Restriction map for pF1KB4192
 (Circular) (Six-base) MAPSORT of: pF1KB4192.seq  Check: 6171  from: 1  to: 4592



pF1KB4192  4592 bp

 With 182 enzymes: SgfI * 

                        January 17, 2012 13:59  ..

AatII G_ACGT'C

Cuts at:    797     797 
   Size:       4592
 
AccI GT'mk_AC

Cuts at:    497     653    1630     497 
   Size:        156     977    3459

  Fragments arranged by size: 

                3459     977     156
 
Acc65I G'GTAC_C

Cuts at:   1608    1608 
   Size:       4592
 
AclI AA'CG_TT

Cuts at:   4432    4432 
   Size:       4592
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    395    1488    2417    2849    3596    3864     395 
   Size:       1093     929     432     747     268    1123

  Fragments arranged by size: 

                1123    1093     929     747     432     268
 
AflIII A'CryG_T

Cuts at:   1088    1944    3048    4025    1088 
   Size:        856    1104     977    1655

  Fragments arranged by size: 

                1655    1104     977     856
 
AgeI A'CCGG_T

Cuts at:   2980    2980 
   Size:       4592
 
AleI CACnn'nnGTG

Cuts at:    761     761 
   Size:       4592
 
AlwNI CAG_nnn'CTG

Cuts at:    548    1344    1897    3464     548 
   Size:        796     553    1567    1676

  Fragments arranged by size: 

                1676    1567     796     553
 
ApaLI G'TGCA_C

Cuts at:   3362    3362 
   Size:       4592
 
ApoI r'AATT_y

Cuts at:   1596    3945    4201    1596 
   Size:       2349     256    1987

  Fragments arranged by size: 

                2349    1987     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4592
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4592
 
AvaI C'yCGr_G

Cuts at:    738     870    1186    1196    1360    1531    1612     738 
   Size:        132     316      10     164     171      81    3718

  Fragments arranged by size: 

                3718     316     171     164     132      81      10
 
BamHI G'GATC_C

Cuts at:   1617    1617 
   Size:       4592
 
BanI G'GyrC_C

Cuts at:    766    1304    1348    1608    2022    2270    2305     766 
   Size:        538      44     260     414     248      35    3053

  Fragments arranged by size: 

                3053     538     414     260     248      44      35
 
BanII G_rGCy'C

Cuts at:    120    1174    1286    1516    1606    2636     120 
   Size:       1054     112     230      90    1030    2076

  Fragments arranged by size: 

                2076    1054    1030     230     112      90
 
BbeI G_GCGC'C

Cuts at:    770    1352    2026    2274     770 
   Size:        582     674     248    3088

  Fragments arranged by size: 

                3088     674     582     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    954     954 
   Size:       4592
 
BciVI GTATCCnnnnn_n'

Cuts at:    240    1588    2490    3251    4592     240 
   Size:       1348     902     761    1341     240

  Fragments arranged by size: 

                1348    1341     902     761     240
 
BclI T'GATC_A

Cuts at:   2112    2112 
   Size:       4592
 
BglI GCCn_nnn'nGGC

Cuts at:   1169    1198    1215    1169 
   Size:         29      17    4546

  Fragments arranged by size: 

                4546      29      17
 
BglII A'GATC_T

Cuts at:   2107    4144    2107 
   Size:       2037    2555
 
BlpI GC'TnA_GC

Cuts at:   1704    1704 
   Size:       4592
 
Bme1580I G_kGCm'C

Cuts at:    857    2217    2310    3366     857 
   Size:       1360      93    1056    2083

  Fragments arranged by size: 

                2083    1360    1056      93
 
BmgBI CAC'GTC

Cuts at:    518     518 
   Size:       4592
 
BmrI ACTGGGnnnn_n'

Cuts at:   1455    1804    1885    2220    4364    1455 
   Size:        349      81     335    2144    1683

  Fragments arranged by size: 

                2144    1683     349     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    525    2959     525 
   Size:       2434    2158
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1168    1500    1168 
   Size:        332    4260
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    250    1768    2647    3139    3437     250 
   Size:       1518     879     492     298    1405

  Fragments arranged by size: 

                1518    1405     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    936    4296     936 
   Size:       3360    1232
 
BsaAI yAC'GTr

Cuts at:   1945    1945 
   Size:       4592
 
BsaBI GATnn'nnATC

Cuts at:      6     337     685    2106    4143       6 
   Size:        331     348    1421    2037     455

  Fragments arranged by size: 

                2037    1421     455     348     331
 
BsaHI Gr'CG_yC

Cuts at:    482     767     794    1102    1222    1349    2023    2271    2973 
   Size:        285      27     308     120     127     674     248     702

Cuts at:   2973    4469     482 
   Size:       1496     605

  Fragments arranged by size: 

                1496     702     674     605     308     285     248     127
                 120      27
 
BsaWI w'CCGG_w

Cuts at:   1781    2302    2980    3254    3401    3812    1781 
   Size:        521     678     274     147     411    2561

  Fragments arranged by size: 

                2561     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    127     322    1267     127 
   Size:        195     945    3452

  Fragments arranged by size: 

                3452     945     195
 
BseYI C'CCAG_C

Cuts at:   1094    2017    2775    3352    3848    1094 
   Size:        923     758     577     496    1838

  Fragments arranged by size: 

                1838     923     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    2134    2180    3388     104 
   Size:       2030      46    1208    1308

  Fragments arranged by size: 

                2030    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    494     866    1085    1286    1408    1502    1606    2384    2574 
   Size:        372     219     201     122      94     104     778     190

Cuts at:   2574    3366     494 
   Size:        792    1720

  Fragments arranged by size: 

                1720     792     778     372     219     201     190     122
                 104      94
 
BsmI GAATG_Cn'

Cuts at:   1467    1467 
   Size:       4592
 
Bsp1286I G_dGCh'C

Cuts at:    120     494     857     866    1085    1174    1286    1408    1502 
   Size:        374     363       9     219      89     112     122      94

Cuts at:   1502    1516    1606    2217    2310    2384    2574    2636    3366 
   Size:         14      90     611      93      74     190      62     730

Cuts at:   3366     120 
   Size:       1346

  Fragments arranged by size: 

                1346     730     611     374     363     219     190     122
                 112      94      93      90      89      74      62      14
                   9
 
BspEI T'CCGG_A

Cuts at:   3812    3812 
   Size:       4592
 
BspHI T'CATG_A

Cuts at:   1064    3768    1064 
   Size:       2704    1888
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1013    1642    1856    2158    2539    1013 
   Size:        629     214     302     381    3066

  Fragments arranged by size: 

                3066     629     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     608     828    1014    1492    2884    2938    4422    4587 
   Size:        558     220     186     478    1392      54    1484     165

Cuts at:   4587      50 
   Size:         55

  Fragments arranged by size: 

                1484    1392     558     478     220     186     165      55
                  54
 
BsrDI GCAATG_nn'

Cuts at:    974    2504     974 
   Size:       1530    3062
 
BsrFI r'CCGG_y

Cuts at:    224     900     910    1307    1426    2590    2771    2980     224 
   Size:        676      10     397     119    1164     181     209    1836

  Fragments arranged by size: 

                1836    1164     676     397     209     181     119      10
            
 
BssSI C'ACGA_G

Cuts at:    418    1285    1407    2863    3221     418 
   Size:        867     122    1456     358    1789

  Fragments arranged by size: 

                1789    1456     867     358     122
 
BstAPI GCAn_nnn'nTGC

Cuts at:    548    1969     548 
   Size:       1421    3171
 
BstBI TT'CG_AA

Cuts at:   2953    2953 
   Size:       4592
 
BstEII G'GTnAC_C

Cuts at:    270     561     270 
   Size:        291    4301
 
BstXI CCAn_nnnn'nTGG

Cuts at:    763     763 
   Size:       4592
 
BstYI r'GATC_y

Cuts at:      1    1617    2084    2107    2442    2688    3700    3786    3798 
   Size:       1616     467      23     335     246    1012      86      12

Cuts at:   3798    4144       1 
   Size:        346     449

  Fragments arranged by size: 

                1616    1012     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    242     242 
   Size:       4592
 
BtgI C'CryG_G

Cuts at:    107     759    1126    1273     107 
   Size:        652     367     147    3426

  Fragments arranged by size: 

                3426     652     367     147
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2542    2723    2886    2542 
   Size:        181     163    4248

  Fragments arranged by size: 

                4248     181     163
 
BtsI GCAGTG_nn'

