Restriction map for pF1KB3324
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       AccII       AclI        AcyI        AflII       
AgeI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        AspI        
AviII       BamHI       BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfrI        BglI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DrdI        Eco47III    Eco81I      
EcoNI       EcoRV       EcoT22I     FauI        FseI        
FspI        HaeII       HgaI        Hin1I       HincII      
HindII      HpaI        KasI        KspI        MamI        
MfeI        MluI        MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SspBI       
SspI        SwaI        Tth111I     XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I        644  GGTACC               G^GTACC
AflIII       1130  ACACGT               A^C[GA][CT]GT
AhdI         1632  GACACTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      1150  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       644  GGTACC               G^GTACC
AspEI        1632  GACACTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          557  CTCGGG               C^[CT]CG[GA]G
AvrII         196  CCTAGG               C^CTAGG
BalI          317  TGGCCA               TGG^CCA
BanI          644  GGTACC               G^G[CT][GA]CC
BcnI            6  CCCGG                CC^[GC]GG
BfaI          197  CTAG                 C^TAG
BglII         977  AGATCT               A^GATCT
BlnI          196  CCTAGG               C^CTAGG
BsaI           62  GGTCTC               GGTCTC(1/5)
BseRI         785  CTCCTC               GAGGAG(10/8)
BsgI         1159  CTGCAC               GTGCAG(16/14)
BsiEI           8  CGGCCG               CG[GA][CT]^CG
BsoBI         557  CTCGGG               C^[CT]CG[GA]G
BspMI        1192  ACCTGC               ACCTGC(4/8)
BstAPI        286  GCAAGCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1188  GGTGACC              G^GT[ACGT]ACC
BstPI        1188  GGTGACC              G^GT[ACGT]ACC
CfoI          137  GCGC                 GCG^C
DraIII        611  CACTATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1088  CCACGG               C^C[GA][CT]GG
EagI            8  CGGCCG               C^GGCCG
Eam1105I     1632  GACACTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI           8  CGGCCG               C^GGCCG
Eco52I          8  CGGCCG               C^GGCCG
EcoO65I      1188  GGTGACC              G^GT[ACGT]ACC
EcoT14I       196  CCTAGG               C^C[AT][AT]GG
HhaI          137  GCGC                 GCG^C
HinP1I        137  GCGC                 G^CGC
HindIII       679  AAGCTT               A^AGCTT
KpnI          644  GGTACC               GGTAC^C
MaeI          197  CTAG                 C^TAG
MaeII        1132  ACGT                 A^CGT
MluNI         317  TGGCCA               TGG^CCA
MscI          317  TGGCCA               TGG^CCA
NciI            6  CCCGG                CC^[GC]GG
PflMI         127  CCAGGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1378  AGGACCT              [GA]G^G[AT]CC[CT]
PstI         1625  CTGCAG               CTGCA^G
SbfI         1624  CCTGCAGG             CCTGCA^GG
ScaI         1360  AGTACT               AGT^ACT
SmlI           37  CTCAAG               C^T[CT][GA]AG
Sse8387I     1624  CCTGCAGG             CCTGCA^GG
StuI          720  AGGCCT               AGG^CCT
StyI          196  CCTAGG               C^C[AT][AT]GG
Van91I        127  CCAGGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1150  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI         1294  GTAGAC               GT^[AC][GT]AC
             1640  GTCTAC              
AccIII       1253  TCCGGA               T^CCGGA
             1663  TCCGGA              
AlwNI         128  CAGGGACTG            CAG[ACGT][ACGT][ACGT]^CTG
              252  CAGACTCTG           
BclI          936  TGATCA               T^GATCA
             1064  TGATCA              
BsaWI        1253  TCCGGA               [AT]^CCGG[AT]
             1663  TCCGGA              
BseAI        1253  TCCGGA               T^CCGGA
             1663  TCCGGA              
BspEI        1253  TCCGGA               T^CCGGA
             1663  TCCGGA              
BsrDI        1212  CATTGC               GCAATG(2/0)
             1404  CATTGC              
BstYI         977  AGATCT               [GA]^GATC[CT]
             1593  GGATCT              
DraII        1378  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1564  GGGGCCT             
EaeI            8  CGGCCG               [CT]^GGCC[GA]
              317  TGGCCA              
EarI          734  GAAGAG               CTCTTC(1/4)
             1667  GAAGAG              
EcoO109I     1378  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1564  GGGGCCT             
FbaI          936  TGATCA               T^GATCA
             1064  TGATCA              
Ksp632I       734  GAAGAG               CTCTTC(1/4)
             1667  GAAGAG              
MaeIII        652  GTGAC                ^GT[ACGT]AC
             1189  GTGAC               
MflI          977  AGATCT               [GA]^GATC[CT]
             1593  GGATCT              
MroI         1253  TCCGGA               T^CCGGA
             1663  TCCGGA              
MspA1I        243  CAGCTG               C[AC]G^C[GT]G
             1757  CAGCTG              
PleI          254  GACTC                GAGTC(4/5)
              436  GAGTC               
PvuII         243  CAGCTG               CAG^CTG
             1757  CAGCTG              
SfcI         1625  CTGCAG               C^T[GA][CT]AG
             1681  CTACAG              
SphI           98  GCATGC               GCATG^C
              665  GCATGC              
Tsp45I        652  GTGAC                ^GT[GC]AC
             1189  GTGAC               
XhoII         977  AGATCT               [GA]^GATC[CT]
             1593  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BbsI        BbvI        
BmyI        BpmI        BpuAI       BsaJI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EcoRI       
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MboI        
MboII       MnlI        MseI        MslI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     
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