(Linear) (Six-base) MAPSORT of: KIBB9962.seq Check: 1245 from: 1 to: 2076 KIBB9962.orf With 182 enzymes: SgfI * September 25, 2012 15:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1442 2076 Size: 1442 634 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 393 1144 2076 Size: 393 751 932 Fragments arranged by size: 932 751 393 AflIII A'CryG_T Cuts at: 0 698 2076 Size: 698 1378 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 484 516 662 694 2076 Size: 484 32 146 32 1382 Fragments arranged by size: 1382 484 146 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 1975 2076 Size: 1975 101 ApaI G_GGCC'C Cuts at: 0 78 2076 Size: 78 1998 ApoI r'AATT_y Cuts at: 0 524 836 1605 2076 Size: 524 312 769 471 Fragments arranged by size: 769 524 471 312 AvaI C'yCGr_G Cuts at: 0 1891 2076 Size: 1891 185 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1244 1277 2076 Size: 1244 33 799 Fragments arranged by size: 1244 799 33 BanII G_rGCy'C Cuts at: 0 78 132 217 592 1330 1423 1942 2076 Size: 78 54 85 375 738 93 519 134 Fragments arranged by size: 738 519 375 134 93 85 78 54 BbsI GAAGACnn'nnnn_ Cuts at: 0 1073 2076 Size: 1073 1003 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1312 1346 2076 Size: 1312 34 730 Fragments arranged by size: 1312 730 34 BciVI GTATCCnnnnn_n' Cuts at: 0 41 486 1791 2076 Size: 41 445 1305 285 Fragments arranged by size: 1305 445 285 41 BfrBI ATG'CAT Cuts at: 0 763 2076 Size: 763 1313 BglII A'GATC_T Cuts at: 0 1413 2076 Size: 1413 663 Bme1580I G_kGCm'C Cuts at: 0 78 2076 Size: 78 1998 BmrI ACTGGGnnnn_n' Cuts at: 0 1123 1455 1608 2076 Size: 1123 332 153 468 Fragments arranged by size: 1123 468 332 153 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 941 1874 1952 2076 Size: 941 933 78 124 Fragments arranged by size: 941 933 124 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1005 2076 Size: 1005 1071 BsaBI GATnn'nnATC Cuts at: 0 1169 2076 Size: 1169 907 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 190 220 662 692 1087 1117 1582 1612 Size: 190 30 442 30 395 30 465 30 Cuts at: 1612 2076 Size: 464 Fragments arranged by size: 465 464 442 395 190 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 553 948 1171 1587 2076 Size: 553 395 223 416 489 Fragments arranged by size: 553 489 416 395 223 BseYI C'CCAG_C Cuts at: 0 1395 1649 2076 Size: 1395 254 427 Fragments arranged by size: 1395 427 254 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 177 433 2076 Size: 177 256 1643 Fragments arranged by size: 1643 256 177 BsiHKAI G_wGCw'C Cuts at: 0 1330 1942 2076 Size: 1330 612 134 Fragments arranged by size: 1330 612 134 Bsp1286I G_dGCh'C Cuts at: 0 78 132 217 592 1330 1423 1942 2076 Size: 78 54 85 375 738 93 519 134 Fragments arranged by size: 738 519 375 134 93 85 78 54 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1442 2076 Size: 1442 634 BsrFI r'CCGG_y Cuts at: 0 767 1076 2076 Size: 767 309 1000 Fragments arranged by size: 1000 767 309 BsrGI T'GTAC_A Cuts at: 0 395 2076 Size: 395 1681 BstYI r'GATC_y Cuts at: 0 719 755 1413 1543 2076 Size: 719 36 658 130 533 Fragments arranged by size: 719 658 533 130 36 Bsu36I CC'TnA_GG Cuts at: 0 70 1238 2076 Size: 70 1168 838 Fragments arranged by size: 1168 838 70 BtsI GCAGTG_nn' Cuts at: 0 96 1402 1881 2076 Size: 96 1306 479 195 Fragments arranged by size: 1306 479 195 96 DraI TTT'AAA Cuts at: 0 1479 2076 Size: 1479 597 DraIII CAC_nnn'GTG Cuts at: 0 875 2076 Size: 875 1201 EaeI y'GGCC_r Cuts at: 0 23 2076 Size: 23 2053 EarI CTCTTCn'nnn_ Cuts at: 0 305 1120 1947 2076 Size: 305 815 827 129 Fragments arranged by size: 827 815 305 129 EcoICRI GAG'CTC Cuts at: 0 1328 1940 2076 Size: 1328 612 136 Fragments arranged by size: 1328 612 136 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 393 941 1144 1874 1952 2076 Size: 393 548 203 730 78 124 Fragments arranged by size: 730 548 393 203 124 78 EcoNI CCTnn'n_nnAGG Cuts at: 0 1438 2076 Size: 1438 638 EcoO109I rG'GnC_Cy Cuts at: 0 74 2076 Size: 74 2002 EcoRI G'AATT_C Cuts at: 0 836 1605 2076 Size: 836 769 471 Fragments arranged by size: 836 769 471 EcoRV GAT'ATC Cuts at: 0 278 2076 Size: 278 1798 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 280 312 711 743 1405 1437 1567 1599 Size: 280 32 399 32 662 32 130 32 Cuts at: 1599 1645 1677 1727 1759 1789 1821 2076 Size: 46 32 50 32 30 32 255 Fragments arranged by size: 662 399 280 255 130 50 46 32 32 32 32 32 32 32 30 HincII GTy'rAC Cuts at: 0 209 1619 2076 Size: 209 1410 457 Fragments arranged by size: 1410 457 209 HindIII A'AGCT_T Cuts at: 0 1558 2076 Size: 1558 518 HpaI GTT'AAC Cuts at: 0 209 2076 Size: 209 1867 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 58 131 1388 2076 Size: 58 73 1257 688 Fragments arranged by size: 1257 688 73 58 MslI CAynn'nnrTG Cuts at: 0 1039 2076 Size: 1039 1037 MspA1I CmG'CkG Cuts at: 0 337 2076 Size: 337 1739 NaeI GCC'GGC Cuts at: 0 769 2076 Size: 769 1307 NgoMIV G'CCGG_C Cuts at: 0 767 2076 Size: 767 1309 NsiI A_TGCA'T Cuts at: 0 765 2076 Size: 765 1311 NspI r_CATG'y Cuts at: 0 702 714 767 2076 Size: 702 12 53 1309 Fragments arranged by size: 1309 702 53 12 PciI A'CATG_T Cuts at: 0 698 2076 Size: 698 1378 PflMI CCAn_nnn'nTGG Cuts at: 0 875 2076 Size: 875 1201 PfoI T'CCnGG_A Cuts at: 0 348 2076 Size: 348 1728 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 662 694 2076 Size: 662 32 1382 Fragments arranged by size: 1382 662 32 PspOMI G'GGCC_C Cuts at: 0 74 2076 Size: 74 2002 PstI C_TGCA'G Cuts at: 0 1260 1309 2076 Size: 1260 49 767 Fragments arranged by size: 1260 767 49 PvuII CAG'CTG Cuts at: 0 337 2076 Size: 337 1739 SacI G_AGCT'C Cuts at: 0 1330 1942 2076 Size: 1330 612 134 Fragments arranged by size: 1330 612 134 SapI GCTCTTCn'nnn_ Cuts at: 0 1947 2076 Size: 1947 129 ScaI AGT'ACT Cuts at: 0 1851 2076 Size: 1851 225 SfcI C'TryA_G Cuts at: 0 963 1256 1305 1731 2076 Size: 963 293 49 426 345 Fragments arranged by size: 963 426 345 293 49 SmlI C'TyrA_G Cuts at: 0 984 2076 Size: 984 1092 SphI G_CATG'C Cuts at: 0 767 2076 Size: 767 1309 SspI AAT'ATT Cuts at: 0 640 790 1755 2005 2076 Size: 640 150 965 250 71 Fragments arranged by size: 965 640 250 150 71 StyI C'CwwG_G Cuts at: 0 776 1715 2076 Size: 776 939 361 Fragments arranged by size: 939 776 361 TatI w'GTAC_w Cuts at: 0 395 1425 1849 2076 Size: 395 1030 424 227 Fragments arranged by size: 1030 424 395 227 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1582 1614 2076 Size: 1582 32 462 Fragments arranged by size: 1582 462 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 872 2076 Size: 872 1204 XmnI GAAnn'nnTTC Cuts at: 0 791 822 1229 2076 Size: 791 31 407 847 Fragments arranged by size: 847 791 407 31 Enzymes that do cut: AarI AcuI AflIII AloI AlwNI ApaI ApoI AvaI BaeI BanII BbsI BcgI BciVI BfrBI BglII Bme1580I BmrI BpmI BpuEI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BsrGI BstYI Bsu36I BtsI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I HincII HindIII HpaI MmeI MslI MspA1I NaeI NgoMIV NsiI NspI PciI PflMI PfoI PpiI PspOMI PstI PvuII SacI SapI ScaI SfcI SmlI SphI SspI StyI TatI TstI XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI AvrII BamHI BanI BbeI BbvCI BclI BglI BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI DrdI EagI EciI FalI FseI FspI FspAI HaeII KasI KpnI MfeI MluI MscI NarI NcoI NdeI NheI NotI NruI PacI PasI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI