC Cuts at: 1409 5974 1409 Size: 4565 4371 Acc65I G'GTAC_C Cuts at: 5952 5952 Size: 8936 AclI AA'CG_TT Cuts at: 432 8776 432 Size: 8344 592 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2018 2339 2825 3581 4639 4729 4903 5146 5452 Size: 321 486 756 1058 90 174 243 306 Cuts at: 5452 5560 5722 6761 7193 7940 8208 2018 Size: 108 162 1039 432 747 268 2746 Fragments arranged by size: 2746 1058 1039 756 747 486 432 321 306 268 243 174 162 108 90 AfeI AGC'GCT Cuts at: 3532 3532 Size: 8936 AflII C'TTAA_G Cuts at: 946 946 Size: 8936 AflIII A'CryG_T Cuts at: 1592 4332 6288 7392 8369 1592 Size: 2740 1956 1104 977 2159 Fragments arranged by size: 2740 2159 1956 1104 977 AgeI A'CCGG_T Cuts at: 7324 7324 Size: 8936 AleI CACnn'nnGTG Cuts at: 4142 4142 Size: 8936 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 422 3451 422 Size: 3029 5907 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1717 1749 3619 3651 5225 5257 1717 Size: 32 1870 32 1574 32 5396 Fragments arranged by size: 5396 1870 1574 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 712 1284 1455 1785 2068 2659 3144 3774 3930 Size: 572 171 330 283 591 485 630 156 Cuts at: 3930 4314 4386 5271 5568 6241 7808 712 Size: 384 72 885 297 673 1567 1840 Fragments arranged by size: 1840 1567 885 673 630 591 572 485 384 330 297 283 171 156 72 ApaLI G'TGCA_C Cuts at: 7706 7706 Size: 8936 ApoI r'AATT_y Cuts at: 853 1204 2085 2395 2689 5809 5940 8289 8545 Size: 351 881 310 294 3120 131 2349 256 Cuts at: 8545 853 Size: 1244 Fragments arranged by size: 3120 2349 1244 881 351 310 294 256 131 AseI AT'TA_AT Cuts at: 20 20 Size: 8936 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 8936 AvaI C'yCGr_G Cuts at: 517 3509 3545 5956 517 Size: 2992 36 2411 3497 Fragments arranged by size: 3497 2992 2411 36 BamHI G'GATC_C Cuts at: 5961 5961 Size: 8936 BanI G'GyrC_C Cuts at: 2170 3959 5952 6366 6614 6649 2170 Size: 1789 1993 414 248 35 4457 Fragments arranged by size: 4457 1993 1789 414 248 35 BanII G_rGCy'C Cuts at: 236 1685 1989 2050 2441 5243 5950 6980 236 Size: 1449 304 61 391 2802 707 1030 2192 Fragments arranged by size: 2802 2192 1449 1030 707 391 304 61 BbeI G_GCGC'C Cuts at: 3963 6370 6618 3963 Size: 2407 248 6281 Fragments arranged by size: 6281 2407 248 BbsI GAAGACnn'nnnn_ Cuts at: 301 330 667 2422 4121 301 Size: 29 337 1755 1699 5116 Fragments arranged by size: 5116 1755 1699 337 29 BbvCI CC'TCA_GC Cuts at: 2203 2903 4325 5036 5423 5627 5771 5780 2203 Size: 700 1422 711 387 204 144 9 5359 Fragments arranged by size: 5359 1422 711 700 387 204 144 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 5208 5242 5208 Size: 34 8902 BciVI GTATCCnnnnn_n' Cuts at: 2575 4702 5073 6834 7595 8936 2575 Size: 2127 371 1761 761 1341 2575 Fragments arranged by size: 2575 2127 1761 1341 761 371 BclI T'GATC_A Cuts at: 330 635 1089 3932 5294 6456 330 Size: 305 454 2843 1362 1162 2810 Fragments arranged by size: 2843 2810 1362 1162 454 305 BfrBI ATG'CAT Cuts at: 1188 1188 Size: 8936 BglI GCCn_nnn'nGGC Cuts at: 3511 3589 