Restriction map for pF1KE2359
 (Circular) (Six-base) MAPSORT of: pF1KE2359.seq  Check: 8525  from: 1  to: 4274



pF1KE2359  4274 bp

 With 182 enzymes: SgfI * 

                        June 23, 2011 13:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    700     972     700 
   Size:        272    4002
 
AatII G_ACGT'C

Cuts at:    410     410 
   Size:       4274
 
AccI GT'mk_AC

Cuts at:   1312    1312 
   Size:       4274
 
Acc65I G'GTAC_C

Cuts at:   1290    1290 
   Size:       4274
 
AclI AA'CG_TT

Cuts at:   4114    4114 
   Size:       4274
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2099    2531    3278    3546    2099 
   Size:        432     747     268    2827

  Fragments arranged by size: 

                2827     747     432     268
 
AfeI AGC'GCT

Cuts at:   1105    1105 
   Size:       4274
 
AflIII A'CryG_T

Cuts at:   1173    1626    2730    3707    1173 
   Size:        453    1104     977    1740

  Fragments arranged by size: 

                1740    1104     977     453
 
AgeI A'CCGG_T

Cuts at:   2662    2662 
   Size:       4274
 
AlwNI CAG_nnn'CTG

Cuts at:   1579    3146    1579 
   Size:       1567    2707
 
ApaLI G'TGCA_C

Cuts at:   3044    3044 
   Size:       4274
 
ApoI r'AATT_y

Cuts at:   1278    3627    3883    1278 
   Size:       2349     256    1669

  Fragments arranged by size: 

                2349    1669     256
 
AseI AT'TA_AT

Cuts at:     20     526    1160      20 
   Size:        506     634    3134

  Fragments arranged by size: 

                3134     634     506
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4274
 
AvaI C'yCGr_G

Cuts at:   1219    1294    1219 
   Size:         75    4199
 
BamHI G'GATC_C

Cuts at:    951    1165    1299     951 
   Size:        214     134    3926

  Fragments arranged by size: 

                3926     214     134
 
BanI G'GyrC_C

Cuts at:    337     924    1290    1704    1952    1987     337 
   Size:        587     366     414     248      35    2624

  Fragments arranged by size: 

                2624     587     414     366     248      35
 
BanII G_rGCy'C

Cuts at:   1288    2318    1288 
   Size:       1030    3244
 
BbeI G_GCGC'C

Cuts at:   1708    1956    1708 
   Size:        248    4026
 
BbsI GAAGACnn'nnnn_

Cuts at:    654    1119     654 
   Size:        465    3809
 
BciVI GTATCCnnnnn_n'

Cuts at:   2172    2933    4274    2172 
   Size:        761    1341    2172

  Fragments arranged by size: 

                2172    1341     761
 
BclI T'GATC_A

Cuts at:   1794    1794 
   Size:       4274
 
BglII A'GATC_T

Cuts at:   1789    3826    1789 
   Size:       2037    2237
 
BlpI GC'TnA_GC

Cuts at:   1386    1386 
   Size:       4274
 
Bme1580I G_kGCm'C

Cuts at:    927    1899    1992    3048     927 
   Size:        972      93    1056    2153

  Fragments arranged by size: 

                2153    1056     972      93
 
BmgBI CAC'GTC

Cuts at:   1069    1069 
   Size:       4274
 
BmrI ACTGGGnnnn_n'

Cuts at:   1486    1567    1902    4046    1486 
   Size:         81     335    2144    1714

  Fragments arranged by size: 

                2144    1714     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    842    2641     842 
   Size:       1799    2475
 
Bpu10I CC'TnA_GC

Cuts at:    256     764     256 
   Size:        508    3766
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1450    2329    2821    3119    1450 
   Size:        879     492     298    2605

  Fragments arranged by size: 

                2605     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    862    3978     862 
   Size:       3116    1158
 
BsaAI yAC'GTr

Cuts at:    653    1627     653 
   Size:        974    3300
 
BsaBI GATnn'nnATC

Cuts at:      6     205    1788    3825       6 
   Size:        199    1583    2037     455

  Fragments arranged by size: 

                2037    1583     455     199
 
BsaHI Gr'CG_yC

Cuts at:    407    1705    1953    2655    4151     407 
   Size:       1298     248     702    1496     530

  Fragments arranged by size: 

                1496    1298     702     530     248
 
BsaWI w'CCGG_w

Cuts at:   1463    1984    2662    2936    3083    3494    1463 
   Size:        521     678     274     147     411    2243

  Fragments arranged by size: 

                2243     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    154     715     801     154 
   Size:        561      86    3627

