(Circular) (Six-base) MAPSORT of: pF1KE2351.seq Check: 1317 from: 1 to: 4517 pF1KE2351 4517 bp With 182 enzymes: SgfI * June 2, 2011 17:00 .. AccI GT'mk_AC Cuts at: 1555 1555 Size: 4517 Acc65I G'GTAC_C Cuts at: 1533 1533 Size: 4517 AclI AA'CG_TT Cuts at: 4357 4357 Size: 4517 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 370 491 550 575 650 1007 1300 1363 1470 Size: 121 59 25 75 357 293 63 107 Cuts at: 1470 2342 2774 3521 3789 370 Size: 872 432 747 268 1098 Fragments arranged by size: 1098 872 747 432 357 293 268 121 107 75 63 59 25 AfeI AGC'GCT Cuts at: 667 667 Size: 4517 AflII C'TTAA_G Cuts at: 1009 1009 Size: 4517 AflIII A'CryG_T Cuts at: 1869 2973 3950 1869 Size: 1104 977 2436 Fragments arranged by size: 2436 1104 977 AgeI A'CCGG_T Cuts at: 2905 2905 Size: 4517 AhdI GACnn_n'nnGTC Cuts at: 621 621 Size: 4517 AleI CACnn'nnGTG Cuts at: 207 207 Size: 4517 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 225 901 911 225 Size: 676 10 3831 Fragments arranged by size: 3831 676 10 AlwNI CAG_nnn'CTG Cuts at: 1822 3389 1822 Size: 1567 2950 ApaLI G'TGCA_C Cuts at: 3287 3287 Size: 4517 ApoI r'AATT_y Cuts at: 955 977 1109 1188 1402 1454 1521 3870 4126 Size: 22 132 79 214 52 67 2349 256 Cuts at: 4126 955 Size: 1346 Fragments arranged by size: 2349 1346 256 214 132 79 67 52 22 AseI AT'TA_AT Cuts at: 20 881 20 Size: 861 3656 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4517 AvaI C'yCGr_G Cuts at: 165 1537 165 Size: 1372 3145 BamHI G'GATC_C Cuts at: 1542 1542 Size: 4517 BanI G'GyrC_C Cuts at: 145 1533 1947 2195 2230 145 Size: 1388 414 248 35 2432 Fragments arranged by size: 2432 1388 414 248 35 BanII G_rGCy'C Cuts at: 1531 2561 1531 Size: 1030 3487 BbeI G_GCGC'C Cuts at: 149 1951 2199 149 Size: 1802 248 2467 Fragments arranged by size: 2467 1802 248 BciVI GTATCCnnnnn_n' Cuts at: 2415 3176 4517 2415 Size: 761 1341 2415 Fragments arranged by size: 2415 1341 761 BclI T'GATC_A Cuts at: 2037 2037 Size: 4517 BglI GCCn_nnn'nGGC Cuts at: 143 143 Size: 4517 BglII A'GATC_T Cuts at: 1346 2032 4069 1346 Size: 686 2037 1794 Fragments arranged by size: 2037 1794 686 BlpI GC'TnA_GC Cuts at: 160 1629 160 Size: 1469 3048 Bme1580I G_kGCm'C Cuts at: 1336 2142 2235 3291 1336 Size: 806 93 1056 2562 Fragments arranged by size: 2562 1056 806 93 BmrI ACTGGGnnnn_n' Cuts at: 1729 1810 2145 4289 1729 Size: 81 335 2144 1957 Fragments arranged by size: 2144 1957 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2884 2884 Size: 4517 Bpu10I CC'TnA_GC Cuts at: 662 662 Size: 4517 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1693 2572 3064 3362 1693 Size: 879 492 298 2848 Fragments arranged by size: 2848 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 829 910 4221 829 Size: 81 3311 1125 Fragments arranged by size: 3311 1125 81 BsaAI yAC'GTr Cuts at: 1870 1870 Size: 4517 BsaBI GATnn'nnATC Cuts at: 6 2031 4068 6 Size: 2025 2037 455 Fragments arranged by size: 2037 2025 455 BsaHI Gr'CG_yC Cuts at: 146 1948 2196 2898 4394 146 Size: 1802 248 702 1496 269 Fragments arranged by size: 1802 1496 702 269 248 BsaWI w'CCGG_w Cuts at: 1706 2227 2905 3179 3326 3737 1706 Size: 521 678 274 147 411 2486 Fragments arranged by size: 2486 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 193 193 Size: 4517 BseYI C'CCAG_C Cuts at: 119 1201 1942 2700 3277 3773 119 Size: 1082 741 758 577 496 863 Fragments arranged by size: 1082 863 758 