Restriction map for pF1KE2359
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        ApaI        ApaLI       ApoI        
AscI        Asp718I     AspEI       AspHI       AspI        
AvaII       AvrII       BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        Bsp1407I    
BspEI       BspHI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CelII       Cfr10I      CpoI        
DraI        DraII       DraIII      DsaI        EagI        
Eam1105I    EarI        EclXI       Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
HgaI        HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MfeI        MluI        
MroI        MspA1I      MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SpeI        Sse8387I    SspBI       StyI        
SwaI        Tth111I     XbaI        XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII         298  GACGTC               GACGT^C
AcyI          298  GACGTC               G[GA]^CG[CT]C
AflIII       1065  ACATGT               A^C[GA][CT]GT
Aor51HI       995  AGCGCT               AGC^GCT
Asp700I      1004  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1111  CTCGAG               C^[CT]CG[GA]G
AviII         407  TGCGCA               TGC^GCA
BalI           95  TGGCCA               TGG^CCA
BcnI          914  CCGGG                CC^[GC]GG
BmyI          815  GGGCAC               G[AGT]GC[ACT]^C
BsaAI         543  TACGTA               [CT]AC^GT[GA]
BsaBI          93  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         298  GACGTC               G[GA]^CG[CT]C
BsaI          760  GAGACC               GGTCTC(1/5)
BsgI         1087  CTGCAC               GTGCAG(16/14)
BsmAI         760  GAGAC                GTCTC(1/5)
BsoBI        1111  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      815  GGGCAC               G[AGT]GC[ACT]^C
BspDI         318  ATCGAT               AT^CGAT
BspLU11I     1065  ACATGT               A^CATGT
Cfr13I       1020  GGGCC                G^G[ACGT]CC
ClaI          318  ATCGAT               AT^CGAT
DrdI          856  GACTACACTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI           95  TGGCCA               [CT]^GGCC[GA]
Eco47III      995  AGCGCT               AGC^GCT
FspI          407  TGCGCA               TGC^GCA
HaeII         995  AGCGCT               [GA]GCGC^[CT]
HapII         914  CCGG                 C^CGG
Hin1I         298  GACGTC               G[GA]^CG[CT]C
HincII        562  GTCAAC               GT[CT]^[GA]AC
HindII        562  GTCAAC               GT[CT]^[GA]AC
HpaII         914  CCGG                 C^CGG
MamI           93  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI          95  TGGCCA               TGG^CCA
MscI           95  TGGCCA               TGG^CCA
MslI          786  CATCATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          914  CCGG                 C^CGG
NciI          914  CCGGG                CC^[GC]GG
PaeR7I       1111  CTCGAG               C^TCGAG
PflMI         372  CCAGCCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I       1020  GGGCC                G^G[ACGT]CC
SfcI          384  CTATAG               C^T[GA][CT]AG
SmlI         1111  CTCGAG               C^T[CT][GA]AG
SnaBI         543  TACGTA               TAC^GTA
SphI          663  GCATGC               GCATG^C
SspI          835  AATATT               AAT^ATT
StuI          715  AGGCCT               AGG^CCT
Van91I        372  CCAGCCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1111  CTCGAG               C^TCGAG
XmnI         1004  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AciI          639  CCGC                 CCGC(-3/-1)
              193  GCGG                
AseI          417  ATTAAT               AT^TAAT
             1051  ATTAAT              
AsnI          417  ATTAAT               AT^TAAT
             1051  ATTAAT              
BamHI         843  GGATCC               G^GATCC
             1057  GGATCC              
BanI          229  GGTGCC               G^G[CT][GA]CC
              816  GGCACC              
BbsI         1004  GAAGAC               GAAGAC(2/6)
              553  GTCTTC              
BpuAI        1004  GAAGAC               GAAGAC(2/6)
              553  GTCTTC              
BstNI         868  CCTGG                CC^[AT]GG
             1146  CCTGG               
BstXI         233  CCATTGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              833  CCAATATTGTGG        
CfoI          408  GCGC                 GCG^C
              996  GCGC                
EcoRII        868  CCTGG                ^CC[AT]GG
             1146  CCTGG               
HhaI          408  GCGC                 GCG^C
              996  GCGC                
HinP1I        408  GCGC                 G^CGC
              996  GCGC                
HinfI        1141  GAATC                G^A[ACGT]TC
             1150  GAATC               
MboII        1004  GAAGA                GAAGA(8/7)
              554  TCTTC               
MseI          418  TTAA                 T^TAA
             1052  TTAA                
MvaI          868  CCTGG                CC^[AT]GG
             1146  CCTGG               
MwoI          213  GCTGGTGATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              740  GCACTCATCGC         
NspI          663  GCATGC               [GA]CATG^[CT]
             1065  ACATGT              
PshBI         417  ATTAAT               AT^TAAT
             1051  ATTAAT              
TfiI         1141  GAATC                G^A[AT]TC
             1150  GAATC               
Tru9I         418  TTAA                 T^TAA
             1052  TTAA                
Tsp45I        649  GTCAC                ^GT[GC]AC
              829  GTCAC               
TspRI          47  CAGTG                CA[GC]TG[ACGT][ACGT]^
              861  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AfaI        AluI        AlwI        BbvI        BsaJI       
BseRI       BsiYI       BslI        BsmFI       BspMI       
BssKI       BstF5I      BstYI       Cac8I       DdeI        
DpnII       Fnu4HI      FokI        HaeIII      HphI        
ItaI        MaeII       MaeIII      MboI        MflI        
MnlI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      ScrFI       SfaNI       TaqI        TseI        
Tsp509I     TthHB8I     XhoII       
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