Cuts at:    560    1835     560 
   Size:       1275    3317
 
ClaI AT'CG_AT

Cuts at:    341     341 
   Size:       4592
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1041    1076    1041 
   Size:         35    4557
 
DraI TTT'AAA

Cuts at:   1591    1591 
   Size:       4592
 
DrdI GACnn_nn'nnGTC

Cuts at:   2298    3156    2298 
   Size:        858    3734
 
EaeI y'GGCC_r

Cuts at:    226     459     530     915    2177    2351    2742    2769    4114 
   Size:        233      71     385    1262     174     391      27    1345

Cuts at:   4114     226 
   Size:        704

  Fragments arranged by size: 

                1345    1262     704     391     385     233     174      71
                  27
 
EagI C'GGCC_G

Cuts at:   2177    2177 
   Size:       4592
 
EarI CTCTTCn'nnn_

Cuts at:    205     623     835    1507    2615    2825     205 
   Size:        418     212     672    1108     210    1972

  Fragments arranged by size: 

                1972    1108     672     418     212     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    739     805    2800    3110    3256    4402     739 
   Size:         66    1995     310     146    1146     929

  Fragments arranged by size: 

                1995    1146     929     310     146      66
 
EcoICRI GAG'CTC

Cuts at:   1284    1604    1284 
   Size:        320    4272
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    395    1168    1488    1500    2417    2849    3596    3864     395 
   Size:        773     320      12     917     432     747     268    1123

  Fragments arranged by size: 

                1123     917     773     747     432     320     268      12
            
 
EcoO109I rG'GnC_Cy

Cuts at:    129    1191    1316    1535    1731     129 
   Size:       1062     125     219     196    2990

  Fragments arranged by size: 

                2990    1062     219     196     125
 
EcoRI G'AATT_C

Cuts at:   1596    1596 
   Size:       4592
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    396     846    2611     396 
   Size:        450    1765    2377

  Fragments arranged by size: 

                2377    1765     450
 
FspI TGC'GCA

Cuts at:    552    1453    2373     552 
   Size:        901     920    2771

  Fragments arranged by size: 

                2771     920     901
 
FspAI rTGC'GCAy

Cuts at:   1453    1453 
   Size:       4592
 
HaeII r_GCGC'y

Cuts at:    770    1352    2026    2274    3296    4275     770 
   Size:        582     674     248    1022     979    1087

  Fragments arranged by size: 

                1087    1022     979     674     582     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1423    1455    2434    2464    2466    2496    1423 
   Size:         32     979      30       2      30    3519

  Fragments arranged by size: 

                3519     979      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1631    1631 
   Size:       4592
 
KasI G'GCGC_C

Cuts at:    766    1348    2022    2270     766 
   Size:        582     674     248    3088

  Fragments arranged by size: 

                3088     674     582     248
 
KpnI G_GTAC'C

Cuts at:   1612    1612 
   Size:       4592
 
MluI A'CGCG_T

Cuts at:   4025    4025 
   Size:       4592
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1466    3263    3447    1466 
   Size:       1797     184    2611

  Fragments arranged by size: 

                2611    1797     184
 
MscI TGG'CCA

Cuts at:    461    2353     461 
   Size:       1892    2700
 
MslI CAynn'nnrTG

Cuts at:    761     850    1063    2581    2708     761 
   Size:         89     213    1518     127    2645

  Fragments arranged by size: 

                2645    1518     213     127      89
 
MspA1I CmG'CkG

Cuts at:   1098    1128    1370    1703    2017    2377    3390    3635    1098 
   Size:         30     242     333     314     360    1013     245    2055

  Fragments arranged by size: 

                2055    1013     360     333     314     245     242      30
            
 
NaeI GCC'GGC

Cuts at:    226     902     912    2773     226 
   Size:        676      10    1861    2045

  Fragments arranged by size: 

                2045    1861     676      10
 
NarI GG'CG_CC

Cuts at:    767    1349    2023    2271     767 
   Size:        582     674     248    3088

  Fragments arranged by size: 

                3088     674     582     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4592
 
NgoMIV G'CCGG_C

Cuts at:    224     900     910    2771     224 
   Size:        676      10    1861    2045

  Fragments arranged by size: 

                2045    1861     676      10
 
NspI r_CATG'y

Cuts at:   1645    2145    3052    1645 
   Size:        500     907    3185

  Fragments arranged by size: 

