Restriction map for pF1KB5987
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BcgI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstF5I      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FokI        FseI        
FspI        HaeII       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        Tth111I     TthHB8I     
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AcyI         1385  GACGCC               G[GA]^CG[CT]C
AflIII        631  ACATGT               A^C[GA][CT]GT
AlwNI        1608  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       388  GAAGTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         1631  TGGCCA               TGG^CCA
BclI          747  TGATCA               T^GATCA
BsaAI        1414  TACGTG               [CT]AC^GT[GA]
BsaHI        1385  GACGCC               G[GA]^CG[CT]C
BsiWI         661  CGTACG               C^GTACG
BspLU11I      631  ACATGT               A^CATGT
BstXI         183  CCATTCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         972  GGATCT               [GA]^GATC[CT]
CfoI          981  GCGC                 GCG^C
DsaI         1275  CCGTGG               C^C[GA][CT]GG
EaeI         1631  TGGCCA               [CT]^GGCC[GA]
EarI         1542  GAAGAG               CTCTTC(1/4)
EcoNI        1229  CCTATGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          747  TGATCA               T^GATCA
HgaI         1385  GACGC                GACGC(5/10)
HhaI          981  GCGC                 GCG^C
Hin1I        1385  GACGCC               G[GA]^CG[CT]C
HinP1I        981  GCGC                 G^CGC
Ksp632I      1542  GAAGAG               CTCTTC(1/4)
MflI          972  GGATCT               [GA]^GATC[CT]
MluNI        1631  TGGCCA               TGG^CCA
MscI         1631  TGGCCA               TGG^CCA
PflMI         649  CCAGAAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         230  GGGACCT              [GA]G^G[AT]CC[CT]
PstI         1624  CTGCAG               CTGCA^G
SacI         1444  GAGCTC               GAGCT^C
Van91I        649  CCAGAAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         972  GGATCT               [GA]^GATC[CT]
XmnI          388  GAAGTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AvaI          262  CCCGAG               C^[CT]CG[GA]G
             1475  CTCGGG              
BanII        1169  GGGCTC               G[GA]GC[CT]^C
             1444  GAGCTC              
BcnI          228  CCGGG                CC^[GC]GG
             1167  CCGGG               
BpmI         1420  CTCCAG               CTGGAG(16/14)
             1441  CTGGAG              
BsmI         1239  GAATGC               GAATGC(1/-1)
              461  GCATTC              
BsoBI         262  CCCGAG               C^[CT]CG[GA]G
             1475  CTCGGG              
BsrDI         600  CATTGC               GCAATG(2/0)
              830  GCAATG              
DraII         230  GGGACCT              [GA]G^G[ACGT]CC[CT]
             1679  AGGGCCT             
EcoO109I      230  GGGACCT              [GA]G^G[ACGT]CC[CT]
             1679  AGGGCCT             
EcoT14I       383  CCTTGG               C^C[AT][AT]GG
             1034  CCAAGG              
MslI            5  CACTTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1635  CATGCCAGTG          
NciI          228  CCGGG                CC^[GC]GG
             1167  CCGGG               
NspI          341  GCATGT               [GA]CATG^[CT]
              631  ACATGT              
PleI          681  GACTC                GAGTC(4/5)
               24  GAGTC               
SfcI          171  CTACAG               C^T[GA][CT]AG
             1624  CTGCAG              
StyI          383  CCTTGG               C^C[AT][AT]GG
             1034  CCAAGG              
Tsp45I        886  GTCAC                ^GT[GC]AC
             1348  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BbvI        BciVI       
BfaI        BmyI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstAPI      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SmlI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       
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