Restriction map for
pF1KE3008
(Circular) (Six-base) MAPSORT of: pF1KE3008.seq Check: 2776 from: 1 to: 3380
pF1KE3008 3380 bp
With 182 enzymes: SgfI *
September 20, 2013 16:32 ..
AccI GT'mk_AC
Cuts at: 418 418
Size: 3380
Acc65I G'GTAC_C
Cuts at: 396 396
Size: 3380
AclI AA'CG_TT
Cuts at: 3220 3220
Size: 3380
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 1205 1637 2384 2652 154
Size: 1051 432 747 268 882
Fragments arranged by size:
1051 882 747 432 268
AflIII A'CryG_T
Cuts at: 732 1836 2813 732
Size: 1104 977 1299
Fragments arranged by size:
1299 1104 977
AgeI A'CCGG_T
Cuts at: 1768 1768
Size: 3380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 386 386
Size: 3380
AlwNI CAG_nnn'CTG
Cuts at: 685 2252 685
Size: 1567 1813
ApaLI G'TGCA_C
Cuts at: 2150 2150
Size: 3380
ApoI r'AATT_y
Cuts at: 384 2733 2989 384
Size: 2349 256 775
Fragments arranged by size:
2349 775 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3380
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3380
AvaI C'yCGr_G
Cuts at: 238 400 238
Size: 162 3218
BamHI G'GATC_C
Cuts at: 405 405
Size: 3380
BanI G'GyrC_C
Cuts at: 195 396 810 1058 1093 195
Size: 201 414 248 35 2482
Fragments arranged by size:
2482 414 248 201 35
BanII G_rGCy'C
Cuts at: 167 311 394 1424 167
Size: 144 83 1030 2123
Fragments arranged by size:
2123 1030 144 83
BbeI G_GCGC'C
Cuts at: 814 1062 814
Size: 248 3132
BciVI GTATCCnnnnn_n'
Cuts at: 1278 2039 3380 1278
Size: 761 1341 1278
Fragments arranged by size:
1341 1278 761
BclI T'GATC_A
Cuts at: 900 900
Size: 3380
BglII A'GATC_T
Cuts at: 895 2932 895
Size: 2037 1343
BlpI GC'TnA_GC
Cuts at: 492 492
Size: 3380
Bme1580I G_kGCm'C
Cuts at: 1005 1098 2154 1005
Size: 93 1056 2231
Fragments arranged by size:
2231 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 592 673 1008 3152 592
Size: 81 335 2144 820
Fragments arranged by size:
2144 820 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1747 1747
Size: 3380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 163
Size: 3380
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 556 1435 1927 2225 151
Size: 405 879 492 298 1306
Fragments arranged by size:
1306 879 492 405 298
BsaI GGTCTCn'nnnn_
Cuts at: 3084 3084
Size: 3380
BsaAI yAC'GTr
Cuts at: 733 733
Size: 3380
BsaBI GATnn'nnATC
Cuts at: 6 894 2931 6
Size: 888 2037 455
Fragments arranged by size:
2037 888 455
BsaHI Gr'CG_yC
Cuts at: 811 1059 1761 3257 811
Size: 248 702 1496 934
Fragments arranged by size:
1496 934 702 248
BsaWI w'CCGG_w
Cuts at: 156 569 1090 1768 2042 2189 2600 156
Size: 413 521 678 274 147 411 936
Fragments arranged by size:
936 678 521 413 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 361 175
Size: 186 3194
BseYI C'CCAG_C
Cuts at: 186 805 1563 2140 2636 186
Size: 619 758 577 496 930
Fragments arranged by size:
930 758 619 577 496
BsiEI CG_ry'CG
Cuts at: 104 922 968 2176 104
Size: 818 46 1208 1308
Fragments arranged by size:
1308 1208 818 46
BsiHKAI G_wGCw'C
Cuts at: 167 311 394 1172 1362 2154 167
Size: 144 83 778 190 792 1393
Fragments arranged by size:
1393 792 778 190 144 83
Bsp1286I G_dGCh'C
Cuts at: 167 311 394 1005 1098 1172 1362 1424 2154
Size: 144 83 611 93 74 190 62 730
Cuts at: 2154 167
Size: 1393
Fragments arranged by size:
1393 730 611 190 144 93 83 74
62
BspEI T'CCGG_A
Cuts at: 2600 2600
Size: 3380
BspHI T'CATG_A
Cuts at: 2556 2556
Size: 3380
BspMI ACCTGCnnnn'nnnn_
Cuts at: 430 644 946 1327 430
Size: 214 302 381 2483
Fragments arranged by size:
2483 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1672 1726 3210 3375 50
Size: 1622 54 1484 165 55
Fragments arranged by size:
1622 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1292 1292
Size: 3380
BsrFI r'CCGG_y
Cuts at: 1378 1559 1768 1378
Size: 181 209 2990
Fragments arranged by size:
2990 209 181
BssHII G'CGCG_C
Cuts at: 233 233
Size: 3380
BssSI C'ACGA_G
Cuts at: 1651 2009 1651
Size: 358 3022
BstAPI GCAn_nnn'nTGC
Cuts at: 757 757
Size: 3380
BstBI TT'CG_AA
Cuts at: 1741 1741
Size: 3380
BstYI r'GATC_y
Cuts at: 1 405 872 895 1230 1476 2488 2574 2586
Size: 404 467 23 335 246 1012 86 12
Cuts at: 2586 2932 1
Size: 346 449
Fragments arranged by size:
1012 467 449 404 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 226 107
Size: 119 3261
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1330 1511 1674 1330
Size: 181 163 3036
