(Linear) (Six-base) MAPSORT of: KIEE2698.seq Check: 84 from: 1 to: 2310 KIEE2698.orf With 182 enzymes: SgfI * June 29, 2017 17:53 .. Acc65I G'GTAC_C Cuts at: 0 38 2310 Size: 38 2272 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 253 427 808 2105 2137 2310 Size: 253 174 381 1297 32 173 Fragments arranged by size: 1297 381 253 174 173 32 AfeI AGC'GCT Cuts at: 0 2103 2310 Size: 2103 207 AflIII A'CryG_T Cuts at: 0 1940 2310 Size: 1940 370 AleI CACnn'nnGTG Cuts at: 0 163 1174 1867 2310 Size: 163 1011 693 443 Fragments arranged by size: 1011 693 443 163 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 761 1006 1834 2310 Size: 761 245 828 476 Fragments arranged by size: 828 761 476 245 AlwNI CAG_nnn'CTG Cuts at: 0 99 361 429 606 1932 2310 Size: 99 262 68 177 1326 378 Fragments arranged by size: 1326 378 262 177 99 68 ApoI r'AATT_y Cuts at: 0 1060 1147 1219 1642 2310 Size: 1060 87 72 423 668 Fragments arranged by size: 1060 668 423 87 72 BamHI G'GATC_C Cuts at: 0 1138 2310 Size: 1138 1172 BanI G'GyrC_C Cuts at: 0 38 320 1810 2310 Size: 38 282 1490 500 Fragments arranged by size: 1490 500 282 38 BbsI GAAGACnn'nnnn_ Cuts at: 0 2125 2310 Size: 2125 185 BbvCI CC'TCA_GC Cuts at: 0 2238 2310 Size: 2238 72 BclI T'GATC_A Cuts at: 0 1313 2310 Size: 1313 997 BfrBI ATG'CAT Cuts at: 0 993 2310 Size: 993 1317 BglI GCCn_nnn'nGGC Cuts at: 0 364 2310 Size: 364 1946 BglII A'GATC_T Cuts at: 0 558 2310 Size: 558 1752 Bme1580I G_kGCm'C Cuts at: 0 1165 2310 Size: 1165 1145 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1386 2301 2310 Size: 1386 915 9 Fragments arranged by size: 1386 915 9 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 451 487 871 934 1790 2310 Size: 64 387 36 384 63 856 520 Fragments arranged by size: 856 520 387 384 64 63 36 Bpu10I CC'TnA_GC Cuts at: 0 1522 2238 2310 Size: 1522 716 72 Fragments arranged by size: 1522 716 72 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 304 520 1333 1812 2310 Size: 304 216 813 479 498 Fragments arranged by size: 813 498 479 304 216 BsaI GGTCTCn'nnnn_ Cuts at: 0 1318 1357 2310 Size: 1318 39 953 Fragments arranged by size: 1318 953 39 BsaAI yAC'GTr Cuts at: 0 690 2310 Size: 690 1620 BsaHI Gr'CG_yC Cuts at: 0 1025 1423 1853 2310 Size: 1025 398 430 457 Fragments arranged by size: 1025 457 430 398 BsaWI w'CCGG_w Cuts at: 0 452 2310 Size: 452 1858 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1788 1818 2310 Size: 1788 30 492 Fragments arranged by size: 1788 492 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 727 868 931 1315 1527 2053 2310 Size: 727 141 63 384 212 526 257 Fragments arranged by size: 727 526 384 257 212 141 63 BseYI C'CCAG_C Cuts at: 0 368 2310 Size: 368 1942 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 712 1291 2310 Size: 484 228 579 1019 Fragments arranged by size: 1019 579 484 228 BsiEI CG_ry'CG Cuts at: 0 907 2310 Size: 907 1403 BsiHKAI G_wGCw'C Cuts at: 0 1798 2310 Size: 1798 512 BsmI GAATG_Cn' Cuts at: 0 2044 2310 Size: 2044 266 Bsp1286I G_dGCh'C Cuts at: 0 1165 1798 2310 Size: 1165 633 512 Fragments arranged by size: 1165 633 512 BspEI T'CCGG_A Cuts at: 0 452 2310 Size: 452 1858 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 266 2158 2177 2310 Size: 266 1892 19 133 Fragments arranged by size: 1892 266 133 19 BsrBI CCG'CTC Cuts at: 0 972 1776 1782 2310 Size: 972 804 6 528 Fragments arranged by size: 972 804 528 6 BsrFI r'CCGG_y Cuts at: 0 902 1001 1021 2310 Size: 902 99 20 1289 Fragments arranged by size: 1289 902 99 20 BsrGI T'GTAC_A Cuts at: 0 894 2310 Size: 894 1416 BstAPI GCAn_nnn'nTGC Cuts at: 0 361 426 597 2310 Size: 361 65 171 1713 Fragments arranged by size: 1713 361 171 65 BstXI CCAn_nnnn'nTGG Cuts at: 0 1418 1995 2310 Size: 1418 577 315 Fragments arranged by size: 1418 577 315 BstYI r'GATC_y Cuts at: 0 468 558 1138 1898 2310 Size: 468 90 580 760 412 Fragments arranged by size: 760 580 468 412 90 Bsu36I CC'TnA_GG Cuts at: 0 1237 2310 Size: 1237 1073 BtgI C'CryG_G Cuts at: 0 404 1907 2310 Size: 404 1503 403 Fragments arranged by size: 1503 404 403 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 694 2310 Size: 694 1616 BtsI GCAGTG_nn' Cuts at: 0 359 620 2310 Size: 359 261 1690 Fragments arranged by size: 1690 359 261 EaeI y'GGCC_r Cuts at: 0 137 904 1511 2062 2310 Size: 137 767 607 551 248 Fragments arranged by size: 767 607 551 248 137 EagI C'GGCC_G Cuts at: 0 904 2310 Size: 904 1406 EarI CTCTTCn'nnn_ Cuts at: 0 