Restriction map for pF1KB5776
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        Asp700I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BbrPI       BfrI        BlnI        BsaAI       
BsiEI       BsiHKAI     BsiWI       Bsp1407I    BspDI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRV       FseI        
FspI        HindIII     HpaI        MluI        MluNI       
MscI        NcoI        NdeI        NheI        NotI        
NruI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PstI        PvuI        
PvuII       RsrII       SacI        SalI        SapI        
SbfI        SexAI       SfiI        SfuI        SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SwaI        Tth111I     XbaI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

Acc65I        504  GGTACC               G^GTACC
AccIII        129  TCCGGA               T^CCGGA
AclI          720  AACGTT               AA^CGTT
ApaI          979  GGGCCC               GGGCC^C
AseI         2073  ATTAAT               AT^TAAT
AsnI         2073  ATTAAT               AT^TAAT
Asp718I       504  GGTACC               G^GTACC
BamHI        1843  GGATCC               G^GATCC
BcgI          958  GCACCCGCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1649  TGATCA               T^GATCA
BcnI           40  CCGGG                CC^[GC]GG
BglI          103  GCCTCTTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2028  AGATCT               A^GATCT
BlpI            9  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I        9  GCTGAGC              GC^T[ACGT]AGC
BsaWI         129  TCCGGA               [AT]^CCGG[AT]
BseAI         129  TCCGGA               T^CCGGA
BseRI         133  GAGGAG               GAGGAG(10/8)
BsgI         1679  CTGCAC               GTGCAG(16/14)
BsmBI        1001  CGTCTC               CGTCTC(1/5)
BsmI         1877  GAATGC               GAATGC(1/-1)
BspEI         129  TCCGGA               T^CCGGA
BspHI        1752  TCATGA               T^CATGA
BspMI         507  ACCTGC               ACCTGC(4/8)
BsrDI        2159  CATTGC               GCAATG(2/0)
BstAPI        922  GCAGAGACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII           9  GCTGAGC              GC^T[ACGT]AGC
DsaI           98  CCGCGG               C^C[GA][CT]GG
EcoRI         596  GAATTC               G^AATTC
EcoT14I      1155  CCAAGG               C^C[AT][AT]GG
EcoT22I      1919  ATGCAT               ATGCA^T
FbaI         1649  TGATCA               T^GATCA
HgaI          913  GCGTC                GACGC(5/10)
HincII        165  GTTGAC               GT[CT]^[GA]AC
HindII        165  GTTGAC               GT[CT]^[GA]AC
KpnI          504  GGTACC               GGTAC^C
KspI           98  CCGCGG               CCGC^GG
MfeI          620  CAATTG               C^AATTG
MroI          129  TCCGGA               T^CCGGA
MunI          620  CAATTG               C^AATTG
NciI           40  CCGGG                CC^[GC]GG
NsiI         1919  ATGCAT               ATGCA^T
PaeR7I       1335  CTCGAG               C^TCGAG
PflMI        1847  CCATTTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         745  GACTTTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        2073  ATTAAT               AT^TAAT
Psp1406I      720  AACGTT               AA^CGTT
RcaI         1752  TCATGA               T^CATGA
SacII          98  CCGCGG               CCGC^GG
ScaI         1509  AGTACT               AGT^ACT
SfcI         1793  CTACAG               C^T[GA][CT]AG
SspI         1132  AATATT               AAT^ATT
StuI          239  AGGCCT               AGG^CCT
StyI         1155  CCAAGG               C^C[AT][AT]GG
Van91I       1847  CCATTTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1335  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI         1325  GTAGAC               GT^[AC][GT]AC
             1411  GTCTAC              
AccII          33  CGCG                 CG^CG
               99  CGCG                
AflIII        787  ACATGT               A^C[GA][CT]GT
             1527  ACATGT              
AlwNI          46  CAGGCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              923  CAGAGACTG           
AvaI           73  CCCGAG               C^[CT]CG[GA]G
             1335  CTCGAG              
BbvCI        1264  CCTCAGC              CCTCAGC(-5/-2)
             1474  CCTCAGC             
BciVI         351  GGATAC               GTATCC(6/5)
             2014  GGATAC              
BsoBI          73  CCCGAG               C^[CT]CG[GA]G
             1335  CTCGAG              
BspLU11I      787  ACATGT               A^CATGT
             1527  ACATGT              
BsrBI        2154  CCGCTC               CCGCTC(-3/-3)
               12  GAGCGG              
BstUI          33  CGCG                 CG^CG
               99  CGCG                
BstXI         422  CCACAGATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              619  CCAATTGGTTGG        
FauI          961  CCCGC                CCCGC(4/6)
             2153  CCCGC               
MaeII         721  ACGT                 A^CGT
             1235  ACGT                
MspA1I         98  CCGCGG               C[AC]G^C[GT]G
              777  CCGCTG              
MvnI           33  CGCG                 CG^CG
               99  CGCG                
PpuMI        1260  AGGACCT              [GA]G^G[AT]CC[CT]
             1816  GGGTCCC             
SgrAI          84  CGCCGGCG             C[GA]^CCGG[CT]G
               90  CGCCGGCG            
SmlI         1335  CTCGAG               C^T[CT][GA]AG
             1729  CTCAAG              
XcmI         1155  CCAAGGATTTCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1847  CCATTTTGTGGATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        ApoI        AvaII       BanI        BanII       
BbeI        BbsI        BbvI        BfaI        BmyI        
BpmI        BpuAI       BsaBI       BsaHI       BsaI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoO109I    EcoRII      Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeI        MaeIII      MamI        MboI        
MboII       MflI        MnlI        MseI        MslI        
MspI        MvaI        MwoI        NaeI        NarI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfoI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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