Restriction map for pF1KE1779
##################################
# NO CUT ENZYMES (  172 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaI        BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoT22I     FbaI        FseI        
FspI        HaeII       Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MspA1I      MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        TaqI        TspRI       Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AciI          381  GCGG                 CCGC(-3/-1)
AspHI         586  GAGCTC               G[AT]GC[AT]^C
AvaI          253  CCCGAG               C^[CT]CG[GA]G
AvaII         269  GGACC                G^G[AT]CC
BanII         586  GAGCTC               G[GA]GC[CT]^C
BbrPI         109  CACGTG               CAC^GTG
BfaI          434  CTAG                 C^TAG
BpmI          494  CTGGAG               CTGGAG(16/14)
BsaAI         109  CACGTG               [CT]AC^GT[GA]
BsaWI         266  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       586  GAGCTC               G[AT]GC[AT]^C
BsmAI         647  GTCTC                GTCTC(1/5)
BsmFI         236  GGGAC                GGGAC(10/14)
BsoBI         253  CCCGAG               C^[CT]CG[GA]G
BspMI         725  ACCTGC               ACCTGC(4/8)
BstYI         573  AGATCC               [GA]^GATC[CT]
CfoI          318  GCGC                 GCG^C
DrdI          222  GACTATGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          169  GAAGAG               CTCTTC(1/4)
EcoRV         280  GATATC               GAT^ATC
EcoT14I       116  CCAAGG               C^C[AT][AT]GG
FauI          381  GCGGG                CCCGC(4/6)
HapII         267  CCGG                 C^CGG
HgaI          370  GACGC                GACGC(5/10)
HhaI          318  GCGC                 GCG^C
HinP1I        318  GCGC                 G^CGC
HinfI         675  GAATC                G^A[ACGT]TC
HpaII         267  CCGG                 C^CGG
Ksp632I       169  GAAGAG               CTCTTC(1/4)
MaeI          434  CTAG                 C^TAG
MflI          573  AGATCC               [GA]^GATC[CT]
MspI          267  CCGG                 C^CGG
MwoI           63  GCTCTTGGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PmaCI         109  CACGTG               CAC^GTG
PmlI          109  CACGTG               CAC^GTG
SacI          586  GAGCTC               GAGCT^C
SapI          169  GAAGAGC              GCTCTTC(1/4)
SspI          466  AATATT               AAT^ATT
StyI          116  CCAAGG               C^C[AT][AT]GG
TfiI          675  GAATC                G^A[AT]TC
XcmI          299  CCATTGTGATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         573  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcsI          452  AAATTC               [GA]^AATT[CT]
              767  GAATTT              
AfaI          258  GTAC                 GT^AC
              853  GTAC                
AlwI          306  GATCC                GGATC(4/5)
              574  GATCC               
ApoI          452  AAATTC               [GA]^AATT[CT]
              767  GAATTT              
BbvI          602  GCAGC                GCAGC(8/12)
              782  GCTGC               
BmyI          333  GGGCAC               G[AGT]GC[ACT]^C
              586  GAGCTC              
BsaJI         116  CCAAGG               C^C[ACGT][ACGT]GG
              737  CCTGGG              
BsiYI         683  CCTTCAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              732  CCCATCCTGGG         
BslI          683  CCTTCAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              732  CCCATCCTGGG         
Bsp1286I      333  GGGCAC               G[AGT]GC[ACT]^C
              586  GAGCTC              
BstF5I         32  CATCC                GGATG(2/0)
              734  CATCC               
Cac8I          72  GCTGGC               GC[ACGT]^[ACGT]GC
              174  GCTGGC              
DdeI          584  CTGAG                C^T[ACGT]AG
              756  CTCAG               
DpnII         306  GATC                 ^GATC
              574  GATC                
Fnu4HI        602  GCAGC                GC^[ACGT]GC
              782  GCTGC               
FokI           32  CATCC                GGATG(9/13)
              734  CATCC               
HphI           28  TCACC                GGTGA(8/7)
              513  TCACC               
ItaI          602  GCAGC                GC^[ACGT]GC
              782  GCTGC               
MaeII         110  ACGT                 A^CGT
              403  ACGT                
MboI          306  GATC                 ^GATC
              574  GATC                
MslI           32  CATCCAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              338  CATACGAGTG          
NdeII         306  GATC                 ^GATC
              574  GATC                
NlaIII        240  CATG                 CATG^
              642  CATG                
RsaI          258  GTAC                 GT^AC
              853  GTAC                
Sau3AI        306  GATC                 ^GATC
              574  GATC                
SfaNI         809  GATGC                GCATC(5/9)
              188  GCATC               
TseI          602  GCAGC                G^C[AT]GC
              782  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AluI        BsrI        BssKI       BstNI       Cfr13I      
EcoRII      HaeIII      MaeIII      MboII       MnlI        
MseI        MvaI        Sau96I      ScrFI       Tru9I       
Tsp45I      Tsp509I     
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