Restriction map for pF1KB4576
##################################
# NO CUT ENZYMES (  169 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BbeI        BbrPI       BciVI       BcnI        BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiWI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        Eam1105I    Eco47III    Eco81I      EcoNI       
EcoO109I    EcoRI       EcoRV       EcoT14I     EcoT22I     
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HinfI       HpaI        KasI        KpnI        
KspI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MspA1I      MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AlwNI         186  CAGGGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         211  GTGCTC               G[AT]GC[AT]^C
BamHI         479  GGATCC               G^GATCC
BanI          322  GGTGCC               G^G[CT][GA]CC
BbvCI         330  CCTCAGC              CCTCAGC(-5/-2)
BcgI           74  GCAAGATCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          344  TGATCA               T^GATCA
BfaI          451  CTAG                 C^TAG
BseRI         328  CTCCTC               GAGGAG(10/8)
BsiEI         546  CGGCCG               CG[GA][CT]^CG
BsiHKAI       211  GTGCTC               G[AT]GC[AT]^C
BsmFI         471  GTCCC                GGGAC(10/14)
BsrI          426  CCAGT                ACTGG(1/-1)
BssSI          52  CTCGTG               CACGAG(-5/-1)
BstEII        288  GGTGACC              G^GT[ACGT]ACC
BstPI         288  GGTGACC              G^GT[ACGT]ACC
CfoI          238  GCGC                 GCG^C
EagI          546  CGGCCG               C^GGCCG
EarI          214  CTCTTC               CTCTTC(1/4)
EclXI         546  CGGCCG               C^GGCCG
Eco52I        546  CGGCCG               C^GGCCG
EcoO65I       288  GGTGACC              G^GT[ACGT]ACC
FbaI          344  TGATCA               T^GATCA
HaeII         237  GGCGCT               [GA]GCGC^[CT]
HapII         221  CCGG                 C^CGG
HhaI          238  GCGC                 GCG^C
HinP1I        238  GCGC                 G^CGC
HindIII       407  AAGCTT               A^AGCTT
HpaII         221  CCGG                 C^CGG
Ksp632I       214  CTCTTC               CTCTTC(1/4)
MaeI          451  CTAG                 C^TAG
MspI          221  CCGG                 C^CGG
NheI          450  GCTAGC               G^CTAGC
PflMI         426  CCAGTTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI          213  GCTCTTC              GCTCTTC(1/4)
TaqI          387  TCGA                 T^CGA
TspRI         441  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       387  TCGA                 T^CGA
Van91I        426  CCAGTTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BanII         181  GGGCTC               G[GA]GC[CT]^C
              281  GAGCCC              
BbsI           66  GAAGAC               GAAGAC(2/6)
              551  GAAGAC              
BbvI           12  GCAGC                GCAGC(8/12)
              486  GCTGC               
BpuAI          66  GAAGAC               GAAGAC(2/6)
              551  GAAGAC              
BsiYI          81  CCTCGGCGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              426  CCAGTTCTTGG         
BslI           81  CCTCGGCGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              426  CCAGTTCTTGG         
BsmAI         457  GAGAC                GTCTC(1/5)
              202  GTCTC               
BsmBI         201  CGTCTC               CGTCTC(1/5)
              457  GAGACG              
BsrDI         225  CATTGC               GCAATG(2/0)
              146  GCAATG              
BstF5I        306  CATCC                GGATG(2/0)
               99  GGATG               
BstYI          77  AGATCC               [GA]^GATC[CT]
              479  GGATCC              
EaeI            5  CGGCCA               [CT]^GGCC[GA]
              546  CGGCCG              
FokI          306  CATCC                GGATG(9/13)
               99  GGATG               
HphI          288  GGTGA                GGTGA(8/7)
              401  TCACC               
MaeIII        289  GTGAC                ^GT[ACGT]AC
              439  GTCAC               
MflI           77  AGATCC               [GA]^GATC[CT]
              479  GGATCC              
TseI           12  GCAGC                G^C[AT]GC
              486  GCTGC               
Tsp45I        289  GTGAC                ^GT[GC]AC
              439  GTCAC               
XhoII          77  AGATCC               [GA]^GATC[CT]
              479  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BglI        
BmyI        BsaJI       Bsp1286I    BssKI       BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EcoRII      
FauI        Fnu4HI      HaeIII      ItaI        MboI        
MboII       MnlI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
SfaNI       
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