################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflIII AgeI Alw44I AlwI AlwNI AocI ApaI ApaLI AscI Asp718I AspHI AspI AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BlnI BpmI BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraII DraIII EaeI EagI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoT14I EcoT22I FauI FbaI FseI FspI HgaI HincII HindII HpaI HphI KpnI MaeII MamI MfeI MluI MluNI MroI MscI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccII 7 CGCG CG^CG AcyI 37 GGCGCC G[GA]^CG[CT]C AfaI 137 GTAC GT^AC AflII 901 CTTAAG C^TTAAG AhdI 508 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 557 AGCGCT AGC^GCT AseI 772 ATTAAT AT^TAAT AsnI 772 ATTAAT AT^TAAT AspEI 508 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 57 CTCGGG C^[CT]CG[GA]G BanI 37 GGCGCC G^G[CT][GA]CC BbeI 37 GGCGCC GGCGC^C BcnI 81 CCCGG CC^[GC]GG BfrI 901 CTTAAG C^TTAAG BglI 29 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1238 AGATCT A^GATCT BlpI 51 GCTCAGC GC^T[ACGT]AGC BmyI 1224 GGGCAC G[AGT]GC[ACT]^C Bpu1102I 51 GCTCAGC GC^T[ACGT]AGC BsaHI 37 GGCGCC G[GA]^CG[CT]C BseRI 70 GAGGAG GAGGAG(10/8) BsmI 736 GCATTC GAATGC(1/-1) BsoBI 57 CTCGGG C^[CT]CG[GA]G Bsp1286I 1224 GGGCAC G[AGT]GC[ACT]^C BspHI 1044 TCATGA T^CATGA BstUI 7 CGCG CG^CG BstYI 1238 AGATCT [GA]^GATC[CT] CelII 51 GCTCAGC GC^T[ACGT]AGC DpnII 1239 GATC ^GATC DrdI 292 GACTGTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 508 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 557 AGCGCT AGC^GCT EcoRV 794 GATATC GAT^ATC HapII 82 CCGG C^CGG Hin1I 37 GGCGCC G[GA]^CG[CT]C HindIII 1204 AAGCTT A^AGCTT HpaII 82 CCGG C^CGG KasI 37 GGCGCC G^GCGCC KspI 6 CCGCGG CCGC^GG MboI 1239 GATC ^GATC MflI 1238 AGATCT [GA]^GATC[CT] MspI 82 CCGG C^CGG MvnI 7 CGCG CG^CG MwoI 29 GCCTCCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NarI 37 GGCGCC GG^CGCC NciI 81 CCCGG CC^[GC]GG NdeII 1239 GATC ^GATC NspI 762 ACATGC [GA]CATG^[CT] PflMI 1273 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1212 GACTC GAGTC(4/5) PshBI 772 ATTAAT AT^TAAT RcaI 1044 TCATGA T^CATGA RsaI 137 GTAC GT^AC SacII 6 CCGCGG CCGC^GG Sau3AI 1239 GATC ^GATC SexAI 811 ACCTGGT A^CC[AT]GGT SfaNI 583 GATGC GCATC(5/9) SfoI 37 GGCGCC GGC^GCC SmlI 901 CTTAAG C^T[CT][GA]AG Tsp45I 212 GTGAC ^GT[GC]AC Van91I 1273 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1238 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BbvI 75 GCAGC GCAGC(8/12) 48 GCTGC BfaI 709 CTAG C^TAG 1286 CTAG BsaI 808 GAGACC GGTCTC(1/5) 715 GGTCTC BstXI 111 CCAGGTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 580 CCAGATGCTTGG Cac8I 346 GCGAGC GC[ACGT]^[ACGT]GC 766 GCAAGC DraI 651 TTTAAA TTT^AAA 1183 TTTAAA DsaI 6 CCGCGG C^C[GA][CT]GG 63 CCACGG EarI 243 GAAGAG CTCTTC(1/4) 685 GAAGAG EcoRI 1080 GAATTC G^AATTC 1294 GAATTC Ksp632I 243 GAAGAG CTCTTC(1/4) 685 GAAGAG MaeI 709 CTAG C^TAG 1286 CTAG MspA1I 6 CCGCGG C[AC]G^C[GT]G 89 CAGCGG NlaIV 37 GGCGCC GG[ACGT]^[ACGT]CC 576 GGGACC SfcI 174 CTACAG C^T[GA][CT]AG 741 CTACAG SspI 1150 AATATT AAT^ATT 1396 AATATT TaqI 965 TCGA T^CGA 1134 TCGA TseI 48 GCTGC G^C[AT]GC 75 GCAGC TthHB8I 965 TCGA T^CGA 1134 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AcsI AluI ApoI Asp700I AvaII BsaJI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI CfoI Cfr13I DdeI EcoRII Fnu4HI FokI HaeII HaeIII HhaI HinP1I HinfI ItaI MaeIII MboII MnlI MseI MslI MvaI NlaIII Sau96I ScrFI TfiI Tru9I Tsp509I TspRI XmnI