3691 5395 5419 3511 Size: 78 102 1704 24 7028 Fragments arranged by size: 7028 1704 102 78 24 BglII A'GATC_T Cuts at: 1296 6451 8488 1296 Size: 5155 2037 1744 Fragments arranged by size: 5155 2037 1744 BlpI GC'TnA_GC Cuts at: 1970 2927 3095 4520 5525 6048 1970 Size: 957 168 1425 1005 523 4858 Fragments arranged by size: 4858 1425 1005 957 523 168 Bme1580I G_kGCm'C Cuts at: 2405 6561 6654 7710 2405 Size: 4156 93 1056 3631 Fragments arranged by size: 4156 3631 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 2382 6148 6229 6564 8708 2382 Size: 3766 81 335 2144 2610 Fragments arranged by size: 3766 2610 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3522 7303 3522 Size: 3781 5155 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 662 817 1261 1555 1624 1918 2974 3106 3499 Size: 155 444 294 69 294 1056 132 393 Cuts at: 3499 3574 3679 3796 4303 4723 5335 5344 5482 Size: 75 105 117 507 420 612 9 138 Cuts at: 5482 5590 5806 5854 662 Size: 108 216 48 3744 Fragments arranged by size: 3744 1056 612 507 444 420 393 294 294 216 155 138 132 117 108 105 75 69 48 9 Bpu10I CC'TnA_GC Cuts at: 2203 2903 4325 5036 5423 5627 5771 5780 2203 Size: 700 1422 711 387 204 144 9 5359 Fragments arranged by size: 5359 1422 711 700 387 204 144 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2959 3016 3310 4546 4834 4951 5059 6112 6991 Size: 57 294 1236 288 117 108 1053 879 Cuts at: 6991 7483 7781 2959 Size: 492 298 4114 Fragments arranged by size: 4114 1236 1053 879 492 298 294 288 117 108 57 BsaI GGTCTCn'nnnn_ Cuts at: 472 1161 1918 3843 4979 8640 472 Size: 689 757 1925 1136 3661 768 Fragments arranged by size: 3661 1925 1136 768 757 689 BsaAI yAC'GTr Cuts at: 6289 6289 Size: 8936 BsaBI GATnn'nnATC Cuts at: 6 6450 8487 6 Size: 6444 2037 455 Fragments arranged by size: 6444 2037 455 BsaHI Gr'CG_yC Cuts at: 3960 4259 6367 6615 7317 8813 3960 Size: 299 2108 248 702 1496 4083 Fragments arranged by size: 4083 2108 1496 702 299 248 BsaWI w'CCGG_w Cuts at: 5108 6125 6646 7324 7598 7745 8156 5108 Size: 1017 521 678 274 147 411 5888 Fragments arranged by size: 5888 1017 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 269 299 559 589 1528 1558 1717 1747 2148 Size: 30 260 30 939 30 159 30 401 Cuts at: 2148 2178 3619 3649 5225 5255 269 Size: 30 1441 30 1576 30 3950 Fragments arranged by size: 3950 1576 1441 939 401 260 159 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 320 828 1240 1627 1738 2461 3178 3487 3496 Size: 508 412 387 111 723 717 309 9 Cuts at: 3496 3571 3676 3931 4102 4183 4186 4291 4300 Size: 75 105 255 171 81 3 105 9 Cuts at: 4300 5197 5229 5440 5788 5851 320 Size: 897 32 211 348 63 3405 Fragments arranged by size: 3405 897 723 717 508 412 387 348 309 255 211 171 111 105 105 81 75 63 32 9 9 3 BseYI C'CCAG_C Cuts at: 2471 4307 6361 7119 7696 8192 2471 Size: 1836 2054 758 577 496 3215 Fragments arranged by size: 3215 2054 1836 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 674 2520 2672 5257 674 Size: 1846 152 2585 4353 Fragments arranged by size: 