Restriction map for pF1KE9678
 (Linear) (Six-base) MAPSORT of: KIEE9678.seq  Check: 5886  from: 1  to: 4251



KIEE9678.orf

 With 182 enzymes: SgfI * 

                        April 12, 2016 09:51  ..

AccI GT'mk_AC

Cuts at:      0    3344    3560    4251 
   Size:       3344     216     691

  Fragments arranged by size: 

                3344     691     216
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     201     752    1186    2554    3821    3896    4251 
   Size:        110      91     551     434    1368    1267      75     355

  Fragments arranged by size: 

                1368    1267     551     434     355     110      91      75
            
 
AflII C'TTAA_G

Cuts at:      0    4228    4251 
   Size:       4228      23
 
AflIII A'CryG_T

Cuts at:      0    2717    3303    4087    4251 
   Size:       2717     586     784     164

  Fragments arranged by size: 

                2717     784     586     164
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1817    4251 
   Size:       1817    2434
 
AleI CACnn'nnGTG

Cuts at:      0    1585    4251 
   Size:       1585    2666
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2646    4251 
   Size:       2646    1605
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1056    4251 
   Size:       1056    3195
 
ApoI r'AATT_y

Cuts at:      0      91     418     819     868    1341    1359    1380    1896 
   Size:         91     327     401      49     473      18      21     516

Cuts at:   1896    1984    3579    4251 
   Size:         88    1595     672

  Fragments arranged by size: 

                1595     672     516     473     401     327      91      88
                  49      21      18
 
AseI AT'TA_AT

Cuts at:      0    1144    2804    4251 
   Size:       1144    1660    1447

  Fragments arranged by size: 

                1660    1447    1144
 
AvaI C'yCGr_G

Cuts at:      0     392    4251 
   Size:        392    3859
 
AvrII C'CTAG_G

Cuts at:      0    1419    4251 
   Size:       1419    2832
 
BamHI G'GATC_C

Cuts at:      0    4132    4251 
   Size:       4132     119
 
BanII G_rGCy'C

Cuts at:      0    2738    3944    4251 
   Size:       2738    1206     307

  Fragments arranged by size: 

                2738    1206     307
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     212    1258    1709    1813    2054    3476    3809    3884 
   Size:        212    1046     451     104     241    1422     333      75

Cuts at:   3884    4251 
   Size:        367

  Fragments arranged by size: 

                1422    1046     451     367     333     241     212     104
                  75
 
BbvCI CC'TCA_GC

Cuts at:      0    1803    3935    4251 
   Size:       1803    2132     316

  Fragments arranged by size: 

                2132    1803     316
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     390    4251 
   Size:        390    3861
 
BfrBI ATG'CAT

Cuts at:      0    1713    2890    4243    4251 
   Size:       1713    1177    1353       8

  Fragments arranged by size: 

                1713    1353    1177       8
 
BglII A'GATC_T

Cuts at:      0    1017    2306    4251 
   Size:       1017    1289    1945

  Fragments arranged by size: 

                1945    1289    1017
 
BmgBI CAC'GTC

Cuts at:      0    2871    4251 
   Size:       2871    1380
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1521    1707    4251 
   Size:       1521     186    2544

  Fragments arranged by size: 

                2544    1521     186
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2752    4251 
   Size:       2752    1499
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     263     896    2093    2236    2282    2359    2673    4104 
   Size:        263     633    1197     143      46      77     314    1431

Cuts at:   4104    4251 
   Size:        147

  Fragments arranged by size: 

                1431    1197     633     314     263     147     143      77
                  46
 
Bpu10I CC'TnA_GC

Cuts at:      0     755    1803    3935    4251 
   Size:        755    1048    2132     316

  Fragments arranged by size: 

                2132    1048     755     316
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391    2166    3721    4142    4251 
   Size:        391    1775    1555     421     109

  Fragments arranged by size: 

                1775    1555     421     391     109
 
BsaBI GATnn'nnATC

Cuts at:      0     312     709    4251 
   Size:        312     397    3542

  Fragments arranged by size: 

                3542     397     312
 
BsaWI w'CCGG_w

Cuts at:      0    2472    2865    4251 
   Size:       2472     393    1386

  Fragments arranged by size: 

                2472    1386     393
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2671    2701    3351    3381    4251 
   Size:       2671      30     650      30     870

  Fragments arranged by size: 

                2671     870     650      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     624    3952    4053    4251 
   Size:        624    3328     101     198

  Fragments arranged by size: 

                3328     624     198     101
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2018    2809    4251 
   Size:       2018     791    1442

  Fragments arranged by size: 

                2018    1442     791
 
BsiHKAI G_wGCw'C

Cuts at:      0    1133    3944    4251 
   Size:       1133    2811     307

  Fragments arranged by size: 

                2811    1133     307
 
BsmI GAATG_Cn'

Cuts at:      0    3109    4243    4251 
   Size:       3109    1134       8

  Fragments arranged by size: 

                3109    1134       8
 
Bsp1286I G_dGCh'C

Cuts at:      0    1133    2738    3944    4251 
   Size:       1133    1605    1206     307

  Fragments arranged by size: 

                1605    1206    1133     307
 
BspHI T'CATG_A

Cuts at:      0    2985    4251 
   Size:       2985    1266
 
BsrDI GCAATG_nn'

Cuts at:      0     725    4251 
   Size:        725    3526
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2929    4251 
   Size:       2929    1322
 
BstYI r'GATC_y

Cuts at:      0    1017    2306    3400    4132    4251 
   Size:       1017    1289    1094     732     119

  Fragments arranged by size: 

                1289    1094    1017     732     119
 
Bsu36I CC'TnA_GG

Cuts at:      0    2549    3770    4251 
   Size:       2549    1221     481

  Fragments arranged by size: 

                2549    1221     481
 
BtgI C'CryG_G

Cuts at:      0    2708    4251 
   Size:       2708    1543
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     713    3851    4251 
   Size:        713    3138     400

  Fragments arranged by size: 

                3138     713     400
 
BtsI GCAGTG_nn'

Cuts at:      0     542    1580    2035    2961    3594    4251 
   Size:        542    1038     455     926     633     657

  Fragments arranged by size: 

                1038     926     657     633     542     455
 
ClaI AT'CG_AT

Cuts at:      0     713    4251 
   Size:        713    3538
 
DraI TTT'AAA

Cuts at:      0     111     270     582    3438    3554    4251 
   Size:        111     159     312    2856     116     697

  Fragments arranged by size: 

                2856     697     312     159     116     111
 
DraIII CAC_nnn'GTG

Cuts at:      0    2395    4251 
   Size:       2395    1856
 
EaeI y'GGCC_r

Cuts at:      0    1771    2489    4251 
   Size:       1771     718    1762

  Fragments arranged by size: 

                1771    1762     718
 
EarI CTCTTCn'nnn_

Cuts at:      0     948    2937    3629    3997    4251 
   Size:        948    1989     692     368     254

  Fragments arranged by size: 

                1989     948     692     368     254
 
EcoICRI GAG'CTC

Cuts at:      0    3942    4251 
   Size:       3942     309
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     201     263     752     896    1186    2093    2236 
   Size:        110      91      62     489     144     290     907     143

Cuts at:   2236    2282    2359    2554    2673    3821    3896    4104    4251 
   Size:         46      77     195     119    1148      75     208     147

  Fragments arranged by size: 

                1148     907     489     290     208     195     147     144
                 143     119     110      91      77      75      62      46
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    4026    4251 
   Size:       4026     225
 
EcoO109I rG'GnC_Cy

Cuts at:      0     969    3310    3916    4251 
   Size:        969    2341     606     335

  Fragments arranged by size: 

                2341     969     606     335
 
EcoRI G'AATT_C

Cuts at:      0    1341    4251 
   Size:       1341    2910
 
EcoRV GAT'ATC

Cuts at:      0    4100    4251 
   Size:       4100     151
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     282     591     607    3579    4251 
   Size:        282     309      16    2972     672

  Fragments arranged by size: 

                2972     672     309     282      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1009    1041    1803    1835    2141    2173    4182    4214 
   Size:       1009      32     762      32     306      32    2009      32

Cuts at:   4214    4251 
   Size:         37

  Fragments arranged by size: 

                2009    1009     762     306      37      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    3345    4251 
   Size:       3345     906
 
HindIII A'AGCT_T

Cuts at:      0    3742    4251 
   Size:       3742     509
 
MfeI C'AATT_G

Cuts at:      0    2837    4251 
   Size:       2837    1414
 
MslI CAynn'nnrTG

Cuts at:      0     743    1585    2660    3086    3176    4251 
   Size:        743     842    1075     426      90    1075

  Fragments arranged by size: 

                1075    1075     842     743     426      90
 
MspA1I CmG'CkG

Cuts at:      0    1209    1744    2532    3874    3934    4251 
   Size:       1209     535     788    1342      60     317

  Fragments arranged by size: 

