Restriction map for pF1KB6281
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AlwNI       AocI        Aor51HI     ApaI        AscI        
Asp700I     Asp718I     AspI        AvaI        AvrII       
BalI        BamHI       BbeI        BbrPI       BbvCI       
BclI        BcnI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BseAI       BsiEI       
BsiWI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BsrBI       BsrDI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      EaeI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRV       
EcoT22I     FbaI        FseI        HpaI        KasI        
KpnI        KspI        MamI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PmaCI       PmeI        
PmlI        Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tth111I     Van91I      
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII         611  GACGTC               GACGT^C
AcyI          611  GACGTC               G[GA]^CG[CT]C
AflIII         11  ACATGT               A^C[GA][CT]GT
AgeI         1441  ACCGGT               A^CCGGT
AhdI           25  GACTCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2115  GTGCAC               G^TGCAC
ApaLI        2115  GTGCAC               G^TGCAC
AspEI          25  GACTCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII        1207  GAGCTC               G[GA]GC[CT]^C
BglII          93  AGATCT               A^GATCT
BpmI         1769  CTCCAG               CTGGAG(16/14)
BsaHI         611  GACGTC               G[GA]^CG[CT]C
BsaWI        1441  ACCGGT               [AT]^CCGG[AT]
BsgI          171  GTGCAG               GTGCAG(16/14)
BsmI          478  GAATGC               GAATGC(1/-1)
BspLU11I       11  ACATGT               A^CATGT
BsrFI        1441  ACCGGT               [GA]^CCGG[CT]
BstEII       1144  GGTGACC              G^GT[ACGT]ACC
BstPI        1144  GGTGACC              G^GT[ACGT]ACC
Cfr10I       1441  ACCGGT               [GA]^CCGG[CT]
DraII        1708  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DrdI          786  GACCCCAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1517  CCATGG               C^C[GA][CT]GG
Eam1105I       25  GACTCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I     1708  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1144  GGTGACC              G^GT[ACGT]ACC
EcoRI         558  GAATTC               G^AATTC
FauI         2130  GCGGG                CCCGC(4/6)
HaeII        2024  AGCGCC               [GA]GCGC^[CT]
HgaI         2255  GACGC                GACGC(5/10)
Hin1I         611  GACGTC               G[GA]^CG[CT]C
HincII        590  GTCAAC               GT[CT]^[GA]AC
HindII        590  GTCAAC               GT[CT]^[GA]AC
HindIII      2382  AAGCTT               A^AGCTT
NcoI         1517  CCATGG               C^CATGG
NheI          264  GCTAGC               G^CTAGC
NspI           11  ACATGT               [GA]CATG^[CT]
PinAI        1441  ACCGGT               A^CCGGT
PpuMI        1708  AGGTCCT              [GA]G^G[AT]CC[CT]
PshAI        1721  GACACAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          872  CTGCAG               CTGCA^G
SacI         1207  GAGCTC               GAGCT^C
StuI          876  AGGCCT               AGG^CCT
XbaI         1219  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AflII         430  CTTAAG               C^TTAAG
              849  CTTAAG              
AseI         1168  ATTAAT               AT^TAAT
             1192  ATTAAT              
AsnI         1168  ATTAAT               AT^TAAT
             1192  ATTAAT              
AspHI        1207  GAGCTC               G[AT]GC[AT]^C
             2115  GTGCAC              
AvaII        1709  GGTCC                G^G[AT]CC
             2082  GGACC               
AviII        2213  TGCGCA               TGC^GCA
             2387  TGCGCA              
BcgI           51  CGAGTGTGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1056  CGAGTTGTATGC        
BciVI         138  GGATAC               GTATCC(6/5)
             2203  GGATAC              
BfrI          430  CTTAAG               C^TTAAG
              849  CTTAAG              
BmyI         1207  GAGCTC               G[AGT]GC[ACT]^C
             2115  GTGCAC              
BsiHKAI      1207  GAGCTC               G[AT]GC[AT]^C
             2115  GTGCAC              
BsmBI         422  CGTCTC               CGTCTC(1/5)
              344  GAGACG              
Bsp1286I     1207  GAGCTC               G[AGT]GC[ACT]^C
             2115  GTGCAC              
BspMI        1326  GCAGGT               ACCTGC(4/8)
             2216  GCAGGT              
BssSI        1102  CACGAG               CACGAG(-5/-1)
             1924  CACGAG              
FspI         2213  TGCGCA               TGC^GCA
             2387  TGCGCA              
HapII        1045  CCGG                 C^CGG
             1442  CCGG                
HpaII        1045  CCGG                 C^CGG
             1442  CCGG                
MfeI          542  CAATTG               C^AATTG
             2015  CAATTG              
MspI         1045  CCGG                 C^CGG
             1442  CCGG                
MunI          542  CAATTG               C^AATTG
             2015  CAATTG              
PshBI        1168  ATTAAT               AT^TAAT
             1192  ATTAAT              
SapI          415  GAAGAGC              GCTCTTC(1/4)
             2020  GAAGAGC             
TaqI           50  TCGA                 T^CGA
             1719  TCGA                
TthHB8I        50  TCGA                 T^CGA
             1719  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BanI        BbsI        BbvI        BfaI        
BpuAI       BsaI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EarI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XhoII       
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