Restriction map for pF1KE1246
 (Circular) (Six-base) MAPSORT of: pF1KE1246.seq  Check: 4429  from: 1  to: 4559



pF1KE1246  4559 bp

 With 182 enzymes: SgfI * 

                        September 29, 2011 13:09  ..

AccI GT'mk_AC

Cuts at:   1527    1597    1527 
   Size:         70    4489
 
Acc65I G'GTAC_C

Cuts at:   1575    1575 
   Size:       4559
 
AclI AA'CG_TT

Cuts at:   4399    4399 
   Size:       4559
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    470    2384    2816    3563    3831     470 
   Size:       1914     432     747     268    1198

  Fragments arranged by size: 

                1914    1198     747     432     268
 
AflIII A'CryG_T

Cuts at:   1911    3015    3992    1911 
   Size:       1104     977    2478

  Fragments arranged by size: 

                2478    1104     977
 
AgeI A'CCGG_T

Cuts at:   2947    2947 
   Size:       4559
 
AhdI GACnn_n'nnGTC

Cuts at:   1413    1413 
   Size:       4559
 
AleI CACnn'nnGTG

Cuts at:    562    1087     562 
   Size:        525    4034
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    238     238 
   Size:       4559
 
AlwNI CAG_nnn'CTG

Cuts at:    870    1864    3431     870 
   Size:        994    1567    1998

  Fragments arranged by size: 

                1998    1567     994
 
ApaLI G'TGCA_C

Cuts at:   3329    3329 
   Size:       4559
 
ApoI r'AATT_y

Cuts at:    473    1563    3912    4168     473 
   Size:       1090    2349     256     864

  Fragments arranged by size: 

                2349    1090     864     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4559
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4559
 
AvaI C'yCGr_G

Cuts at:    785    1351    1579     785 
   Size:        566     228    3765

  Fragments arranged by size: 

                3765     566     228
 
BamHI G'GATC_C

Cuts at:   1584    1584 
   Size:       4559
 
BanI G'GyrC_C

Cuts at:    500     682    1575    1989    2237    2272     500 
   Size:        182     893     414     248      35    2787

  Fragments arranged by size: 

                2787     893     414     248     182      35
 
BanII G_rGCy'C

Cuts at:    116     169    1573    2603     116 
   Size:         53    1404    1030    2072

  Fragments arranged by size: 

                2072    1404    1030      53
 
BbeI G_GCGC'C

Cuts at:    504    1993    2241     504 
   Size:       1489     248    2822

  Fragments arranged by size: 

                2822    1489     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    242    1280    1473     242 
   Size:       1038     193    3328

  Fragments arranged by size: 

                3328    1038     193
 
BciVI GTATCCnnnnn_n'

Cuts at:   2457    3218    4559    2457 
   Size:        761    1341    2457

  Fragments arranged by size: 

                2457    1341     761
 
BclI T'GATC_A

Cuts at:    773    1340    2079     773 
   Size:        567     739    3253

  Fragments arranged by size: 

                3253     739     567
 
BglII A'GATC_T

Cuts at:    354     494    2074    4111     354 
   Size:        140    1580    2037     802

  Fragments arranged by size: 

                2037    1580     802     140
 
BlpI GC'TnA_GC

Cuts at:    147    1671     147 
   Size:       1524    3035
 
Bme1580I G_kGCm'C

Cuts at:    685    1058    2184    2277    3333     685 
   Size:        373    1126      93    1056    1911

  Fragments arranged by size: 

                1911    1126    1056     373      93
 
BmgBI CAC'GTC

Cuts at:   1043    1043 
   Size:       4559
 
BmrI ACTGGGnnnn_n'

Cuts at:   1182    1771    1852    2187    4331    1182 
   Size:        589      81     335    2144    1410

  Fragments arranged by size: 

                2144    1410     589     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2926    2926 
   Size:       4559
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    587     587 
   Size:       4559
 
