Restriction map for pF1KE2438
 (Linear) (Six-base) MAPSORT of: KIEE2438.seq  Check: 7004  from: 1  to: 5823



KIEE2438.orf

 With 182 enzymes: SgfI * 

                        February 8, 2013 18:16  ..

AccI GT'mk_AC

Cuts at:      0    1301    5823 
   Size:       1301    4522
 
AclI AA'CG_TT

Cuts at:      0     324    5823 
   Size:        324    5499
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1910    2231    2717    3473    4531    4621    4795    5038 
   Size:       1910     321     486     756    1058      90     174     243

Cuts at:   5038    5344    5452    5614    5823 
   Size:        306     108     162     209

  Fragments arranged by size: 

                1910    1058     756     486     321     306     243     209
                 174     162     108      90
 
AfeI AGC'GCT

Cuts at:      0    3424    5823 
   Size:       3424    2399
 
AflII C'TTAA_G

Cuts at:      0     838    5823 
   Size:        838    4985
 
AflIII A'CryG_T

Cuts at:      0    1484    4224    5823 
   Size:       1484    2740    1599

  Fragments arranged by size: 

                2740    1599    1484
 
AleI CACnn'nnGTG

Cuts at:      0    4034    5823 
   Size:       4034    1789
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     314    3343    5823 
   Size:        314    3029    2480

  Fragments arranged by size: 

                3029    2480     314
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1609    1641    3511    3543    5117    5149    5823 
   Size:       1609      32    1870      32    1574      32     674

  Fragments arranged by size: 

                1870    1609    1574     674      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     604    1176    1347    1677    1960    2551    3036    3666 
   Size:        604     572     171     330     283     591     485     630

Cuts at:   3666    3822    4206    4278    5163    5460    5823 
   Size:        156     384      72     885     297     363

  Fragments arranged by size: 

                 885     630     604     591     572     485     384     363
                 330     297     283     171     156      72
 
ApoI r'AATT_y

Cuts at:      0     745    1096    1977    2287    2581    5701    5823 
   Size:        745     351     881     310     294    3120     122

  Fragments arranged by size: 

                3120     881     745     351     310     294     122
 
AvaI C'yCGr_G

Cuts at:      0     409    3401    3437    5823 
   Size:        409    2992      36    2386

  Fragments arranged by size: 

                2992    2386     409      36
 
BanI G'GyrC_C

Cuts at:      0    2062    3851    5823 
   Size:       2062    1789    1972

  Fragments arranged by size: 

                2062    1972    1789
 
BanII G_rGCy'C

Cuts at:      0     128    1577    1881    1942    2333    5135    5823 
   Size:        128    1449     304      61     391    2802     688

  Fragments arranged by size: 

                2802    1449     688     391     304     128      61
 
BbeI G_GCGC'C

Cuts at:      0    3855    5823 
   Size:       3855    1968
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     193     222     559    2314    4013    5823 
   Size:        193      29     337    1755    1699    1810

  Fragments arranged by size: 

                1810    1755    1699     337     193      29
 
BbvCI CC'TCA_GC

Cuts at:      0    2095    2795    4217    4928    5315    5519    5663    5672 
   Size:       2095     700    1422     711     387     204     144       9

Cuts at:   5672    5823 
   Size:        151

  Fragments arranged by size: 

                2095    1422     711     700     387     204     151     144
                   9
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    5100    5134    5823 
   Size:       5100      34     689

  Fragments arranged by size: 

                5100     689      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2467    4594    4965    5823 
   Size:       2467    2127     371     858

  Fragments arranged by size: 

                2467    2127     858     371
 
BclI T'GATC_A

Cuts at:      0     222     527     981    3824    5186    5823 
   Size:        222     305     454    2843    1362     637

  Fragments arranged by size: 

                2843    1362     637     454     305     222
 
BfrBI ATG'CAT

Cuts at:      0    1080    5823 
   Size:       1080    4743
 
BglI GCCn_nnn'nGGC

Cuts at:      0    3403    3481    3583    5287    5311    5823 
   Size:       3403      78     102    1704      24     512

  Fragments arranged by size: 

                3403    1704     512     102      78      24
 
BglII A'GATC_T

Cuts at:      0    1188    5823 
   Size:       1188    4635
 
BlpI GC'TnA_GC

Cuts at:      0    1862    2819    2987    4412    5417    5823 
   Size:       1862     957     168    1425    1005     406

  Fragments arranged by size: 