4353 2585 1846 152 BsiEI CG_ry'CG Cuts at: 104 6478 6524 7732 104 Size: 6374 46 1208 1308 Fragments arranged by size: 6374 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1395 1685 4206 4924 5243 5950 6728 6918 7710 Size: 290 2521 718 319 707 778 190 792 Cuts at: 7710 1395 Size: 2621 Fragments arranged by size: 2621 2521 792 778 718 707 319 290 190 BsmI GAATG_Cn' Cuts at: 3428 4587 3428 Size: 1159 7777 BsmBI CGTCTCn'nnnn_ Cuts at: 4246 4246 Size: 8936 Bsp1286I G_dGCh'C Cuts at: 236 1395 1685 1989 2050 2405 2441 4206 4924 Size: 1159 290 304 61 355 36 1765 718 Cuts at: 4924 5243 5950 6561 6654 6728 6918 6980 7710 Size: 319 707 611 93 74 190 62 730 Cuts at: 7710 236 Size: 1462 Fragments arranged by size: 1765 1462 1159 730 718 707 611 355 319 304 290 190 93 74 62 61 36 BspEI T'CCGG_A Cuts at: 5108 8156 5108 Size: 3048 5888 BspHI T'CATG_A Cuts at: 2588 8112 2588 Size: 5524 3412 BspMI ACCTGCnnnn'nnnn_ Cuts at: 3064 3161 3761 4157 5171 5986 6200 6502 6883 Size: 97 600 396 1014 815 214 302 381 Cuts at: 6883 3064 Size: 5117 Fragments arranged by size: 5117 1014 815 600 396 381 302 214 97 BsrBI CCG'CTC Cuts at: 50 3501 5466 7228 7282 8766 8931 50 Size: 3451 1965 1762 54 1484 165 55 Fragments arranged by size: 3451 1965 1762 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1344 1499 1961 3089 5379 5858 6848 1344 Size: 155 462 1128 2290 479 990 3432 Fragments arranged by size: 3432 2290 1128 990 479 462 155 BsrFI r'CCGG_y Cuts at: 3583 6934 7115 7324 3583 Size: 3351 181 209 5195 Fragments arranged by size: 5195 3351 209 181 BsrGI T'GTAC_A Cuts at: 416 416 Size: 8936 BssSI C'ACGA_G Cuts at: 7207 7565 7207 Size: 358 8578 BstAPI GCAn_nnn'nTGC Cuts at: 703 1390 4122 4386 6313 703 Size: 687 2732 264 1927 3326 Fragments arranged by size: 3326 2732 1927 687 264 BstBI TT'CG_AA Cuts at: 7297 7297 Size: 8936 BstXI CCAn_nnnn'nTGG Cuts at: 5028 5028 Size: 8936 BstYI r'GATC_y Cuts at: 1 1296 2581 4478 4796 5087 5499 5558 5961 Size: 1295 1285 1897 318 291 412 59 403 Cuts at: 5961 6428 6451 6786 7032 8044 8130 8142 8488 Size: 467 23 335 246 1012 86 12 346 Cuts at: 8488 1 Size: 449 Fragments arranged by size: 1897 1295 1285 1012 467 449 412 403 346 335 318 291 246 86 59 23 12 Bsu36I CC'TnA_GG Cuts at: 1739 2110 3700 5044 1739 Size: 371 1590 1344 5631 Fragments arranged by size: 5631 1590 1344 371 BtgI C'CryG_G Cuts at: 1928 2174 1928 Size: 246 8690 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 6886 7067 7230 6886 Size: 181 163 8592 Fragments arranged by size: 8592 181 163 BtsI GCAGTG_nn' Cuts at: 6179 6179 Size: 8936 ClaI AT'CG_AT Cuts at: 1085 1085 Size: 8936 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 2013 2048 2013 Size: 35 8901 DraI TTT'AAA Cuts at: 5935 5935 Size: 8936 DraIII CAC_nnn'GTG Cuts at: 2133 2133 Size: 8936 DrdI GACnn_nn'nnGTC Cuts at: 6642 7500 6642 Size: 858 8078 EaeI y'GGCC_r Cuts at: 380 1076 1106 1397 2672 3008 3692 3725 6521 Size: 696 30 291 1275 336 684 33 2796 Cuts at: 6521 6695 7086 7113 8458 380 Size: 174 391 27 1345 858 Fragments