Restriction map for pF1KE4271
 (Linear) (Six-base) MAPSORT of: KIEE4271.seq  Check: 2232  from: 1  to: 3411



KIEE4271.orf

 With 182 enzymes: SgfI * 

                        March 15, 2013 17:10  ..

AccI GT'mk_AC

Cuts at:      0      88     852    2289    3411 
   Size:         88     764    1437    1122

  Fragments arranged by size: 

                1437    1122     764      88
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     569    3411 
   Size:        569    2842
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     749    3411 
   Size:        749    2662
 
AlwNI CAG_nnn'CTG

Cuts at:      0      52     160    3411 
   Size:         52     108    3251

  Fragments arranged by size: 

                3251     108      52
 
ApoI r'AATT_y

Cuts at:      0     415     633    1355    1534    1569    1597    1951    2021 
   Size:        415     218     722     179      35      28     354      70

Cuts at:   2021    2153    2437    3242    3411 
   Size:        132     284     805     169

  Fragments arranged by size: 

                 805     722     415     354     284     218     179     169
                 132      70      35      28
 
AseI AT'TA_AT

Cuts at:      0     569    1610    3286    3411 
   Size:        569    1041    1676     125

  Fragments arranged by size: 

                1676    1041     569     125
 
AvaI C'yCGr_G

Cuts at:      0     800    3411 
   Size:        800    2611
 
BamHI G'GATC_C

Cuts at:      0    3123    3411 
   Size:       3123     288
 
BanI G'GyrC_C

Cuts at:      0    2588    3411 
   Size:       2588     823
 
BanII G_rGCy'C

Cuts at:      0     809    2710    3411 
   Size:        809    1901     701

  Fragments arranged by size: 

                1901     809     701
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     511    3411 
   Size:        511    2900
 
BfrBI ATG'CAT

Cuts at:      0    2184    3339    3411 
   Size:       2184    1155      72

  Fragments arranged by size: 

                2184    1155      72
 
BglI GCCn_nnn'nGGC

Cuts at:      0     175    3411 
   Size:        175    3236
 
BlpI GC'TnA_GC

Cuts at:      0      45    2522    3411 
   Size:         45    2477     889

  Fragments arranged by size: 

                2477     889      45
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      24    3257    3411 
   Size:         24    3233     154

  Fragments arranged by size: 

                3233     154      24
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3001    3295    3411 
   Size:       3001     294     116

  Fragments arranged by size: 

                3001     294     116
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     258    3411 
   Size:        258    3153
 
BsaAI yAC'GTr

Cuts at:      0     234    1846    3411 
   Size:        234    1612    1565

  Fragments arranged by size: 

                1612    1565     234
 
BsaBI GATnn'nnATC

Cuts at:      0    1031    2737    3411 
   Size:       1031    1706     674

  Fragments arranged by size: 

                1706    1031     674
 
BsaWI w'CCGG_w

Cuts at:      0    2003    2683    3411 
   Size:       2003     680     728

  Fragments arranged by size: 

                2003     728     680
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1013    1043    3411 
   Size:       1013      30    2368

  Fragments arranged by size: 

                2368    1013      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     102     105    2023    3385    3411 
   Size:        102       3    1918    1362      26

  Fragments arranged by size: 

                1918    1362     102      26       3
 
BseYI C'CCAG_C

Cuts at:      0     189     198     218    1742    2610    3411 
   Size:        189       9      20    1524     868     801

  Fragments arranged by size: 

                1524     868     801     189      20       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146    1198    1363    2207    3411 
   Size:        146    1052     165     844    1204

  Fragments arranged by size: 

                1204    1052     844     165     146
 
BsiEI CG_ry'CG

Cuts at:      0     170     179    3411 
   Size:        170       9    3232

  Fragments arranged by size: 

                3232     170       9
 
BsiHKAI G_wGCw'C

Cuts at:      0     809    2710    2947    3411 
   Size:        809    1901     237     464

  Fragments arranged by size: 

                1901     809     464     237
 
BsmI GAATG_Cn'

Cuts at:      0     540    2186    3411 
   Size:        540    1646    1225

  Fragments arranged by size: 

                1646    1225     540
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1710    3411 
   Size:       1710    1701
 
Bsp1286I G_dGCh'C

Cuts at:      0     809    2710    2947    3411 
   Size:        809    1901     237     464

  Fragments arranged by size: 

                1901     809     464     237
 
BspEI T'CCGG_A

Cuts at:      0    2003    3411 
   Size:       2003    1408
 
BspHI T'CATG_A

Cuts at:      0     627    2949    3411 
   Size:        627    2322     462

  Fragments arranged by size: 

                2322     627     462
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1977    3362    3411 
   Size:       1977    1385      49

  Fragments arranged by size: 

                1977    1385      49
 
BsrDI GCAATG_nn'

Cuts at:      0    2963    3411 
   Size:       2963     448
 
BsrGI T'GTAC_A

Cuts at:      0    2550    3411 
   Size:       2550     861
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     160     535     793    3411 
   Size:        160     375     258    2618

  Fragments arranged by size: 

                2618     375     258     160
 
BstBI TT'CG_AA

Cuts at:      0     556    3411 
   Size:        556    2855
 
BstEII G'GTnAC_C

Cuts at:      0    1737    3411 
   Size:       1737    1674
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2300    3411 
   Size:       2300    1111
 
BstYI r'GATC_y

Cuts at:      0    2174    3123    3411 
   Size:       2174     949     288

  Fragments arranged by size: 

                2174     949     288
 
BstZ17I GTA'TAC

Cuts at:      0     853    2290    3411 
   Size:        853    1437    1121

  Fragments arranged by size: 

                1437    1121     853
 
BtsI GCAGTG_nn'

Cuts at:      0    2415    2477    3411 
   Size:       2415      62     934

  Fragments arranged by size: 

                2415     934      62
 
DraI TTT'AAA

Cuts at:      0    1575    2109    3411 
   Size:       1575     534    1302

  Fragments arranged by size: 

                1575    1302     534
 
DraIII CAC_nnn'GTG

Cuts at:      0    2595    3072    3411 
   Size:       2595     477     339

  Fragments arranged by size: 

                2595     477     339
 
EaeI y'GGCC_r

Cuts at:      0     167     176    2510    3411 
   Size:        167       9    2334     901

  Fragments arranged by size: 

                2334     901     167       9
 
EagI C'GGCC_G

Cuts at:      0     167     176    3411 
   Size:        167       9    3235

  Fragments arranged by size: 

                3235     167       9
 
EarI CTCTTCn'nnn_

Cuts at:      0     256    2928    3407    3411 
   Size:        256    2672     479       4

  Fragments arranged by size: 

                2672     479     256       4
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3023    3411 
   Size:       3023     388
 
EcoICRI GAG'CTC

Cuts at:      0     807    2708    3411 
   Size:        807    1901     703

  Fragments arranged by size: 

                1901     807     703
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      24     569    3257    3411 
   Size:         24     545    2688     154

  Fragments arranged by size: 

                2688     545     154      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1187    2512    3411 
   Size:       1187    1325     899

  Fragments arranged by size: 

                1325    1187     899
 
EcoRI G'AATT_C

Cuts at:      0     415    1355    1534    3411 
   Size:        415     940     179    1877

  Fragments arranged by size: 

                1877     940     415     179
 
EcoRV GAT'ATC

Cuts at:      0     513    3411 
   Size:        513    2898
 
HaeII r_GCGC'y

Cuts at:      0     152     187     196    1315    3411 
   Size:        152      35       9    1119    2096

  Fragments arranged by size: 

                2096    1119     152      35       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     447     479    1774    1806    1821    1853    2931    2963 
   Size:        447      32    1295      32      15      32    1078      32

Cuts at:   2963    2989    3021    3083    3115    3411 
   Size:         26      32      62      32     296

  Fragments arranged by size: 

                1295    1078     447     296      62      32      32      32
                  32      32      32      26      15
 
HincII GTy'rAC

Cuts at:      0    1443    1479    2493    3411 
   Size:       1443      36    1014     918

  Fragments arranged by size: 

                1443    1014     918      36
 
HpaI GTT'AAC

Cuts at:      0    1479    3411 
   Size:       1479    1932
 
MfeI C'AATT_G

Cuts at:      0    2783    3411 
   Size:       2783     628
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     336    2292    3411 
   Size:        336    1956    1119

  Fragments arranged by size: 

                1956    1119     336
 
MscI TGG'CCA

Cuts at:      0    2512    3411 
   Size:       2512     899
 
MslI CAynn'nnrTG

Cuts at:      0     712     731    2812    3411 
   Size:        712      19    2081     599

  Fragments arranged by size: 

                2081     712     599      19
 
MspA1I CmG'CkG

Cuts at:      0     157     166     175     222    3411 
   Size:        157       9       9      47    3189

  Fragments arranged by size: 

                3189     157      47       9       9
 
NotI GC'GGCC_GC

Cuts at:      0     167     176    3411 
   Size:        167       9    3235

  Fragments arranged by size: 

                3235     167       9
 
NsiI A_TGCA'T

Cuts at:      0    2186    3341    3411 
   Size:       2186    1155      70

  Fragments arranged by size: 

                2186    1155      70
 
PfoI T'CCnGG_A

Cuts at:      0     979    1214    2384    3234    3411 
   Size:        979     235    1170     850     177

  Fragments arranged by size: 

                1170     979     850     235     177
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1781    1813    3411 
   Size:       1781      32    1598

  Fragments arranged by size: 

                1781    1598      32
 
PstI C_TGCA'G

Cuts at:      0     156     165     470     939    1265    2410    2542    2772 
   Size:        156       9     305     469     326    1145     132     230

Cuts at:   2772    2826    3359    3411 
   Size:         54     533      52

  Fragments arranged by size: 

                1145     533     469     326     305     230     156     132
                  54      52       9
 
PvuII CAG'CTG

Cuts at:      0     222    3411 
   Size:        222    3189
 
RsrII CG'GwC_CG

Cuts at:      0    2006    3411 
   Size:       2006    1405
 
SacI G_AGCT'C

Cuts at:      0     809    2710    3411 
   Size:        809    1901     701

  Fragments arranged by size: 

                1901     809     701
 
SexAI A'CCwGG_T

Cuts at:      0     134    3068    3411 
   Size:        134    2934     343

  Fragments arranged by size: 

                2934     343     134
 
SfcI C'TryA_G

Cuts at:      0     152     161     239     466     935    1261    1642    1875 
   Size:        152       9      78     227     469     326     381     233

Cuts at:   1875    2406    2538    2768    2822    3355    3411 
   Size:        531     132     230      54     533      56

  Fragments arranged by size: 

                 533     531     469     381     326     233     230     227
                 152     132      78      56      54       9
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     175    3411 
   Size:        175    3236
 
SmlI C'TyrA_G

Cuts at:      0    2980    3310    3411 
   Size:       2980     330     101

  Fragments arranged by size: 

                2980     330     101
 
SnaBI TAC'GTA

Cuts at:      0    1846    3411 
   Size:       1846    1565
 
SspI AAT'ATT

Cuts at:      0     813    3411 
   Size:        813    2598
 
StyI C'CwwG_G

Cuts at:      0    2513    2909    3411 
   Size:       2513     396     502

  Fragments arranged by size: 

                2513     502     396
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1725    2686    2806    3040    3411 
   Size:       1725     961     120     234     371

  Fragments arranged by size: 

                1725     961     371     234     120
 
TatI w'GTAC_w

Cuts at:      0     496    2550    2848    3411 
   Size:        496    2054     298     563

  Fragments arranged by size: 

                2054     563     496     298
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     893     925    1013    1045    3015    3047    3197    3229 
   Size:        893      32      88      32    1970      32     150      32

Cuts at:   3229    3411 
   Size:        182

  Fragments arranged by size: 

                1970     893     182     150      88      32      32      32
                  32
 
XbaI T'CTAG_A

Cuts at:      0    2430    3411 
   Size:       2430     981
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     142    3411 
   Size:        142    3269
 
XmnI GAAnn'nnTTC

Cuts at:      0     415    3411 
   Size:        415    2996
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    AlwNI     ApoI     AseI     AvaI    BamHI
     BanI    BanII     BbsI    BfrBI     BglI     BlpI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrGI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
     BtsI     DraI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI    EcoRI    EcoRV    HaeII    Hin4I   HincII     HpaI
     MfeI     MmeI     MscI     MslI   MspA1I     NotI     NsiI     PfoI
     PsrI     PstI    PvuII    RsrII     SacI    SexAI     SfcI     SfiI
     SmlI    SnaBI     SspI     StyI    TaqII     TatI     TstI     XbaI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII
     BaeI     BbeI    BbvCI     BcgI    BciVI     BclI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BsaHI    BsiWI    BsrBI
    BsrFI   BssHII    BssSI   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DrdI EcoO109I     FalI     FseI     FspI    FspAI  HindIII     KasI
     KpnI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NruI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfoI     SgfI    SgrAI
     SmaI     SpeI     SphI     SrfI     StuI     SwaI  Tth111I     XhoI
     XmaI     ZraI
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