Restriction map for
pF1KB9400
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI AhdI ApoI
AscI AseI AsnI Asp700I AspEI
AspI BbeI BbrPI BbvCI BfrI
BglII BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV EcoT22I FseI
Hin1I HincII HindII HpaI KasI
KspI MamI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 1661 CGCG CG^CG
Alw44I 2157 GTGCAC G^TGCAC
AocI 1636 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2129 AGCGCT AGC^GCT
ApaI 908 GGGCCC GGGCC^C
ApaLI 2157 GTGCAC G^TGCAC
AviII 557 TGCGCA TGC^GCA
AvrII 1045 CCTAGG C^CTAGG
BamHI 941 GGATCC G^GATCC
BbsI 81 GTCTTC GAAGAC(2/6)
BcgI 2402 GCAGCGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 543 GTATCC GTATCC(6/5)
BclI 2051 TGATCA T^GATCA
BlnI 1045 CCTAGG C^CTAGG
BpuAI 81 GTCTTC GAAGAC(2/6)
BsaAI 2437 TACGTG [CT]AC^GT[GA]
BsmBI 520 GAGACG CGTCTC(1/5)
BsmI 1096 GAATGC GAATGC(1/-1)
BsrDI 714 CATTGC GCAATG(2/0)
BsrFI 827 GCCGGC [GA]^CCGG[CT]
BssSI 2502 CACGAG CACGAG(-5/-1)
BstAPI 1091 GCAGAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 174 GGTGACC G^GT[ACGT]ACC
BstPI 174 GGTGACC G^GT[ACGT]ACC
BstUI 1661 CGCG CG^CG
Bsu36I 1636 CCTGAGG CC^T[ACGT]AGG
Cfr10I 827 GCCGGC [GA]^CCGG[CT]
DraIII 1276 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2129 AGCGCT AGC^GCT
Eco81I 1636 CCTGAGG CC^T[ACGT]AGG
EcoO65I 174 GGTGACC G^GT[ACGT]ACC
FauI 1659 CCCGC CCCGC(4/6)
FbaI 2051 TGATCA T^GATCA
FspI 557 TGCGCA TGC^GCA
HindIII 1103 AAGCTT A^AGCTT
MaeII 2438 ACGT A^CGT
MseI 1316 TTAA T^TAA
MvnI 1661 CGCG CG^CG
NaeI 827 GCCGGC GCC^GGC
NgoMIV 827 GCCGGC G^CCGGC
PpuMI 1573 GGGACCT [GA]G^G[AT]CC[CT]
SacI 382 GAGCTC GAGCT^C
SapI 2298 GCTCTTC GCTCTTC(1/4)
SmlI 2152 CTTGAG C^T[CT][GA]AG
TaqI 851 TCGA T^CGA
Tru9I 1316 TTAA T^TAA
Tsp509I 1353 AATT ^AATT
TthHB8I 851 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
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Acc65I 296 GGTACC G^GTACC
1995 GGTACC
AccI 1246 GTCTAC GT^[AC][GT]AC
2344 GTCTAC
Asp718I 296 GGTACC G^GTACC
1995 GGTACC
BalI 836 TGGCCA TGG^CCA
971 TGGCCA
BseRI 2209 CTCCTC GAGGAG(10/8)
250 GAGGAG
HaeII 2129 AGCGCT [GA]GCGC^[CT]
2507 GGCGCT
HgaI 522 GACGC GACGC(5/10)
2342 GCGTC
KpnI 296 GGTACC GGTAC^C
1995 GGTACC
MluNI 836 TGGCCA TGG^CCA
971 TGGCCA
MscI 836 TGGCCA TGG^CCA
971 TGGCCA
NcoI 593 CCATGG C^CATGG
1191 CCATGG
PflMI 1203 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1515 CCAGCGAGTGG
PvuII 301 CAGCTG CAG^CTG
2068 CAGCTG
SexAI 581 ACCAGGT A^CC[AT]GGT
1469 ACCAGGT
SmaI 937 CCCGGG CCC^GGG
1025 CCCGGG
Van91I 1203 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1515 CCAGCGAGTGG
XcmI 281 CCAACATCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
824 CCAGCCGGCAGTTGG
XmaCI 937 CCCGGG C^CCGGG
1025 CCCGGG
XmaI 937 CCCGGG C^CCGGG
1025 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TseI Tsp45I TspRI XhoII