Restriction map for pF1KE2335
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       AvaI        AvrII       BanII       
BbeI        BbvCI       BlnI        BsaBI       BsiEI       
BsiWI       BsmBI       BsoBI       BspLU11I    BsrBI       
BsrFI       BssHII      BssSI       BstAPI      BstBI       
BstEII      BstPI       Cfr10I      CpoI        DraII       
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO109I    EcoO65I     EcoRV       EcoT22I     
FauI        FseI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmeI        PpuMI       PshAI       Psp1406I    PstI        
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    StuI        SwaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccII         357  CGCG                 CG^CG
AccIII        998  TCCGGA               T^CCGGA
AcyI          660  GGCGTC               G[GA]^CG[CT]C
AflII         861  CTTAAG               C^TTAAG
AspHI        4118  GTGCTC               G[AT]GC[AT]^C
AspI         4006  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1037  TGCGCA               TGC^GCA
BanI         1498  GGCACC               G^G[CT][GA]CC
BbrPI        3589  CACGTG               CAC^GTG
BcgI         4091  CGAAGATCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          330  CCCGG                CC^[GC]GG
BfrI          861  CTTAAG               C^TTAAG
BglI         2389  GCCCCTCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          920  GCTCAGC              GC^T[ACGT]AGC
BmyI         4118  GTGCTC               G[AGT]GC[ACT]^C
Bpu1102I      920  GCTCAGC              GC^T[ACGT]AGC
BsaHI         660  GGCGTC               G[GA]^CG[CT]C
BseAI         998  TCCGGA               T^CCGGA
BsgI         1961  GTGCAG               GTGCAG(16/14)
BsiHKAI      4118  GTGCTC               G[AT]GC[AT]^C
BsmFI        1882  GGGAC                GGGAC(10/14)
Bsp1286I     4118  GTGCTC               G[AGT]GC[ACT]^C
Bsp1407I     1071  TGTACA               T^GTACA
BspDI        3805  ATCGAT               AT^CGAT
BspEI         998  TCCGGA               T^CCGGA
BspHI        3416  TCATGA               T^CATGA
BspMI        2905  GCAGGT               ACCTGC(4/8)
BsrGI        1071  TGTACA               T^GTACA
Bst1107I     1684  GTATAC               GTA^TAC
BstUI         357  CGCG                 CG^CG
BstXI        3801  CCAGATCGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1684  GTATAC               GTA^TAC
CelII         920  GCTCAGC              GC^T[ACGT]AGC
ClaI         3805  ATCGAT               AT^CGAT
DsaI         4041  CCATGG               C^C[GA][CT]GG
EcoNI         702  CCTTTTTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI         1037  TGCGCA               TGC^GCA
HaeII          89  AGCGCC               [GA]GCGC^[CT]
Hin1I         660  GGCGTC               G[GA]^CG[CT]C
HpaI         2329  GTTAAC               GTT^AAC
MroI          998  TCCGGA               T^CCGGA
MspA1I         51  CAGCTG               C[AC]G^C[GT]G
MvnI          357  CGCG                 CG^CG
NciI          330  CCCGG                CC^[GC]GG
NcoI         4041  CCATGG               C^CATGG
NruI          356  TCGCGA               TCG^CGA
PflFI        4006  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        3711  CCAGAGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        3589  CACGTG               CAC^GTG
PmlI         3589  CACGTG               CAC^GTG
PvuII          51  CAGCTG               CAG^CTG
RcaI         3416  TCATGA               T^CATGA
SphI         1567  GCATGC               GCATG^C
SspBI        1071  TGTACA               T^GTACA
Tth111I      4006  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       3711  CCAGAGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          513  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI         1684  GTATAC               GT^[AC][GT]AC
             2801  GTCTAC              
AflIII       2894  ACACGT               A^C[GA][CT]GT
             3588  ACACGT              
AocI         2392  CCTCAGG              CC^T[ACGT]AGG
             3614  CCTCAGG             
AvaII          56  GGACC                G^G[AT]CC
             2562  GGTCC               
BbsI          486  GAAGAC               GAAGAC(2/6)
             2479  GAAGAC              
BciVI          79  GGATAC               GTATCC(6/5)
             1726  GGATAC              
BclI         2261  TGATCA               T^GATCA
             3933  TGATCA              
BpuAI         486  GAAGAC               GAAGAC(2/6)
             2479  GAAGAC              
BsaAI        2755  TACGTG               [CT]AC^GT[GA]
             3589  CACGTG              
BsaI         1990  GAGACC               GGTCTC(1/5)
             3967  GGTCTC              
BsaWI         998  TCCGGA               [AT]^CCGG[AT]
             4355  ACCGGA              
BsmI          446  GAATGC               GAATGC(1/-1)
             4225  GAATGC              
Bsu36I       2392  CCTCAGG              CC^T[ACGT]AGG
             3614  CCTCAGG             
DraIII       1640  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3191  CACTTCGTG           
Eco81I       2392  CCTCAGG              CC^T[ACGT]AGG
             3614  CCTCAGG             
EcoRI        2094  GAATTC               G^AATTC
             3582  GAATTC              
FbaI         2261  TGATCA               T^GATCA
             3933  TGATCA              
HgaI          661  GCGTC                GACGC(5/10)
             1556  GCGTC               
HindIII      3171  AAGCTT               A^AGCTT
             4000  AAGCTT              
NspI         1567  GCATGC               [GA]CATG^[CT]
             3503  ACATGC              
ScaI         2683  AGTACT               AGT^ACT
             3751  AGTACT              
XcmI         3446  CCAGCATAGATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4115  CCAGTGCTCATTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AseI        AsnI        Asp700I     
BalI        BamHI       BbvI        BfaI        BglII       
BpmI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        EaeI        EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        PshBI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       XmnI        
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