451 724 2310 Size: 451 273 1586 Fragments arranged by size: 1586 451 273 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 253 427 451 487 808 871 934 Size: 64 189 174 24 36 321 63 63 Cuts at: 934 1790 2105 2137 2310 Size: 856 315 32 173 Fragments arranged by size: 856 321 315 189 174 173 64 63 63 36 32 24 EcoO109I rG'GnC_Cy Cuts at: 0 885 1518 1600 2310 Size: 885 633 82 710 Fragments arranged by size: 885 710 633 82 EcoRI G'AATT_C Cuts at: 0 1219 2310 Size: 1219 1091 HaeII r_GCGC'y Cuts at: 0 2105 2310 Size: 2105 205 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 102 134 264 296 1096 1128 1440 1472 Size: 102 32 130 32 800 32 312 32 Cuts at: 1472 1547 1579 2310 Size: 75 32 731 Fragments arranged by size: 800 731 312 130 102 75 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 835 2282 2310 Size: 835 1447 28 Fragments arranged by size: 1447 835 28 KpnI G_GTAC'C Cuts at: 0 42 2310 Size: 42 2268 MfeI C'AATT_G Cuts at: 0 1272 1490 2310 Size: 1272 218 820 Fragments arranged by size: 1272 820 218 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1220 1418 2310 Size: 1220 198 892 Fragments arranged by size: 1220 892 198 MslI CAynn'nnrTG Cuts at: 0 163 988 1174 1867 2310 Size: 163 825 186 693 443 Fragments arranged by size: 825 693 443 186 163 MspA1I CmG'CkG Cuts at: 0 606 1572 1886 1932 2310 Size: 606 966 314 46 378 Fragments arranged by size: 966 606 378 314 46 NdeI CA'TA_TG Cuts at: 0 132 2310 Size: 132 2178 NsiI A_TGCA'T Cuts at: 0 995 2310 Size: 995 1315 NspI r_CATG'y Cuts at: 0 157 555 987 1446 1944 2310 Size: 157 398 432 459 498 366 Fragments arranged by size: 498 459 432 398 366 157 PciI A'CATG_T Cuts at: 0 1940 2310 Size: 1940 370 PflMI CCAn_nnn'nTGG Cuts at: 0 1433 1908 2310 Size: 1433 475 402 Fragments arranged by size: 1433 475 402 PfoI T'CCnGG_A Cuts at: 0 503 1141 2310 Size: 503 638 1169 Fragments arranged by size: 1169 638 503 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 602 634 2310 Size: 602 32 1676 Fragments arranged by size: 1676 602 32 PpuMI rG'GwC_Cy Cuts at: 0 885 1518 1600 2310 Size: 885 633 82 710 Fragments arranged by size: 885 710 633 82 PshAI GACnn'nnGTC Cuts at: 0 1024 2310 Size: 1024 1286 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1732 1764 2310 Size: 1732 32 546 Fragments arranged by size: 1732 546 32 PstI C_TGCA'G Cuts at: 0 357 1218 2155 2310 Size: 357 861 937 155 Fragments arranged by size: 937 861 357 155 PvuII CAG'CTG Cuts at: 0 606 1572 1932 2310 Size: 606 966 360 378 Fragments arranged by size: 966 606 378 360 SanDI GG'GwC_CC Cuts at: 0 885 2310 Size: 885 1425 SexAI A'CCwGG_T Cuts at: 0 1602 2310 Size: 1602 708 SfcI C'TryA_G Cuts at: 0 19 63 346 353 1214 2151 2310 Size: 19 44 283 7 861 937 159 Fragments arranged by size: 937 861 283 159 44 19 7 SmlI C'TyrA_G Cuts at: 0 283 535 1348 1791 2310 Size: 283 252 813 443 519 Fragments arranged by size: 813 519 443 283 252 SphI G_CATG'C Cuts at: 0 987 2310 Size: 987 1323 StuI AGG'CCT Cuts at: 0 1346 2310 Size: 1346 964 StyI C'CwwG_G Cuts at: 0 106 1341 2033 2310 Size: 106 1235 692 277 Fragments arranged by size: 1235 692 277 106 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1342 1857 2310 Size: 1342 515 453 Fragments arranged by size: 1342 515 453 TatI w'GTAC_w Cuts at: 0 894 1721 2310 Size: 894 827 589 Fragments arranged by size: 894 827 589 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1592 1624 1788 1820 2310 Size: 1592 32 164 32 490 Fragments arranged by size: 1592 490 164 32 32 XbaI T'CTAG_A Cuts at: 0 471 2310 Size: 471 1839 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 402 1496 2310 Size: 402 1094 814 Fragments arranged by size: 1094 814 402 Enzymes that do cut: Acc65I AcuI AfeI AflIII AleI AlfI AlwNI ApoI BamHI BanI BbsI BbvCI BclI BfrBI BglI BglII Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI EarI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII KpnI MfeI MmeI MslI MspA1I NdeI NsiI NspI PciI PflMI PfoI PpiI PpuMI PshAI PsrI PstI PvuII SanDI SexAI SfcI SmlI SphI StuI StyI TaqII TatI TstI XbaI XcmI Enzymes that do not cut: AarI AatII AccI AclI AflII AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanII BbeI BcgI BciVI BlpI BmgBI BmrI BmtI BsaBI BsiWI BsmBI BspHI BsrDI BssHII BssSI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI MluI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PasI PmeI PmlI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XhoI XmaI XmnI ZraI