Restriction map for pF1KB5507
 (Linear) (Six-base) MAPSORT of: KIBB5507.seq  Check: 6694  from: 1  to: 1788



KIBB5507.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     876    1788 
   Size:        271     605     912

  Fragments arranged by size: 

                 912     605     271
 
AflIII A'CryG_T

Cuts at:      0    1454    1788 
   Size:       1454     334
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1174    1788 
   Size:       1174     614
 
AlwNI CAG_nnn'CTG

Cuts at:      0     400    1014    1542    1788 
   Size:        400     614     528     246

  Fragments arranged by size: 

                 614     528     400     246
 
ApaLI G'TGCA_C

Cuts at:      0     458    1788 
   Size:        458    1330
 
ApoI r'AATT_y

Cuts at:      0     306     971    1400    1788 
   Size:        306     665     429     388

  Fragments arranged by size: 

                 665     429     388     306
 
AvaI C'yCGr_G

Cuts at:      0       7     548     880    1788 
   Size:          7     541     332     908

  Fragments arranged by size: 

                 908     541     332       7
 
BamHI G'GATC_C

Cuts at:      0     846    1788 
   Size:        846     942
 
BanII G_rGCy'C

Cuts at:      0       8      68    1788 
   Size:          8      60    1720

  Fragments arranged by size: 

                1720      60       8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1322    1788 
   Size:       1322     466
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1137    1171    1788 
   Size:       1137      34     617

  Fragments arranged by size: 

                1137     617      34
 
BclI T'GATC_A

Cuts at:      0     831    1788 
   Size:        831     957
 
BglII A'GATC_T

Cuts at:      0     906    1788 
   Size:        906     882
 
BlpI GC'TnA_GC

Cuts at:      0      67    1788 
   Size:         67    1721
 
Bme1580I G_kGCm'C

Cuts at:      0     462    1788 
   Size:        462    1326
 
BmgBI CAC'GTC

Cuts at:      0     296    1455    1788 
   Size:        296    1159     333

  Fragments arranged by size: 

                1159     333     296
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     189    1288    1422    1788 
   Size:        189    1099     134     366

  Fragments arranged by size: 

                1099     366     189     134
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     853    1036    1126    1406    1788 
   Size:        853     183      90     280     382

  Fragments arranged by size: 

                 853     382     280     183      90
 
Bpu10I CC'TnA_GC

Cuts at:      0     235    1529    1788 
   Size:        235    1294     259

  Fragments arranged by size: 

                1294     259     235
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1173    1203    1788 
   Size:       1173      30     585

  Fragments arranged by size: 

                1173     585      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1373    1620    1788 
   Size:       1373     247     168

  Fragments arranged by size: 

                1373     247     168
 
BseYI C'CCAG_C

Cuts at:      0     393    1788 
   Size:        393    1395
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1537    1788 
   Size:       1537     251
 
BsiEI CG_ry'CG

Cuts at:      0      37      98    1788 
   Size:         37      61    1690

  Fragments arranged by size: 

                1690      61      37
 
BsiHKAI G_wGCw'C

Cuts at:      0     462     998    1788 
   Size:        462     536     790

  Fragments arranged by size: 

                 790     536     462
 
BsmI GAATG_Cn'

Cuts at:      0     786    1788 
   Size:        786    1002
 
Bsp1286I G_dGCh'C

Cuts at:      0       8      68     462     998    1788 
   Size:          8      60     394     536     790

  Fragments arranged by size: 

                 790     536     394      60       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     410    1328    1788 
   Size:        410     918     460

  Fragments arranged by size: 

                 918     460     410
 
BsrBI CCG'CTC

Cuts at:      0    1492    1788 
   Size:       1492     296
 
BsrDI GCAATG_nn'

Cuts at:      0    1496    1788 
   Size:       1496     292
 
BsrFI r'CCGG_y

Cuts at:      0    1280    1788 
   Size:       1280     508
 
BssSI C'ACGA_G

Cuts at:      0     287     757    1788 
   Size:        287     470    1031

  Fragments arranged by size: 

                1031     470     287
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1542    1788 
   Size:       1542     246
 
BstEII G'GTnAC_C

Cuts at:      0     379     767    1788 
   Size:        379     388    1021

  Fragments arranged by size: 

                1021     388     379
 
BstYI r'GATC_y

Cuts at:      0     388     573     846     906    1788 
   Size:        388     185     273      60     882

  Fragments arranged by size: 

                 882     388     273     185      60
 
BtgI C'CryG_G

Cuts at:      0      37    1167    1788 
   Size:         37    1130     621

  Fragments arranged by size: 

                1130     621      37
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     888    1788 
   Size:        888     900
 
BtsI GCAGTG_nn'

Cuts at:      0     462    1477    1788 
   Size:        462    1015     311

  Fragments arranged by size: 

                1015     462     311
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     347    1788 
   Size:        347    1441
 
EaeI y'GGCC_r

Cuts at:      0      95    1493    1788 
   Size:         95    1398     295

  Fragments arranged by size: 

                1398     295      95
 
EagI C'GGCC_G

Cuts at:      0      95    1788 
   Size:         95    1693
 
EarI CTCTTCn'nnn_

Cuts at:      0     629     830    1553    1731    1788 
   Size:        629     201     723     178      57

  Fragments arranged by size: 

                 723     629     201     178      57
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     853     876    1036    1126    1406    1788 
   Size:        271     582      23     160      90     280     382

  Fragments arranged by size: 

                 582     382     280     271     160      90      23
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1584    1788 
   Size:       1584     204
 
EcoO109I rG'GnC_Cy

Cuts at:      0      88    1131    1539    1788 
   Size:         88    1043     408     249

  Fragments arranged by size: 

                1043     408     249      88
 
EcoRI G'AATT_C

Cuts at:      0    1400    1788 
   Size:       1400     388
 
EcoRV GAT'ATC

Cuts at:      0     927    1056    1788 
   Size:        927     129     732

  Fragments arranged by size: 

                 927     732     129
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     844     991    1788 
   Size:        844     147     797

  Fragments arranged by size: 

                 844     797     147
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     373     405     652     684    1788 
   Size:        373      32     247      32    1104

  Fragments arranged by size: 

                1104     373     247      32      32
 
HincII GTy'rAC

Cuts at:      0     425     698    1788 
   Size:        425     273    1090

  Fragments arranged by size: 

                1090     425     273
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      92    1788 
   Size:         92    1696
 
MslI CAynn'nnrTG

Cuts at:      0     487     757    1001    1681    1788 
   Size:        487     270     244     680     107

  Fragments arranged by size: 

                 680     487     270     244     107
 
MspA1I CmG'CkG

Cuts at:      0      39     810    1788 
   Size:         39     771     978

  Fragments arranged by size: 

                 978     771      39
 
NcoI C'CATG_G

Cuts at:      0    1167    1788 
   Size:       1167     621
 
NspI r_CATG'y

Cuts at:      0    1680    1788 
   Size:       1680     108
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1564    1596    1788 
   Size:       1564      32     192

  Fragments arranged by size: 

                1564     192      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1131    1788 
   Size:       1131     657
 
PstI C_TGCA'G

Cuts at:      0    1325    1538    1788 
   Size:       1325     213     250

  Fragments arranged by size: 

                1325     250     213
 
PvuII CAG'CTG

Cuts at:      0     810    1788 
   Size:        810     978
 
SacII CC_GC'GG

Cuts at:      0      40    1788 
   Size:         40    1748
 
SanDI GG'GwC_CC

Cuts at:      0    1131    1788 
   Size:       1131     657
 
SapI GCTCTTCn'nnn_

Cuts at:      0     830    1731    1788 
   Size:        830     901      57

  Fragments arranged by size: 

                 901     830      57
 
SbfI CC_TGCA'GG

Cuts at:      0    1325    1788 
   Size:       1325     463
 
SexAI A'CCwGG_T

Cuts at:      0    1271    1788 
   Size:       1271     517
 
SfcI C'TryA_G

Cuts at:      0     786     798    1321    1534    1788 
   Size:        786      12     523     213     254

  Fragments arranged by size: 

                 786     523     254     213      12
 
SmlI C'TyrA_G

Cuts at:      0     880    1788 
   Size:        880     908
 
SphI G_CATG'C

Cuts at:      0    1680    1788 
   Size:       1680     108
 
StuI AGG'CCT

Cuts at:      0     595    1788 
   Size:        595    1193
 
StyI C'CwwG_G

Cuts at:      0     299    1167    1788 
   Size:        299     868     621

  Fragments arranged by size: 

                 868     621     299
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1173    1205    1788 
   Size:       1173      32     583

  Fragments arranged by size: 

                1173     583      32
 
XhoI C'TCGA_G

Cuts at:      0     880    1788 
   Size:        880     908
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI    AlwNI    ApaLI     ApoI     AvaI    BamHI
    BanII     BbsI     BcgI     BclI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BpmI   Bpu10I    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI   BstEII    BstYI     BtgI    BtgZI     BtsI     DrdI     EaeI
     EagI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
    Hin4I   HincII     MmeI     MslI   MspA1I     NcoI     NspI     PpiI
    PpuMI     PstI    PvuII    SacII    SanDI     SapI     SbfI    SexAI
     SfcI     SmlI     SphI     StuI     StyI     TstI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI     BanI     BbeI    BbvCI    BciVI    BfrBI     BglI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsiWI
    BsmBI    BspEI    BspHI    BsrGI   BssHII    BstBI    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI     DraI   DraIII     EciI  EcoICRI     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI     SalI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com