Restriction map for
pF1KB5752
(Linear) (Six-base) MAPSORT of: KIBB5752.seq Check: 4284 from: 1 to: 2241
KIBB5752.orf
With 182 enzymes: SgfI *
June 12, 2017 09:50 ..
AatII G_ACGT'C
Cuts at: 0 1994 2241
Size: 1994 247
AccI GT'mk_AC
Cuts at: 0 854 2241
Size: 854 1387
Acc65I G'GTAC_C
Cuts at: 0 422 2241
Size: 422 1819
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 801 1631 1826 2003 2241
Size: 10 791 830 195 177 238
Fragments arranged by size:
830 791 238 195 177 10
AhdI GACnn_n'nnGTC
Cuts at: 0 819 2241
Size: 819 1422
AlwNI CAG_nnn'CTG
Cuts at: 0 197 1849 1926 1981 2241
Size: 197 1652 77 55 260
Fragments arranged by size:
1652 260 197 77 55
ApaI G_GGCC'C
Cuts at: 0 328 2241
Size: 328 1913
ApoI r'AATT_y
Cuts at: 0 982 1248 2089 2241
Size: 982 266 841 152
Fragments arranged by size:
982 841 266 152
AseI AT'TA_AT
Cuts at: 0 674 719 1387 1391 1457 2180 2241
Size: 674 45 668 4 66 723 61
Fragments arranged by size:
723 674 668 66 61 45 4
AvaI C'yCGr_G
Cuts at: 0 130 141 201 332 2241
Size: 130 11 60 131 1909
Fragments arranged by size:
1909 131 130 60 11
AvrII C'CTAG_G
Cuts at: 0 1186 2241
Size: 1186 1055
BanI G'GyrC_C
Cuts at: 0 177 422 1190 2241
Size: 177 245 768 1051
Fragments arranged by size:
1051 768 245 177
BanII G_rGCy'C
Cuts at: 0 142 152 328 2241
Size: 142 10 176 1913
Fragments arranged by size:
1913 176 142 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 374 1313 1613 2241
Size: 374 939 300 628
Fragments arranged by size:
939 628 374 300
BbvCI CC'TCA_GC
Cuts at: 0 1964 2241
Size: 1964 277
BclI T'GATC_A
Cuts at: 0 2166 2241
Size: 2166 75
BglI GCCn_nnn'nGGC
Cuts at: 0 323 2241
Size: 323 1918
BglII A'GATC_T
Cuts at: 0 864 2241
Size: 864 1377
Bme1580I G_kGCm'C
Cuts at: 0 328 1195 2241
Size: 328 867 1046
Fragments arranged by size:
1046 867 328
BmrI ACTGGGnnnn_n'
Cuts at: 0 1829 2241
Size: 1829 412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 800 2156 2241
Size: 800 1356 85
Fragments arranged by size:
1356 800 85
Bpu10I CC'TnA_GC
Cuts at: 0 1964 2241
Size: 1964 277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 1596 2241
Size: 863 733 645
Fragments arranged by size:
863 733 645
BsaBI GATnn'nnATC
Cuts at: 0 1916 2241
Size: 1916 325
BsaHI Gr'CG_yC
Cuts at: 0 1991 2241
Size: 1991 250
BsaWI w'CCGG_w
Cuts at: 0 1791 2241
Size: 1791 450
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 58 88 105 135 615 645 773 803
Size: 58 30 17 30 480 30 128 30
Cuts at: 803 2241
Size: 1438
Fragments arranged by size:
1438 480 128 58 30 30 30 30
17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 403 406 505 1166 1294 2153 2241
Size: 403 3 99 661 128 859 88
Fragments arranged by size:
859 661 403 128 99 88 3
BseYI C'CCAG_C
Cuts at: 0 292 784 1194 2241
Size: 292 492 410 1047
Fragments arranged by size:
1047 492 410 292
Bsp1286I G_dGCh'C
Cuts at: 0 142 152 328 1195 2241
Size: 142 10 176 867 1046
Fragments arranged by size:
1046 867 176 142 10
BspEI T'CCGG_A
Cuts at: 0 1791 2241
Size: 1791 450
BspHI T'CATG_A
Cuts at: 0 1217 2241
Size: 1217 1024
BsrBI CCG'CTC
Cuts at: 0 96 135 2241
Size: 96 39 2106
Fragments arranged by size:
2106 96 39
BsrFI r'CCGG_y
Cuts at: 0 180 2241
Size: 180 2061
BstYI r'GATC_y
Cuts at: 0 588 864 873 912 2241
Size: 588 276 9 39 1329
Fragments arranged by size:
1329 588 276 39 9
BtgI C'CryG_G
Cuts at: 0 109 538 1956 2063 2241
Size: 109 429 1418 107 178
Fragments arranged by size:
1418 429 178 109 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 898 2241
Size: 898 1343
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 623 658 2241
Size: 623 35 1583
Fragments arranged by size:
1583 623 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1447 2241
Size: 1447 794
EaeI y'GGCC_r
Cuts at: 0 188 347 2241
Size: 188 159 1894
Fragments arranged by size:
1894 188 159
EarI CTCTTCn'nnn_
Cuts at: 0 392 1598 2241
Size: 392 1206 643
Fragments arranged by size:
1206 643 392
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 250 2241
Size: 19 231 1991
Fragments arranged by size:
