Restriction map for pF1KB5968
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

Acc65I      AccIII      AclI        AgeI        AhdI        
Alw44I      AlwI        AlwNI       Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BcnI        BglI        BlnI        BsaAI       
BsaBI       BsaI        BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsmBI       BsmFI       Bsp1407I    
BspDI       BspEI       BspHI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstEII      BstPI       BstXI       BstZ17I     Cfr10I      
ClaI        CpoI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRI       EcoT22I     FseI        FspI        HaeII       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MluI        MluNI       
MroI        MscI        NaeI        NarI        NciI        
NdeI        NgoMIV      NotI        NruI        NsiI        
PacI        PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacII       SalI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
SphI        SspBI       SspI        StuI        SwaI        
Tth111I     Van91I      XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII        1264  GACGTC               GACGT^C
AccI         1521  GTCTAC               GT^[AC][GT]AC
AccII         607  CGCG                 CG^CG
AflII        1527  CTTAAG               C^TTAAG
ApaI           11  GGGCCC               GGGCC^C
Asp700I       304  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         979  GAGCTC               G[AT]GC[AT]^C
AvaI          515  CTCGAG               C^[CT]CG[GA]G
BclI         1149  TGATCA               T^GATCA
BfrI         1527  CTTAAG               C^TTAAG
BglII         320  AGATCT               A^GATCT
BlpI          634  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      634  GCTGAGC              GC^T[ACGT]AGC
BsaWI         587  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       979  GAGCTC               G[AT]GC[AT]^C
BsmI          291  GAATGC               GAATGC(1/-1)
BsoBI         515  CTCGAG               C^[CT]CG[GA]G
BspLU11I      364  ACATGT               A^CATGT
BstBI          77  TTCGAA               TT^CGAA
BstUI         607  CGCG                 CG^CG
BstYI         320  AGATCT               [GA]^GATC[CT]
CelII         634  GCTGAGC              GC^T[ACGT]AGC
CfoI          606  GCGC                 GCG^C
DraI         1570  TTTAAA               TTT^AAA
DrdI          742  GACTCCGCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV         802  GATATC               GAT^ATC
EcoT14I       905  CCATGG               C^C[AT][AT]GG
FauI         1044  GCGGG                CCCGC(4/6)
FbaI         1149  TGATCA               T^GATCA
HapII         588  CCGG                 C^CGG
HhaI          606  GCGC                 GCG^C
HinP1I        606  GCGC                 G^CGC
HpaII         588  CCGG                 C^CGG
MfeI         1060  CAATTG               C^AATTG
MflI          320  AGATCT               [GA]^GATC[CT]
MslI          349  CATTATGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          588  CCGG                 C^CGG
MunI         1060  CAATTG               C^AATTG
MvnI          607  CGCG                 CG^CG
NcoI          905  CCATGG               C^CATGG
NheI         1449  GCTAGC               G^CTAGC
NspI          364  ACATGT               [GA]CATG^[CT]
NspV           77  TTCGAA               TT^CGAA
PaeR7I        515  CTCGAG               C^TCGAG
PstI          116  CTGCAG               CTGCA^G
SacI          979  GAGCTC               GAGCT^C
SbfI          115  CCTGCAGG             CCTGCA^GG
SfuI           77  TTCGAA               TT^CGAA
Sse8387I      115  CCTGCAGG             CCTGCA^GG
StyI          905  CCATGG               C^C[AT][AT]GG
XbaI         1179  TCTAGA               T^CTAGA
XcmI          615  CCAGCAGCAGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          515  CTCGAG               C^TCGAG
XhoII         320  AGATCT               [GA]^GATC[CT]
XmnI          304  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AflIII        364  ACATGT               A^C[GA][CT]GT
             1461  ACACGT              
AocI          724  CCTGAGG              CC^T[ACGT]AGG
             1277  CCTTAGG             
BanII          11  GGGCCC               G[GA]GC[CT]^C
              979  GAGCTC              
BciVI         283  GGATAC               GTATCC(6/5)
              176  GTATCC              
BfaI         1180  CTAG                 C^TAG
             1450  CTAG                
BsiYI         722  CCCCTGAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              844  CCAGCATCAGG         
BslI          722  CCCCTGAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              844  CCAGCATCAGG         
BsrDI         407  GCAATG               GCAATG(2/0)
             1651  GCAATG              
Bsu36I        724  CCTGAGG              CC^T[ACGT]AGG
             1277  CCTTAGG             
Cfr13I         11  GGGCC                G^G[ACGT]CC
               12  GGCCC               
DraII          10  GGGGCCC              [GA]G^G[ACGT]CC[CT]
               11  GGGCCCC             
DsaI           52  CCGTGG               C^C[GA][CT]GG
              905  CCATGG              
EaeI          534  TGGCCG               [CT]^GGCC[GA]
              665  CGGCCA              
EarI         1454  CTCTTC               CTCTTC(1/4)
              976  GAAGAG              
Eco81I        724  CCTGAGG              CC^T[ACGT]AGG
             1277  CCTTAGG             
EcoO109I       10  GGGGCCC              [GA]G^G[ACGT]CC[CT]
               11  GGGCCCC             
HgaI          645  GACGC                GACGC(5/10)
              212  GCGTC               
Ksp632I      1454  CTCTTC               CTCTTC(1/4)
              976  GAAGAG              
MaeI         1180  CTAG                 C^TAG
             1450  CTAG                
PleI          742  GACTC                GAGTC(4/5)
             1216  GACTC               
SapI          976  GAAGAGC              GCTCTTC(1/4)
             1453  GCTCTTC             
Sau96I         11  GGGCC                G^G[ACGT]CC
               12  GGCCC               
SfcI          116  CTGCAG               C^T[GA][CT]AG
             1468  CTACAG              
Tsp45I         26  GTGAC                ^GT[GC]AC
              833  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
ApoI        BbsI        BbvI        BmyI        BpmI        
BpuAI       BsaHI       BsaJI       BsmAI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       DdeI        DpnII       EcoRII      Fnu4HI      
FokI        HaeIII      Hin1I       HinfI       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
ScrFI       SfaNI       SmlI        TaqI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       TthHB8I     
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