                3185     907     500
 
PasI CC'CwG_GG

Cuts at:    781     781 
   Size:       4592
 
PciI A'CATG_T

Cuts at:   3048    3048 
   Size:       4592
 
PflMI CCAn_nnn'nTGG

Cuts at:   1344    1344 
   Size:       4592
 
PfoI T'CCnGG_A

Cuts at:   1145    1145 
   Size:       4592
 
PmeI GTTT'AAAC

Cuts at:   1591    1591 
   Size:       4592
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    622     654    3731    3763     622 
   Size:         32    3077      32    1451

  Fragments arranged by size: 

                3077    1451      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    129    1316     129 
   Size:       1187    3405
 
PshAI GACnn'nnGTC

Cuts at:    793     793 
   Size:       4592
 
PstI C_TGCA'G

Cuts at:   1639    2324    1639 
   Size:        685    3907
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4592
 
PvuII CAG'CTG

Cuts at:   2017    2377    2017 
   Size:        360    4232
 
RsrII CG'GwC_CG

Cuts at:   2787    2787 
   Size:       4592
 
SacI G_AGCT'C

Cuts at:   1286    1606    1286 
   Size:        320    4272
 
SacII CC_GC'GG

Cuts at:   1129    1129 
   Size:       4592
 
SalI G'TCGA_C

Cuts at:   1629    1629 
   Size:       4592
 
SapI GCTCTTCn'nnn_

Cuts at:    835    1507    2615    2825     835 
   Size:        672    1108     210    2602

  Fragments arranged by size: 

                2602    1108     672     210
 
SbfI CC_TGCA'GG

Cuts at:   1639    1639 
   Size:       4592
 
ScaI AGT'ACT

Cuts at:   4109    4109 
   Size:       4592
 
SfcI C'TryA_G

Cuts at:     33     366    1635    2320    3313    3504      33 
   Size:        333    1269     685     993     191    1121

  Fragments arranged by size: 

                1269    1121     993     685     333     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   1198    1198 
   Size:       4592
 
SfoI GGC'GCC

Cuts at:    768    1350    2024    2272     768 
   Size:        582     674     248    3088

  Fragments arranged by size: 

                3088     674     582     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4592
 
SgrAI Cr'CCGG_yG

Cuts at:   1426    1426 
   Size:       4592
 
SmaI CCC'GGG

Cuts at:    740    1198    1614     740 
   Size:        458     416    3718

  Fragments arranged by size: 

                3718     458     416
 
SmlI C'TyrA_G

Cuts at:    265    1747    2662    3154    3416     265 
   Size:       1482     915     492     262    1441

  Fragments arranged by size: 

                1482    1441     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4592
 
SphI G_CATG'C

Cuts at:   1645    2145    1645 
   Size:        500    4092
 
SspI AAT'ATT

Cuts at:    453     453 
   Size:       4592
 
StuI AGG'CCT

Cuts at:    700     700 
   Size:       4592
 
StyI C'CwwG_G

Cuts at:    107    1213    1726    2080     107 
   Size:       1106     513     354    2619

  Fragments arranged by size: 

                2619    1106     513     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1126    1437    2175    2308    2767    2976    1126 
   Size:        311     738     133     459     209    2742

  Fragments arranged by size: 

                2742     738     459     311     209     133
 
TatI w'GTAC_w

Cuts at:   4107    4107 
   Size:       4592
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1105    1137    4109    4141    1105 
   Size:         32    2972      32    1556

  Fragments arranged by size: 

                2972    1556      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2389    2389 
   Size:       4592
 
XbaI T'CTAG_A

Cuts at:   1623    1623 
   Size:       4592
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2087    2087 
   Size:       4592
 
XmaI C'CCGG_G

Cuts at:    738    1196    1612     738 
   Size:        458     416    3718

  Fragments arranged by size: 

                3718     458     416
 
ZraI GAC'GTC

Cuts at:    795     795 
   Size:       4592
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    FspAI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AhdI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI    BbvCI     BcgI    BfrBI     BmtI   Bpu10I    BsaXI
     BsgI    BsiWI    BsmBI    BsrGI   BssHII  BstZ17I   DraIII    EcoNI
    EcoRV     FseI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PmlI     PsiI   PspOMI    PspXI     PsrI
    SanDI    SexAI    SnaBI     SrfI     SwaI     XhoI     XmnI
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