Restriction map for pF1KB8470
##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AccIII      AclI        AhdI        ApaI        AscI        
AseI        AsnI        AspEI       AviII       AvrII       
BamHI       BbeI        BcgI        BciVI       BclI        
BglI        BlnI        BlpI        Bpu1102I    BsaBI       
BsaI        BseAI       BseRI       BsiWI       BsmAI       
BsmBI       BspDI       BspEI       BspHI       BsrBI       
BssHII      BssSI       Bst1107I    BstBI       BstZ17I     
CelII       ClaI        CpoI        DraI        EagI        
Eam1105I    EarI        EclXI       Eco52I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HpaI        KasI        Ksp632I     MamI        MluI        
MroI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SapI        
SbfI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    StuI        
SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

Acc65I       2261  GGTACC               G^GTACC
AgeI         1276  ACCGGT               A^CCGGT
Alw44I       2017  GTGCAC               G^TGCAC
AocI         1884  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       939  AGCGCT               AGC^GCT
ApaLI        2017  GTGCAC               G^TGCAC
Asp700I       396  GAAACATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2261  GGTACC               G^GTACC
AspI         1944  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         2093  CCCGGG               C^[CT]CG[GA]G
BanI         2261  GGTACC               G^G[CT][GA]CC
BbrPI        2328  CACGTG               CAC^GTG
BbsI         1713  GAAGAC               GAAGAC(2/6)
BbvCI        1918  GCTGAGG              CCTCAGC(-5/-2)
BpuAI        1713  GAAGAC               GAAGAC(2/6)
BsaAI        2328  CACGTG               [CT]AC^GT[GA]
BsgI          132  GTGCAG               GTGCAG(16/14)
BsiEI        1278  CGGTCG               CG[GA][CT]^CG
BsoBI        2093  CCCGGG               C^[CT]CG[GA]G
BspLU11I     1002  ACATGT               A^CATGT
BsrDI        1415  GCAATG               GCAATG(2/0)
BsrFI        1276  ACCGGT               [GA]^CCGG[CT]
BstEII        697  GGTAACC              G^GT[ACGT]ACC
BstPI         697  GGTAACC              G^GT[ACGT]ACC
BstXI        1542  CCAGTACTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1884  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       1276  ACCGGT               [GA]^CCGG[CT]
DraII        1868  GGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII       2011  CACTTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          381  GACGAGTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      939  AGCGCT               AGC^GCT
Eco81I       1884  CCTCAGG              CC^T[ACGT]AGG
EcoNI           8  CCTGGATAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1868  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       697  GGTAACC              G^GT[ACGT]ACC
HaeII         939  AGCGCT               [GA]GCGC^[CT]
HincII        835  GTCAAC               GT[CT]^[GA]AC
HindII        835  GTCAAC               GT[CT]^[GA]AC
HindIII       263  AAGCTT               A^AGCTT
KpnI         2261  GGTACC               GGTAC^C
KspI         1982  CCGCGG               CCGC^GG
NcoI           32  CCATGG               C^CATGG
NdeI          715  CATATG               CA^TATG
PflFI        1944  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2147  CCATTCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1276  ACCGGT               A^CCGGT
PmaCI        2328  CACGTG               CAC^GTG
PmlI         2328  CACGTG               CAC^GTG
PpuMI        1868  GGGACCT              [GA]G^G[AT]CC[CT]
SacI         1768  GAGCTC               GAGCT^C
SacII        1982  CCGCGG               CCGC^GG
ScaI         1544  AGTACT               AGT^ACT
SexAI         701  ACCTGGT              A^CC[AT]GGT
SmaI         2093  CCCGGG               CCC^GGG
TaqI          875  TCGA                 T^CGA
Tth111I      1944  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       875  TCGA                 T^CGA
Van91I       2147  CCATTCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        2093  CCCGGG               C^CCGGG
XmaI         2093  CCCGGG               C^CCGGG
XmnI          396  GAAACATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AatII        1261  GACGTC               GACGT^C
             2203  GACGTC              
AccI         1993  GTCTAC               GT^[AC][GT]AC
             2446  GTCTAC              
AflII         747  CTTAAG               C^TTAAG
             1360  CTTAAG              
AflIII       1002  ACATGT               A^C[GA][CT]GT
             2099  ACGTGT              
AvaII          25  GGACC                G^G[AT]CC
             1869  GGACC               
BcnI         2093  CCCGG                CC^[GC]GG
             2094  CCGGG               
BfrI          747  CTTAAG               C^TTAAG
             1360  CTTAAG              
BglII         788  AGATCT               A^GATCT
             1697  AGATCT              
BsmI         1666  GAATGC               GAATGC(1/-1)
              905  GCATTC              
Bsp1407I     2102  TGTACA               T^GTACA
             2177  TGTACA              
BsrGI        2102  TGTACA               T^GTACA
             2177  TGTACA              
BstAPI       1953  GCAGATGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2010  GCACTTCGTGC         
CfoI           37  GCGC                 GCG^C
              940  GCGC                
EcoT14I        32  CCATGG               C^C[AT][AT]GG
             2432  CCAAGG              
HhaI           37  GCGC                 GCG^C
              940  GCGC                
HinP1I         37  GCGC                 G^CGC
              940  GCGC                
MfeI          290  CAATTG               C^AATTG
              576  CAATTG              
MunI          290  CAATTG               C^AATTG
              576  CAATTG              
NciI         2093  CCCGG                CC^[GC]GG
             2094  CCGGG               
NspI          451  ACATGC               [GA]CATG^[CT]
             1002  ACATGT              
PvuII        1039  CAGCTG               CAG^CTG
             2266  CAGCTG              
SspBI        2102  TGTACA               T^GTACA
             2177  TGTACA              
SspI          547  AATATT               AAT^ATT
              966  AATATT              
StyI           32  CCATGG               C^C[AT][AT]GG
             2432  CCAAGG              
XcmI          937  CCAGCGCTTCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2036  CCAGGAACTGCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
BalI        BanII       BbvI        BfaI        BmyI        
BpmI        BsaHI       BsaJI       BsaWI       BsiHKAI     
BsiYI       BslI        BsmFI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DsaI        EaeI        EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        Hin1I       
HinfI       HpaII       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       PleI        PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XhoII       
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