Fragments arranged by size:
3036 181 163
BtsI GCAGTG_nn'
Cuts at: 623 623
Size: 3380
DraI TTT'AAA
Cuts at: 379 379
Size: 3380
DrdI GACnn_nn'nnGTC
Cuts at: 274 1086 1944 274
Size: 812 858 1710
Fragments arranged by size:
1710 858 812
EaeI y'GGCC_r
Cuts at: 317 965 1139 1530 1557 2902 317
Size: 648 174 391 27 1345 795
Fragments arranged by size:
1345 795 648 391 174 27
EagI C'GGCC_G
Cuts at: 965 965
Size: 3380
EarI CTCTTCn'nnn_
Cuts at: 1403 1613 1403
Size: 210 3170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1588 1898 2044 3190 1588
Size: 310 146 1146 1778
Fragments arranged by size:
1778 1146 310 146
EcoICRI GAG'CTC
Cuts at: 165 309 392 165
Size: 144 83 3153
Fragments arranged by size:
3153 144 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 163 1205 1637 2384 2652 154
Size: 9 1042 432 747 268 882
Fragments arranged by size:
1042 882 747 432 268 9
EcoO109I rG'GnC_Cy
Cuts at: 519 519
Size: 3380
EcoRI G'AATT_C
Cuts at: 384 384
Size: 3380
EcoRV GAT'ATC
Cuts at: 252 252
Size: 3380
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1399 1399
Size: 3380
FspI TGC'GCA
Cuts at: 337 1161 337
Size: 824 2556
FspAI rTGC'GCAy
Cuts at: 337 337
Size: 3380
HaeII r_GCGC'y
Cuts at: 814 1062 2084 3063 814
Size: 248 1022 979 1131
Fragments arranged by size:
1131 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1222 1252 1254 1284 1222
Size: 30 2 30 3318
Fragments arranged by size:
3318 30 30 2
HincII GTy'rAC
Cuts at: 419 419
Size: 3380
KasI G'GCGC_C
Cuts at: 810 1058 810
Size: 248 3132
KpnI G_GTAC'C
Cuts at: 400 400
Size: 3380
MluI A'CGCG_T
Cuts at: 2813 2813
Size: 3380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 323 2051 2235 323
Size: 1728 184 1468
Fragments arranged by size:
1728 1468 184
MscI TGG'CCA
Cuts at: 1141 1141
Size: 3380
MslI CAynn'nnrTG
Cuts at: 337 1369 1496 337
Size: 1032 127 2221
Fragments arranged by size:
2221 1032 127
MspA1I CmG'CkG
Cuts at: 201 228 366 491 805 1165 2178 2423 201
Size: 27 138 125 314 360 1013 245 1158
Fragments arranged by size:
1158 1013 360 314 245 138 125 27
NaeI GCC'GGC
Cuts at: 1561 1561
Size: 3380
NarI GG'CG_CC
Cuts at: 811 1059 811
Size: 248 3132
NcoI C'CATG_G
Cuts at: 107 107
Size: 3380
NgoMIV G'CCGG_C
Cuts at: 1559 1559
Size: 3380
NspI r_CATG'y
Cuts at: 336 342 433 933 1840 336
Size: 6 91 500 907 1876
Fragments arranged by size:
1876 907 500 91 6
PciI A'CATG_T
Cuts at: 1836 1836
Size: 3380
PmeI GTTT'AAAC
Cuts at: 379 379
Size: 3380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2519 2551 2519
Size: 32 3348
PstI C_TGCA'G
Cuts at: 365 427 1112 365
Size: 62 685 2633
Fragments arranged by size:
2633 685 62
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3380
PvuII CAG'CTG
Cuts at: 805 1165 805
Size: 360 3020
RsrII CG'GwC_CG
Cuts at: 1575 1575
Size: 3380
SacI G_AGCT'C
Cuts at: 167 311 394 167
Size: 144 83 3153
Fragments arranged by size:
3153 144 83
SacII CC_GC'GG
Cuts at: 229 229
Size: 3380
SalI G'TCGA_C
Cuts at: 417 417
Size: 3380
SapI GCTCTTCn'nnn_
Cuts at: 1403 1613 1403
Size: 210 3170
SbfI CC_TGCA'GG
Cuts at: 427 427
Size: 3380
ScaI AGT'ACT
Cuts at: 2897 2897
Size: 3380
SfcI C'TryA_G
Cuts at: 33 361 423 1108 2101 2292 33
Size: 328 62 685 993 191 1121
Fragments arranged by size:
1121 993 685 328 191 62
SfoI GGC'GCC
Cuts at: 812 1060 812
Size: 248 3132
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3380
SmaI CCC'GGG
Cuts at: 402 402
Size: 3380
SmlI C'TyrA_G
Cuts at: 166 535 1450 1942 2204 166
Size: 369 915 492 262 1342
Fragments arranged by size:
1342 915 492 369 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3380
SphI G_CATG'C
Cuts at: 433 933 433
Size: 500 2880
StyI C'CwwG_G
Cuts at: 107 514 868 107
Size: 407 354 2619
Fragments arranged by size:
2619 407 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 963 1096 1555 1764 963
Size: 133 459 209 2579
Fragments arranged by size:
2579 459 209 133
TatI w'GTAC_w
Cuts at: 2895 2895
Size: 3380
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2897 2929 2897
Size: 32 3348
Tth111I GACn'n_nGTC
Cuts at: 1177 1177
Size: 3380
XbaI T'CTAG_A
Cuts at: 411 411
Size: 3380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 875 875
Size: 3380
XmaI C'CCGG_G
Cuts at: 400 400
Size: 3380
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI FseI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI