Restriction map for
pF1KB5776
(Circular) (Six-base) MAPSORT of: pF1KB5776.seq Check: 8548 from: 1 to: 5411
pF1KB5776 5411 bp
With 182 enzymes: SgfI *
July 22, 2009 17:02 ..
AccI GT'mk_AC
Cuts at: 1434 1520 2449 1434
Size: 86 929 4396
Fragments arranged by size:
4396 929 86
Acc65I G'GTAC_C
Cuts at: 612 2427 612
Size: 1815 3596
AclI AA'CG_TT
Cuts at: 829 5251 829
Size: 4422 989
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3236 3668 4415 4683 3236
Size: 432 747 268 3964
Fragments arranged by size:
3964 747 432 268
AflIII A'CryG_T
Cuts at: 895 1635 2763 3867 4844 895
Size: 740 1128 1104 977 1462
Fragments arranged by size:
1462 1128 1104 977 740
AgeI A'CCGG_T
Cuts at: 3799 3799
Size: 5411
AleI CACnn'nnGTG
Cuts at: 535 942 2285 535
Size: 407 1343 3661
Fragments arranged by size:
3661 1343 407
AlwNI CAG_nnn'CTG
Cuts at: 159 1036 2716 4283 159
Size: 877 1680 1567 1287
Fragments arranged by size:
1680 1567 1287 877
ApaI G_GGCC'C
Cuts at: 1091 1091
Size: 5411
ApaLI G'TGCA_C
Cuts at: 4181 4181
Size: 5411
ApoI r'AATT_y
Cuts at: 307 704 907 1094 1747 2234 2276 2415 4764
Size: 397 203 187 653 487 42 139 2349
Cuts at: 4764 5020 307
Size: 256 698
Fragments arranged by size:
2349 698 653 487 397 256 203 187
139 42
AseI AT'TA_AT
Cuts at: 20 2182 20
Size: 2162 3249
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5411
AvaI C'yCGr_G
Cuts at: 181 1443 2431 181
Size: 1262 988 3161
Fragments arranged by size:
3161 1262 988
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 596 629 596
Size: 33 5378
BamHI G'GATC_C
Cuts at: 1951 2436 1951
Size: 485 4926
BanI G'GyrC_C
Cuts at: 190 196 202 612 1968 2427 2841 3089 3124
Size: 6 6 410 1356 459 414 248 35
Cuts at: 3124 190
Size: 2477
Fragments arranged by size:
2477 1356 459 414 410 248 35 6
6
BanII G_rGCy'C
Cuts at: 182 1091 1711 2425 3455 182
Size: 909 620 714 1030 2138
Fragments arranged by size:
2138 1030 909 714 620
BbeI G_GCGC'C
Cuts at: 194 200 206 2845 3093 194
Size: 6 6 2639 248 2512
Fragments arranged by size:
2639 2512 248 6 6
BbsI GAAGACnn'nnnn_
Cuts at: 576 1412 1871 576
Size: 836 459 4116
Fragments arranged by size:
4116 836 459
BbvCI CC'TCA_GC
Cuts at: 1373 1583 1373
Size: 210 5201
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1055 1089 1055
Size: 34 5377
BciVI GTATCCnnnnn_n'
Cuts at: 453 2116 3309 4070 5411 453
Size: 1663 1193 761 1341 453
Fragments arranged by size:
1663 1341 1193 761 453
BclI T'GATC_A
Cuts at: 1757 2931 1757
Size: 1174 4237
BfrBI ATG'CAT
Cuts at: 2029 2029
Size: 5411
BglI GCCn_nnn'nGGC
Cuts at: 112 217 112
Size: 105 5306
BglII A'GATC_T
Cuts at: 2136 2926 4963 2136
Size: 790 2037 2584
Fragments arranged by size:
2584 2037 790
BlpI GC'TnA_GC
Cuts at: 118 2523 118
Size: 2405 3006
Bme1580I G_kGCm'C
Cuts at: 1091 3036 3129 4185 1091
Size: 1945 93 1056 2317
Fragments arranged by size:
2317 1945 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1689 2623 2704 3039 5183 1689
Size: 934 81 335 2144 1917
Fragments arranged by size:
2144 1917 934 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3778 3778
Size: 5411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 782 1349 1695 2117 364
