Restriction map for pF1KE4271
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       AspI        AviII       AvrII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BsaHI       
BsiWI       BspDI       BspLU11I    BsrBI       BsrFI       
BssHII      BssSI       BstUI       Bsu36I      Cfr10I      
ClaI        DraII       DrdI        DsaI        Eam1105I    
Eco47III    Eco81I      EcoO109I    FbaI        FseI        
FspI        Hin1I       HindIII     KasI        KpnI        
KspI        MluI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NruI        
NspI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        SacII       SalI        
SapI        SbfI        ScaI        SfoI        SgrAI       
SmaI        SpeI        SphI        Sse8387I    StuI        
SwaI        Tth111I     Van91I      XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccIII       2003  TCCGGA               T^CCGGA
Asp700I       411  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          800  CCCGAG               C^[CT]CG[GA]G
BalI         2510  TGGCCA               TGG^CCA
BamHI        3123  GGATCC               G^GATCC
BanI         2588  GGCACC               G^G[CT][GA]CC
BbsI          518  GTCTTC               GAAGAC(2/6)
BglI          169  GCCGCAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         518  GTCTTC               GAAGAC(2/6)
BsaI          264  GAGACC               GGTCTC(1/5)
BseAI        2003  TCCGGA               T^CCGGA
BsmBI        1716  GAGACG               CGTCTC(1/5)
BsoBI         800  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     2550  TGTACA               T^GTACA
BspEI        2003  TCCGGA               T^CCGGA
BsrDI        2964  CATTGC               GCAATG(2/0)
BsrGI        2550  TGTACA               T^GTACA
BstBI         555  TTCGAA               TT^CGAA
BstEII       1737  GGTGACC              G^GT[ACGT]ACC
BstPI        1737  GGTGACC              G^GT[ACGT]ACC
BstXI        2293  CCAACTGATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI         2005  CGGACCG              CG^G[AT]CCG
EcoO65I      1737  GGTGACC              G^GT[ACGT]ACC
EcoRV         511  GATATC               GAT^ATC
HgaI           19  GCGTC                GACGC(5/10)
HpaI         1477  GTTAAC               GTT^AAC
MfeI         2783  CAATTG               C^AATTG
MluNI        2510  TGGCCA               TGG^CCA
MroI         2003  TCCGGA               T^CCGGA
MscI         2510  TGGCCA               TGG^CCA
MunI         2783  CAATTG               C^AATTG
NspV          555  TTCGAA               TT^CGAA
PvuII         220  CAGCTG               CAG^CTG
RsrII        2005  CGGACCG              CG^G[AT]CCG
SfiI          168  GGCCGCAGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI          555  TTCGAA               TT^CGAA
SnaBI        1844  TACGTA               TAC^GTA
SspBI        2550  TGTACA               T^GTACA
SspI          811  AATATT               AAT^ATT
XbaI         2430  TCTAGA               T^CTAGA
XcmI          135  CCAGGTGGACCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          411  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AlwNI          47  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              155  CAGCGGCTG           
BanII         805  GAGCTC               G[GA]GC[CT]^C
             2706  GAGCTC              
BlpI           44  GCTCAGC              GC^T[ACGT]AGC
             2521  GCTAAGC             
BpmI           39  CTCCAG               CTGGAG(16/14)
             3236  CTGGAG              
Bpu1102I       44  GCTCAGC              GC^T[ACGT]AGC
             2521  GCTAAGC             
BsaAI         232  CACGTA               [CT]AC^GT[GA]
             1844  TACGTA              
BsaBI        1027  GATGTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2733  GATTACCATC          
BsaWI        2003  TCCGGA               [AT]^CCGG[AT]
             2683  ACCGGA              
BsiEI         167  CGGCCG               CG[GA][CT]^CG
              176  CGGCCG              
BsmI          534  GAATGC               GAATGC(1/-1)
             2180  GAATGC              
BspHI         627  TCATGA               T^CATGA
             2949  TCATGA              
BspMI        1968  ACCTGC               ACCTGC(4/8)
             3353  ACCTGC              
Bst1107I      851  GTATAC               GTA^TAC
             2288  GTATAC              
BstYI        2174  AGATCC               [GA]^GATC[CT]
             3123  GGATCC              
BstZ17I       851  GTATAC               GTA^TAC
             2288  GTATAC              
CelII          44  GCTCAGC              GC^T[ACGT]AGC
             2521  GCTAAGC             
DraI         1573  TTTAAA               TTT^AAA
             2107  TTTAAA              
DraIII       2590  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3067  CACCAGGTG           
EagI          167  CGGCCG               C^GGCCG
              176  CGGCCG              
EclXI         167  CGGCCG               C^GGCCG
              176  CGGCCG              
Eco52I        167  CGGCCG               C^GGCCG
              176  CGGCCG              
EcoNI        1183  CCTGCCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2508  CCTGGCCAAGG         
EcoT14I      2513  CCAAGG               C^C[AT][AT]GG
             2909  CCTTGG              
EcoT22I      2182  ATGCAT               ATGCA^T
             3337  ATGCAT              
FauI          205  CCCGC                CCCGC(4/6)
              213  CCCGC               
MamI         1027  GATGTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2733  GATTACCATC          
MflI         2174  AGATCC               [GA]^GATC[CT]
             3123  GGATCC              
NotI          166  GCGGCCGC             GC^GGCCGC
              175  GCGGCCGC            
NsiI         2182  ATGCAT               ATGCA^T
             3337  ATGCAT              
RcaI          627  TCATGA               T^CATGA
             2949  TCATGA              
SacI          805  GAGCTC               GAGCT^C
             2706  GAGCTC              
SexAI         134  ACCAGGT              A^CC[AT]GGT
             3068  ACCAGGT             
SmlI         2980  CTTGAG               C^T[CT][GA]AG
             3310  CTCAAG              
StyI         2513  CCAAGG               C^C[AT][AT]GG
             2909  CCTTGG              
XhoII        2174  AGATCC               [GA]^GATC[CT]
             3123  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AseI        AsnI        AspHI       
AvaII       BbvI        BcnI        BfaI        BmyI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstAPI      BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EaeI        
EarI        EcoRI       EcoRII      Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PshBI       
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
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