                1862    1425    1005     957     406     168
 
Bme1580I G_kGCm'C

Cuts at:      0    2297    5823 
   Size:       2297    3526
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2274    5823 
   Size:       2274    3549
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3414    5823 
   Size:       3414    2409
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     554     709    1153    1447    1516    1810    2866    2998 
   Size:        554     155     444     294      69     294    1056     132

Cuts at:   2998    3391    3466    3571    3688    4195    4615    5227    5236 
   Size:        393      75     105     117     507     420     612       9

Cuts at:   5236    5374    5482    5698    5746    5823 
   Size:        138     108     216      48      77

  Fragments arranged by size: 

                1056     612     554     507     444     420     393     294
                 294     216     155     138     132     117     108     105
                  77      75      69      48       9
 
Bpu10I CC'TnA_GC

Cuts at:      0    2095    2795    4217    4928    5315    5519    5663    5672 
   Size:       2095     700    1422     711     387     204     144       9

Cuts at:   5672    5823 
   Size:        151

  Fragments arranged by size: 

                2095    1422     711     700     387     204     151     144
                   9
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2851    2908    3202    4438    4726    4843    4951    5823 
   Size:       2851      57     294    1236     288     117     108     872

  Fragments arranged by size: 

                2851    1236     872     294     288     117     108      57
            
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     364    1053    1810    3735    4871    5823 
   Size:        364     689     757    1925    1136     952

  Fragments arranged by size: 

                1925    1136     952     757     689     364
 
BsaHI Gr'CG_yC

Cuts at:      0    3852    4151    5823 
   Size:       3852     299    1672

  Fragments arranged by size: 

                3852    1672     299
 
BsaWI w'CCGG_w

Cuts at:      0    5000    5823 
   Size:       5000     823
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     161     191     451     481    1420    1450    1609    1639 
   Size:        161      30     260      30     939      30     159      30

Cuts at:   1639    2040    2070    3511    3541    5117    5147    5823 
   Size:        401      30    1441      30    1576      30     676

  Fragments arranged by size: 

                1576    1441     939     676     401     260     161     159
                  30      30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     212     720    1132    1519    1630    2353    3070    3379 
   Size:        212     508     412     387     111     723     717     309

Cuts at:   3379    3388    3463    3568    3823    3994    4075    4078    4183 
   Size:          9      75     105     255     171      81       3     105

Cuts at:   4183    4192    5089    5121    5332    5680    5743    5823 
   Size:          9     897      32     211     348      63      80

  Fragments arranged by size: 

                 897     723     717     508     412     387     348     309
                 255     212     211     171     111     105     105      81
                  80      75      63      32       9       9       3
 
BseYI C'CCAG_C

Cuts at:      0    2363    4199    5823 
   Size:       2363    1836    1624

  Fragments arranged by size: 

                2363    1836    1624
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     566    2412    2564    5149    5823 
   Size:        566    1846     152    2585     674

  Fragments arranged by size: 

                2585    1846     674     566     152
 
BsiHKAI G_wGCw'C

Cuts at:      0    1287    1577    4098    4816    5135    5823 
   Size:       1287     290    2521     718     319     688

  Fragments arranged by size: 

                2521    1287     718     688     319     290
 
BsmI GAATG_Cn'

Cuts at:      0    3320    4479    5823 
   Size:       3320    1159    1344

  Fragments arranged by size: 

                3320    1344    1159
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    4138    5823 
   Size:       4138    1685
 
Bsp1286I G_dGCh'C

Cuts at:      0     128    1287    1577    1881    1942    2297    2333    4098 
   Size:        128    1159     290     304      61     355      36    1765

Cuts at:   4098    4816    5135    5823 
   Size:        718     319     688

  Fragments arranged by size: 

                1765    1159     718     688     355     319     304     290
                 128      61      36
 
BspEI T'CCGG_A

Cuts at:      0    5000    5823 
   Size:       5000     823
 
BspHI T'CATG_A

Cuts at:      0    2480    5823 
   Size:       2480    3343
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2956    3053    3653    4049    5063    5823 
   Size:       2956      97     600     396    1014     760

  Fragments arranged by size: 

                2956    1014     760     600     396      97
 
BsrBI CCG'CTC

Cuts at:      0    3393    5358    5823 
   Size:       3393    1965     465

  Fragments arranged by size: 

                3393    1965     465
 
BsrDI GCAATG_nn'

Cuts at:      0    1236    1391    1853    2981    5271    5750    5823 
   Size:       1236     155     462    1128    2290     479      73

  Fragments arranged by size: 

