Restriction map for pF1KB8468
 (Circular) (Six-base) MAPSORT of: pF1KB8468.seq  Check: 6397  from: 1  to: 4004



pF1KB8468  4004 bp

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:27  ..

AccI GT'mk_AC

Cuts at:    171    1042     171 
   Size:        871    3133
 
Acc65I G'GTAC_C

Cuts at:    145    1020     145 
   Size:        875    3129
 
AclI AA'CG_TT

Cuts at:   3844    3844 
   Size:       4004
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    289     388     732    1829    2261    3008    3276     289 
   Size:         99     344    1097     432     747     268    1017

  Fragments arranged by size: 

                1097    1017     747     432     344     268      99
 
AflIII A'CryG_T

Cuts at:   1356    2460    3437    1356 
   Size:       1104     977    1923

  Fragments arranged by size: 

                1923    1104     977
 
AgeI A'CCGG_T

Cuts at:   2392    2392 
   Size:       4004
 
AlwNI CAG_nnn'CTG

Cuts at:   1309    2876    1309 
   Size:       1567    2437
 
ApaLI G'TGCA_C

Cuts at:   2774    2774 
   Size:       4004
 
ApoI r'AATT_y

Cuts at:    260     932    1008    3357    3613     260 
   Size:        672      76    2349     256     651

  Fragments arranged by size: 

                2349     672     651     256      76
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4004
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4004
 
AvaI C'yCGr_G

Cuts at:   1024    1024 
   Size:       4004
 
BamHI G'GATC_C

Cuts at:   1029    1029 
   Size:       4004
 
BanI G'GyrC_C

Cuts at:    145     679    1020    1434    1682    1717     145 
   Size:        534     341     414     248      35    2432

  Fragments arranged by size: 

                2432     534     414     341     248      35
 
BanII G_rGCy'C

Cuts at:   1018    2048    1018 
   Size:       1030    2974
 
BbeI G_GCGC'C

Cuts at:   1438    1686    1438 
   Size:        248    3756
 
BbsI GAAGACnn'nnnn_

Cuts at:    295     295 
   Size:       4004
 
BciVI GTATCCnnnnn_n'

Cuts at:   1902    2663    4004    1902 
   Size:        761    1341    1902

  Fragments arranged by size: 

                1902    1341     761
 
BclI T'GATC_A

Cuts at:    738    1524     738 
   Size:        786    3218
 
BglII A'GATC_T

Cuts at:    616    1519    3556     616 
   Size:        903    2037    1064

  Fragments arranged by size: 

                2037    1064     903
 
BlpI GC'TnA_GC

Cuts at:   1116    1116 
   Size:       4004
 
Bme1580I G_kGCm'C

Cuts at:    772    1629    1722    2778     772 
   Size:        857      93    1056    1998

  Fragments arranged by size: 

                1998    1056     857      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1216    1297    1632    3776    1216 
   Size:         81     335    2144    1444

  Fragments arranged by size: 

                2144    1444     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2371    2371 
   Size:       4004
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    605     641     605 
   Size:         36    3968
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1180    2059    2551    2849    1180 
   Size:        879     492     298    2335

  Fragments arranged by size: 

                2335     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3708    3708 
   Size:       4004
 
BsaAI yAC'GTr

Cuts at:   1357    1357 
   Size:       4004
 
BsaBI GATnn'nnATC

Cuts at:      6    1518    3555       6 
   Size:       1512    2037     455

  Fragments arranged by size: 

                2037    1512     455
 
BsaHI Gr'CG_yC

Cuts at:   1435    1683    2385    3881    1435 
   Size:        248     702    1496    1558

  Fragments arranged by size: 

                1558    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1193    1714    2392    2666    2813    3224    1193 
   Size:        521     678     274     147     411    1973

  Fragments arranged by size: 

                1973     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    633    1429    2187    2764    3260     633 
   Size:        796     758     577     496    1377

  Fragments arranged by size: 

                1377     796     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1546    1592    2800     104 
   Size:       1442      46    1208    1308

  Fragments arranged by size: 

                1442    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1018    1796    1986    2778    1018 
   Size:        778     190     792    2244

  Fragments arranged by size: 

                2244     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    772    1018    1629    1722    1796    1986    2048    2778     772 
   Size:        246     611      93      74     190      62     730    1998

  Fragments arranged by size: 

                1998     730     611     246     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3224    3224 
   Size:       4004
 
BspHI T'CATG_A

Cuts at:   3180    3180 
   Size:       4004
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1054    1268    1570    1951    1054 
   Size:        214     302     381    3107

  Fragments arranged by size: 

                3107     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2296    2350    3834    3999      50 
   Size:       2246      54    1484     165      55

  Fragments arranged by size: 

                2246    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1916    1916 
   Size:       4004
 
BsrFI r'CCGG_y

Cuts at:   2002    2183    2392    2002 
   Size:        181     209    3614

  Fragments arranged by size: 

                3614     209     181
 
BssSI C'ACGA_G

Cuts at:   2275    2633    2275 
   Size:        358    3646
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1381    1381 
   Size:       4004
 
BstBI TT'CG_AA

Cuts at:   2365    2365 
   Size:       4004
 
BstYI r'GATC_y

Cuts at:      1     363     616    1029    1496    1519    1854    2100    3112 
   Size:        362     253     413     467      23     335     246    1012

Cuts at:   3112    3198    3210    3556       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     467     449     413     362     346     335     253
                 246      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     359     720     107 
   Size:        252     361    3391

  Fragments arranged by size: 

                3391     361     252
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1954    2135    2298    1954 
   Size:        181     163    3660

  Fragments arranged by size: 

                3660     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1247    1247 
   Size:       4004
 
DraI TTT'AAA

Cuts at:   1003    1003 
   Size:       4004
 
DrdI GACnn_nn'nnGTC

Cuts at:   1710    2568    1710 
   Size:        858    3146
 
EaeI y'GGCC_r

Cuts at:    790    1589    1763    2154    2181    3526     790 
   Size:        799     174     391      27    1345    1268

  Fragments arranged by size: 

                1345    1268     799     391     174      27
 
EagI C'GGCC_G

Cuts at:   1589    1589 
   Size:       4004
 
EarI CTCTTCn'nnn_

Cuts at:    221     468     751    2027    2237     221 
   Size:        247     283    1276     210    1988

  Fragments arranged by size: 

                1988    1276     283     247     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2212    2522    2668    3814    2212 
   Size:        310     146    1146    2402

  Fragments arranged by size: 

                2402    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1016    1016 
   Size:       4004
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    289     388     605     641     732    1829    2261    3008    3276 
   Size:         99     217      36      91    1097     432     747     268

Cuts at:   3276     289 
   Size:       1017

  Fragments arranged by size: 

                1097    1017     747     432     268     217      99      91
                  36
 
EcoO109I rG'GnC_Cy

Cuts at:   1143    1143 
   Size:       4004
 
EcoRI G'AATT_C

Cuts at:   1008    1008 
   Size:       4004
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    393    2023     393 
   Size:       1630    2374
 
FspI TGC'GCA

Cuts at:   1785    1785 
   Size:       4004
 
HaeII r_GCGC'y

Cuts at:   1438    1686    2708    3687    1438 
   Size:        248    1022     979    1755

  Fragments arranged by size: 

                1755    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    883     915    1846    1876    1878    1908     883 
   Size:         32     931      30       2      30    2979

  Fragments arranged by size: 

                2979     931      32      30      30       2
 
HincII GTy'rAC

Cuts at:    663     911    1043     663 
   Size:        248     132    3624

  Fragments arranged by size: 

                3624     248     132
 
HpaI GTT'AAC

Cuts at:    911     911 
   Size:       4004
 
KasI G'GCGC_C

Cuts at:   1434    1682    1434 
   Size:        248    3756
 
KpnI G_GTAC'C

Cuts at:    149    1024     149 
   Size:        875    3129
 
MluI A'CGCG_T

Cuts at:   3437    3437 
   Size:       4004
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2675    2859    2675 
   Size:        184    3820
 
MscI TGG'CCA

Cuts at:    792    1765     792 
   Size:        973    3031
 
MslI CAynn'nnrTG

Cuts at:   1993    2120    1993 
   Size:        127    3877
 
MspA1I CmG'CkG

Cuts at:   1115    1429    1789    2802    3047    1115 
   Size:        314     360    1013     245    2072

  Fragments arranged by size: 

                2072    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2185    2185 
   Size:       4004
 
NarI GG'CG_CC

Cuts at:   1435    1683    1435 
   Size:        248    3756
 
NcoI C'CATG_G

Cuts at:    107     359     720     107 
   Size:        252     361    3391

  Fragments arranged by size: 

                3391     361     252
 
NgoMIV G'CCGG_C

Cuts at:   2183    2183 
   Size:       4004
 
NspI r_CATG'y

Cuts at:   1057    1557    2464    1057 
   Size:        500     907    2597

  Fragments arranged by size: 

                2597     907     500
 
PciI A'CATG_T

Cuts at:   2460    2460 
   Size:       4004
 
PmeI GTTT'AAAC

Cuts at:   1003    1003 
   Size:       4004
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3143    3175    3143 
   Size:         32    3972
 
PstI C_TGCA'G

Cuts at:   1051    1736    1051 
   Size:        685    3319
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4004
 
PvuII CAG'CTG

Cuts at:   1429    1789    1429 
   Size:        360    3644
 
RsrII CG'GwC_CG

Cuts at:   2199    2199 
   Size:       4004
 
SacI G_AGCT'C

Cuts at:   1018    1018 
   Size:       4004
 
SalI G'TCGA_C

Cuts at:   1041    1041 
   Size:       4004
 
SapI GCTCTTCn'nnn_

Cuts at:   2027    2237    2027 
   Size:        210    3794
 
SbfI CC_TGCA'GG

Cuts at:   1051    1051 
   Size:       4004
 
ScaI AGT'ACT

Cuts at:    246    3521     246 
   Size:       3275     729
 
SfcI C'TryA_G

Cuts at:     33     182     445    1047    1732    2725    2916      33 
   Size:        149     263     602     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     602     263     191     149
 
SfoI GGC'GCC

Cuts at:   1436    1684    1436 
   Size:        248    3756
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4004
 
SmaI CCC'GGG

Cuts at:   1026    1026 
   Size:       4004
 
SmlI C'TyrA_G

Cuts at:   1159    2074    2566    2828    1159 
   Size:        915     492     262    2335

  Fragments arranged by size: 

                2335     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4004
 
SphI G_CATG'C

Cuts at:   1057    1557    1057 
   Size:        500    3504
 
StyI C'CwwG_G

Cuts at:    107     359     720    1138    1492     107 
   Size:        252     361     418     354    2619

  Fragments arranged by size: 

                2619     418     361     354     252
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1587    1720    2179    2388    1587 
   Size:        133     459     209    3203

  Fragments arranged by size: 

                3203     459     209     133
 
TatI w'GTAC_w

Cuts at:    244     401    3519     244 
   Size:        157    3118     729

  Fragments arranged by size: 

                3118     729     157
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3521    3553    3521 
   Size:         32    3972
 
Tth111I GACn'n_nGTC

Cuts at:   1801    1801 
   Size:       4004
 
XbaI T'CTAG_A

Cuts at:    469    1035     469 
   Size:        566    3438
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    665    1499     665 
   Size:        834    3170
 
XmaI C'CCGG_G

Cuts at:   1024    1024 
   Size:       4004
 
XmnI GAAnn'nnTTC

Cuts at:    236     236 
   Size:       4004
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     HpaI
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI     PmeI     PpiI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com