(Linear) (Six-base) MAPSORT of: KIEE3008.seq  Check: 2863  from: 1  to: 267



KIEE3008.orf

 With 182 enzymes: SgfI * 

                        September 20, 2013 16:32  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     267 
   Size:         46     221
 
AvaI C'yCGr_G

Cuts at:      0     130     267 
   Size:        130     137
 
BanI G'GyrC_C

Cuts at:      0      87     267 
   Size:         87     180
 
BanII G_rGCy'C

Cuts at:      0      59     203     267 
   Size:         59     144      64

  Fragments arranged by size: 

                 144      64      59
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     267 
   Size:         55     212
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     267 
   Size:         43     224
 
BsaWI w'CCGG_w

Cuts at:      0      48     267 
   Size:         48     219
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67     253     267 
   Size:         67     186      14

  Fragments arranged by size: 

                 186      67      14
 
BseYI C'CCAG_C

Cuts at:      0      78     267 
   Size:         78     189
 
BsiHKAI G_wGCw'C

Cuts at:      0      59     203     267 
   Size:         59     144      64

  Fragments arranged by size: 

                 144      64      59
 
Bsp1286I G_dGCh'C

Cuts at:      0      59     203     267 
   Size:         59     144      64

  Fragments arranged by size: 

                 144      64      59
 
BssHII G'CGCG_C

Cuts at:      0     125     267 
   Size:        125     142
 
BtgI C'CryG_G

Cuts at:      0     118     267 
   Size:        118     149
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     166     267 
   Size:        166     101
 
EaeI y'GGCC_r

Cuts at:      0     209     267 
   Size:        209      58
 
EcoICRI GAG'CTC

Cuts at:      0      57     201     267 
   Size:         57     144      66

  Fragments arranged by size: 

                 144      66      57
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46      55     267 
   Size:         46       9     212

  Fragments arranged by size: 

                 212      46       9
 
EcoRV GAT'ATC

Cuts at:      0     144     267 
   Size:        144     123
 
FspI TGC'GCA

Cuts at:      0     229     267 
   Size:        229      38
 
FspAI rTGC'GCAy

Cuts at:      0     229     267 
   Size:        229      38
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     215     267 
   Size:        215      52
 
MslI CAynn'nnrTG

Cuts at:      0     229     267 
   Size:        229      38
 
MspA1I CmG'CkG

Cuts at:      0      93     120     258     267 
   Size:         93      27     138       9

  Fragments arranged by size: 

                 138      93      27       9
 
NspI r_CATG'y

Cuts at:      0     228     234     267 
   Size:        228       6      33

  Fragments arranged by size: 

                 228      33       6
 
PstI C_TGCA'G

Cuts at:      0     257     267 
   Size:        257      10
 
SacI G_AGCT'C

Cuts at:      0      59     203     267 
   Size:         59     144      64

  Fragments arranged by size: 

                 144      64      59
 
SacII CC_GC'GG

Cuts at:      0     121     267 
   Size:        121     146
 
SfcI C'TryA_G

Cuts at:      0     253     267 
   Size:        253      14
 
SmlI C'TyrA_G

Cuts at:      0      58     267 
   Size:         58     209
 

 Enzymes that do cut:

     AcuI     AvaI     BanI    BanII     BpmI    BpuEI    BsaWI    BseRI
    BseYI  BsiHKAI Bsp1286I   BssHII     BtgI     DrdI     EaeI  EcoICRI
  Eco57MI    EcoRV     FspI    FspAI     MmeI     MslI   MspA1I     NspI
     PstI     SacI    SacII     SfcI     SmlI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EagI     EarI
     EciI    EcoNI EcoO109I    EcoRI     FalI     FseI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI