Restriction map for pF1KE9645
##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
Aor51HI     ApaI        AscI        Asp718I     AviII       
AvrII       BalI        BanI        BbeI        BcgI        
BcnI        BglI        BlnI        BsaHI       BseAI       
BsiWI       BspDI       BspEI       BsrBI       BssHII      
BssSI       Bst1107I    BstZ17I     CfoI        ClaI        
CpoI        DrdI        EaeI        EagI        EclXI       
Eco47III    Eco52I      EcoRV       FseI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HpaI        
KasI        KpnI        KspI        MluI        MluNI       
MroI        MscI        NaeI        NarI        NciI        
NgoMIV      NotI        NruI        PacI        PaeR7I      
PmeI        PshAI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    StuI        SwaI        XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI         7124  GTAGAC               GT^[AC][GT]AC
AccII         478  CGCG                 CG^CG
AgeI         1465  ACCGGT               A^CCGGT
AhdI          524  GACAACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       7421  GTGCAC               G^TGCAC
ApaLI        7421  GTGCAC               G^TGCAC
AspEI         524  GACAACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          823  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         5885  CTCGGG               C^[CT]CG[GA]G
BamHI        5311  GGATCC               G^GATCC
BbrPI        1970  CACGTG               CAC^GTG
BglII        5881  AGATCT               A^GATCT
BlpI            7  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I        7  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1970  CACGTG               [CT]AC^GT[GA]
BsaI         1081  GAGACC               GGTCTC(1/5)
BsaWI        1465  ACCGGT               [AT]^CCGG[AT]
BsiEI        1996  CGACCG               CG[GA][CT]^CG
BsmBI        4506  GAGACG               CGTCTC(1/5)
BsoBI        5885  CTCGGG               C^[CT]CG[GA]G
Bsp1407I      381  TGTACA               T^GTACA
BspLU11I     2857  ACATGT               A^CATGT
BsrFI        1465  ACCGGT               [GA]^CCGG[CT]
BsrGI         381  TGTACA               T^GTACA
BstEII       7300  GGTCACC              G^GT[ACGT]ACC
BstPI        7300  GGTCACC              G^GT[ACGT]ACC
BstUI         478  CGCG                 CG^CG
BstXI         761  CCACAATAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII           7  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1465  ACCGGT               [GA]^CCGG[CT]
DraIII       4584  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      524  GACAACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      7300  GGTCACC              G^GT[ACGT]ACC
FauI         2311  GCGGG                CCCGC(4/6)
HapII        1466  CCGG                 C^CGG
HpaII        1466  CCGG                 C^CGG
MfeI          251  CAATTG               C^AATTG
MspI         1466  CCGG                 C^CGG
MunI          251  CAATTG               C^AATTG
MvnI          478  CGCG                 CG^CG
NdeI         3257  CATATG               CA^TATG
NheI         2202  GCTAGC               G^CTAGC
PflFI         823  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1465  ACCGGT               A^CCGGT
PmaCI        1970  CACGTG               CAC^GTG
PmlI         1970  CACGTG               CAC^GTG
PstI         5005  CTGCAG               CTGCA^G
SapI          197  GCTCTTC              GCTCTTC(1/4)
ScaI         5543  AGTACT               AGT^ACT
SpeI          958  ACTAGT               A^CTAGT
SspBI         381  TGTACA               T^GTACA
Tth111I       823  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AflII        1174  CTTAAG               C^TTAAG
             4489  CTTAAG              
AflIII       1971  ACGTGT               A^C[GA][CT]GT
             2857  ACATGT              
AocI         3085  CCTAAGG              CC^T[ACGT]AGG
             4984  CCTCAGG             
BbvCI        3472  GCTGAGG              CCTCAGC(-5/-2)
             6031  GCTGAGG             
BfrI         1174  CTTAAG               C^TTAAG
             4489  CTTAAG              
BsaBI        3628  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             7197  GATGTTAATC          
BstAPI        354  GCAGAGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              719  GCAAGAAATGC         
Bsu36I       3085  CCTAAGG              CC^T[ACGT]AGG
             4984  CCTCAGG             
DsaI         5378  CCATGG               C^C[GA][CT]GG
             6509  CCATGG              
Eco81I       3085  CCTAAGG              CC^T[ACGT]AGG
             4984  CCTCAGG             
MamI         3628  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             7197  GATGTTAATC          
NcoI         5378  CCATGG               C^CATGG
             6509  CCATGG              
PflMI        4779  CCACTGTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             7384  CCAGTGGCTGG         
PpuMI        6974  AGGACCT              [GA]G^G[AT]CC[CT]
             7452  AGGACCT             
SacI         2397  GAGCTC               GAGCT^C
             6715  GAGCTC              
Van91I       4779  CCACTGTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             7384  CCAGTGGCTGG         
XcmI         4686  CCATCAAGTAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5989  CCAGCTCCAGACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AseI        AsnI        Asp700I     
AspHI       AvaII       BanII       BbsI        BbvI        
BciVI       BclI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    BspHI       BspMI       BsrDI       BsrI        
BssKI       BstBI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraI        
DraII       EarI        EcoNI       EcoO109I    EcoRI       
EcoRII      EcoT14I     EcoT22I     FbaI        Fnu4HI      
FokI        HaeIII      HgaI        HincII      HindII      
HindIII     HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NsiI        NspI        NspV        PleI        PshBI       
PvuII       RcaI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfuI        SmlI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI        
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