arranged by size: 2796 1345 1275 858 696 684 391 336 291 174 33 30 27 EagI C'GGCC_G Cuts at: 6521 6521 Size: 8936 EarI CTCTTCn'nnn_ Cuts at: 475 1715 1981 2055 2626 2641 2909 2938 3059 Size: 1240 266 74 571 15 268 29 121 Cuts at: 3059 3781 3908 4079 4781 4879 5887 5921 6959 Size: 722 127 171 702 98 1008 34 1038 Cuts at: 6959 7169 475 Size: 210 2242 Fragments arranged by size: 2242 1240 1038 1008 722 702 571 268 266 210 171 127 121 98 74 34 29 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 244 7144 7454 7600 8746 244 Size: 6900 310 146 1146 434 Fragments arranged by size: 6900 1146 434 310 146 EcoICRI GAG'CTC Cuts at: 1683 5241 5948 1683 Size: 3558 707 4671 Fragments arranged by size: 4671 3558 707 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 662 817 1261 1555 1624 1918 2018 2339 2825 Size: 155 444 294 69 294 100 321 486 Cuts at: 2825 2974 3106 3499 3574 3581 3679 3796 4303 Size: 149 132 393 75 7 98 117 507 Cuts at: 4303 4639 4723 4729 4903 5146 5335 5344 5452 Size: 336 84 6 174 243 189 9 108 Cuts at: 5452 5482 5560 5590 5722 5806 5854 6761 7193 Size: 30 78 30 132 84 48 907 432 Cuts at: 7193 7940 8208 662 Size: 747 268 1390 Fragments arranged by size: 1390 907 747 507 486 444 432 393 336 321 294 294 268 243 189 174 155 149 132 132 117 108 100 98 84 84 78 75 69 48 30 30 9 7 6 EcoNI CCTnn'n_nnAGG Cuts at: 796 1720 3661 5167 796 Size: 924 1941 1506 4565 Fragments arranged by size: 4565 1941 1506 924 EcoO109I rG'GnC_Cy Cuts at: 197 558 1911 3233 3654 4404 5040 5121 6075 Size: 361 1353 1322 421 750 636 81 954 Cuts at: 6075 197 Size: 3058 Fragments arranged by size: 3058 1353 1322 954 750 636 421 361 81 EcoRI G'AATT_C Cuts at: 5940 5940 Size: 8936 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2446 2728 6955 2446 Size: 282 4227 4427 Fragments arranged by size: 4427 4227 282 FspI TGC'GCA Cuts at: 3649 3950 4804 5647 6717 3649 Size: 301 854 843 1070 5868 Fragments arranged by size: 5868 1070 854 843 301 HaeII r_GCGC'y Cuts at: 552 3534 3963 5456 6370 6618 7640 8619 552 Size: 2982 429 1493 914 248 1022 979 869 Fragments arranged by size: 2982 1493 1022 979 914 869 429 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 378 410 610 642 823 855 948 980 2106 Size: 32 200 32 181 32 93 32 1126 Cuts at: 2106 2138 2148 2180 2622 2654 3060 3092 4165 Size: 32 10 32 442 32 406 32 1073 Cuts at: 4165 4197 5773 5805 6778 6808 6810 6840 378 Size: 32 1576 32 973 30 2 30 2474 Fragments arranged by size: 2474 1576 1126 1073 973 442 406 200 181 93 32 32 32 32 32 32 32 32 32 32 30 30 10 2 HincII GTy'rAC Cuts at: 486 3177 5975 486 Size: 2691 2798 3447 Fragments arranged by size: 3447 2798 2691 KasI G'GCGC_C Cuts at: 3959 6366 6614 3959 Size: 2407 248 6281 Fragments arranged by size: 6281 2407 248 KpnI G_GTAC'C Cuts at: 5956 5956 Size: 8936 MfeI C'AATT_G Cuts at: 701 3433 701 Size: 2732 6204 MluI A'CGCG_T Cuts at: 8369 8369 Size: 8936 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1403 1914 5816 7607 7791 1403 Size: 511 3902 1791 184 2548 Fragments arranged