Restriction map for pF1KE9645
 (Circular) (Six-base) MAPSORT of: pF1KE9645.seq  Check: 9397  from: 1  to: 10589



pF1KE9645  10589 bp

 With 182 enzymes: SgfI * 

                        May 29, 2012 11:58  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   5701    6678    5701 
   Size:        977    9612
 
AccI GT'mk_AC

Cuts at:   7233    7627    7233 
   Size:        394   10195
 
Acc65I G'GTAC_C

Cuts at:   7605    7605 
   Size:      10589
 
AclI AA'CG_TT

Cuts at:  10429   10429 
   Size:      10589
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    215     293     311    1658    2339    2678    2759    3062    3077 
   Size:         78      18    1347     681     339      81     303      15

Cuts at:   3077    3095    3320    3614    3824    3965    4112    6425    6814 
   Size:         18     225     294     210     141     147    2313     389

Cuts at:   6814    6929    8414    8846    9593    9861     215 
   Size:        115    1485     432     747     268     943

  Fragments arranged by size: 

                2313    1485    1347     943     747     681     432     389
                 339     303     294     268     225     210     147     141
                 115      81      78      18      18      15
 
AflII C'TTAA_G

Cuts at:   1282    4597    1282 
   Size:       3315    7274
 
AflIII A'CryG_T

Cuts at:   2079    2965    7941    9045   10022    2079 
   Size:        886    4976    1104     977    2646

  Fragments arranged by size: 

                4976    2646    1104     977     886
 
AgeI A'CCGG_T

Cuts at:   1573    8977    1573 
   Size:       7404    3185
 
AhdI GACnn_n'nnGTC

Cuts at:    637     637 
   Size:      10589
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2867    2867 
   Size:      10589
 
AlwNI CAG_nnn'CTG

Cuts at:   2278    2299    2362    3871    4090    5245    5851    6133    6660 
   Size:         21      63    1509     219    1155     606     282     527

Cuts at:   6660    7498    7894    9461    2278 
   Size:        838     396    1567    3406

  Fragments arranged by size: 

                3406    1567    1509    1155     838     606     527     396
                 282     219      63      21
 
ApaLI G'TGCA_C

Cuts at:   7529    9359    7529 
   Size:       1830    8759
 
ApoI r'AATT_y

Cuts at:    438     541     963    1222    1347    2289    2375    2627    2706 
   Size:        103     422     259     125     942      86     252      79

Cuts at:   2706    3007    3179    3522    3747    3801    4544    5017    5313 
   Size:        301     172     343     225      54     743     473     296

Cuts at:   5313    5409    5581    5833    5982    6182    6409    7434    7593 
   Size:         96     172     252     149     200     227    1025     159

Cuts at:   7593    9942   10198     438 
   Size:       2349     256     829

  Fragments arranged by size: 

                2349    1025     942     829     743     473     422     343
                 301     296     259     256     252     252     227     225
                 200     172     172     159     149     125     103      96
                  86      79      54
 
AseI AT'TA_AT

Cuts at:     20    2369    6479    7091      20 
   Size:       2349    4110     612    3518

  Fragments arranged by size: 

                4110    3518    2349     612
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:      10589
 
AvaI C'yCGr_G

Cuts at:   5993    7609    5993 
   Size:       1616    8973
 
BamHI G'GATC_C

Cuts at:   5419    7614    5419 
   Size:       2195    8394
 
BanI G'GyrC_C

Cuts at:   7605    8019    8267    8302    7605 
   Size:        414     248      35    9892

  Fragments arranged by size: 

                9892     414     248      35
 
BanII G_rGCy'C

Cuts at:    122    2509    6827    7053    7603    8633     122 
   Size:       2387    4318     226     550    1030    2078

  Fragments arranged by size: 

                4318    2387    2078    1030     550     226
 
BbeI G_GCGC'C

Cuts at:   8023    8271    8023 
   Size:        248   10341
 
BbsI GAAGACnn'nnnn_

Cuts at:    317     521    1069    1346    1691    2540    2719    3806    5825 
   Size:        204     548     277     345     849     179    1087    2019

Cuts at:   5825    6295     317 
   Size:        470    4611

  Fragments arranged by size: 

                4611    2019    1087     849     548     470     345     277
                 204     179
 
BbvCI CC'TCA_GC

Cuts at:   3581    6140    3581 
   Size:       2559    8030
 
BciVI GTATCCnnnnn_n'

Cuts at:   6780    6801    6850    8487    9248   10589    6780 
   Size:         21      49    1637     761    1341    6780

  Fragments arranged by size: 

                6780    1637    1341     761      49      21
 
BclI T'GATC_A

Cuts at:   1734    3741    5144    6678    7439    8109    1734 
   Size:       2007    1403    1534     761     670    4214

  Fragments arranged by size: 

                4214    2007    1534    1403     761     670
 
BfrBI ATG'CAT

Cuts at:    836    1530    4877     836 
   Size:        694    3347    6548

  Fragments arranged by size: 

                6548    3347     694
 
BglII A'GATC_T

Cuts at:   5989    8104   10141    5989 
   Size:       2115    2037    6437

  Fragments arranged by size: 

                6437    2115    2037
 
BlpI GC'TnA_GC

Cuts at:    116    7701     116 
   Size:       7585    3004
 
Bme1580I G_kGCm'C

Cuts at:   1154    2702    7533    8214    8307    9363    1154 
   Size:       1548    4831     681      93    1056    2380

  Fragments arranged by size: 

                4831    2380    1548    1056     681      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   2532    6995    7414    7801    7882    8217   10361    2532 
   Size:       4463     419     387      81     335    2144    2760

  Fragments arranged by size: 

                4463    2760    2144     419     387     335      81
 
BmtI G_CTAG'C

Cuts at:   2314    2314 
   Size:      10589
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2787    6806    8956    2787 
   Size:       4019    2150    4420

  Fragments arranged by size: 

                4420    4019    2150
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1194    4184    4847    6086    7099    7333    1194 
   Size:       2990     663    1239    1013     234    4450

  Fragments arranged by size: 

                4450    2990    1239    1013     663     234
 
Bpu10I CC'TnA_GC

Cuts at:   2240    3167    3581    6140    6608    2240 
   Size:        927     414    2559     468    6221

  Fragments arranged by size: 

                6221    2559     927     468     414
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1723    2118    5080    5162    5919    7177    7765    8644    9136 
   Size:        395    2962      82     757    1258     588     879     492

Cuts at:   9136    9434    1723 
   Size:        298    2878

  Fragments arranged by size: 

                2962    2878    1258     879     757     588     492     395
                 298      82
 
BsaI GGTCTCn'nnnn_

Cuts at:   1183   10293    1183 
   Size:       9110    1479
 
BsaAI yAC'GTr

Cuts at:   2080    7942    2080 
   Size:       5862    4727
 
BsaBI GATnn'nnATC

Cuts at:      6    3740    7309    8103   10140       6 
   Size:       3734    3569     794    2037     455

  Fragments arranged by size: 

                3734    3569    2037     794     455
 
BsaHI Gr'CG_yC

Cuts at:   8020    8268    8970   10466    8020 
   Size:        248     702    1496    8143

  Fragments arranged by size: 

                8143    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1573    7778    8299    8977    9251    9398    9809    1573 
   Size:       6205     521     678     274     147     411    2353

  Fragments arranged by size: 

                6205    2353     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    192     222    1192    1222     192 
   Size:         30     970      30    9559

  Fragments arranged by size: 

                9559     970      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    200    1835    2905    4459    4474    4477    4486    4489    4492 
   Size:       1635    1070    1554      15       3       9       3       3

Cuts at:   4492    4495    4504    4507    5346     200 
   Size:          3       9       3     839    5443

  Fragments arranged by size: 

                5443    1635    1554    1070     839      15       9       9
                   3       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:   6096    8014    8772    9349    9845    6096 
   Size:       1918     758     577     496    6840

  Fragments arranged by size: 

                6840    1918     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    172     603    4777    5495    6365     172 
   Size:        431    4174     718     870    4396