Bpu10I CC'TnA_GC

Cuts at:    742     742 
   Size:       4559
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1735    2614    3106    3404    1735 
   Size:        879     492     298    2890

  Fragments arranged by size: 

                2890     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    172    1387    4263     172 
   Size:       1215    2876     468

  Fragments arranged by size: 

                2876    1215     468
 
BsaAI yAC'GTr

Cuts at:    593    1912     593 
   Size:       1319    3240
 
BsaBI GATnn'nnATC

Cuts at:      6    2073    4110       6 
   Size:       2067    2037     455

  Fragments arranged by size: 

                2067    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    501    1990    2238    2940    4436     501 
   Size:       1489     248     702    1496     624

  Fragments arranged by size: 

                1496    1489     702     624     248
 
BsaWI w'CCGG_w

Cuts at:   1748    2269    2947    3221    3368    3779    1748 
   Size:        521     678     274     147     411    2528

  Fragments arranged by size: 

                2528     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    115    1462     115 
   Size:       1347    3212
 
BseYI C'CCAG_C

Cuts at:    367     678    1150    1984    2742    3319    3815     367 
   Size:        311     472     834     758     577     496    1111

  Fragments arranged by size: 

                1111     834     758     577     496     472     311
 
BsiEI CG_ry'CG

Cuts at:    104    2101    2147    3355     104 
   Size:       1997      46    1208    1308

  Fragments arranged by size: 

                1997    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    569    1573    2351    2541    3333     569 
   Size:       1004     778     190     792    1795

  Fragments arranged by size: 

                1795    1004     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    116     169     569     685    1058    1573    2184    2277    2351 
   Size:         53     400     116     373     515     611      93      74

Cuts at:   2351    2541    2603    3333     116 
   Size:        190      62     730    1342

  Fragments arranged by size: 

                1342     730     611     515     400     373     190     116
                  93      74      62      53
 
BspEI T'CCGG_A

Cuts at:   3779    3779 
   Size:       4559
 
BspHI T'CATG_A

Cuts at:   3735    3735 
   Size:       4559
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    953    1513    1609    1823    2125    2506     953 
   Size:        560      96     214     302     381    3006

  Fragments arranged by size: 

                3006     560     381     302     214      96
 
BsrBI CCG'CTC

Cuts at:     50     761    1145    2851    2905    4389    4554      50 
   Size:        711     384    1706      54    1484     165      55

  Fragments arranged by size: 

                1706    1484     711     384     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    424    1097    1457    2471     424 
   Size:        673     360    1014    2512

  Fragments arranged by size: 

                2512    1014     673     360
 
BsrFI r'CCGG_y

Cuts at:   2557    2738    2947    2557 
   Size:        181     209    4169

  Fragments arranged by size: 

                4169     209     181
 
BssSI C'ACGA_G

Cuts at:   2830    3188    2830 
   Size:        358    4201
 
BstAPI GCAn_nnn'nTGC

Cuts at:    870    1936     870 
   Size:       1066    3493
 
BstBI TT'CG_AA

Cuts at:   2920    2920 
   Size:       4559
 
BstEII G'GTnAC_C

Cuts at:    258     258 
   Size:       4559
 
BstYI r'GATC_y

Cuts at:      1     354     494    1584    2051    2074    2409    2655    3667 
   Size:        353     140    1090     467      23     335     246    1012

Cuts at:   3667    3753    3765    4111       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1090    1012     467     449     353     346     335     246
                 140      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     263    1085    1319     107 
   Size:        156     822     234    3347

  Fragments arranged by size: 

                3347     822     234     156
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1371    2509    2690    2853    1371 
   Size:       1138     181     163    3077

  Fragments arranged by size: 

                3077    1138     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1802    1802 
   Size:       4559
 
DraI TTT'AAA

Cuts at:    204     615    1558     204 
   Size:        411     943    3205

  Fragments arranged by size: 