                2290    1236    1128     479     462     155      73
 
BsrFI r'CCGG_y

Cuts at:      0    3475    5823 
   Size:       3475    2348
 
BsrGI T'GTAC_A

Cuts at:      0     308    5823 
   Size:        308    5515
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     595    1282    4014    4278    5823 
   Size:        595     687    2732     264    1545

  Fragments arranged by size: 

                2732    1545     687     595     264
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    4920    5823 
   Size:       4920     903
 
BstYI r'GATC_y

Cuts at:      0    1188    2473    4370    4688    4979    5391    5450    5823 
   Size:       1188    1285    1897     318     291     412      59     373

  Fragments arranged by size: 

                1897    1285    1188     412     373     318     291      59
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    1631    2002    3592    4936    5823 
   Size:       1631     371    1590    1344     887

  Fragments arranged by size: 

                1631    1590    1344     887     371
 
BtgI C'CryG_G

Cuts at:      0    1820    2066    5823 
   Size:       1820     246    3757

  Fragments arranged by size: 

                3757    1820     246
 
ClaI AT'CG_AT

Cuts at:      0     977    5823 
   Size:        977    4846
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1905    1940    5823 
   Size:       1905      35    3883

  Fragments arranged by size: 

                3883    1905      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2025    5823 
   Size:       2025    3798
 
EaeI y'GGCC_r

Cuts at:      0     272     968     998    1289    2564    2900    3584    3617 
   Size:        272     696      30     291    1275     336     684      33

Cuts at:   3617    5823 
   Size:       2206

  Fragments arranged by size: 

                2206    1275     696     684     336     291     272      33
                  30
 
EarI CTCTTCn'nnn_

Cuts at:      0     367    1607    1873    1947    2518    2533    2801    2830 
   Size:        367    1240     266      74     571      15     268      29

Cuts at:   2830    2951    3673    3800    3971    4673    4771    5779    5813 
   Size:        121     722     127     171     702      98    1008      34

Cuts at:   5813    5823 
   Size:         10

  Fragments arranged by size: 

                1240    1008     722     702     571     367     268     266
                 171     127     121      98      74      34      29      15
                  10
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     136    5823 
   Size:        136    5687
 
EcoICRI GAG'CTC

Cuts at:      0    1575    5133    5823 
   Size:       1575    3558     690

  Fragments arranged by size: 

                3558    1575     690
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     554     709    1153    1447    1516    1810    1910    2231 
   Size:        554     155     444     294      69     294     100     321

Cuts at:   2231    2717    2866    2998    3391    3466    3473    3571    3688 
   Size:        486     149     132     393      75       7      98     117

Cuts at:   3688    4195    4531    4615    4621    4795    5038    5227    5236 
   Size:        507     336      84       6     174     243     189       9

Cuts at:   5236    5344    5374    5452    5482    5614    5698    5746    5823 
   Size:        108      30      78      30     132      84      48      77

  Fragments arranged by size: 

                 554     507     486     444     393     336     321     294
                 294     243     189     174     155     149     132     132
                 117     108     100      98      84      84      78      77
                  75      69      48      30      30       9       7       6
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     688    1612    3553    5059    5823 
   Size:        688     924    1941    1506     764

  Fragments arranged by size: 

                1941    1506     924     764     688
 
EcoO109I rG'GnC_Cy

Cuts at:      0      89     450    1803    3125    3546    4296    4932    5013 
   Size:         89     361    1353    1322     421     750     636      81

Cuts at:   5013    5823 
   Size:        810

  Fragments arranged by size: 

                1353    1322     810     750     636     421     361      89
                  81
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2338    2620    5823 
   Size:       2338     282    3203

  Fragments arranged by size: 

                3203    2338     282
 
FspI TGC'GCA

Cuts at:      0    3541    3842    4696    5539    5823 
   Size:       3541     301     854     843     284

  Fragments arranged by size: 

                3541     854     843     301     284
 
HaeII r_GCGC'y

Cuts at:      0     444    3426    3855    5348    5823 
   Size:        444    2982     429    1493     475

  Fragments arranged by size: 

                2982    1493     475     444     429
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     270     302     502     534     715     747     840     872 
   Size:        270      32     200      32     181      32      93      32

Cuts at:    872    1998    2030    2040    2072    2514    2546    2952    2984 
   Size:       1126      32      10      32     442      32     406      32

Cuts at:   2984    4057    4089    5665    5697    5823 
   Size:       1073      32    1576      32     126

  Fragments arranged by size: 

                1576    1126    1073     442     406     270     200     181
                 126      93      32      32      32      32      32      32
                  32      32      32      32      10
 
