Restriction map for pF1KB4576
 (Circular) (Six-base) MAPSORT of: pF1KB4576.seq  Check: 4082  from: 1  to: 3680



pF1KB4576  3680 bp

 With 182 enzymes: SgfI * 

                        February 15, 2010 17:49  ..

AccI GT'mk_AC

Cuts at:    718     718 
   Size:       3680
 
Acc65I G'GTAC_C

Cuts at:    696     696 
   Size:       3680
 
AclI AA'CG_TT

Cuts at:   3520    3520 
   Size:       3680
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    193    1505    1937    2684    2952     193 
   Size:       1312     432     747     268     921

  Fragments arranged by size: 

                1312     921     747     432     268
 
AflIII A'CryG_T

Cuts at:   1032    2136    3113    1032 
   Size:       1104     977    1599

  Fragments arranged by size: 

                1599    1104     977
 
AgeI A'CCGG_T

Cuts at:   2068    2068 
   Size:       3680
 
AlwNI CAG_nnn'CTG

Cuts at:    299     985    2552     299 
   Size:        686    1567    1427

  Fragments arranged by size: 

                1567    1427     686
 
ApaLI G'TGCA_C

Cuts at:   2450    2450 
   Size:       3680
 
ApoI r'AATT_y

Cuts at:    684    3033    3289     684 
   Size:       2349     256    1075

  Fragments arranged by size: 

                2349    1075     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3680
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3680
 
AvaI C'yCGr_G

Cuts at:    700     700 
   Size:       3680
 
BamHI G'GATC_C

Cuts at:    587     705     587 
   Size:        118    3562
 
BanI G'GyrC_C

Cuts at:    430     696    1110    1358    1393     430 
   Size:        266     414     248      35    2717

  Fragments arranged by size: 

                2717     414     266     248      35
 
BanII G_rGCy'C

Cuts at:    293     393     694    1724     293 
   Size:        100     301    1030    2249

  Fragments arranged by size: 

                2249    1030     301     100
 
BbeI G_GCGC'C

Cuts at:   1114    1362    1114 
   Size:        248    3432
 
BbsI GAAGACnn'nnnn_

Cuts at:    181     666     181 
   Size:        485    3195
 
BbvCI CC'TCA_GC

Cuts at:    439     439 
   Size:       3680
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    171     205     171 
   Size:         34    3646
 
BciVI GTATCCnnnnn_n'

Cuts at:   1578    2339    3680    1578 
   Size:        761    1341    1578

  Fragments arranged by size: 

                1578    1341     761
 
BclI T'GATC_A

Cuts at:    452    1200     452 
   Size:        748    2932
 
BglI GCCn_nnn'nGGC

Cuts at:    112     288     343     425     112 
   Size:        176      55      82    3367

  Fragments arranged by size: 

                3367     176      82      55
 
BglII A'GATC_T

Cuts at:   1195    3232    1195 
   Size:       2037    1643
 
BlpI GC'TnA_GC

Cuts at:    792     792 
   Size:       3680
 
Bme1580I G_kGCm'C

Cuts at:    435    1305    1398    2454     435 
   Size:        870      93    1056    1661

  Fragments arranged by size: 

                1661    1056     870      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    892     973    1308    3452     892 
   Size:         81     335    2144    1120

  Fragments arranged by size: 

                2144    1120     335      81
 
BmtI G_CTAG'C

Cuts at:    562     562 
   Size:       3680
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2047    2047 
   Size:       3680
 
Bpu10I CC'TnA_GC

Cuts at:    292     439     292 
   Size:        147    3533
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    856    1735    2227    2525     856 
   Size:        879     492     298    2011

  Fragments arranged by size: 

                2011     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3384    3384 
   Size:       3680
 
BsaAI yAC'GTr

Cuts at:   1033    1033 
   Size:       3680
 
BsaBI GATnn'nnATC

Cuts at:      6    1194    3231       6 
   Size:       1188    2037     455

  Fragments arranged by size: 

                2037    1188     455
 
BsaHI Gr'CG_yC

Cuts at:   1111    1359    2061    3557    1111 
   Size:        248     702    1496    1234

  Fragments arranged by size: 

                1496    1234     702     248
 
BsaWI w'CCGG_w

Cuts at:    869    1390    2068    2342    2489    2900     869 
   Size:        521     678     274     147     411    1649

  Fragments arranged by size: 

                1649     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    380     410     380 
   Size:         30    3650
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    427     427 
   Size:       3680
 
BseYI C'CCAG_C

Cuts at:   1105    1863    2440    2936    1105 
   Size:        758     577     496    1849

  Fragments arranged by size: 

                1849     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     657    1222    1268    2476     104 
   Size:        553     565      46    1208    1308

  Fragments arranged by size: 

                1308    1208     565     553      46
 
BsiHKAI G_wGCw'C

Cuts at:    323     694    1472    1662    2454     323 
   Size:        371     778     190     792    1549

  Fragments arranged by size: 

                1549     792     778     371     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    315     559     315 
   Size:        244    3436
 
Bsp1286I G_dGCh'C

Cuts at:    293     323     393     435     694    1305    1398    1472    1662 
   Size:         30      70      42     259     611      93      74     190

Cuts at:   1662    1724    2454     293 
   Size:         62     730    1519

  Fragments arranged by size: 

                1519     730     611     259     190      93      74      70
                  62      42      30
 
BspEI T'CCGG_A

Cuts at:   2900    2900 
   Size:       3680
 
BspHI T'CATG_A

Cuts at:   2856    2856 
   Size:       3680
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    730     944    1246    1627     730 
   Size:        214     302     381    2783

  Fragments arranged by size: 

                2783     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1972    2026    3510    3675      50 
   Size:       1922      54    1484     165      55

  Fragments arranged by size: 

                1922    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    261     332    1592     261 
   Size:         71    1260    2349

  Fragments arranged by size: 

                2349    1260      71
 
BsrFI r'CCGG_y

Cuts at:   1678    1859    2068    1678 
   Size:        181     209    3290

  Fragments arranged by size: 

                3290     209     181
 
BssSI C'ACGA_G

Cuts at:    160    1951    2309     160 
   Size:       1791     358    1531

  Fragments arranged by size: 

                1791    1531     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1057    1057 
   Size:       3680
 
BstBI TT'CG_AA

Cuts at:   2041    2041 
   Size:       3680
 
BstEII G'GTnAC_C

Cuts at:    396     396 
   Size:       3680
 
BstYI r'GATC_y

Cuts at:      1     185     587     705    1172    1195    1530    1776    2788 
   Size:        184     402     118     467      23     335     246    1012

Cuts at:   2788    2874    2886    3232       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     467     449     402     346     335     246     184
                 118      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3680
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    211    1630    1811    1974     211 
   Size:       1419     181     163    1917

  Fragments arranged by size: 

                1917    1419     181     163
 
BtsI GCAGTG_nn'

Cuts at:    548     923     548 
   Size:        375    3305
 
DraI TTT'AAA

Cuts at:    679     679 
   Size:       3680
 
DrdI GACnn_nn'nnGTC

Cuts at:   1386    2244    1386 
   Size:        858    2822
 
EaeI y'GGCC_r

Cuts at:    113     654    1265    1439    1830    1857    3202     113 
   Size:        541     611     174     391      27    1345     591

  Fragments arranged by size: 

                1345     611     591     541     391     174      27
 
EagI C'GGCC_G

Cuts at:    654    1265     654 
   Size:        611    3069
 
EarI CTCTTCn'nnn_

Cuts at:    328    1703    1913     328 
   Size:       1375     210    2095

  Fragments arranged by size: 

                2095    1375     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    406    1888    2198    2344    3490     406 
   Size:       1482     310     146    1146     596

  Fragments arranged by size: 

                1482    1146     596     310     146
 
EcoICRI GAG'CTC

Cuts at:    692     692 
   Size:       3680
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    193    1505    1937    2684    2952     193 
   Size:       1312     432     747     268     921

  Fragments arranged by size: 

                1312     921     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    819     819 
   Size:       3680
 
EcoRI G'AATT_C

Cuts at:    684     684 
   Size:       3680
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1699    1699 
   Size:       3680
 
FspI TGC'GCA

Cuts at:   1461    1461 
   Size:       3680
 
HaeII r_GCGC'y

Cuts at:    349    1114    1362    2384    3363     349 
   Size:        765     248    1022     979     666

  Fragments arranged by size: 

                1022     979     765     666     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1522    1552    1554    1584    1522 
   Size:         30       2      30    3618

  Fragments arranged by size: 

                3618      30      30       2
 
HincII GTy'rAC

Cuts at:    719     719 
   Size:       3680
 
HindIII A'AGCT_T

Cuts at:    515     515 
   Size:       3680
 
KasI G'GCGC_C

Cuts at:   1110    1358    1110 
   Size:        248    3432
 
KpnI G_GTAC'C

Cuts at:    700     700 
   Size:       3680
 
MluI A'CGCG_T

Cuts at:   3113    3113 
   Size:       3680
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2351    2535    2351 
   Size:        184    3496
 
MscI TGG'CCA

Cuts at:   1441    1441 
   Size:       3680
 
MslI CAynn'nnrTG

Cuts at:   1669    1796    1669 
   Size:        127    3553
 
MspA1I CmG'CkG

Cuts at:    791    1105    1465    2478    2723     791 
   Size:        314     360    1013     245    1748

  Fragments arranged by size: 

                1748    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1861    1861 
   Size:       3680
 
NarI GG'CG_CC

Cuts at:   1111    1359    1111 
   Size:        248    3432
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3680
 
NgoMIV G'CCGG_C

Cuts at:   1859    1859 
   Size:       3680
 
NheI G'CTAG_C

Cuts at:    558     558 
   Size:       3680
 
NspI r_CATG'y

Cuts at:    733    1233    2140     733 
   Size:        500     907    2273

  Fragments arranged by size: 

                2273     907     500
 
PasI CC'CwG_GG

Cuts at:    582     582 
   Size:       3680
 
PciI A'CATG_T

Cuts at:   2136    2136 
   Size:       3680
 
PflMI CCAn_nnn'nTGG

Cuts at:    540     540 
   Size:       3680
 
PmeI GTTT'AAAC

Cuts at:    679     679 
   Size:       3680
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2819    2851    2819 
   Size:         32    3648
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    454     486     454 
   Size:         32    3648
 
PstI C_TGCA'G

Cuts at:    727    1412     727 
   Size:        685    2995
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3680
 
PvuII CAG'CTG

Cuts at:   1105    1465    1105 
   Size:        360    3320
 
RsrII CG'GwC_CG

Cuts at:   1875    1875 
   Size:       3680
 
SacI G_AGCT'C

Cuts at:    694     694 
   Size:       3680
 
SalI G'TCGA_C

Cuts at:    717     717 
   Size:       3680
 
SapI GCTCTTCn'nnn_

Cuts at:    328    1703    1913     328 
   Size:       1375     210    2095

  Fragments arranged by size: 

                2095    1375     210
 
SbfI CC_TGCA'GG

Cuts at:    727     727 
   Size:       3680
 
ScaI AGT'ACT

Cuts at:   3197    3197 
   Size:       3680
 
SfcI C'TryA_G

Cuts at:     33     723    1408    2401    2592      33 
   Size:        690     685     993     191    1121

  Fragments arranged by size: 

                1121     993     690     685     191
 
SfoI GGC'GCC

Cuts at:   1112    1360    1112 
   Size:        248    3432
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3680
 
SmaI CCC'GGG

Cuts at:    702     702 
   Size:       3680
 
SmlI C'TyrA_G

Cuts at:    835    1750    2242    2504     835 
   Size:        915     492     262    2011

  Fragments arranged by size: 

                2011     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3680
 
SphI G_CATG'C

Cuts at:    733    1233     733 
   Size:        500    3180
 
StyI C'CwwG_G

Cuts at:    107     814    1168     107 
   Size:        707     354    2619

  Fragments arranged by size: 

                2619     707     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1263    1396    1855    2064    1263 
   Size:        133     459     209    2879

  Fragments arranged by size: 

                2879     459     209     133
 
TatI w'GTAC_w

Cuts at:   3195    3195 
   Size:       3680
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    380     412    3197    3229     380 
   Size:         32    2785      32     831

  Fragments arranged by size: 

                2785     831      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1477    1477 
   Size:       3680
 
XbaI T'CTAG_A

Cuts at:    711     711 
   Size:       3680
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1175    1175 
   Size:       3680
 
XmaI C'CCGG_G

Cuts at:    700     700 
   Size:       3680
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NspI     PasI     PciI    PflMI
     PmeI     PpiI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BfrBI    BmgBI     BpmI     BsgI
    BsiWI     BsmI    BsrGI   BssHII    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI     HpaI     MfeI
     NdeI     NotI     NruI     NsiI     PacI     PfoI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI
     ZraI
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