1991 231 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 800 801 1631 1826 2003 2156 2241
Size: 10 790 1 830 195 177 153 85
Fragments arranged by size:
830 790 195 177 153 85 10 1
EcoO109I rG'GnC_Cy
Cuts at: 0 324 2241
Size: 324 1917
EcoRI G'AATT_C
Cuts at: 0 2089 2241
Size: 2089 152
HaeII r_GCGC'y
Cuts at: 0 223 1964 2241
Size: 223 1741 277
Fragments arranged by size:
1741 277 223
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 105 137 487 519 805 837
Size: 58 32 15 32 350 32 286 32
Cuts at: 837 1773 1805 1981 2013 2241
Size: 936 32 176 32 228
Fragments arranged by size:
936 350 286 228 176 58 32 32
32 32 32 32 15
HincII GTy'rAC
Cuts at: 0 1134 1299 2241
Size: 1134 165 942
Fragments arranged by size:
1134 942 165
HindIII A'AGCT_T
Cuts at: 0 1516 2241
Size: 1516 725
KpnI G_GTAC'C
Cuts at: 0 426 2241
Size: 426 1815
MfeI C'AATT_G
Cuts at: 0 725 2241
Size: 725 1516
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 2241
Size: 623 1618
MslI CAynn'nnrTG
Cuts at: 0 476 1421 2241
Size: 476 945 820
Fragments arranged by size:
945 820 476
MspA1I CmG'CkG
Cuts at: 0 111 345 1198 2241
Size: 111 234 853 1043
Fragments arranged by size:
1043 853 234 111
NaeI GCC'GGC
Cuts at: 0 182 2241
Size: 182 2059
NcoI C'CATG_G
Cuts at: 0 1956 2063 2241
Size: 1956 107 178
Fragments arranged by size:
1956 178 107
NgoMIV G'CCGG_C
Cuts at: 0 180 2241
Size: 180 2061
NspI r_CATG'y
Cuts at: 0 511 2039 2241
Size: 511 1528 202
Fragments arranged by size:
1528 511 202
PacI TTA_AT'TAA
Cuts at: 0 1391 2241
Size: 1391 850
PfoI T'CCnGG_A
Cuts at: 0 951 2241
Size: 951 1290
PspOMI G'GGCC_C
Cuts at: 0 324 2241
Size: 324 1917
PspXI vC'TCGA_Gb
Cuts at: 0 130 2241
Size: 130 2111
PstI C_TGCA'G
Cuts at: 0 323 2241
Size: 323 1918
PvuII CAG'CTG
Cuts at: 0 1198 2241
Size: 1198 1043
SacII CC_GC'GG
Cuts at: 0 112 2241
Size: 112 2129
SbfI CC_TGCA'GG
Cuts at: 0 323 2241
Size: 323 1918
ScaI AGT'ACT
Cuts at: 0 1818 2241
Size: 1818 423
SfcI C'TryA_G
Cuts at: 0 319 851 2241
Size: 319 532 1390
Fragments arranged by size:
1390 532 319
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 323 2241
Size: 323 1918
SmaI CCC'GGG
Cuts at: 0 143 203 2241
Size: 143 60 2038
Fragments arranged by size:
2038 143 60
SmlI C'TyrA_G
Cuts at: 0 130 878 1611 2241
Size: 130 748 733 630
Fragments arranged by size:
748 733 630 130
SrfI GCCC'GGGC
Cuts at: 0 143 2241
Size: 143 2098
SspI AAT'ATT
Cuts at: 0 901 2241
Size: 901 1340
StyI C'CwwG_G
Cuts at: 0 532 1186 1956 2063 2241
Size: 532 654 770 107 178
Fragments arranged by size:
770 654 532 178 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1599 2241
Size: 1599 642
TatI w'GTAC_w
Cuts at: 0 1743 1816 2241
Size: 1743 73 425
Fragments arranged by size:
1743 425 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 773 805 2241
Size: 773 32 1436
Fragments arranged by size:
1436 773 32
XhoI C'TCGA_G
Cuts at: 0 130 2241
Size: 130 2111
XmaI C'CCGG_G
Cuts at: 0 141 201 2241
Size: 141 60 2040
Fragments arranged by size:
2040 141 60
XmnI GAAnn'nnTTC
Cuts at: 0 1262 2241
Size: 1262 979
ZraI GAC'GTC
Cuts at: 0 1992 2241
Size: 1992 249
Enzymes that do cut:
AatII AccI Acc65I AcuI AhdI AlwNI ApaI ApoI
AseI AvaI AvrII BanI BanII BbsI BbvCI BclI
BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI Bsp1286I BspEI BspHI
BsrBI BsrFI BstYI BtgI BtgZI CspCI DrdI EaeI
EarI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII
HindIII KpnI MfeI MmeI MslI MspA1I NaeI NcoI
NgoMIV NspI PacI PfoI PspOMI PspXI PstI PvuII
SacII SbfI ScaI SfcI SfiI SmaI SmlI SrfI
SspI StyI TaqII TatI TstI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AflIII AgeI AleI AlfI
AloI ApaLI AscI AsiSI BaeI BamHI BbeI BcgI
BciVI BfrBI BlpI BmgBI BmtI BplI BsaI BsaAI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspMI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtsI ClaI DraI DraIII EagI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HpaI KasI MluI
MscI NarI NdeI NheI NotI NruI NsiI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PvuI RsrII SacI SalI SanDI SapI SexAI
SfoI SgfI SgrAI SnaBI SpeI SphI StuI SwaI
Tth111I XbaI XcmI