  Fragments arranged by size: 

                4396    4174     870     718     431
 
BsiEI CG_ry'CG

Cuts at:    104    2107    8131    8177    9385     104 
   Size:       2003    6024      46    1208    1308

  Fragments arranged by size: 

                6024    2003    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1526    2509    5520    6827    7533    7603    8381    8571    9363 
   Size:        983    3011    1307     706      70     778     190     792

Cuts at:   9363    1526 
   Size:       2752

  Fragments arranged by size: 

                3011    2752    1307     983     792     778     706     190
                  70
 
BsmI GAATG_Cn'

Cuts at:    836    4877    6153     836 
   Size:       4041    1276    5272

  Fragments arranged by size: 

                5272    4041    1276
 
BsmBI CGTCTCn'nnnn_

Cuts at:   4608    4608 
   Size:      10589
 
Bsp1286I G_dGCh'C

Cuts at:    122    1154    1526    2509    2702    5520    6827    7053    7533 
   Size:       1032     372     983     193    2818    1307     226     480

Cuts at:   7533    7603    8214    8307    8381    8571    8633    9363     122 
   Size:         70     611      93      74     190      62     730    1348

  Fragments arranged by size: 

                2818    1348    1307    1032     983     730     611     480
                 372     226     193     190      93      74      70      62
            
 
BspEI T'CCGG_A

Cuts at:   9809    9809 
   Size:      10589
 
BspHI T'CATG_A

Cuts at:    162    7415    7442    9765     162 
   Size:       7253      27    2323     986

  Fragments arranged by size: 

                7253    2323     986      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1632    4749    5701    6678    7639    7853    8155    8536    1632 
   Size:       3117     952     977     961     214     302     381    3685

  Fragments arranged by size: 

                3685    3117     977     961     952     381     302     214
            
 
BsrBI CCG'CTC

Cuts at:     50    8881    8935   10419   10584      50 
   Size:       8831      54    1484     165      55

  Fragments arranged by size: 

                8831    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    230     679    7067    8501     230 
   Size:        449    6388    1434    2318

  Fragments arranged by size: 

                6388    2318    1434     449
 
BsrFI r'CCGG_y

Cuts at:   1573    8587    8768    8977    1573 
   Size:       7014     181     209    3185

  Fragments arranged by size: 

                7014    3185     209     181
 
BsrGI T'GTAC_A

Cuts at:    489     489 
   Size:      10589
 
BssSI C'ACGA_G

Cuts at:   8860    9218    8860 
   Size:        358   10231
 
BstAPI GCAn_nnn'nTGC

Cuts at:    468     833    7966     468 
   Size:        365    7133    3091

  Fragments arranged by size: 

                7133    3091     365
 
BstBI TT'CG_AA

Cuts at:   2067    6189    6342    8950    2067 
   Size:       4122     153    2608    3706

  Fragments arranged by size: 

                4122    3706    2608     153
 
BstEII G'GTnAC_C

Cuts at:   7408    7408 
   Size:      10589
 
BstXI CCAn_nnnn'nTGG

Cuts at:    876     876 
   Size:      10589
 
BstYI r'GATC_y

Cuts at:      1    2071    3976    5419    5989    6466    7614    8081    8104 
   Size:       2070    1905    1443     570     477    1148     467      23

Cuts at:   8104    8439    8685    9697    9783    9795   10141       1 
   Size:        335     246    1012      86      12     346     449

  Fragments arranged by size: 

                2070    1905    1443    1148    1012     570     477     467
                 449     346     335     246      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   3194    5093    3194 
   Size:       1899    8690
 
BtgI C'CryG_G

Cuts at:   5486    6617    5486 
   Size:       1131    9458
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1378    3044    3785    8539    8720    8883    1378 
   Size:       1666     741    4754     181     163    3084

  Fragments arranged by size: 

                4754    3084    1666     741     181     163
 
BtsI GCAGTG_nn'

Cuts at:    620     938    1761    4239    5955    7145    7832     620 
   Size:        318     823    2478    1716    1190     687    3377

  Fragments arranged by size: 

                3377    2478    1716    1190     823     687     318
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    751     786     751 
   Size:         35   10554
 
DraI TTT'AAA

Cuts at:    531    1229    2408    6050    7588     531 
   Size:        698    1179    3642    1538    3532

  Fragments arranged by size: 

                3642    3532    1538    1179     698
 
DraIII CAC_nnn'GTG

Cuts at:   4697    4697 
   Size:      10589
 
DrdI GACnn_nn'nnGTC

Cuts at:   8295    9153    8295 
   Size:        858    9731
 
EaeI y'GGCC_r

Cuts at:   8174    8348    8739    8766   10111    8174 
   Size:        174     391      27    1345    8652

  Fragments arranged by size: 

                8652    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   8174    8174 
   Size:      10589
 
EarI CTCTTCn'nnn_

Cuts at:    287     312    2658    2778    3339    3409    3483    4436    4451 
   Size:         25    2346     120     561      70      74     953      15

Cuts at:   4451    4463    4481    4598    4604    5188    5694    6775    6893 
   Size:         12      18     117       6     584     506    1081     118

Cuts at:   6893    7095    7260    8612    8822     287 
   Size:        202     165    1352     210    2054

  Fragments arranged by size: 

                2346    2054    1352    1081     953     584     561     506
                 210     202     165     120     118     117      74      70
                  25      18      15      12       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   8797    9107    9253   10399    8797 
   Size:        310     146    1146    8987

  Fragments arranged by size: 

                8987    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   2507    6825    7601    2507 
   Size:       4318     776    5495

  Fragments arranged by size: 

                5495    4318     776
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    215     293     311    1194    1658    2339    2678    2759    3062 
   Size:         78      18     883     464     681     339      81     303

Cuts at:   3062    3077    3095    3320    3614    3824    3965    4112    4184 
   Size:         15      18     225     294     210     141     147      72

Cuts at:   4184    4847    6086    6425    6814    6929    7099    7333    8414 
   Size:        663    1239     339     389     115     170     234    1081

Cuts at:   8414    8846    9593    9861     215 
   Size:        432     747     268     943

  Fragments arranged by size: 

                1239    1081     943     883     747     681     663     464
                 432     389     339     339     303     294     268     234
                 225     210     170     147     141     115      81      78
                  72      18      18      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2323    4529    7568    2323 
   Size:       2206    3039    5344

  Fragments arranged by size: 

                5344    3039    2206
 
EcoO109I rG'GnC_Cy

Cuts at:    535    5242    7083    7561    7728     535 
   Size:       4707    1841     478     167    3396

  Fragments arranged by size: 

                4707    3396    1841     478     167
 
EcoRI G'AATT_C

Cuts at:   2627    3747    5313    5982    7593    2627 
   Size:       1120    1566     669    1611    5623

  Fragments arranged by size: 

                5623    1611    1566    1120     669
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    616     723    1513    3973    6283    8608     616 
   Size:        107     790    2460    2310    2325    2597

  Fragments arranged by size: 

                2597    2460    2325    2310     790     107
 
FspI TGC'GCA

Cuts at:   8370    8370 
   Size:      10589
 
HaeII r_GCGC'y

Cuts at:   8023    8271    9293   10272    8023 
   Size:        248    1022     979    8340

  Fragments arranged by size: 

                8340    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    623     655    1645    1677    1768    1800    2268    2300    2635 
   Size:         32     990      32      91      32     468      32     335

Cuts at:   2635    2667    2934    2966    2974    3006    3271    3303    3415 
   Size:         32     267      32       8      32     265      32     112

Cuts at:   3415    3447    3694    3726    3961    3993    4144    4176    4504 
   Size:         32     247      32     235      32     151      32     328

Cuts at:   4504    4536    5845    5877    5917    5949    6431    6463    6621 
   Size:         32    1309      32      40      32     482      32     158

Cuts at:   6621    6653    7137    7169    7259    7291    7431    7463    8431 
   Size:         32     484      32      90      32     140      32     968

Cuts at:   8431    8461    8463    8493     623 
   Size:         30       2      30    2719

  Fragments arranged by size: 

                2719    1309     990     968     484     482     468     335
                 328     267     265     247     235     158     151     140
                 112      91      90      40      32      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      32      32      32
                  30      30       8       2
 
HincII GTy'rAC

Cuts at:   1008    3308    6905    7628    1008 
   Size:       2300    3597     723    3969

  Fragments arranged by size: 

                3969    3597    2300     723
 
HindIII A'AGCT_T

Cuts at:    157    2875    5056    5225    5719     157 
   Size:       2718    2181     169     494    5027

  Fragments arranged by size: 

                5027    2718    2181     494     169
 
KasI G'GCGC_C

Cuts at:   8019    8267    8019 
   Size:        248   10341
 
KpnI G_GTAC'C

Cuts at:   7609    7609 
   Size:      10589
 
MfeI C'AATT_G

Cuts at:    359     359 
   Size:      10589
 
MluI A'CGCG_T

Cuts at:  10022   10022 
   Size:      10589
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    908    1567    4322    5179    9260    9444     908 
   Size:        659    2755     857    4081     184    2053

  Fragments arranged by size: 

                4081    2755    2053     857     659     184
 
MscI TGG'CCA

Cuts at:   8350    8350 
   Size:      10589
 
MslI CAynn'nnrTG

Cuts at:    874    1535    5603    6866    8578    8705     874 
   Size:        661    4068    1263    1712     127    2758

  Fragments arranged by size: 

                4068    2758    1712    1263     661     127
 
MspA1I CmG'CkG

Cuts at:    115    4308    4356    7077    7174    7316    7700    8014    8374 
   Size:       4193      48    2721      97     142     384     314     360

Cuts at:   8374    9387    9632     115 
   Size:       1013     245    1072

  Fragments arranged by size: 

                4193    2721    1072    1013     384     360     314     245
                 142      97      48
 
NaeI GCC'GGC

Cuts at:   8770    8770 
   Size:      10589
 
NarI GG'CG_CC

Cuts at:   8020    8268    8020 
   Size:        248   10341
 
NcoI C'CATG_G

Cuts at:   5486    6617    5486 
   Size:       1131    9458
 
NdeI CA'TA_TG

Cuts at:   3366    3366 
   Size:      10589
 
NgoMIV G'CCGG_C

Cuts at:   8768    8768 
   Size:      10589
 
NheI G'CTAG_C

Cuts at:   2310    2310 
   Size:      10589
 
NsiI A_TGCA'T

Cuts at:    838    1532    4879     838 
   Size:        694    3347    6548

  Fragments arranged by size: 

                6548    3347     694
 
NspI r_CATG'y

Cuts at:    947    1530    2969    5112    7642    8142    9049     947 
   Size:        583    1439    2143    2530     500     907    2487

  Fragments arranged by size: 

                2530    2487    2143    1439     907     583     500
 
PciI A'CATG_T

Cuts at:   2965    9045    2965 
   Size:       6080    4509
 
PflMI CCAn_nnn'nTGG

Cuts at:   4893    7498    4893 
   Size:       2605    7984
 
PfoI T'CCnGG_A

Cuts at:   4524    7557    4524 
   Size:       3033    7556
 
PmeI GTTT'AAAC

Cuts at:   7588    7588 
   Size:      10589
 
PmlI CAC'GTG

Cuts at:   2080    2080 
   Size:      10589
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2331    2363    9728    9760    2331 
   Size:         32    7365      32    3160

  Fragments arranged by size: 

                7365    3160      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   7083    7561    7083 
   Size:        478   10111
 
PsiI TTA'TAA

Cuts at:   5449    6320    5449 
   Size:        871    9718
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   4793    4825    4793 
   Size:         32   10557
 
PstI C_TGCA'G

Cuts at:   5117    7636    8321    5117 
   Size:       2519     685    7385

  Fragments arranged by size: 

                7385    2519     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:      10589
 
