Restriction map for pF1KE2391
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BbrPI       BbvCI       BcgI        
BclI        BfaI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaWI       
BseAI       BsiWI       BsmBI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BsrDI       BsrGI       
BssHII      BssSI       BstAPI      BstBI       BstUI       
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco81I      EcoRI       
EcoRV       EcoT22I     FbaI        FspI        HindIII     
HinfI       KpnI        KspI        MaeI        MamI        
MfeI        MluI        MroI        MunI        MvnI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        SspBI       SspI        
StuI        SwaI        TfiI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AcsI          280  GAATTT               [GA]^AATT[CT]
AflIII       1079  ACATGT               A^C[GA][CT]GT
Aor51HI      1541  AGCGCT               AGC^GCT
ApaI         1115  GGGCCC               GGGCC^C
ApoI          280  GAATTT               [GA]^AATT[CT]
Asp700I      1044  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI         1235  GGCGCC               GGCGC^C
BciVI        1144  GGATAC               GTATCC(6/5)
BcnI          312  CCCGG                CC^[GC]GG
BglII         371  AGATCT               A^GATCT
BsiEI         646  CGGCCG               CG[GA][CT]^CG
BsmI          270  GAATGC               GAATGC(1/-1)
BspLU11I     1079  ACATGT               A^CATGT
BspMI         429  ACCTGC               ACCTGC(4/8)
Bst1107I      519  GTATAC               GTA^TAC
BstEII        957  GGTCACC              G^GT[ACGT]ACC
BstPI         957  GGTCACC              G^GT[ACGT]ACC
BstZ17I       519  GTATAC               GTA^TAC
EagI          646  CGGCCG               C^GGCCG
EclXI         646  CGGCCG               C^GGCCG
Eco47III     1541  AGCGCT               AGC^GCT
Eco52I        646  CGGCCG               C^GGCCG
EcoNI         546  CCTGAAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       957  GGTCACC              G^GT[ACGT]ACC
FseI          647  GGCCGGCC             GGCCGG^CC
HpaI         1336  GTTAAC               GTT^AAC
KasI         1235  GGCGCC               G^GCGCC
MseI         1337  TTAA                 T^TAA
NarI         1235  GGCGCC               GG^CGCC
NciI          312  CCCGG                CC^[GC]GG
NspI         1079  ACATGT               [GA]CATG^[CT]
PflMI         224  CCAATGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1511  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI        1431  GACGCCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1468  CTGCAG               CTGCA^G
SbfI         1467  CCTGCAGG             CCTGCA^GG
ScaI         1166  AGTACT               AGT^ACT
SexAI         794  ACCTGGT              A^CC[AT]GGT
SfaNI         556  GATGC                GCATC(5/9)
SfcI         1468  CTGCAG               C^T[GA][CT]AG
SfoI         1235  GGCGCC               GGC^GCC
Sse8387I     1467  CCTGCAGG             CCTGCA^GG
Tru9I        1337  TTAA                 T^TAA
Van91I        224  CCAATGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1044  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI          397  GTCTAC               GT^[AC][GT]AC
              519  GTATAC              
AcyI         1235  GGCGCC               G[GA]^CG[CT]C
             1431  GACGCC              
AfaI          417  GTAC                 GT^AC
             1167  GTAC                
BpmI          343  CTGGAG               CTGGAG(16/14)
             1054  CTGGAG              
BsaHI        1235  GGCGCC               G[GA]^CG[CT]C
             1431  GACGCC              
BsaI          211  GAGACC               GGTCTC(1/5)
             1057  GAGACC              
BsmAI         211  GAGAC                GTCTC(1/5)
             1057  GAGAC               
BstXI         902  CCACATCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              962  CCATGGTTGTGG        
BstYI         371  AGATCT               [GA]^GATC[CT]
             1460  GGATCT              
HaeII        1235  GGCGCC               [GA]GCGC^[CT]
             1541  AGCGCT              
HgaI         1431  GACGC                GACGC(5/10)
              891  GCGTC               
Hin1I        1235  GGCGCC               G[GA]^CG[CT]C
             1431  GACGCC              
MflI          371  AGATCT               [GA]^GATC[CT]
             1460  GGATCT              
NaeI          648  GCCGGC               GCC^GGC
             1253  GCCGGC              
NcoI          908  CCATGG               C^CATGG
              962  CCATGG              
NgoMIV        648  GCCGGC               G^CCGGC
             1253  GCCGGC              
RsaI          417  GTAC                 GT^AC
             1167  GTAC                
SacI          121  GAGCTC               GAGCT^C
             1069  GAGCTC              
SapI          939  GCTCTTC              GCTCTTC(1/4)
             1047  GCTCTTC             
TaqI          479  TCGA                 T^CGA
             1202  TCGA                
Tsp509I       281  AATT                 ^AATT
             1360  AATT                
TthHB8I       479  TCGA                 T^CGA
             1202  TCGA                
XhoII         371  AGATCT               [GA]^GATC[CT]
             1460  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AciI        AluI        AlwI        AspHI       AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BmyI        BpuAI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmFI       Bsp1286I    
BsrBI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HincII      HindII      HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PvuII       Sau3AI      Sau96I      
ScrFI       SmlI        StyI        TseI        Tsp45I      
TspRI       XcmI        
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