Size: 418 567 346 422 3658
Fragments arranged by size:
3658 567 422 418 346
Bpu10I CC'TnA_GC
Cuts at: 840 1026 1373 1583 840
Size: 186 347 210 4668
Fragments arranged by size:
4668 347 210 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1822 2587 3466 3958 4256 1822
Size: 765 879 492 298 2977
Fragments arranged by size:
2977 879 765 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1181 1253 1735 1826 2211 5115 1181
Size: 72 482 91 385 2904 1477
Fragments arranged by size:
2904 1477 482 385 91 72
BsaAI yAC'GTr
Cuts at: 2764 2764
Size: 5411
BsaBI GATnn'nnATC
Cuts at: 6 1160 1197 1503 1997 2925 4962 6
Size: 1154 37 306 494 928 2037 455
Fragments arranged by size:
2037 1154 928 494 455 306 37
BsaHI Gr'CG_yC
Cuts at: 191 197 203 1021 2842 3090 3792 5288 191
Size: 6 6 818 1821 248 702 1496 314
Fragments arranged by size:
1821 1496 818 702 314 248 6 6
BsaWI w'CCGG_w
Cuts at: 237 2600 3121 3799 4073 4220 4631 237
Size: 2363 521 678 274 147 411 1017
Fragments arranged by size:
2363 1017 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 232 262 620 650 2145 2175 232
Size: 30 358 30 1495 30 3468
Fragments arranged by size:
3468 1495 358 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 256
Size: 5411
BseYI C'CCAG_C
Cuts at: 2836 3594 4171 4667 2836
Size: 758 577 496 3580
Fragments arranged by size:
3580 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1772 1772
Size: 5411
BsiEI CG_ry'CG
Cuts at: 104 2953 2999 4207 104
Size: 2849 46 1208 1308
Fragments arranged by size:
2849 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2425 3203 3393 4185 2425
Size: 778 190 792 3651
Fragments arranged by size:
3651 792 778 190
BsmI GAATG_Cn'
Cuts at: 1991 1991
Size: 5411
BsmBI CGTCTCn'nnnn_
Cuts at: 1115 1115
Size: 5411
Bsp1286I G_dGCh'C
Cuts at: 182 1091 1711 2425 3036 3129 3203 3393 3455
Size: 909 620 714 611 93 74 190 62
Cuts at: 3455 4185 182
Size: 730 1408
Fragments arranged by size:
1408 909 730 714 620 611 190 93
74 62
BspEI T'CCGG_A
Cuts at: 237 4631 237
Size: 4394 1017
BspHI T'CATG_A
Cuts at: 1860 4587 1860
Size: 2727 2684
BspMI ACCTGCnnnn'nnnn_
Cuts at: 624 2461 2675 2977 3358 624
Size: 1837 214 302 381 2677
Fragments arranged by size:
2677 1837 381 302 214
BsrBI CCG'CTC
Cuts at: 50 122 2264 3703 3757 5241 5406 50
Size: 72 2142 1439 54 1484 165 55
Fragments arranged by size:
2142 1484 1439 165 72 55 54
BsrDI GCAATG_nn'
Cuts at: 2266 3323 2266
Size: 1057 4354
BsrFI r'CCGG_y
Cuts at: 187 193 199 3409 3590 3799 187
Size: 6 6 3210 181 209 1799
Fragments arranged by size:
3210 1799 209 181 6 6
BssSI C'ACGA_G
Cuts at: 3682 4040 3682
Size: 358 5053
BstAPI GCAn_nnn'nTGC
Cuts at: 1036 2788 1036
Size: 1752 3659
BstBI TT'CG_AA
Cuts at: 3772 3772
Size: 5411
BstXI CCAn_nnnn'nTGG
Cuts at: 537 734 537
Size: 197 5214
BstYI r'GATC_y
Cuts at: 1 1498 1951 2136 2436 2903 2926 3261 3507
Size: 1497 453 185 300 467 23 335 246
Cuts at: 3507 4519 4605 4617 4963 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1497 1012 467 453 449 346 335 300
246 185 86 23 12
BtgI C'CryG_G
Cuts at: 107 206 107
Size: 99 5312
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3361 3542 3705 3361
Size: 181 163 5067
Fragments arranged by size:
5067 181 163
BtsI GCAGTG_nn'
Cuts at: 1556 2292 2654 1556
Size: 736 362 4313
Fragments arranged by size:
4313 736 362
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 294 329 294
Size: 35 5376
DraI TTT'AAA
Cuts at: 2410 2410
Size: 5411
DrdI GACnn_nn'nnGTC
Cuts at: 3117 3975 3117
Size: 858 4553
EaeI y'GGCC_r
Cuts at: 2996 3170 3561 3588 4933 2996
Size: 174 391 27 1345 3474
Fragments arranged by size:
3474 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2996 2996
Size: 5411
EarI CTCTTCn'nnn_
Cuts at: 219 486 1010 1657 2170 3434 3644 219
Size: 267 524 647 513 1264 210 1986
Fragments arranged by size:
1986 1264 647 524 513 267 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3619 3929 4075 5221 3619
Size: 310 146 1146 3809
Fragments arranged by size:
3809 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2423 2423
Size: 5411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 782 1349 1695 2117 3236 3668 4415 4683
Size: 418 567 346 422 1119 432 747 268
Cuts at: 4683 364
Size: 1092
Fragments arranged by size:
1119 1092 747 567 432 422 418 346
268
EcoO109I rG'GnC_Cy
Cuts at: 294 1088 1369 1925 2550 294
Size: 794 281 556 625 3155
Fragments arranged by size:
3155 794 625 556 281
EcoRI G'AATT_C
Cuts at: 704 2415 704
Size: 1711 3700
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3430 3430
Size: 5411
FspI TGC'GCA
Cuts at: 3192 3192
Size: 5411
HaeII r_GCGC'y
Cuts at: 118 194 200 206 2845 3093 4115 5094 118
Size: 76 6 6 2639 248 1022 979 435
Fragments arranged by size:
2639 1022 979 435 248 76 6 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 620 652 657 662 689 694 1180 1212 1270
Size: 32 5 5 27 5 486 32 58
Cuts at: 1270 1302 2145 2177 3253 3283 3285 3315 620
Size: 32 843 32 1076 30 2 30 2716
Fragments arranged by size:
2716 1076 843 486 58 32 32 32
32 30 30 27 5 5 5 2
HincII GTy'rAC
Cuts at: 275 2450 275
Size: 2175 3236
KasI G'GCGC_C
Cuts at: 190 196 202 2841 3089 190
Size: 6 6 2639 248 2512
Fragments arranged by size:
2639 2512 248 6 6
KpnI G_GTAC'C
Cuts at: 616 2431 616
Size: 1815 3596
MfeI C'AATT_G
Cuts at: 728 728
Size: 5411
MluI A'CGCG_T
Cuts at: 4844 4844
Size: 5411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 408 767 1170 4082 4266 408
Size: 359 403 2912 184 1553
Fragments arranged by size:
2912 1553 403 359 184
MscI TGG'CCA
Cuts at: 3172 3172
Size: 5411
MslI CAynn'nnrTG
Cuts at: 535 942 1541 1984 2285 3400 3527 535
Size: 407 599 443 301 1115 127 2419
Fragments arranged by size:
2419 1115 599 443 407 301 127
MspA1I CmG'CkG
Cuts at: 208 887 2522 2836 3196 4209 4454 208
Size: 679 1635 314 360 1013 245 1165
Fragments arranged by size:
1635 1165 1013 679 360 314 245
NaeI GCC'GGC
Cuts at: 189 195 201 3592 189
Size: 6 6 3391 2008
Fragments arranged by size:
3391 2008 6 6
NarI GG'CG_CC
Cuts at: 191 197 203 2842 3090 191
Size: 6 6 2639 248 2512
Fragments arranged by size:
2639 2512 248 6 6
NcoI C'CATG_G
Cuts at: 107 107
Size: 5411
NgoMIV G'CCGG_C
Cuts at: 187 193 199 3590 187
Size: 6 6 3391 2008
Fragments arranged by size:
3391 2008 6 6