by size: 3902 2548 1791 511 184 MscI TGG'CCA Cuts at: 382 1078 1108 1399 2674 3010 6697 382 Size: 696 30 291 1275 336 3687 2621 Fragments arranged by size: 3687 2621 1275 696 336 291 30 MslI CAynn'nnrTG Cuts at: 398 1597 4142 5096 6925 7052 398 Size: 1199 2545 954 1829 127 2282 Fragments arranged by size: 2545 2282 1829 1199 954 127 MspA1I CmG'CkG Cuts at: 1455 1542 2202 2475 3691 3927 4311 4324 4383 Size: 87 660 273 1216 236 384 13 59 Cuts at: 4383 4881 5010 5136 5532 6047 6361 6721 7734 Size: 498 129 126 396 515 314 360 1013 Cuts at: 7734 7979 1455 Size: 245 2412 Fragments arranged by size: 2412 1216 1013 660 515 498 396 384 360 314 273 245 236 129 126 87 59 13 NaeI GCC'GGC Cuts at: 7117 7117 Size: 8936 NarI GG'CG_CC Cuts at: 3960 6367 6615 3960 Size: 2407 248 6281 Fragments arranged by size: 6281 2407 248 NcoI C'CATG_G Cuts at: 2174 2174 Size: 8936 NdeI CA'TA_TG Cuts at: 1038 1038 Size: 8936 NgoMIV G'CCGG_C Cuts at: 7115 7115 Size: 8936 NsiI A_TGCA'T Cuts at: 1190 1190 Size: 8936 NspI r_CATG'y Cuts at: 1596 1734 3034 3715 4120 4336 4519 5989 6489 Size: 138 1300 681 405 216 183 1470 500 Cuts at: 6489 7396 1596 Size: 907 3136 Fragments arranged by size: 3136 1470 1300 907 681 500 405 216 183 138 PasI CC'CwG_GG Cuts at: 1322 1322 Size: 8936 PciI A'CATG_T Cuts at: 1592 4332 7392 1592 Size: 2740 3060 3136 Fragments arranged by size: 3136 3060 2740 PflMI CCAn_nnn'nTGG Cuts at: 1455 1785 2535 4701 5568 1455 Size: 330 750 2166 867 4823 Fragments arranged by size: 4823 2166 867 750 330 PfoI T'CCnGG_A Cuts at: 1715 3122 5243 5360 1715 Size: 1407 2121 117 5291 Fragments arranged by size: 5291 2121 1407 117 PmeI GTTT'AAAC Cuts at: 5935 5935 Size: 8936 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1405 1437 1627 1659 1717 1749 3619 3651 5225 Size: 32 190 32 58 32 1870 32 1574 Cuts at: 5225 5257 8075 8107 1405 Size: 32 2818 32 2234 Fragments arranged by size: 2818 2234 1870 1574 190 58 32 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 1911 3233 3654 4404 5121 1911 Size: 1322 421 750 717 5726 Fragments arranged by size: 5726 1322 750 717 421 PsiI TTA'TAA Cuts at: 5759 5759 Size: 8936 PstI C_TGCA'G Cuts at: 1296 3158 3758 3968 4127 4199 4319 4391 5171 Size: 1862 600 210 159 72 120 72 780 Cuts at: 5171 5507 5983 6668 1296 Size: 336 476 685 3564 Fragments arranged by size: 3564 1862 780 685 600 476 336 210 159 120 72 72 PvuI CG_AT'CG Cuts at: 104 104 Size: 8936 PvuII CAG'CTG Cuts at: 1455 1542 2202 2475 4324 4881 5010 5136 5532 Size: 87 660 273 1849 557 129 126 396 Cuts at: 5532 6361 6721 1455 Size: 829 360 3670 Fragments arranged by size: 3670 1849 829 660 557 396 360 273 129 126 87 RsrII CG'GwC_CG Cuts at: 7131 7131 Size: 8936 SacI G_AGCT'C Cuts at: 1685 5243 5950 1685 Size: 3558 707 4671 Fragments arranged by size: 4671 3558 707 SalI G'TCGA_C Cuts at: 5973 5973 Size: 8936 SapI GCTCTTCn'nnn_ Cuts at: 1981 2055 5887 6959 7169 1981 Size: 74 3832 1072 210 3748 Fragments arranged by size: 3832 3748 1072 210 74 SbfI CC_TGCA'GG Cuts at: 3158 5983 3158 Size: 2825 6111 ScaI AGT'ACT Cuts at: 691 1480 8453 691 Size: 789 6973 1174 Fragments arranged by size: 6973 1174 789 SfcI C'TryA_G Cuts at: 33 1292 1364 2238 3154 3673 3754 3964 4123 Size: 1259 72 874 916 519 81 210 159 Cuts at: 4123 4195 4315 4387 5052 5167 5503 5979 6664 Size: 72 120 72 665 115 336 476 685 Cuts at: 6664 7657 7848 33 Size: 993 191 1121 Fragments arranged by size: 1259 1121 993 916 874 685 665 519 476 336 210 191 159 120 115 81 72 72 72 SfiI GGCCn_nnn'nGGCC Cuts at: 3511 5395 3511 Size: 1884 7052 SfoI GGC'GCC Cuts at: 3961 6368 6616 3961 Size: 2407 248 6281 Fragments arranged by size: 6281 2407 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 8936 SmaI CCC'GGG Cuts at: 3511 3547 5958 3511 Size: 36 2411 6489 Fragments arranged by size: 6489 2411 36 SmlI C'TyrA_G Cuts at: 946 2974 2995 3289 4525 4813 4966 5074 6091 Size: 2028 21 294 1236 288 153 108 1017 Cuts at: 6091 7006 7498 7760 946 Size: 915 492 262 2122 Fragments arranged by size: 2122 2028 1236 1017 915 492 294 288 262 153 108 21 SpeI A'CTAG_T Cuts at: 67 67 Size: 8936 SphI G_CATG'C Cuts at: 3715 4120 4519 5989 6489 3715 Size: 405 399 1470 500 6162 Fragments arranged by size: 6162 1470 500 405 399 SspI AAT'ATT Cuts at: 5706 5706 Size: 8936 StuI AGG'CCT Cuts at: 802 2347 4531 802 Size: 1545 2184 5207 Fragments arranged by size: 5207 2184 1545 StyI C'CwwG_G Cuts at: 1814 2174 2408 3011 3104 3236 3848 4184 4211 Size: 360 234 603 93 132 612 336 27 Cuts at: 4211 4554 5064 6070 6424 1814 Size: 343 510 1006 354 4326 Fragments arranged by size: 4326 1006 612 603 510 360 354 343 336 234 132 93 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 629 2401 5077 5293 6519 6652 7111 7320 629 Size: 1772 2676 216 1226 133 459 209 2245 Fragments arranged by size: 2676 2245 1772 1226 459 216 209 133 TatI w'GTAC_w Cuts at: 416 689 1478 1616 2570 8451 416 Size: 273 789 138 954 5881 901 Fragments arranged by size: 5881 954 901 789 273 138 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 269 301 559 591 1528 1560 1711 1743 8453 Size: 32 258 32 937 32 151 32 6710 Cuts at: 8453 8485 269 Size: 32 720 Fragments arranged by size: 6710 937 720 258 151 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2064 3172 6733 2064 Size: 1108 3561 4267 Fragments arranged by size: 4267 3561 1108 XbaI T'CTAG_A Cuts at: 928 3879 5967 928 Size: 2951 2088 3897 Fragments arranged by size: 3897 2951 2088 XcmI CCAnnnn_n'nnnnTGG Cuts at: 792 2395 4107 4299 5310 5457 6431 792 Size: 1603 1712 192 1011 147 974 3297 Fragments arranged by size: 3297 1712 1603 1011 974 192 147 XmaI C'CCGG_G Cuts at: 3509 3545 5956 3509 Size: 36 2411 6489 Fragments arranged by size: 6489 2411 36 XmnI GAAnn'nnTTC Cuts at: 1016 1420 2620 2690 1016 Size: 404 1200 70 7262 Fragments arranged by size: 7262 1200 404 70 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AhdI ApaI AscI AvrII BaeI BmgBI BmtI BsiWI BssHII BstEII BstZ17I EcoRV FseI FspAI HindIII HpaI NheI NotI NruI PacI PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SwaI XhoI ZraI