Restriction map for pF1KB6181
 (Linear) (Six-base) MAPSORT of: KIBB6181.seq  Check: 6384  from: 1  to: 1977



KIBB6181.orf

 With 182 enzymes: SgfI * 

                        July 28, 2009 13:23  ..

AatII G_ACGT'C

Cuts at:      0    1488    1977 
   Size:       1488     489
 
AccI GT'mk_AC

Cuts at:      0    1808    1977 
   Size:       1808     169
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1213    1645    1977 
   Size:       1213     432     332

  Fragments arranged by size: 

                1213     432     332
 
AlwNI CAG_nnn'CTG

Cuts at:      0     210    1977 
   Size:        210    1767
 
ApaI G_GGCC'C

Cuts at:      0     688    1977 
   Size:        688    1289
 
ApoI r'AATT_y

Cuts at:      0     583    1607    1936    1977 
   Size:        583    1024     329      41

  Fragments arranged by size: 

                1024     583     329      41
 
AseI AT'TA_AT

Cuts at:      0    1180    1977 
   Size:       1180     797
 
BanI G'GyrC_C

Cuts at:      0      49      86    1977 
   Size:         49      37    1891

  Fragments arranged by size: 

                1891      49      37
 
BanII G_rGCy'C

Cuts at:      0     681     688    1977 
   Size:        681       7    1289

  Fragments arranged by size: 

                1289     681       7
 
BbeI G_GCGC'C

Cuts at:      0      90    1977 
   Size:         90    1887
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     750     876    1977 
   Size:        750     126    1101

  Fragments arranged by size: 

                1101     750     126
 
BclI T'GATC_A

Cuts at:      0    1326    1977 
   Size:       1326     651
 
Bme1580I G_kGCm'C

Cuts at:      0     688    1977 
   Size:        688    1289
 
BmgBI CAC'GTC

Cuts at:      0    1812    1977 
   Size:       1812     165
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1761    1977 
   Size:       1761     216
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     487    1977 
   Size:        487    1490
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1310    1977 
   Size:       1310     667
 
Bpu10I CC'TnA_GC

Cuts at:      0     680    1977 
   Size:        680    1297
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     177    1021    1088    1977 
   Size:        177     844      67     889

  Fragments arranged by size: 

                 889     844     177      67
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1236    1364    1524    1977 
   Size:       1236     128     160     453

  Fragments arranged by size: 

                1236     453     160     128
 
BsaBI GATnn'nnATC

Cuts at:      0    1202    1977 
   Size:       1202     775
 
BsaHI Gr'CG_yC

Cuts at:      0      87    1485    1977 
   Size:         87    1398     492

  Fragments arranged by size: 

                1398     492      87
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1250    1280    1977 
   Size:       1250      30     697

  Fragments arranged by size: 

                1250     697      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      27     505    1977 
   Size:         27     478    1472

  Fragments arranged by size: 

                1472     478      27
 
BseYI C'CCAG_C

Cuts at:      0     687    1129    1679    1977 
   Size:        687     442     550     298

  Fragments arranged by size: 

                 687     550     442     298
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88    1817    1977 
   Size:         88    1729     160

  Fragments arranged by size: 

                1729     160      88
 
BsiHKAI G_wGCw'C

Cuts at:      0     681    1977 
   Size:        681    1296
 
BsmI GAATG_Cn'

Cuts at:      0    1091    1792    1977 
   Size:       1091     701     185

  Fragments arranged by size: 

                1091     701     185
 
Bsp1286I G_dGCh'C

Cuts at:      0     681     688    1977 
   Size:        681       7    1289

  Fragments arranged by size: 

                1289     681       7
 
BspHI T'CATG_A

Cuts at:      0    1896    1977 
   Size:       1896      81
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1916    1977 
   Size:       1916      61
 
BsrFI r'CCGG_y

Cuts at:      0    1153    1977 
   Size:       1153     824
 
BstBI TT'CG_AA

Cuts at:      0     741    1977 
   Size:        741    1236
 
BstEII G'GTnAC_C

Cuts at:      0     985    1977 
   Size:        985     992
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     372    1753    1977 
   Size:        372    1381     224

  Fragments arranged by size: 

                1381     372     224
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     963     998    1977 
   Size:        963      35     979

  Fragments arranged by size: 

                 979     963      35
 
DraI TTT'AAA

Cuts at:      0    1202    1977 
   Size:       1202     775
 
EaeI y'GGCC_r

Cuts at:      0    1151    1977 
   Size:       1151     826
 
EarI CTCTTCn'nnn_

Cuts at:      0    1864    1977 
   Size:       1864     113
 
EcoICRI GAG'CTC

Cuts at:      0     679    1977 
   Size:        679    1298
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1213    1310    1645    1977 
   Size:       1213      97     335     332

  Fragments arranged by size: 

                1213     335     332      97
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     487    1502    1977 
   Size:        487    1015     475

  Fragments arranged by size: 

                1015     487     475
 
EcoO109I rG'GnC_Cy

Cuts at:      0     684    1977 
   Size:        684    1293
 
EcoRI G'AATT_C

Cuts at:      0    1936    1977 
   Size:       1936      41
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     390    1650    1977 
   Size:        390    1260     327

  Fragments arranged by size: 

                1260     390     327
 
HaeII r_GCGC'y

Cuts at:      0      90    1977 
   Size:         90    1887
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     362     394    1250    1282    1977 
   Size:        362      32     856      32     695

  Fragments arranged by size: 

                 856     695     362      32      32
 
HincII GTy'rAC

Cuts at:      0     662    1809    1977 
   Size:        662    1147     168

  Fragments arranged by size: 

                1147     662     168
 
HindIII A'AGCT_T

Cuts at:      0     503    1977 
   Size:        503    1474
 
HpaI GTT'AAC

Cuts at:      0     662    1977 
   Size:        662    1315
 
KasI G'GCGC_C

Cuts at:      0      86    1977 
   Size:         86    1891
 
MfeI C'AATT_G

Cuts at:      0     671    1977 
   Size:        671    1306
 
MslI CAynn'nnrTG

Cuts at:      0    1916    1977 
   Size:       1916      61
 
MspA1I CmG'CkG

Cuts at:      0     561    1977 
   Size:        561    1416
 
NarI GG'CG_CC

Cuts at:      0      87    1977 
   Size:         87    1890
 
NdeI CA'TA_TG

Cuts at:      0    1913    1977 
   Size:       1913      64
 
PsiI TTA'TAA

Cuts at:      0     518    1318    1977 
   Size:        518     800     659

  Fragments arranged by size: 

                 800     659     518
 
PspOMI G'GGCC_C

Cuts at:      0     684    1977 
   Size:        684    1293
 
PvuII CAG'CTG

Cuts at:      0     561    1977 
   Size:        561    1416
 
SacI G_AGCT'C

Cuts at:      0     681    1977 
   Size:        681    1296
 
SalI G'TCGA_C

Cuts at:      0    1807    1977 
   Size:       1807     170
 
ScaI AGT'ACT

Cuts at:      0      19    1190    1622    1903    1977 
   Size:         19    1171     432     281      74

  Fragments arranged by size: 

                1171     432     281      74      19
 
SexAI A'CCwGG_T

Cuts at:      0    1281    1977 
   Size:       1281     696
 
SfoI GGC'GCC

Cuts at:      0      88    1977 
   Size:         88    1889
 
SmlI C'TyrA_G

Cuts at:      0     192    1036    1103    1977 
   Size:        192     844      67     874

  Fragments arranged by size: 

                 874     844     192      67
 
StyI C'CwwG_G

Cuts at:      0    1545    1977 
   Size:       1545     432
 
SwaI ATTT'AAAT

Cuts at:      0    1202    1977 
   Size:       1202     775
 
TatI w'GTAC_w

Cuts at:      0      17    1042    1188    1260    1620    1901    1977 
   Size:         17    1025     146      72     360     281      76

  Fragments arranged by size: 

                1025     360     281     146      76      72      17
 
Tth111I GACn'n_nGTC

Cuts at:      0    1642    1977 
   Size:       1642     335
 
XbaI T'CTAG_A

Cuts at:      0    1059    1977 
   Size:       1059     918
 
XmnI GAAnn'nnTTC

Cuts at:      0     361    1437    1977 
   Size:        361    1076     540

  Fragments arranged by size: 

                1076     540     361
 
ZraI GAC'GTC

Cuts at:      0    1486    1977 
   Size:       1486     491
 

 Enzymes that do cut:

    AatII     AccI     AcuI    AlwNI     ApaI     ApoI     AseI     BanI
    BanII     BbeI     BbsI     BclI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrFI
    BstBI   BstEII    BtgZI    CspCI     DraI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     MfeI     MslI   MspA1I     NarI     NdeI
     PsiI   PspOMI    PvuII     SacI     SalI     ScaI    SexAI     SfoI
     SmlI     StyI     SwaI     TatI  Tth111I     XbaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI     BmtI    BsaAI    BsaWI    BsiEI    BsiWI    BsmBI    BspEI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI     BtsI     ClaI   DraIII     DrdI     EagI
     EciI    EcoRV     FseI     FspI    FspAI     KpnI     MluI     MmeI
     MscI     NaeI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI    PspXI     PsrI     PstI     PvuI    RsrII
    SacII    SanDI     SapI     SbfI     SfcI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI    TaqII
     TstI     XcmI     XhoI     XmaI
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