741 577 496 BsiEI CG_ry'CG Cuts at: 104 2059 2105 3313 104 Size: 1955 46 1208 1308 Fragments arranged by size: 1955 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1531 2309 2499 3291 1531 Size: 778 190 792 2757 Fragments arranged by size: 2757 792 778 190 BsmI GAATG_Cn' Cuts at: 844 844 Size: 4517 Bsp1286I G_dGCh'C Cuts at: 1336 1531 2142 2235 2309 2499 2561 3291 1336 Size: 195 611 93 74 190 62 730 2562 Fragments arranged by size: 2562 730 611 195 190 93 74 62 BspEI T'CCGG_A Cuts at: 3737 3737 Size: 4517 BspHI T'CATG_A Cuts at: 1152 3693 1152 Size: 2541 1976 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1567 1781 2083 2464 1567 Size: 214 302 381 3620 Fragments arranged by size: 3620 381 302 214 BsrBI CCG'CTC Cuts at: 50 2809 2863 4347 4512 50 Size: 2759 54 1484 165 55 Fragments arranged by size: 2759 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2429 2429 Size: 4517 BsrFI r'CCGG_y Cuts at: 2515 2696 2905 2515 Size: 181 209 4127 Fragments arranged by size: 4127 209 181 BssSI C'ACGA_G Cuts at: 2788 3146 2788 Size: 358 4159 BstAPI GCAn_nnn'nTGC Cuts at: 1894 1894 Size: 4517 BstBI TT'CG_AA Cuts at: 2878 2878 Size: 4517 BstXI CCAn_nnnn'nTGG Cuts at: 226 695 226 Size: 469 4048 BstYI r'GATC_y Cuts at: 1 1346 1542 2009 2032 2367 2613 3625 3711 Size: 1345 196 467 23 335 246 1012 86 Cuts at: 3711 3723 4069 1 Size: 12 346 449 Fragments arranged by size: 1345 1012 467 449 346 335 246 196 86 23 12 BtgI C'CryG_G Cuts at: 107 114 171 107 Size: 7 57 4453 Fragments arranged by size: 4453 57 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2467 2648 2811 2467 Size: 181 163 4173 Fragments arranged by size: 4173 181 163 BtsI GCAGTG_nn' Cuts at: 530 1334 1760 530 Size: 804 426 3287 Fragments arranged by size: 3287 804 426 DraI TTT'AAA Cuts at: 761 1293 1516 761 Size: 532 223 3762 Fragments arranged by size: 3762 532 223 DrdI GACnn_nn'nnGTC Cuts at: 406 2223 3081 406 Size: 1817 858 1842 Fragments arranged by size: 1842 1817 858 EaeI y'GGCC_r Cuts at: 2102 2276 2667 2694 4039 2102 Size: 174 391 27 1345 2580 Fragments arranged by size: 2580 1345 391 174 27 EagI C'GGCC_G Cuts at: 2102 2102 Size: 4517 EarI CTCTTCn'nnn_ Cuts at: 346 788 2540 2750 346 Size: 442 1752 210 2113 Fragments arranged by size: 2113 1752 442 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2725 3035 3181 4327 2725 Size: 310 146 1146 2915 Fragments arranged by size: 2915 1146 310 146 EcoICRI GAG'CTC Cuts at: 1529 1529 Size: 4517 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 370 491 550 575 650 1007 1300 1363 1470 Size: 121 59 25 75 357 293 63 107 Cuts at: 1470 2342 2774 3521 3789 370 Size: 872 432 747 268 1098 Fragments arranged by size: 1098 872 747 432 357 293 268 121 107 75 63 59 25 EcoO109I rG'GnC_Cy Cuts at: 1656 1656 Size: 4517 EcoRI G'AATT_C Cuts at: 1188 1402 1521 1188 Size: 214 119 4184 Fragments arranged by size: 4184 214 119 EcoRV GAT'ATC Cuts at: 904 904 Size: 4517 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1254 2536 1254 Size: 1282 3235 FspI TGC'GCA Cuts at: 2298 2298 Size: 4517 HaeII r_GCGC'y Cuts at: 149 669 1080 1951 2199 3221 4200 149 Size: 520 411 871 248 1022 979 466 Fragments arranged by size: 1022 979 871 520 466 411 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 345 377 607 639 2359 2389 2391 2421 345 Size: 32 230 32 1720 30 2 30 2441 Fragments arranged by size: 2441 1720 230 32 32 30 30 2 HincII GTy'rAC Cuts at: 1556 1556 Size: 4517 HindIII A'AGCT_T Cuts at: 1312 1312 Size: 4517 KasI G'GCGC_C Cuts at: 145 1947 2195 145 Size: 1802 248 2467 Fragments arranged by size: 2467 1802 248 KpnI G_GTAC'C Cuts at: 1537 1537 Size: 4517 MluI A'CGCG_T Cuts at: 3950 3950 Size: 4517 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3188 3372 3188 Size: 184 4333 MscI TGG'CCA Cuts at: 2278 2278 Size: 4517 MslI CAynn'nnrTG Cuts at: 207 883 893 1181 2506 2633 207 Size: 676 10 288 1325 127 2091 Fragments arranged by size: 2091 1325 676 288 127 10 MspA1I CmG'CkG Cuts at: 116 199 1628 1942 2302 3315 3560 116 Size: 83 1429 314 360 1013 245 1073 Fragments arranged by size: 1429 1073 1013 360 314 245 83 NaeI GCC'GGC Cuts at: 2698 2698 Size: 4517 NarI GG'CG_CC Cuts at: 146 1948 2196 146 Size: 1802 248 2467 Fragments arranged by size: 2467 1802 248 NcoI C'CATG_G Cuts at: 107 107 Size: 4517 NgoMIV G'CCGG_C Cuts at: 2696 2696 Size: 4517 NspI r_CATG'y Cuts at: 874 1570 2070 2977 874 Size: 696 500 907 2414 Fragments arranged by size: 2414 907 696 500 PciI A'CATG_T Cuts at: 2973 2973 Size: 4517 PflMI CCAn_nnn'nTGG Cuts at: 1387 1387 Size: 4517 PmeI GTTT'AAAC Cuts at: 1516 1516 Size: 4517 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3656 3688 3656 Size: 32 4485 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 790 822 790 Size: 32 4485 PstI C_TGCA'G Cuts at: 1564 2249 1564 Size: 685 3832 PvuI CG_AT'CG Cuts at: 104 104 Size: 4517 PvuII CAG'CTG Cuts at: 1942 2302 1942 Size: 360 4157 RsrII CG'GwC_CG Cuts at: 2712 2712 Size: 4517 SacI G_AGCT'C Cuts at: 1531 1531 Size: 4517 SacII CC_GC'GG Cuts at: 117 117 Size: 4517 SalI G'TCGA_C Cuts at: 1554 1554 Size: 4517 SapI GCTCTTCn'nnn_ Cuts at: 2540 2750 2540 Size: 210 4307 SbfI CC_TGCA'GG Cuts at: 1564 1564 Size: 4517 ScaI AGT'ACT Cuts at: 4034 4034 Size: 4517 SexAI A'CCwGG_T Cuts at: 919 919 Size: 4517 SfcI C'TryA_G Cuts at: 33 282 849 1560 2245 3238 3429 33 Size: 249 567 711 685 993 191 1121 Fragments arranged by size: 1121 993 711 685 567 249 191 SfoI GGC'GCC Cuts at: 147 1949 2197 147 Size: 1802 248 2467 Fragments arranged by size: 2467 1802 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4517 SmaI CCC'GGG Cuts at: 1539 1539 Size: 4517 SmlI C'TyrA_G Cuts at: 1009 1672 2587 3079 3341 1009 Size: 663 915 492 262 2185 Fragments arranged by size: 2185 915 663 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4517 SphI G_CATG'C Cuts at: 1570 2070 1570 Size: 500 4017 SspI AAT'ATT Cuts at: 1260 1506 1260 Size: 246 4271 StyI C'CwwG_G Cuts at: 107 1651 2005 107 Size: 1544 354 2619 Fragments arranged by size: 2619 1544 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2100 2233 2692 2901 2100 Size: 133 459 209 3716 Fragments arranged by size: 3716 459 209 133 TatI w'GTAC_w Cuts at: 4032 4032 Size: 4517 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4034 4066 4034 Size: 32 4485 Tth111I GACn'n_nGTC Cuts at: 2314 2314 Size: 4517 XbaI T'CTAG_A Cuts at: 1548 1548 Size: 4517 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2012 2012 Size: 4517 XmaI C'CCGG_G Cuts at: 1537 1537 Size: 4517 XmnI GAAnn'nnTTC Cuts at: 1148 1260 1455 1148 Size: 112 195 4210 Fragments arranged by size: 4210 195 112 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BpmI BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI SanDI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI