(Linear) (Six-base) MAPSORT of: KIBB9400.seq Check: 4384 from: 1 to: 2523 KIBB9400.orf With 182 enzymes: SgfI * December 2, 2013 11:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 144 2523 Size: 144 2379 AccI GT'mk_AC Cuts at: 0 1247 2345 2523 Size: 1247 1098 178 Fragments arranged by size: 1247 1098 178 Acc65I G'GTAC_C Cuts at: 0 296 1995 2523 Size: 296 1699 528 Fragments arranged by size: 1699 528 296 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1021 2274 2523 Size: 1021 1253 249 Fragments arranged by size: 1253 1021 249 AfeI AGC'GCT Cuts at: 0 2131 2523 Size: 2131 392 AleI CACnn'nnGTG Cuts at: 0 808 931 1669 1783 2523 Size: 808 123 738 114 740 Fragments arranged by size: 808 740 738 123 114 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2377 2523 Size: 2377 146 AlwNI CAG_nnn'CTG Cuts at: 0 681 1209 1499 2523 Size: 681 528 290 1024 Fragments arranged by size: 1024 681 528 290 ApaI G_GGCC'C Cuts at: 0 912 2523 Size: 912 1611 ApaLI G'TGCA_C Cuts at: 0 2157 2523 Size: 2157 366 AvaI C'yCGr_G Cuts at: 0 169 937 1025 2523 Size: 169 768 88 1498 Fragments arranged by size: 1498 768 169 88 AvrII C'CTAG_G Cuts at: 0 1045 2523 Size: 1045 1478 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1979 2012 2523 Size: 1979 33 511 Fragments arranged by size: 1979 511 33 BamHI G'GATC_C Cuts at: 0 941 2523 Size: 941 1582 BanI G'GyrC_C Cuts at: 0 296 489 933 1473 1511 1632 1769 1995 Size: 296 193 444 540 38 121 137 226 Cuts at: 1995 2523 Size: 528 Fragments arranged by size: 540 528 444 296 226 193 137 121 38 BanII G_rGCy'C Cuts at: 0 386 481 912 1066 1831 2523 Size: 386 95 431 154 765 692 Fragments arranged by size: 765 692 431 386 154 95 BbsI GAAGACnn'nnnn_ Cuts at: 0 74 2523 Size: 74 2449 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2391 2425 2523 Size: 2391 34 98 Fragments arranged by size: 2391 98 34 BciVI GTATCCnnnnn_n' Cuts at: 0 554 2523 Size: 554 1969 BclI T'GATC_A Cuts at: 0 2051 2523 Size: 2051 472 BglI GCCn_nnn'nGGC Cuts at: 0 26 1061 1859 2523 Size: 26 1035 798 664 Fragments arranged by size: 1035 798 664 26 BlpI GC'TnA_GC Cuts at: 0 475 526 2058 2389 2523 Size: 475 51 1532 331 134 Fragments arranged by size: 1532 475 331 134 51 Bme1580I G_kGCm'C Cuts at: 0 494 912 938 1514 1562 1635 2161 2523 Size: 494 418 26 576 48 73 526 362 Fragments arranged by size: 576 526 494 418 362 73 48 26 BmrI ACTGGGnnnn_n' Cuts at: 0 938 2523 Size: 938 1585 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 130 415 1351 1713 2187 2523 Size: 130 285 936 362 474 336 Fragments arranged by size: 936 474 362 336 285 130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 703 900 1244 1400 2523 Size: 703 197 344 156 1123 Fragments arranged by size: 1123 703 344 197 156 Bpu10I CC'TnA_GC Cuts at: 0 2398 2523 Size: 2398 125 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2173 2523 Size: 2173 350 BsaI GGTCTCn'nnnn_ Cuts at: 0 386 583 1418 1432 2158 2523 Size: 386 197 835 14 726 365 Fragments arranged by size: 835 726 386 365 197 14 BsaAI yAC'GTr Cuts at: 0 2439 2523 Size: 2439 84 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1048 1078 1398 1428 2523 Size: 1048 30 320 30 1095 Fragments arranged by size: 1095 1048 320 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 265 2200 2523 Size: 265 1935 323 Fragments arranged by size: 1935 323 265 BseYI C'CCAG_C Cuts at: 0 53 963 1570 1824 2066 2383 2523 Size: 53 910 607 254 242 317 140 Fragments arranged by size: 910 607 317 254 242 140 53 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 694 1092 2523 Size: 694 398 1431 Fragments arranged by size: 1431 694 398 BsiHKAI G_wGCw'C Cuts at: 0 356 386 1694 2161 2477 2523 Size: 356 30 1308 467 316 46 Fragments arranged by size: 1308 467 356 316 46 30 BsmI GAATG_Cn' Cuts at: 0 1102 2523 Size: 1102 1421 BsmBI CGTCTCn'nnnn_ Cuts at: 0 514 2523 Size: 514 2009 Bsp1286I G_dGCh'C Cuts at: 0 356 386 481 494 912 938 1066 1514 Size: 356 30 95 13 418 26 128 448 Cuts at: 1514 1562 1635 1694 1831 2161 2477 2523 Size: 48 73 59 137 330 316 46 Fragments arranged by size: 448 418 356 330 316 137 128 95 73 59 48 46 30 26 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 144 1660 1831 2369 2523 Size: 144 1516 171 538 154 Fragments arranged by size: 1516 538 171 154 144 BsrDI GCAATG_nn' Cuts at: 0 713 2523 Size: 713 1810 BsrFI r'CCGG_y Cuts at: 0 827 2523 Size: 827 1696 BssSI C'ACGA_G Cuts at: 0 2502 2523 Size: 2502 21 BstAPI GCAn_nnn'nTGC Cuts at: 0 1097 2523 Size: 1097 1426 BstEII G'GTnAC_C Cuts at: 0 174 2523 Size: 174 2349 BstXI CCAn_nnnn'nTGG Cuts at: 0 697 810 952 1615 2035 2175 2379 2523 Size: 697 113 142 663 420 140 204 144 Fragments arranged by size: 697 663 420 204 144 142 140 113 BstYI r'GATC_y Cuts at: 0 629 707 941 1271 1329 2523 Size: 629 78 234 330 58 1194 Fragments arranged by size: 1194 629 330 234 78 58 Bsu36I CC'TnA_GG Cuts at: 0 1637 2523 Size: 1637 886 BtgI C'CryG_G Cuts at: 0 266 593 857 1191 2028 2333 2523 Size: 266 327 264 334 837 305 190 Fragments arranged by size: 837 334 327 305 266 264 190 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 770 2523 Size: 770 1753 BtsI GCAGTG_nn' Cuts at: 0 431 654 775 1539 2107 2523 Size: 431 223 121 764 568 416 Fragments arranged by size: 764 568 431 416 223 121 DraIII CAC_nnn'GTG Cuts at: 0 1281 2523 Size: 1281 1242 EaeI y'GGCC_r Cuts at: 0 509 836 971 2336 2369 2523 Size: 509 327 135 1365 33 154 Fragments arranged by size: 1365 509 327 154 135 33 EarI CTCTTCn'nnn_ Cuts at: 0 229 976 1619 2305 2523 Size: 229 747 643 686 218 Fragments arranged by size: 747 686 643 229 218 EcoICRI GAG'CTC Cuts at: 0 384 2523 Size: 384 2139 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 703 900 1021 1244 1400 2274 2523 Size: 703 197 121 223 156 874 249 Fragments arranged by size: 874 703 249 223 197 156 121 EcoNI CCTnn'n_nnAGG Cuts at: 0 226 790 2245 2523 Size: 226 564 1455 278 Fragments arranged by size: 1455 564 278 226 EcoO109I rG'GnC_Cy Cuts at: 0 909 1041 1574 1905 2523 Size: 909 132 533 331 618 Fragments arranged by size: 909 618 533 331 132 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 412 1401 2523 Size: 412 989 1122 Fragments arranged by size: 1122 989 412 FspI TGC'GCA Cuts at: 0 559 2523 Size: 559 1964 HaeII r_GCGC'y Cuts at: 0 2133 2511 2523 Size: 2133 378 12 Fragments arranged by size: 2133 378 12 HindIII A'AGCT_T Cuts at: 0 1103 2523 Size: 1103 1420 KpnI G_GTAC'C Cuts at: 0 300 1999 2523 Size: 300 1699 524 Fragments arranged by size: 1699 524 300 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1986 2523 Size: 1986 537 MscI TGG'CCA Cuts at: 0 838 973 2523 Size: 838 135 1550 Fragments arranged by size: 1550 838 135 MslI CAynn'nnrTG Cuts at: 0 808 931 955 1669 1783 2523 Size: 808 123 24 714 114 740 Fragments arranged by size: 808 740 714 123 114 24 MspA1I CmG'CkG Cuts at: 0 303 2062 2070 2405 2523 Size: 303 1759 8 335 118 Fragments arranged by size: 1759 335 303 118 8 NaeI GCC'GGC Cuts at: 0 829 2523 Size: 829 1694 NcoI C'CATG_G Cuts at: 0 593 1191 2523 Size: 593 598 1332 Fragments arranged by size: 1332 598 593 NgoMIV G'CCGG_C Cuts at: 0 827 2523 Size: 827 1696 PasI CC'CwG_GG Cuts at: 0 291 2175 2523 Size: 291 1884 348 Fragments arranged by size: 1884 348 291 PflMI CCAn_nnn'nTGG Cuts at: 0 1209 1521 2523 Size: 1209 312 1002 Fragments arranged by size: 1209 1002 312 PfoI T'CCnGG_A Cuts at: 0 2314 2523 Size: 2314 209 PpuMI rG'GwC_Cy Cuts at: 0 1574 2523 Size: 1574 949 PspOMI G'GGCC_C Cuts at: 0 908 2523 Size: 908 1615 PstI C_TGCA'G Cuts at: 0 35 155 611 1093 2236 2523 Size: 35 120 456 482 1143 287 Fragments arranged by size: 1143 482 456 287 120 35 PvuII CAG'CTG Cuts at: 0 303 2070 2523 Size: 303 1767 453 Fragments arranged by size: 1767 453 303 SacI G_AGCT'C Cuts at: 0 386 2523 Size: 386 2137 SapI GCTCTTCn'nnn_ Cuts at: 0 2305 2523 Size: 2305 218 SexAI A'CCwGG_T Cuts at: 0 581 1469 2523 Size: 581 888 1054 Fragments arranged by size: 1054 888 581 SfcI C'TryA_G Cuts at: 0 31 151 607 1089 1599 2232 2523 Size: 31 120 456 482 510 633 291 Fragments arranged by size: 633 510 482 456 291 120 31 SmaI CCC'GGG Cuts at: 0 939 1027 2523 Size: 939 88 1496 Fragments arranged by size: 1496 939 88 SmlI C'TyrA_G Cuts at: 0 2152 2523 Size: 2152 371 SrfI GCCC'GGGC Cuts at: 0 1027 2523 Size: 1027 1496 StuI AGG'CCT Cuts at: 0 125 1050 1884 2485 2523 Size: 125 925 834 601 38 Fragments arranged by size: 925 834 601 125 38 StyI C'CwwG_G Cuts at: 0 387 593 1045 1191 1917 1991 2523 Size: 387 206 452 146 726 74 532 Fragments arranged by size: 726 532 452 387 206 146 74 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1692 2523 Size: 1692 831 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1048 1080 1398 1430 2523 Size: 1048 32 318 32 1093 Fragments arranged by size: 1093 1048 318 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 288 831 2523 Size: 288 543 1692 Fragments arranged by size: 1692 543 288 XmaI C'CCGG_G Cuts at: 0 937 1025 2523 Size: 937 88 1498 Fragments arranged by size: 1498 937 88 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AleI AlfI AlwNI ApaI ApaLI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII HindIII KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SapI SexAI SfcI SmaI SmlI SrfI StuI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AatII AclI AflII AflIII AgeI AhdI AloI ApoI AscI AseI AsiSI BbeI BbvCI BfrBI BglII BmgBI BmtI BsaBI BsaHI BsaWI BsiEI BsiWI BspEI BspHI BsrBI BsrGI BssHII BstBI BstZ17I ClaI CspCI DraI DrdI EagI EciI EcoRI EcoRV FseI FspAI Hin4I HincII HpaI KasI MfeI MluI NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TatI Tth111I XbaI XhoI XmnI ZraI