Restriction map for pF1KB9962
 (Linear) (Six-base) MAPSORT of: KIBB9962.seq  Check: 1245  from: 1  to: 2076



KIBB9962.orf

 With 182 enzymes: SgfI * 

                        September 25, 2012 15:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1442    2076 
   Size:       1442     634
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     393    1144    2076 
   Size:        393     751     932

  Fragments arranged by size: 

                 932     751     393
 
AflIII A'CryG_T

Cuts at:      0     698    2076 
   Size:        698    1378
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     484     516     662     694    2076 
   Size:        484      32     146      32    1382

  Fragments arranged by size: 

                1382     484     146      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1975    2076 
   Size:       1975     101
 
ApaI G_GGCC'C

Cuts at:      0      78    2076 
   Size:         78    1998
 
ApoI r'AATT_y

Cuts at:      0     524     836    1605    2076 
   Size:        524     312     769     471

  Fragments arranged by size: 

                 769     524     471     312
 
AvaI C'yCGr_G

Cuts at:      0    1891    2076 
   Size:       1891     185
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1244    1277    2076 
   Size:       1244      33     799

  Fragments arranged by size: 

                1244     799      33
 
BanII G_rGCy'C

Cuts at:      0      78     132     217     592    1330    1423    1942    2076 
   Size:         78      54      85     375     738      93     519     134

  Fragments arranged by size: 

                 738     519     375     134      93      85      78      54
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1073    2076 
   Size:       1073    1003
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1312    1346    2076 
   Size:       1312      34     730

  Fragments arranged by size: 

                1312     730      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      41     486    1791    2076 
   Size:         41     445    1305     285

  Fragments arranged by size: 

                1305     445     285      41
 
BfrBI ATG'CAT

Cuts at:      0     763    2076 
   Size:        763    1313
 
BglII A'GATC_T

Cuts at:      0    1413    2076 
   Size:       1413     663
 
Bme1580I G_kGCm'C

Cuts at:      0      78    2076 
   Size:         78    1998
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1123    1455    1608    2076 
   Size:       1123     332     153     468

  Fragments arranged by size: 

                1123     468     332     153
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     941    1874    1952    2076 
   Size:        941     933      78     124

  Fragments arranged by size: 

                 941     933     124      78
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1005    2076 
   Size:       1005    1071
 
BsaBI GATnn'nnATC

Cuts at:      0    1169    2076 
   Size:       1169     907
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     190     220     662     692    1087    1117    1582    1612 
   Size:        190      30     442      30     395      30     465      30

Cuts at:   1612    2076 
   Size:        464

  Fragments arranged by size: 

                 465     464     442     395     190      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     553     948    1171    1587    2076 
   Size:        553     395     223     416     489

  Fragments arranged by size: 

                 553     489     416     395     223
 
BseYI C'CCAG_C

Cuts at:      0    1395    1649    2076 
   Size:       1395     254     427

  Fragments arranged by size: 

                1395     427     254
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     177     433    2076 
   Size:        177     256    1643

  Fragments arranged by size: 

                1643     256     177
 
BsiHKAI G_wGCw'C

Cuts at:      0    1330    1942    2076 
   Size:       1330     612     134

  Fragments arranged by size: 

                1330     612     134
 
Bsp1286I G_dGCh'C

Cuts at:      0      78     132     217     592    1330    1423    1942    2076 
   Size:         78      54      85     375     738      93     519     134

  Fragments arranged by size: 

                 738     519     375     134      93      85      78      54
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1442    2076 
   Size:       1442     634
 
BsrFI r'CCGG_y

Cuts at:      0     767    1076    2076 
   Size:        767     309    1000

  Fragments arranged by size: 

                1000     767     309
 
BsrGI T'GTAC_A

Cuts at:      0     395    2076 
   Size:        395    1681
 
BstYI r'GATC_y

Cuts at:      0     719     755    1413    1543    2076 
   Size:        719      36     658     130     533

  Fragments arranged by size: 

                 719     658     533     130      36
 
Bsu36I CC'TnA_GG

Cuts at:      0      70    1238    2076 
   Size:         70    1168     838

  Fragments arranged by size: 

                1168     838      70
 
BtsI GCAGTG_nn'

Cuts at:      0      96    1402    1881    2076 
   Size:         96    1306     479     195

  Fragments arranged by size: 

                1306     479     195      96
 
DraI TTT'AAA

Cuts at:      0    1479    2076 
   Size:       1479     597
 
DraIII CAC_nnn'GTG

Cuts at:      0     875    2076 
   Size:        875    1201
 
EaeI y'GGCC_r

Cuts at:      0      23    2076 
   Size:         23    2053
 
EarI CTCTTCn'nnn_

Cuts at:      0     305    1120    1947    2076 
   Size:        305     815     827     129

  Fragments arranged by size: 

                 827     815     305     129
 
EcoICRI GAG'CTC

Cuts at:      0    1328    1940    2076 
   Size:       1328     612     136

  Fragments arranged by size: 

                1328     612     136
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     393     941    1144    1874    1952    2076 
   Size:        393     548     203     730      78     124

  Fragments arranged by size: 

                 730     548     393     203     124      78
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1438    2076 
   Size:       1438     638
 
EcoO109I rG'GnC_Cy

Cuts at:      0      74    2076 
   Size:         74    2002
 
EcoRI G'AATT_C

Cuts at:      0     836    1605    2076 
   Size:        836     769     471

  Fragments arranged by size: 

                 836     769     471
 
EcoRV GAT'ATC

Cuts at:      0     278    2076 
   Size:        278    1798
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     280     312     711     743    1405    1437    1567    1599 
   Size:        280      32     399      32     662      32     130      32

Cuts at:   1599    1645    1677    1727    1759    1789    1821    2076 
   Size:         46      32      50      32      30      32     255

  Fragments arranged by size: 

                 662     399     280     255     130      50      46      32
                  32      32      32      32      32      32      30
 
HincII GTy'rAC

Cuts at:      0     209    1619    2076 
   Size:        209    1410     457

  Fragments arranged by size: 

                1410     457     209
 
HindIII A'AGCT_T

Cuts at:      0    1558    2076 
   Size:       1558     518
 
HpaI GTT'AAC

Cuts at:      0     209    2076 
   Size:        209    1867
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     131    1388    2076 
   Size:         58      73    1257     688

  Fragments arranged by size: 

                1257     688      73      58
 
MslI CAynn'nnrTG

Cuts at:      0    1039    2076 
   Size:       1039    1037
 
MspA1I CmG'CkG

Cuts at:      0     337    2076 
   Size:        337    1739
 
NaeI GCC'GGC

Cuts at:      0     769    2076 
   Size:        769    1307
 
NgoMIV G'CCGG_C

Cuts at:      0     767    2076 
   Size:        767    1309
 
NsiI A_TGCA'T

Cuts at:      0     765    2076 
   Size:        765    1311
 
NspI r_CATG'y

Cuts at:      0     702     714     767    2076 
   Size:        702      12      53    1309

  Fragments arranged by size: 

                1309     702      53      12
 
PciI A'CATG_T

Cuts at:      0     698    2076 
   Size:        698    1378
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     875    2076 
   Size:        875    1201
 
PfoI T'CCnGG_A

Cuts at:      0     348    2076 
   Size:        348    1728
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     662     694    2076 
   Size:        662      32    1382

  Fragments arranged by size: 

                1382     662      32
 
PspOMI G'GGCC_C

Cuts at:      0      74    2076 
   Size:         74    2002
 
PstI C_TGCA'G

Cuts at:      0    1260    1309    2076 
   Size:       1260      49     767

  Fragments arranged by size: 

                1260     767      49
 
PvuII CAG'CTG

Cuts at:      0     337    2076 
   Size:        337    1739
 
SacI G_AGCT'C

Cuts at:      0    1330    1942    2076 
   Size:       1330     612     134

  Fragments arranged by size: 

                1330     612     134
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1947    2076 
   Size:       1947     129
 
ScaI AGT'ACT

Cuts at:      0    1851    2076 
   Size:       1851     225
 
SfcI C'TryA_G

Cuts at:      0     963    1256    1305    1731    2076 
   Size:        963     293      49     426     345

  Fragments arranged by size: 

                 963     426     345     293      49
 
SmlI C'TyrA_G

Cuts at:      0     984    2076 
   Size:        984    1092
 
SphI G_CATG'C

Cuts at:      0     767    2076 
   Size:        767    1309
 
SspI AAT'ATT

Cuts at:      0     640     790    1755    2005    2076 
   Size:        640     150     965     250      71

  Fragments arranged by size: 

                 965     640     250     150      71
 
StyI C'CwwG_G

Cuts at:      0     776    1715    2076 
   Size:        776     939     361

  Fragments arranged by size: 

                 939     776     361
 
TatI w'GTAC_w

Cuts at:      0     395    1425    1849    2076 
   Size:        395    1030     424     227

  Fragments arranged by size: 

                1030     424     395     227
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1582    1614    2076 
   Size:       1582      32     462

  Fragments arranged by size: 

                1582     462      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     872    2076 
   Size:        872    1204
 
XmnI GAAnn'nnTTC

Cuts at:      0     791     822    1229    2076 
   Size:        791      31     407     847

  Fragments arranged by size: 

                 847     791     407      31
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AloI    AlwNI     ApaI     ApoI     AvaI
     BaeI    BanII     BbsI     BcgI    BciVI    BfrBI    BglII Bme1580I
     BmrI     BpmI    BpuEI    BsaBI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrFI    BsrGI    BstYI   Bsu36I     BtsI
     DraI   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV    Hin4I   HincII  HindIII     HpaI     MmeI     MslI
   MspA1I     NaeI   NgoMIV     NsiI     NspI     PciI    PflMI     PfoI
     PpiI   PspOMI     PstI    PvuII     SacI     SapI     ScaI     SfcI
     SmlI     SphI     SspI     StyI     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI    ApaLI     AscI     AseI    AsiSI    AvrII    BamHI
     BanI     BbeI    BbvCI     BclI     BglI     BlpI    BmgBI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI
    CspCI     DrdI     EagI     EciI     FalI     FseI     FspI    FspAI
    HaeII     KasI     KpnI     MfeI     MluI     MscI     NarI     NcoI
     NdeI     NheI     NotI     NruI     PacI     PasI     PmeI     PmlI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI    TaqII  Tth111I
     XbaI     XhoI     XmaI     ZraI
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