Restriction map for pF1KB3024
##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AclI        AflII       AgeI        AocI        AscI        
AseI        AsnI        Asp700I     AviII       AvrII       
BbrPI       BciVI       BfrI        BlnI        BlpI        
Bpu1102I    BsiEI       BsiWI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrDI       BsrGI       BssSI       
Bsu36I      CelII       ClaI        CpoI        DraI        
EagI        EclXI       Eco52I      Eco81I      EcoT22I     
FseI        FspI        HindIII     HpaI        MfeI        
MluI        MseI        MunI        NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SalI        SapI        SfiI        SnaBI       SpeI        
SspBI       SspI        SwaI        Tru9I       XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII        2244  GACGTC               GACGT^C
Acc65I       3363  GGTACC               G^GTACC
AccIII       2336  TCCGGA               T^CCGGA
AflIII       1121  ACACGT               A^C[GA][CT]GT
AhdI         1179  GACTGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      3242  AGCGCT               AGC^GCT
Asp718I      3363  GGTACC               G^GTACC
AspEI        1179  GACTGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2713  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI         1561  GTCTTC               GAAGAC(2/6)
BcgI         1840  CGAGGGCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         3149  TGATCA               T^GATCA
BpuAI        1561  GTCTTC               GAAGAC(2/6)
BseAI        2336  TCCGGA               T^CCGGA
BsmBI        2440  GAGACG               CGTCTC(1/5)
BspEI        2336  TCCGGA               T^CCGGA
Bst1107I     1155  GTATAC               GTA^TAC
BstBI         716  TTCGAA               TT^CGAA
BstZ17I      1155  GTATAC               GTA^TAC
DrdI         1552  GACCTCAGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1179  GACTGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     3242  AGCGCT               AGC^GCT
EcoRV         517  GATATC               GAT^ATC
FbaI         3149  TGATCA               T^GATCA
HincII       1876  GTCAAC               GT[CT]^[GA]AC
HindII       1876  GTCAAC               GT[CT]^[GA]AC
KpnI         3363  GGTACC               GGTAC^C
KspI         2730  CCGCGG               CCGC^GG
MroI         2336  TCCGGA               T^CCGGA
NdeI         2630  CATATG               CA^TATG
NspV          716  TTCGAA               TT^CGAA
PflFI        2713  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacII        2730  CCGCGG               CCGC^GG
SbfI          510  CCTGCAGG             CCTGCA^GG
SexAI        3338  ACCTGGT              A^CC[AT]GGT
SfuI          716  TTCGAA               TT^CGAA
SgrAI        2977  CGCCGGCG             C[GA]^CCGG[CT]G
SmlI           78  CTTGAG               C^T[CT][GA]AG
SphI         2155  GCATGC               GCATG^C
Sse8387I      510  CCTGCAGG             CCTGCA^GG
StuI          696  AGGCCT               AGG^CCT
Tth111I      2713  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

Alw44I        366  GTGCAC               G^TGCAC
             1720  GTGCAC              
ApaLI         366  GTGCAC               G^TGCAC
             1720  GTGCAC              
BalI         2540  TGGCCA               TGG^CCA
             3227  TGGCCA              
BbeI         1165  GGCGCC               GGCGC^C
             1606  GGCGCC              
BglI          148  GCCCCCCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2642  GCCTTCTGGGC         
BglII         731  AGATCT               A^GATCT
             1397  AGATCT              
BsaAI         538  TACGTG               [CT]AC^GT[GA]
             2605  TACGTG              
BsaBI        2307  GATCCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2424  GATGCGGATC          
DraIII       1723  CACACGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1866  CACCCAGTG           
EarI         1501  GAAGAG               CTCTTC(1/4)
             3490  GAAGAG              
EcoNI         435  CCTCTACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              673  CCTGTCACAGG         
EcoRI        1592  GAATTC               G^AATTC
             3214  GAATTC              
KasI         1165  GGCGCC               G^GCGCC
             1606  GGCGCC              
Ksp632I      1501  GAAGAG               CTCTTC(1/4)
             3490  GAAGAG              
MamI         2307  GATCCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2424  GATGCGGATC          
MluNI        2540  TGGCCA               TGG^CCA
             3227  TGGCCA              
MscI         2540  TGGCCA               TGG^CCA
             3227  TGGCCA              
NaeI         1015  GCCGGC               GCC^GGC
             2978  GCCGGC              
NarI         1165  GGCGCC               GG^CGCC
             1606  GGCGCC              
NcoI          264  CCATGG               C^CATGG
              290  CCATGG              
NgoMIV       1015  GCCGGC               G^CCGGC
             2978  GCCGGC              
PflMI        2037  CCAGTGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2945  CCATCAAGTGG         
ScaI         1926  AGTACT               AGT^ACT
             3875  AGTACT              
SfoI         1165  GGCGCC               GGC^GCC
             1606  GGCGCC              
TaqI          717  TCGA                 T^CGA
             3941  TCGA                
TfiI          113  GATTC                G^A[AT]TC
             3194  GATTC               
TthHB8I       717  TCGA                 T^CGA
             3941  TCGA                
Van91I       2037  CCAGTGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2945  CCATCAAGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AluI        AlwI        AlwNI       ApaI        
ApoI        AspHI       AvaI        AvaII       BamHI       
BanI        BanII       BbvCI       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaHI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssHII      BssKI       BstAPI      BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoO65I     EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        StyI        TseI        Tsp45I      
Tsp509I     TspRI       XcmI        XhoII       XmaCI       
XmaI        
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