  Fragments arranged by size: 

                3627     561      86
 
BseYI C'CCAG_C

Cuts at:    242     531     542     920    1699    2457    3034    3530     242 
   Size:        289      11     378     779     758     577     496     986

  Fragments arranged by size: 

                 986     779     758     577     496     378     289      11
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1180    1180 
   Size:       4274
 
BsiEI CG_ry'CG

Cuts at:    104    1816    1862    3070     104 
   Size:       1712      46    1208    1308

  Fragments arranged by size: 

                1712    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1288    2066    2256    3048    1288 
   Size:        778     190     792    2514

  Fragments arranged by size: 

                2514     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    927    1288    1899    1992    2066    2256    2318    3048     927 
   Size:        361     611      93      74     190      62     730    2153

  Fragments arranged by size: 

                2153     730     611     361     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3494    3494 
   Size:       4274
 
BspHI T'CATG_A

Cuts at:   3450    3450 
   Size:       4274
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    227     700     972    1324    1538    1840    2221     227 
   Size:        473     272     352     214     302     381    2280

  Fragments arranged by size: 

                2280     473     381     352     302     272     214
 
BsrBI CCG'CTC

Cuts at:     50    2566    2620    4104    4269      50 
   Size:       2516      54    1484     165      55

  Fragments arranged by size: 

                2516    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2186    2186 
   Size:       4274
 
BsrFI r'CCGG_y

Cuts at:   2272    2453    2662    2272 
   Size:        181     209    3884

  Fragments arranged by size: 

                3884     209     181
 
BssSI C'ACGA_G

Cuts at:   2545    2903    2545 
   Size:        358    3916
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1651    1651 
   Size:       4274
 
BstBI TT'CG_AA

Cuts at:   2635    2635 
   Size:       4274
 
BstXI CCAn_nnnn'nTGG

Cuts at:    348     948     348 
   Size:        600    3674
 
BstYI r'GATC_y

Cuts at:      1     303     782     951    1165    1299    1766    1789    2124 
   Size:        302     479     169     214     134     467      23     335

Cuts at:   2124    2370    3382    3468    3480    3826       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     479     467     449     346     335     302     246
                 214     169     134      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4274
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    504     838    2224    2405    2568     504 
   Size:        334    1386     181     163    2210

  Fragments arranged by size: 

                2210    1386     334     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1517    1517 
   Size:       4274
 
ClaI AT'CG_AT

Cuts at:    427     427 
   Size:       4274
 
DraI TTT'AAA

Cuts at:   1273    1273 
   Size:       4274
 
DrdI GACnn_nn'nnGTC

Cuts at:    970    1980    2838     970 
   Size:       1010     858    2406

  Fragments arranged by size: 

                2406    1010     858
 
EaeI y'GGCC_r

Cuts at:    203    1859    2033    2424    2451    3796     203 
   Size:       1656     174     391      27    1345     681

  Fragments arranged by size: 

                1656    1345     681     391     174      27
 
EagI C'GGCC_G

Cuts at:   1859    1859 
   Size:       4274
 
EarI CTCTTCn'nnn_

Cuts at:   2297    2507    2297 
   Size:        210    4064
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2482    2792    2938    4084    2482 
   Size:        310     146    1146    2672

  Fragments arranged by size: 

                2672    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1286    1286 
   Size:       4274
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   2099    2531    3278    3546    2099 
   Size:        432     747     268    2827

  Fragments arranged by size: 

                2827     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:   1413    1413 
   Size:       4274
 
EcoRI G'AATT_C

Cuts at:   1278    1278 
   Size:       4274
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2293    2293 
   Size:       4274
 
FspI TGC'GCA

Cuts at:    517    2055     517 
   Size:       1538    2736
 
HaeII r_GCGC'y

Cuts at:   1107    1708    1956    2978    3957    1107 
   Size:        601     248    1022     979    1424

  Fragments arranged by size: 

                1424    1022     979     601     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    495     527    1234    1266    2116    2146    2148    2178     495 
   Size:         32     707      32     850      30       2      30    2591

  Fragments arranged by size: 

                2591     850     707      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    672    1313     672 
   Size:        641    3633
 
KasI G'GCGC_C

Cuts at:   1704    1952    1704 
   Size:        248    4026
 
KpnI G_GTAC'C

Cuts at:   1294    1294 
   Size:       4274
 
MluI A'CGCG_T

Cuts at:   3707    3707 
   Size:       4274
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2945    3129    2945 
   Size:        184    4090
 
MscI TGG'CCA

Cuts at:    205    2035     205 
   Size:       1830    2444
 
MslI CAynn'nnrTG

Cuts at:    898    2263    2390     898 
   Size:       1365     127    2782

  Fragments arranged by size: 

                2782    1365     127
 
MspA1I CmG'CkG

Cuts at:   1385    1699    2059    3072    3317    1385 
   Size:        314     360    1013     245    2342

  Fragments arranged by size: 

                2342    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2455    2455 
   Size:       4274
 
NarI GG'CG_CC

Cuts at:   1705    1953    1705 
   Size:        248    4026
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4274
 
NgoMIV G'CCGG_C

Cuts at:   2453    2453 
   Size:       4274
 
NspI r_CATG'y

Cuts at:    775    1177    1327    1827    2734     775 
   Size:        402     150     500     907    2315

  Fragments arranged by size: 

                2315     907     500     402     150
 
PasI CC'CwG_GG

Cuts at:    976     976 
   Size:       4274
 
PciI A'CATG_T

Cuts at:   1173    2730    1173 
   Size:       1557    2717
 
PflMI CCAn_nnn'nTGG

Cuts at:    486     486 
   Size:       4274
 
PmeI GTTT'AAAC

Cuts at:   1273    1273 
   Size:       4274
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    631     663    3413    3445     631 
   Size:         32    2750      32    1460

  Fragments arranged by size: 

                2750    1460      32      32
 
PstI C_TGCA'G

Cuts at:   1321    2006    1321 
   Size:        685    3589
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4274
 
PvuII CAG'CTG

Cuts at:   1699    2059    1699 
   Size:        360    3914
 
RsrII CG'GwC_CG

Cuts at:   2469    2469 
   Size:       4274
 
SacI G_AGCT'C

Cuts at:   1288    1288 
   Size:       4274
 
SalI G'TCGA_C

Cuts at:   1311    1311 
   Size:       4274
 
SapI GCTCTTCn'nnn_

Cuts at:   2297    2507    2297 
   Size:        210    4064
 
SbfI CC_TGCA'GG

Cuts at:   1321    1321 
   Size:       4274
 
ScaI AGT'ACT

Cuts at:   3791    3791 
   Size:       4274
 
SfcI C'TryA_G

Cuts at:     33     492    1317    2002    2995    3186      33 
   Size:        459     825     685     993     191    1121

  Fragments arranged by size: 

                1121     993     825     685     459     191
 
SfoI GGC'GCC

Cuts at:   1706    1954    1706 
   Size:        248    4026
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4274
 
SmaI CCC'GGG

Cuts at:   1296    1296 
   Size:       4274
 
SmlI C'TyrA_G

Cuts at:   1219    1429    2344    2836    3098    1219 
   Size:        210     915     492     262    2395

  Fragments arranged by size: 

                2395     915     492     262     210
 
SnaBI TAC'GTA

Cuts at:    653     653 
   Size:       4274
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4274
 
SphI G_CATG'C

Cuts at:    775    1327    1827     775 
   Size:        552     500    3222

  Fragments arranged by size: 

                3222     552     500
 
SspI AAT'ATT

Cuts at:    945     945 
   Size:       4274
 
StuI AGG'CCT

Cuts at:    825     825 
   Size:       4274
 
StyI C'CwwG_G

Cuts at:    107    1408    1762     107 
   Size:       1301     354    2619

  Fragments arranged by size: 

                2619    1301     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1857    1990    2449    2658    1857 
   Size:        133     459     209    3473

  Fragments arranged by size: 

                3473     459     209     133
 
TatI w'GTAC_w

Cuts at:   1176    3789    1176 
   Size:       2613    1661
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3791    3823    3791 
   Size:         32    4242
 
Tth111I GACn'n_nGTC

Cuts at:   2071    2071 
   Size:       4274
 
XbaI T'CTAG_A

Cuts at:   1305    1305 
   Size:       4274
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1769    1769 
   Size:       4274
 
XhoI C'TCGA_G

Cuts at:   1219    1219 
   Size:       4274
 
XmaI C'CCGG_G

Cuts at:   1294    1294 
   Size:       4274
 
XmnI GAAnn'nnTTC

Cuts at:   1116    1116 
   Size:       4274
 
ZraI GAC'GTC

Cuts at:    408     408 
   Size:       4274
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PasI     PciI    PflMI     PmeI     PpiI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

    AflII     AhdI     AleI     AlfI     AloI     ApaI     AscI    AvrII
     BaeI    BbvCI     BcgI    BfrBI     BglI     BmtI     BpmI    BsaXI
    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I
    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI     PfoI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI     SrfI     SwaI
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