HincII GTy'rAC

Cuts at:      0     378    3069    5823 
   Size:        378    2691    2754

  Fragments arranged by size: 

                2754    2691     378
 
KasI G'GCGC_C

Cuts at:      0    3851    5823 
   Size:       3851    1972
 
MfeI C'AATT_G

Cuts at:      0     593    3325    5823 
   Size:        593    2732    2498

  Fragments arranged by size: 

                2732    2498     593
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1295    1806    5708    5823 
   Size:       1295     511    3902     115

  Fragments arranged by size: 

                3902    1295     511     115
 
MscI TGG'CCA

Cuts at:      0     274     970    1000    1291    2566    2902    5823 
   Size:        274     696      30     291    1275     336    2921

  Fragments arranged by size: 

                2921    1275     696     336     291     274      30
 
MslI CAynn'nnrTG

Cuts at:      0     290    1489    4034    4988    5823 
   Size:        290    1199    2545     954     835

  Fragments arranged by size: 

                2545    1199     954     835     290
 
MspA1I CmG'CkG

Cuts at:      0    1347    1434    2094    2367    3583    3819    4203    4216 
   Size:       1347      87     660     273    1216     236     384      13

Cuts at:   4216    4275    4773    4902    5028    5424    5823 
   Size:         59     498     129     126     396     399

  Fragments arranged by size: 

                1347    1216     660     498     399     396     384     273
                 236     129     126      87      59      13
 
NarI GG'CG_CC

Cuts at:      0    3852    5823 
   Size:       3852    1971
 
NcoI C'CATG_G

Cuts at:      0    2066    5823 
   Size:       2066    3757
 
NdeI CA'TA_TG

Cuts at:      0     930    5823 
   Size:        930    4893
 
NsiI A_TGCA'T

Cuts at:      0    1082    5823 
   Size:       1082    4741
 
NspI r_CATG'y

Cuts at:      0    1488    1626    2926    3607    4012    4228    4411    5823 
   Size:       1488     138    1300     681     405     216     183    1412

  Fragments arranged by size: 

                1488    1412    1300     681     405     216     183     138
            
 
PasI CC'CwG_GG

Cuts at:      0    1214    5823 
   Size:       1214    4609
 
PciI A'CATG_T

Cuts at:      0    1484    4224    5823 
   Size:       1484    2740    1599

  Fragments arranged by size: 

                2740    1599    1484
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1347    1677    2427    4593    5460    5823 
   Size:       1347     330     750    2166     867     363

  Fragments arranged by size: 

                2166    1347     867     750     363     330
 
PfoI T'CCnGG_A

Cuts at:      0    1607    3014    5135    5252    5823 
   Size:       1607    1407    2121     117     571

  Fragments arranged by size: 

                2121    1607    1407     571     117
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1297    1329    1519    1551    1609    1641    3511    3543 
   Size:       1297      32     190      32      58      32    1870      32

Cuts at:   3543    5117    5149    5823 
   Size:       1574      32     674

  Fragments arranged by size: 

                1870    1574    1297     674     190      58      32      32
                  32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1803    3125    3546    4296    5013    5823 
   Size:       1803    1322     421     750     717     810

  Fragments arranged by size: 

                1803    1322     810     750     717     421
 
PsiI TTA'TAA

Cuts at:      0    5651    5823 
   Size:       5651     172
 
PstI C_TGCA'G

Cuts at:      0    1188    3050    3650    3860    4019    4091    4211    4283 
   Size:       1188    1862     600     210     159      72     120      72

Cuts at:   4283    5063    5399    5823 
   Size:        780     336     424

  Fragments arranged by size: 

                1862    1188     780     600     424     336     210     159
                 120      72      72
 
PvuII CAG'CTG

Cuts at:      0    1347    1434    2094    2367    4216    4773    4902    5028 
   Size:       1347      87     660     273    1849     557     129     126

Cuts at:   5028    5424    5823 
   Size:        396     399

  Fragments arranged by size: 

                1849    1347     660     557     399     396     273     129
                 126      87
 
SacI G_AGCT'C

Cuts at:      0    1577    5135    5823 
   Size:       1577    3558     688

  Fragments arranged by size: 

                3558    1577     688
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1873    1947    5779    5823 
   Size:       1873      74    3832      44

  Fragments arranged by size: 

                3832    1873      74      44
 
SbfI CC_TGCA'GG

Cuts at:      0    3050    5823 
   Size:       3050    2773
 
ScaI AGT'ACT

Cuts at:      0     583    1372    5823 
   Size:        583     789    4451

  Fragments arranged by size: 

                4451     789     583
 
SfcI C'TryA_G

Cuts at:      0    1184    1256    2130    3046    3565    3646    3856    4015 
   Size:       1184      72     874     916     519      81     210     159

Cuts at:   4015    4087    4207    4279    4944    5059    5395    5823 
   Size:         72     120      72     665     115     336     428

  Fragments arranged by size: 

                1184     916     874     665     519     428     336     210
                 159     120     115      81      72      72      72
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    3403    5287    5823 
   Size:       3403    1884     536

  Fragments arranged by size: 

                3403    1884     536
 
SfoI GGC'GCC

Cuts at:      0    3853    5823 
   Size:       3853    1970
 
SmaI CCC'GGG

Cuts at:      0    3403    3439    5823 
   Size:       3403      36    2384

  Fragments arranged by size: 

                3403    2384      36
 
SmlI C'TyrA_G

Cuts at:      0     838    2866    2887    3181    4417    4705    4858    4966 
   Size:        838    2028      21     294    1236     288     153     108

Cuts at:   4966    5823 
   Size:        857

  Fragments arranged by size: 

                2028    1236     857     838     294     288     153     108
                  21
 
SphI G_CATG'C

Cuts at:      0    3607    4012    4411    5823 
   Size:       3607     405     399    1412

  Fragments arranged by size: 

                3607    1412     405     399
 
SspI AAT'ATT

Cuts at:      0    5598    5823 
   Size:       5598     225
 
StuI AGG'CCT

Cuts at:      0     694    2239    4423    5823 
   Size:        694    1545    2184    1400

  Fragments arranged by size: 

                2184    1545    1400     694
 
StyI C'CwwG_G

Cuts at:      0    1706    2066    2300    2903    2996    3128    3740    4076 
   Size:       1706     360     234     603      93     132     612     336

Cuts at:   4076    4103    4446    4956    5823 
   Size:         27     343     510     867

  Fragments arranged by size: 

                1706     867     612     603     510     360     343     336
                 234     132      93      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     521    2293    4969    5185    5823 
   Size:        521    1772    2676     216     638

  Fragments arranged by size: 

                2676    1772     638     521     216
 
TatI w'GTAC_w

Cuts at:      0     308     581    1370    1508    2462    5823 
   Size:        308     273     789     138     954    3361

  Fragments arranged by size: 

                3361     954     789     308     273     138
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     161     193     451     483    1420    1452    1603    1635 
   Size:        161      32     258      32     937      32     151      32

Cuts at:   1635    5823 
   Size:       4188

  Fragments arranged by size: 

                4188     937     258     161     151      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1956    3064    5823 
   Size:       1956    1108    2759

  Fragments arranged by size: 

                2759    1956    1108
 
XbaI T'CTAG_A

Cuts at:      0     820    3771    5823 
   Size:        820    2951    2052

  Fragments arranged by size: 

                2951    2052     820
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     684    2287    3999    4191    5202    5349    5823 
   Size:        684    1603    1712     192    1011     147     474

  Fragments arranged by size: 

                1712    1603    1011     684     474     192     147
 
XmaI C'CCGG_G

Cuts at:      0    3401    3437    5823 
   Size:       3401      36    2386

  Fragments arranged by size: 

                3401    2386      36
 
XmnI GAAnn'nnTTC

Cuts at:      0     908    1312    2512    2582    5823 
   Size:        908     404    1200      70    3241

  Fragments arranged by size: 

                3241    1200     908     404      70
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AfeI    AflII   AflIII     AleI     AlfI
     AloI    AlwNI     ApoI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI     ClaI    CspCI   DraIII
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
     FspI    HaeII    Hin4I   HincII     KasI     MfeI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     NdeI     NsiI     NspI     PasI
     PciI    PflMI     PfoI     PpiI    PpuMI     PsiI     PstI    PvuII
     SacI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SmaI
     SmlI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AgeI     AhdI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BmgBI     BmtI    BsaAI
    BsaBI    BsiEI    BsiWI   BssHII    BssSI    BstBI   BstEII  BstZ17I
    BtgZI     BtsI     DraI     DrdI     EagI    EcoRI    EcoRV     FseI
    FspAI  HindIII     HpaI     KpnI     MluI     NaeI   NgoMIV     NheI
     NotI     NruI     PacI     PmeI     PmlI    PshAI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI    SexAI     SgfI
    SgrAI    SnaBI     SpeI     SrfI     SwaI     XhoI     ZraI
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