Restriction map for pF1KE2347
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AflIII      
AgeI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspI        AviII       
BamHI       BbrPI       BbsI        BciVI       BclI        
BfrI        BglII       BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaI        BsiWI       BsmBI       
BspDI       BspHI       BspLU11I    BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
Eco47III    EcoNI       EcoO65I     EcoRI       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HpaI        KpnI        MamI        MfeI        MluI        
MunI        NcoI        NdeI        NotI        NruI        
NsiI        NspI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        Tth111I     


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI          697  GTCTAC               GT^[AC][GT]AC
AhdI         1713  GACAGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          135  CCTGAGG              CC^T[ACGT]AGG
Asp700I      1455  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1713  GACAGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII         645  CCTAGG               C^CTAGG
BcgI         1122  CGAGAAATATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI          645  CCTAGG               C^CTAGG
BsiEI         452  CGGCCG               CG[GA][CT]^CG
BsmAI         928  GTCTC                GTCTC(1/5)
Bsp1407I     1241  TGTACA               T^GTACA
BspMI         299  GCAGGT               ACCTGC(4/8)
BsrBI         207  GAGCGG               CCGCTC(-3/-3)
BsrGI        1241  TGTACA               T^GTACA
Bsu36I        135  CCTGAGG              CC^T[ACGT]AGG
EagI          452  CGGCCG               C^GGCCG
Eam1105I     1713  GACAGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         452  CGGCCG               C^GGCCG
Eco52I        452  CGGCCG               C^GGCCG
Eco81I        135  CCTGAGG              CC^T[ACGT]AGG
EcoRV        1001  GATATC               GAT^ATC
HgaI          245  GCGTC                GACGC(5/10)
HindIII      1458  AAGCTT               A^AGCTT
KspI           92  CCGCGG               CCGC^GG
NheI         1056  GCTAGC               G^CTAGC
PaeR7I         34  CTCGAG               C^TCGAG
PflMI        1809  CCAGAGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         112  GGGTCCC              [GA]G^G[AT]CC[CT]
PstI          297  CTGCAG               CTGCA^G
SacII          92  CCGCGG               CCGC^GG
SapI         2146  GAAGAGC              GCTCTTC(1/4)
SmlI           34  CTCGAG               C^T[CT][GA]AG
SspBI        1241  TGTACA               T^GTACA
StuI          392  AGGCCT               AGG^CCT
Van91I       1809  CCAGAGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2025  TCTAGA               T^CTAGA
XcmI          602  CCAACCCCACTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI           34  CTCGAG               C^TCGAG
XmnI         1455  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccIII       1213  TCCGGA               T^CCGGA
             1847  TCCGGA              
AcsI         1851  GAATTT               [GA]^AATT[CT]
             1958  AAATTC              
AlwI         1700  GATCC                GGATC(4/5)
             1558  GGATC               
ApaI          163  GGGCCC               GGGCC^C
              890  GGGCCC              
ApoI         1851  GAATTT               [GA]^AATT[CT]
             1958  AAATTC              
BsaWI        1213  TCCGGA               [AT]^CCGG[AT]
             1847  TCCGGA              
BseAI        1213  TCCGGA               T^CCGGA
             1847  TCCGGA              
BsgI          836  CTGCAC               GTGCAG(16/14)
             1379  GTGCAG              
BsmI         2088  GAATGC               GAATGC(1/-1)
             1174  GCATTC              
BspEI        1213  TCCGGA               T^CCGGA
             1847  TCCGGA              
BsrDI         981  CATTGC               GCAATG(2/0)
             1761  CATTGC              
BssHII        193  GCGCGC               G^CGCGC
              342  GCGCGC              
BstAPI        367  GCAGCCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1978  GCAACCAGTGC         
BstYI        1558  GGATCT               [GA]^GATC[CT]
             1699  AGATCC              
DsaI           92  CCGCGG               C^C[GA][CT]GG
             1280  CCGTGG              
EarI         1293  CTCTTC               CTCTTC(1/4)
             2146  GAAGAG              
HphI         1285  GGTGA                GGTGA(8/7)
              931  TCACC               
Ksp632I      1293  CTCTTC               CTCTTC(1/4)
             2146  GAAGAG              
MaeII        1068  ACGT                 A^CGT
             1487  ACGT                
MflI         1558  GGATCT               [GA]^GATC[CT]
             1699  AGATCC              
MroI         1213  TCCGGA               T^CCGGA
             1847  TCCGGA              
MslI          909  CATCAGTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1275  CATGACCGTG          
NaeI          141  GCCGGC               GCC^GGC
              408  GCCGGC              
NgoMIV        141  GCCGGC               G^CCGGC
              408  GCCGGC              
PshAI        1017  GACCAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1669  GACATCCGTC          
PvuII         294  CAGCTG               CAG^CTG
              515  CAGCTG              
SacI         1344  GAGCTC               GAGCT^C
             1399  GAGCTC              
SfaNI        1993  GATGC                GCATC(5/9)
              908  GCATC               
SfcI          297  CTGCAG               C^T[GA][CT]AG
              618  CTACAG              
TfiI         1033  GATTC                G^A[AT]TC
             1950  GAATC               
XhoII        1558  GGATCT               [GA]^GATC[CT]
             1699  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbeI        BbvCI       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       ItaI        KasI        
MaeI        MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MseI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfoI        SmaI        
StyI        TaqI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XmaCI       XmaI        
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