NsiI A_TGCA'T
Cuts at: 2031 2031
Size: 5411
NspI r_CATG'y
Cuts at: 778 899 1639 2464 2964 3871 778
Size: 121 740 825 500 907 2318
Fragments arranged by size:
2318 907 825 740 500 121
PciI A'CATG_T
Cuts at: 895 1635 3867 895
Size: 740 2232 2439
Fragments arranged by size:
2439 2232 740
PflMI CCAn_nnn'nTGG
Cuts at: 1961 1961
Size: 5411
PfoI T'CCnGG_A
Cuts at: 1365 1365
Size: 5411
PmeI GTTT'AAAC
Cuts at: 2410 2410
Size: 5411
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4550 4582 4550
Size: 32 5379
PpuMI rG'GwC_Cy
Cuts at: 1369 1925 1369
Size: 556 4855
PshAI GACnn'nnGTC
Cuts at: 857 857
Size: 5411
PsiI TTA'TAA
Cuts at: 905 905
Size: 5411
PspOMI G'GGCC_C
Cuts at: 1087 1087
Size: 5411
PspXI vC'TCGA_Gb
Cuts at: 1443 1443
Size: 5411
PstI C_TGCA'G
Cuts at: 2458 3143 2458
Size: 685 4726
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5411
PvuII CAG'CTG
Cuts at: 2836 3196 2836
Size: 360 5051
RsrII CG'GwC_CG
Cuts at: 3606 3606
Size: 5411
SacI G_AGCT'C
Cuts at: 2425 2425
Size: 5411
SacII CC_GC'GG
Cuts at: 209 209
Size: 5411
SalI G'TCGA_C
Cuts at: 2448 2448
Size: 5411
SanDI GG'GwC_CC
Cuts at: 1925 1925
Size: 5411
SapI GCTCTTCn'nnn_
Cuts at: 3434 3644 3434
Size: 210 5201
SbfI CC_TGCA'GG
Cuts at: 2458 2458
Size: 5411
ScaI AGT'ACT
Cuts at: 1619 4928 1619
Size: 3309 2102
SfcI C'TryA_G
Cuts at: 33 1901 2454 3139 4132 4323 33
Size: 1868 553 685 993 191 1121
Fragments arranged by size:
1868 1121 993 685 553 191
SfoI GGC'GCC
Cuts at: 192 198 204 2843 3091 192
Size: 6 6 2639 248 2512
Fragments arranged by size:
2639 2512 248 6 6
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5411
SgrAI Cr'CCGG_yG
Cuts at: 193 199 193
Size: 6 5405
SmaI CCC'GGG
Cuts at: 2433 2433
Size: 5411
SmlI C'TyrA_G
Cuts at: 1443 1837 2566 3481 3973 4235 1443
Size: 394 729 915 492 262 2619
Fragments arranged by size:
2619 915 729 492 394 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5411
SphI G_CATG'C
Cuts at: 2464 2964 2464
Size: 500 4911
SspI AAT'ATT
Cuts at: 1242 1242
Size: 5411
StuI AGG'CCT
Cuts at: 349 349
Size: 5411
StyI C'CwwG_G
Cuts at: 107 1263 2545 2899 107
Size: 1156 1282 354 2619
Fragments arranged by size:
2619 1282 1156 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1016 1205 2382 2994 3127 3586 3795 1016
Size: 189 1177 612 133 459 209 2632
Fragments arranged by size:
2632 1177 612 459 209 189 133
TatI w'GTAC_w
Cuts at: 925 1617 4926 925
Size: 692 3309 1410
Fragments arranged by size:
3309 1410 692
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 232 264 1127 1159 4928 4960 232
Size: 32 863 32 3769 32 683
Fragments arranged by size:
3769 863 683 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3208 3208
Size: 5411
XbaI T'CTAG_A
Cuts at: 2442 2442
Size: 5411
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1270 1962 2906 1270
Size: 692 944 3775
Fragments arranged by size:
3775 944 692
XhoI C'TCGA_G
Cuts at: 1443 1443
Size: 5411
XmaI C'CCGG_G
Cuts at: 2431 2431
Size: 5411
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BmgBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI PacI PasI PmlI
PsrI SexAI SfiI SnaBI SrfI SwaI XmnI ZraI