                1342    1209     788     535     317      60
 
NcoI C'CATG_G

Cuts at:      0    2708    4251 
   Size:       2708    1543
 
NdeI CA'TA_TG

Cuts at:      0    2661    4239    4251 
   Size:       2661    1578      12

  Fragments arranged by size: 

                2661    1578      12
 
NsiI A_TGCA'T

Cuts at:      0    1715    2892    4245    4251 
   Size:       1715    1177    1353       6

  Fragments arranged by size: 

                1715    1353    1177       6
 
NspI r_CATG'y

Cuts at:      0    3307    4091    4251 
   Size:       3307     784     160

  Fragments arranged by size: 

                3307     784     160
 
PasI CC'CwG_GG

Cuts at:      0    2504    3889    4251 
   Size:       2504    1385     362

  Fragments arranged by size: 

                2504    1385     362
 
PciI A'CATG_T

Cuts at:      0    3303    4087    4251 
   Size:       3303     784     164

  Fragments arranged by size: 

                3303     784     164
 
PpuMI rG'GwC_Cy

Cuts at:      0     969    3310    4251 
   Size:        969    2341     941

  Fragments arranged by size: 

                2341     969     941
 
PsiI TTA'TAA

Cuts at:      0    3013    3215    4251 
   Size:       3013     202    1036

  Fragments arranged by size: 

                3013    1036     202
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    3245    3277    4251 
   Size:       3245      32     974

  Fragments arranged by size: 

                3245     974      32
 
PvuII CAG'CTG

Cuts at:      0    1744    2532    3874    4251 
   Size:       1744     788    1342     377

  Fragments arranged by size: 

                1744    1342     788     377
 
SacI G_AGCT'C

Cuts at:      0    3944    4251 
   Size:       3944     307
 
SalI G'TCGA_C

Cuts at:      0    3343    4251 
   Size:       3343     908
 
SfcI C'TryA_G

Cuts at:      0      25    1334    4251 
   Size:         25    1309    2917

  Fragments arranged by size: 

                2917    1309      25
 
SmaI CCC'GGG

Cuts at:      0     394    4251 
   Size:        394    3857
 
SmlI C'TyrA_G

Cuts at:      0     406    2145    3736    4157    4228    4251 
   Size:        406    1739    1591     421      71      23

  Fragments arranged by size: 

                1739    1591     421     406      71      23
 
SspI AAT'ATT

Cuts at:      0    2244    3717    4251 
   Size:       2244    1473     534

  Fragments arranged by size: 

                2244    1473     534
 
StuI AGG'CCT

Cuts at:      0    1293    1467    4251 
   Size:       1293     174    2784

  Fragments arranged by size: 

                2784    1293     174
 
StyI C'CwwG_G

Cuts at:      0    1419    2186    2708    4064    4251 
   Size:       1419     767     522    1356     187

  Fragments arranged by size: 

                1419    1356     767     522     187
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2672    3188    4251 
   Size:       2672     516    1063

  Fragments arranged by size: 

                2672    1063     516
 
TatI w'GTAC_w

Cuts at:      0    3537    4251 
   Size:       3537     714
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2484    2516    3351    3383    4251 
   Size:       2484      32     835      32     868

  Fragments arranged by size: 

                2484     868     835      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    4182    4251 
   Size:       4182      69
 
XmaI C'CCGG_G

Cuts at:      0     392    4251 
   Size:        392    3859
 
XmnI GAAnn'nnTTC

Cuts at:      0    1199    4251 
   Size:       1199    3052
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AhdI     AleI     AlfI    AlwNI
     ApoI     AseI     AvaI    AvrII    BamHI    BanII     BbsI    BbvCI
    BciVI    BfrBI    BglII    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI    BsaBI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BsrDI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI     DraI   DraIII     EaeI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI    Hin4I   HincII  HindIII
     MfeI     MslI   MspA1I     NcoI     NdeI     NsiI     NspI     PasI
     PciI    PpuMI     PsiI     PsrI    PvuII     SacI     SalI     SfcI
     SmaI     SmlI     SspI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AloI     ApaI
    ApaLI     AscI    AsiSI     BaeI     BanI     BbeI     BcgI     BclI
     BglI     BlpI Bme1580I     BmtI     BsaI    BsaAI    BsaHI    BseYI
    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I    CspCI     DrdI
     EagI     EciI     FseI     FspI    FspAI    HaeII     HpaI     KasI
     KpnI     MluI     MmeI     MscI     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     PacI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI   PspOMI    PspXI     PstI     PvuI    RsrII    SacII    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SwaI  Tth111I     XbaI     XhoI
     ZraI
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