PvuII CAG'CTG

Cuts at:   7077    7174    7316    8014    8374    7077 
   Size:         97     142     698     360    9292

  Fragments arranged by size: 

                9292     698     360     142      97
 
RsrII CG'GwC_CG

Cuts at:   8784    8784 
   Size:      10589
 
SacI G_AGCT'C

Cuts at:   2509    6827    7603    2509 
   Size:       4318     776    5495

  Fragments arranged by size: 

                5495    4318     776
 
SalI G'TCGA_C

Cuts at:   7626    7626 
   Size:      10589
 
SapI GCTCTTCn'nnn_

Cuts at:    312    8612    8822     312 
   Size:       8300     210    2079

  Fragments arranged by size: 

                8300    2079     210
 
SbfI CC_TGCA'GG

Cuts at:   7636    7636 
   Size:      10589
 
ScaI AGT'ACT

Cuts at:   5653   10106    5653 
   Size:       4453    6136
 
SfcI C'TryA_G

Cuts at:     33    1841    2228    3752    5113    7632    8317    9310    9501 
   Size:       1808     387    1524    1361    2519     685     993     191

Cuts at:   9501      33 
   Size:       1121

  Fragments arranged by size: 

                2519    1808    1524    1361    1121     993     685     387
                 191
 
SfoI GGC'GCC

Cuts at:   8021    8269    8021 
   Size:        248   10341
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:      10589
 
SmaI CCC'GGG

Cuts at:   7611    7611 
   Size:      10589
 
SmlI C'TyrA_G

Cuts at:   1282    1702    2097    4597    5059    5177    5934    7156    7744 
   Size:        420     395    2500     462     118     757    1222     588

Cuts at:   7744    8659    9151    9413    1282 
   Size:        915     492     262    2458

  Fragments arranged by size: 

                2500    2458    1222     915     757     588     492     462
                 420     395     262     118
 
SpeI A'CTAG_T

Cuts at:     67    1066      67 
   Size:        999    9590
 
SphI G_CATG'C

Cuts at:   7642    8142    7642 
   Size:        500   10089
 
SspI AAT'ATT

Cuts at:   1800    3451    5216    1800 
   Size:       1651    1765    7173

  Fragments arranged by size: 

                7173    1765    1651
 
StyI C'CwwG_G

Cuts at:    849    4899    5486    5764    6617    7569    7723    8077     849 
   Size:       4050     587     278     853     952     154     354    3361

  Fragments arranged by size: 

                4050    3361     952     853     587     354     278     154
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    929    2121    4704    8172    8305    8764    8973     929 
   Size:       1192    2583    3468     133     459     209    2545

  Fragments arranged by size: 

                3468    2583    2545    1192     459     209     133
 
TatI w'GTAC_w

Cuts at:    489    2976    5651    7212    7358   10104     489 
   Size:       2487    2675    1561     146    2746     974

  Fragments arranged by size: 

                2746    2675    2487    1561     974     146
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4683    4715    5174    5206   10106   10138    4683 
   Size:         32     459      32    4900      32    5134

  Fragments arranged by size: 

                5134    4900     459      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    934    8386     934 
   Size:       7452    3137
 
XbaI T'CTAG_A

Cuts at:   7620    7620 
   Size:      10589
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   4801    6104    8084    4801 
   Size:       1303    1980    7306

  Fragments arranged by size: 

                7306    1980    1303
 
XmaI C'CCGG_G

Cuts at:   7609    7609 
   Size:      10589
 
XmnI GAAnn'nnTTC

Cuts at:   2454    3405    5625    6185    6263    2454 
   Size:        951    2220     560      78    6780

  Fragments arranged by size: 

                6780    2220     951     560      78
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI
    BfrBI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NsiI     NspI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI     PsiI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI     AleI     AloI     ApaI     AscI    AvrII     BaeI
     BcgI     BglI    BmgBI    BsiWI   BssHII  BstZ17I     ClaI    EcoRV
     FseI    FspAI     HpaI     NotI     NruI     PacI     PasI    PshAI
   PspOMI    PspXI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     StuI     SwaI     XhoI     ZraI
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