                3205     943     411
 
DraIII CAC_nnn'GTG

Cuts at:    804     804 
   Size:       4559
 
DrdI GACnn_nn'nnGTC

Cuts at:    703    1401    2265    3123     703 
   Size:        698     864     858    2139

  Fragments arranged by size: 

                2139     864     858     698
 
EaeI y'GGCC_r

Cuts at:    226     582     710    2144    2318    2709    2736    4081     226 
   Size:        356     128    1434     174     391      27    1345     704

  Fragments arranged by size: 

                1434    1345     704     391     356     174     128      27
            
 
EagI C'GGCC_G

Cuts at:   2144    2144 
   Size:       4559
 
EarI CTCTTCn'nnn_

Cuts at:    919    2582    2792     919 
   Size:       1663     210    2686

  Fragments arranged by size: 

                2686    1663     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2767    3077    3223    4369    2767 
   Size:        310     146    1146    2957

  Fragments arranged by size: 

                2957    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1571    1571 
   Size:       4559
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    470     587    2384    2816    3563    3831     470 
   Size:        117    1797     432     747     268    1198

  Fragments arranged by size: 

                1797    1198     747     432     268     117
 
EcoO109I rG'GnC_Cy

Cuts at:   1309    1698    1309 
   Size:        389    4170
 
EcoRI G'AATT_C

Cuts at:   1563    1563 
   Size:       4559
 
EcoRV GAT'ATC

Cuts at:   1284    1284 
   Size:       4559
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2578    2578 
   Size:       4559
 
FspI TGC'GCA

Cuts at:   2340    2340 
   Size:       4559
 
HaeII r_GCGC'y

Cuts at:    504     870    1993    2241    3263    4242     504 
   Size:        366    1123     248    1022     979     821

  Fragments arranged by size: 

                1123    1022     979     821     366     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1399    1431    2401    2431    2433    2463    1399 
   Size:         32     970      30       2      30    3495

  Fragments arranged by size: 

                3495     970      32      30      30       2
 
HincII GTy'rAC

Cuts at:    891    1528    1598     891 
   Size:        637      70    3852

  Fragments arranged by size: 

                3852     637      70
 
KasI G'GCGC_C

Cuts at:    500    1989    2237     500 
   Size:       1489     248    2822

  Fragments arranged by size: 

                2822    1489     248
 
KpnI G_GTAC'C

Cuts at:   1579    1579 
   Size:       4559
 
MluI A'CGCG_T

Cuts at:   3992    3992 
   Size:       4559
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3230    3414    3230 
   Size:        184    4375
 
MscI TGG'CCA

Cuts at:    584     712    2320     584 
   Size:        128    1608    2823

  Fragments arranged by size: 

                2823    1608     128
 
MslI CAynn'nnrTG

Cuts at:    292     562    1087    2548    2675     292 
   Size:        270     525    1461     127    2176

  Fragments arranged by size: 

                2176    1461     525     270     127
 
MspA1I CmG'CkG

Cuts at:    151     276     371    1670    1984    2344    3357    3602     151 
   Size:        125      95    1299     314     360    1013     245    1108

  Fragments arranged by size: 

                1299    1108    1013     360     314     245     125      95
            
 
NaeI GCC'GGC

Cuts at:   2740    2740 
   Size:       4559
 
NarI GG'CG_CC

Cuts at:    501    1990    2238     501 
   Size:       1489     248    2822

  Fragments arranged by size: 

                2822    1489     248
 
NcoI C'CATG_G

Cuts at:    107    1085    1319     107 
   Size:        978     234    3347

  Fragments arranged by size: 

                3347     978     234
 
NgoMIV G'CCGG_C

Cuts at:   2738    2738 
   Size:       4559
 
NspI r_CATG'y

Cuts at:   1077    1612    2112    3019    1077 
   Size:        535     500     907    2617

  Fragments arranged by size: 

                2617     907     535     500
 
PciI A'CATG_T

Cuts at:   3015    3015 
   Size:       4559
 
PflMI CCAn_nnn'nTGG

Cuts at:    252     252 
   Size:       4559
 
PmeI GTTT'AAAC

Cuts at:   1558    1558 
   Size:       4559
 
PmlI CAC'GTG

Cuts at:    593     593 
   Size:       4559
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3698    3730    3698 
   Size:         32    4527
 
PstI C_TGCA'G

Cuts at:    275     410     575     670    1606    2291     275 
   Size:        135     165      95     936     685    2543

  Fragments arranged by size: 

                2543     936     685     165     135      95
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4559
 
PvuII CAG'CTG

Cuts at:    151     276     371    1984    2344     151 
   Size:        125      95    1613     360    2366

  Fragments arranged by size: 

                2366    1613     360     125      95
 
RsrII CG'GwC_CG

Cuts at:   2754    2754 
   Size:       4559
 
SacI G_AGCT'C

Cuts at:   1573    1573 
   Size:       4559
 
SalI G'TCGA_C

Cuts at:   1526    1596    1526 
   Size:         70    4489
 
SapI GCTCTTCn'nnn_

Cuts at:    919    2582    2792     919 
   Size:       1663     210    2686

  Fragments arranged by size: 

                2686    1663     210
 
SbfI CC_TGCA'GG

Cuts at:   1606    1606 
   Size:       4559
 
ScaI AGT'ACT

Cuts at:   4076    4076 
   Size:       4559
 
SfcI C'TryA_G

Cuts at:     33     210     271     406     571     666    1602    2287    3280 
   Size:        177      61     135     165      95     936     685     993

Cuts at:   3280    3471      33 
   Size:        191    1121

  Fragments arranged by size: 

                1121     993     936     685     191     177     165     135
                  95      61
 
SfoI GGC'GCC

Cuts at:    502    1991    2239     502 
   Size:       1489     248    2822

  Fragments arranged by size: 

                2822    1489     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4559
 
SmaI CCC'GGG

Cuts at:    787    1581     787 
   Size:        794    3765
 
SmlI C'TyrA_G

Cuts at:   1714    2629    3121    3383    1714 
   Size:        915     492     262    2890

  Fragments arranged by size: 

                2890     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4559
 
SphI G_CATG'C

Cuts at:   1612    2112    1612 
   Size:        500    4059
 
StyI C'CwwG_G

Cuts at:    107    1085    1319    1693    2047     107 
   Size:        978     234     374     354    2619

  Fragments arranged by size: 

                2619     978     374     354     234
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    411     655     759    1164    1171    1332    2142    2275    2734 
   Size:        244     104     405       7     161     810     133     459

Cuts at:   2734    2943     411 
   Size:        209    2027

  Fragments arranged by size: 

                2027     810     459     405     244     209     161     133
                 104       7
 
TatI w'GTAC_w

Cuts at:    575    1382    4074     575 
   Size:        807    2692    1060

  Fragments arranged by size: 

                2692    1060     807
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    630     662    4076    4108     630 
   Size:         32    3414      32    1081

  Fragments arranged by size: 

                3414    1081      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2356    2356 
   Size:       4559
 
XbaI T'CTAG_A

Cuts at:   1590    1590 
   Size:       4559
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2054    2054 
   Size:       4559
 
XmaI C'CCGG_G

Cuts at:    785    1579     785 
   Size:        794    3765
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI     AleI
     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI    PflMI     PmeI     PmlI
     PpiI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AloI     ApaI     AscI    AvrII
     BaeI    BbvCI     BcgI    BfrBI     BglI     BmtI    BsaXI     BsgI
    BsiWI     BsmI    BsmBI    BsrGI   BssHII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI    EcoNI     FseI    FspAI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI
     XhoI     XmnI     ZraI
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